Quick Blunting Kit from New England Biolabs

Product Specs
ItemQuick Blunting Kit
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberE1201S
Quantity20 reactions
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) High-Throughput Mutant Screening in Vibrio cholerae via Transposon SequencingCold Spring Harb ProtocOctober 3, 2023Jacob Bourgeois, Andrew Camillimolten agarose. QIAquick PCR Purification Kit (QIAGEN 28104) Quick Blunting Kit (New England Biolabs E1201L) TAE Terminal deoxynucleotidyl transferase (TdT), 5× Reaction buffer (Promega M828C) Terminal

(2) Polycomb repressive complex 2 binds and stabilizes NANOG to suppress differentiation-related genes to promote self-renewaliScienceJuly 21, 2023Yeh DW, Liu C, Hernandez JC, Tahara SM, Tsukamoto H, Machida K.,New England BioLabs,M0202L NEBuilder® HiFi DNA Assembly Master Mix,New England BioLabs,E2621L Quick Blunting™ Kit,New England BioLabs,E1201L "GenClone Fetal Bovine Serum, Heat Inactivated",Genesee Scientific,25-514H "Dulbecco’s modified Eagle’s

(3) In vitro assembly of plasmid DNA for direct cloning in Lactiplantibacillus plantarum WCSF1PLOS ONEFebruary 16, 2023Marc Blanch-Asensio, Sourik Dey, Shrikrishnan SankaranArt. No. E5520S). For plasmid circularization, the Quick Blunting Kit (NEB GmbH, Germany, Art. No. E1201S) and the T4 DNA Ligase enzyme (NEB GmbH, Germany, Art. No. M0318S) were used. L. plantarum WCFS1 electrocompetent

(4) A NPAS4-NuA4 complex couples synaptic activity to DNA repairNatureFebruary 1, 2023Pollina EA, Gilliam DT, Landau AT, Lin C, Pajarillo N, Davis CP, Harmin DA, Yap EL, Vogel IR, Griffith EC, et al.Triton X-100). At room temperature, nuclei were blunted using a Quick Blunting kit (New England Biolabs, E1201) for 1 h. Following two washes with CutSmart wash buffer, adapters were ligated onto free DNA ends in

(5) Resolving the structure of phageâ€“bacteria interactions in the context of natural diversityNature CommunicationsDecember 1, 2022Kathryn M. Kauffman, William K. Chang, Julia M. Brown, Fatima A. Hussain, Joy Yang, Martin F. Polz, Libusha Kellyconstructs were prepared from sheared DNA as follows: end repair of sheared DNA (NEB, Quick Blunting Kit, E1201L), 0.72×/0.21× dSPRI (AMPure XP SPRI Beads) size selection to enrich for ~300 bp sized fragments, ligation

(6) Long-term hepatitis B virus infection of rhesus macaques requires suppression of host immunityNature CommunicationsDecember 1, 2022Sreya Biswas et al.(ThermoFisher, Cat# ER0641), end-blunted using the Quick Blunting Kit (New England Biolabs, Ipswich, MA, Cat# E1201L), and dephosphorylated. In parallel, PB511-hNTCP was digested with fast-digest PteI (ThermoFisher, Cat#

(7) Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavageNature CommunicationsDecember 1, 2022Stephan Riesenberg, Nelly Helmbrecht, Philipp Kanis, Tomislav Maricic, Svante Pääbosheared fragments were blunted for 30 min at 20 °C with the Quick Blunting Kit (New Engand Biolabs, E1201L), purified with SPRI beads28, adapter-ligated for 30 min at 20 °C with the Quick Ligation Kit (New England Biolabs

(8) Precision digital mapping of endogenous and induced genomic DNA breaks by INDUCE-seqNature CommunicationsDecember 1, 2022Felix M. Dobbs, Patrick van Eijk, Mick D. Fellows, Luisa Loiacono, Roberto Nitsch, Simon H. Reedthree times in 1x CutSmart® Buffer (NEB, B7204S) and blunt-end repaired using NEB Quick Blunting Kit (E1201L) + 100 µg/mL BSA in a final volume of 50 µL at rt for one hour. Cells were then washed three times in

(9) The SPARC complex defines RNAPII promoters in Trypanosoma bruceieLifeSeptember 28, 2022Desislava P Staneva, Stefan Bresson, Tatsiana Auchynnikava, Christos Spanos, Juri Rappsilber, A Arockia Jeyaprakash, David Tollervey, Keith R Matthews, Robin C Allshire(plasmid),Invitrogen,,Used to generate the SET27 rescue cell line Commercial assay or kit,Quick Blunting Kit,NEB,Cat #E1201L, Commercial assay or kit,Klenow Fragment (3′→5′ exo-),NEB,Cat #M0212S, Commercial assay or kit,NEXTflex-96

(10) Chronic Granulomatous Disease-Like Presentation of a Child with Autosomal Recessive PKCÎ´ DeficiencyJournal of Clinical ImmunologyAugust 1, 2022Anna-Lena Neehus, Karen Tuano, Tom Le Voyer, Sarada L. Nandiwada, Kruthi Murthy, Anne Puel, Jean-Laurent Casanova, Javier Chinen, Jacinta BustamanteMachery-Nagel). For the deletion of exon 5, blunting and ligation were performed with the Quick Blunting (#E1201S, New England Biolabs) and Quick Ligation (#M2200S, New England Biolabs) kits. Plasmids were amplified

(11) c-JUN inhibits mTORC2 and glucose uptake to promote self-renewal and obesityiScienceJune 17, 2022Raphael Serna, Ambika Ramrakhiani, Juan Carlos Hernandez, Chia-Lin Chen, Chad Nakagawa, Tatsuya Machida, Ratna B. Ray, Xiaohang Zhan, Stanley M. Tahara, Keigo MachidaStellar™ Competent Cells,Takara,636766 Q5® High-Fidelity DNA Polymerase,NEB,M0491L T4 DNA Ligase,NEB,M0202L Quick Blunting™ Kit,NEB,E1201L "GenClone Fetal Bovine Serum, Heat Inactivated",Genesee Scientific,25-514H "Dulbecco’s modified Eagle’s

(12) Imaging-Based Screening of Deubiquitinating Proteases Identifies Otubain-1 as a Stabilizer of c-MYCCancers (Basel)February 4, 2022Shannon E. Moree, Laure Maneix, Polina Iakova, Fabio Stossi, Ergun Sahin, Andre CaticQiagen, Germantown, MD, USA), blunted at room temperature using the Quick Blunting Kit from NEB (Cat. E1201), and heat inactivated for 10 min at 70 °C. The ZsGreen1 gene from pRetroX-IRES-ZsGreen1 (TakaraBio,

(13) Genome-scale sequencing and analysis of human, wolf, and bison DNA from 25,000-year-old sedimentCurrent BiologyAugust 23, 2021Pere Gelabert, Susanna Sawyer, Anders BergstrÃ¶m, Ashot Margaryan, Thomas C. Collin, Tengiz Meshveliani, Anna Belfer-Cohen, David Lordkipanidze, Nino Jakeli, Zinovi Matskevich, et al.(75 cycle),Illumina,TG-160-2005 DNeasy Blood and Tissue kit,QIAGEN,69506 Quick Blunting system,NEB,E1201S Quick Ligation kit,NEB,M2200S Expand Long Template PCR System,Roche,11681834001 AccuPrimePfx DNA

(14) An early cell shape transition drives evolutionary expansion of the human forebrainCellApril 15, 2021Benito-Kwiecinski S, Giandomenico SL, Sutcliffe M, Riis ES, Freire-Pritchett P, Kelava I, Wunderlich S, Martin U, Wray GA, McDole K, et al.restriction enzymes AflI (NEB, R0520S) and AscI (NEB, R0558S) and blunted with the Quick Blunting Kit (NEB, E1201). The resulting intermediate plasmid was then digested with MluI and KpnI and ligated to the cassette

(15) Ubiquitylation of MYC couples transcription elongation with double-strand break repair at active promotersMolecular cellFebruary 18, 2021Endres T, Solvie D, Heidelberger JB, Andrioletti V, Baluapuri A, Ade CP, Muhar M, Eilers U, Vos SM, Cramer P, et al."ABsolute QPCR Mix, SYBR Green, no ROX",Thermo Fischer Scientific,Cat#AB-1158/B Quick Blunting Kit,New England Biolabs,Cat#E1201L "T4 DNA Ligase, conc.",New England Biolabs,Cat#M0202M "T4 RNA Ligase 2, truncated",New England Biolabs,Cat#M0242L NEBNext High-Fidelity 2X PCR Master Mix

(16) High-resolution, ultra-sensitive and quantitative DNA double-strand break labeling in eukaryotic cells using i-BLESSNature ProtocolsFebruary 1, 2021Anna Biernacka, Magdalena Skrzypczak, Yingjie Zhu, Philippe Pasero, Maga Rowicka, Krzysztof GinalskiNEBNext® FFPE DNA Repair Mix (New England Biolabs, cat.no. M6630L) Quick Blunting Kit (New England Biolabs, cat. no. E1201L) BSA (20 mg/mL, New England Biolabs, cat. no. B9000S) NEBNext® dA-Tailing Module (New England Biolabs,

(17) Caspase-1 Engages Full-Length Gasdermin D through Two Distinct Interfaces That Mediate Caspase Recruitment and Substrate CleavageImmunityJuly 1, 2020Zhonghua Liu, Chuanping Wang, Jie Yang, Yinghua Chen, Bowen Zhou, Derek W. Abbott, Tsan Sam Xiaocytotoxicity assay kit,Thermo Fisher Scientific,Cat# 88953 Quick Blunting Kit,New England Biolabs,Cat# E1201S T4 DNA Ligase,New England Biolabs,Cat# M0202S Phusion High-Fidelity DNA Polymerase,New England Biolabs,Cat#

(18) Overexpression of AmpC Promotes Bacteriophage Lysis of Ampicillin-Resistant Escherichia coliFrontiers in MicrobiologyJanuary 8, 2020Shuang Wang et al.waves into random fragments of 150 to 2500 bp. Following end-filling using Quick BluntingTM Kit (NEB, #E1201L) and phosphorylation using Alkaline Phosphatase (NEB, #M0290S), the purified random phage DNA fragments

(19) Simultaneous precise editing of multiple genes in human cellsNucleic Acids ResearchNovember 4, 2019Stephan Riesenberg, Manjusha Chintalapati, Dominik Macak, Philipp Kanis, Tomislav Maricic, Svante Pääboends were made blunt for 30 min at room temperature with the Quick Blunting Kit (New Engand Biolabs, E1201L), purified with SPRI beads (28), adapter-ligated for 30 min at room temperature with the Quick Ligation

(20) Crystal Structures of the Full-Length Murine and Human Gasdermin D Reveal Mechanisms of Autoinhibition, Lipid Binding, and OligomerizationImmunityJuly 1, 2019Zhonghua Liu, Chuanping Wang, Jie Yang, Bowen Zhou, Rui Yang, Rajesh Ramachandran, Derek W. Abbott, Tsan Sam Xiaocytotoxicity assay kit,Thermo Fisher Scientific,Cat#88953 Quick Blunting Kit,New England Biolabs,Cat#E1201S T4 DNA Ligase,New England Biolabs,Cat#M0202S Phusion High-Fidelity DNA Polymerase,New England Biolabs,Cat#M0530L Gibson Assembly Cloning Kit

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