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RNA Methylation Analysis - Global m6A Quantification
RNA Methylation Analysis - Global m6A Quantification from EpiGentek
Be the first to write a review!
Citations:
(59)
Supplier Page
Supplier Page from
EpiGentek for
RNA Methylation Analysis - Global m6A Quantification
Description
Quantitate total methylated adenosine levels in RNA on a global scale.
Includes:
Antibody-based processing of samples, Accurate readout of OD values, Calculation of total global m6A levels
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
RNA Methylation Analysis - Global m6A Quantification
Company
EpiGentek
Price
Supplier Page
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Catalog Number
Global m6A
Quantity
EA
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EpiGentek
110 Bi County Blvd. Ste 122
Farmingdale
,
NY
11735
United States
Phone
: 1 877 EPIGENTEK (374-4368)
International: 1 631 755-0888
Fax (General): 1 631 815-6729
Fax (Orders): 1 718 484-3956
Email: info@epigentek.com
sales@epigentek.com
services@epigentek.com
support@epigentek.com
Company Profile
Website:
www.epigentek.com
Citations (59)
(1)
The m6A regulator KIAA1429 stabilizes RAB27B mRNA and promotes the progression of chronic myeloid leukemia and resistance to targeted therapy
Genes & Diseases
March 1, 2024
Fangyi Yao, Fangmin Zhong, Junyao Jiang, Ying Cheng, Shuai Xu, Jing Liu, Jin Lin, Jing Zhang, Shuqi Li, Meiyong Li,
et al.
EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric
) (
Epigentek
, USA) was utilized to
measure
the
global m6A
levels in mRNA following the manufacturer's protocol. A total of 200 ng of polyA+ mRNA was used for
(2)
Transcriptome-wide N6-methyladenosine methylation profile of atherosclerosis in mice
BMC Genomics
December 14, 2023
Xinbin Zheng, Bo Zhou, Yuzhen Li, Hengren Zhong, Zhengxin Huang, Minhua Gu
with over-dose anesthetic and collected the whole aorta for further studies. m6A quantification The
global m6A
levels were detected by EpiQuik™ m6A RNA Methylation Quantification Kit (
EpiGentek
, NY, USA) according
(3)
Low RNA stability signifies strong expression regulatability of tumor suppressors
Nucleic Acids Research
November 27, 2023
Xinlei Gao, Yang Yi, Jie Lv, Yanqiang Li, Kulandaisamy Arulsamy, Sahana Suresh Babu, Ivone Bruno, Lili Zhang, Qi Cao, Kaifu Chen
2−ΔΔCt method with the Ct values normalized using GAPDH as an internal control. Quantification of
global m6A
methylation level in RNA The m6A methylation levels in PrEC and C4-2 cells were measured using EpiQuik
(4)
METTL3 alters capping enzyme expression and its activity on ribosomal proteins
bioRxiv
November 26, 2023
Daniel del Valle-Morales, Giulia Romano, Patricia Le, Michela Saviana, Rachel Brown, Lavender Micalo, Howard Li, Alessandro La La Ferlita, Giovanni Nigita, Patrick Nana-Sinkam,
et al.
motifs were generated using WebLogo (https://weblogo.berkeley.edu/) with the default settings [36].
Global m6A
quantification
Global m6A
levels were assayed using
EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric
(5)
ALKBH5-mediated CHAC1 depletion promotes malignant progression and decreases cisplatin-induced oxidative stress in gastric cancer
Cancer Cell International
November 25, 2023
Chunting Chen, Ertao Zhai, Yinan Liu, Yan Qian, Risheng Zhao, Yan Ma, Jianqiu Liu, Zhixin Huang, Jianhui Chen, Shirong Cai
isolated for
qPCR
analysis. See Table S2 for
quantitative PCR
primers. m6A quantification The change of
global m6A
levels in mRNA was measured by
EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric
) (
EpigenTek
)
(6)
Comprehensive Transcriptomic Profiling of m6A Modification in Age-Related Hearing Loss
Biomolecules
October 18, 2023
Menglong Feng, Xiaoqing Zhou, Yaqin Hu, Juhong Zhang, Ting Yang, Zhiji Chen, Wei Yuan
mRNA primers used in our study are listed in Table A1. 2.4. m6A Quantification The alterations in
global m6A
levels in total mRNA were detected with an
EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric
)
(7)
LncRNA FTO-IT1 promotes glycolysis and progression of hepatocellular carcinoma through modulating FTO-mediated N6-methyladenosine modification on GLUT1 and PKM2
Journal of Experimental & Clinical Cancer Research
October 16, 2023
Fan Wang, Yuhang Hu, Hongda Wang, Ping Hu, Hewei Xiong, Zhu Zeng, Shengbo Han, Decai Wang, Jie Wang, Yong Zhao,
et al.
2.5 volumes of 100% ethanol at -80 °C overnight. m6A enrichment was determined by
qPCR
analysis. The
global m6A
levels in mRNA was measured with
EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric
) (
Epigentek
,
(8)
METTL3 Promotes the Differentiation of Goat Skeletal Muscle Satellite Cells by Regulating MEF2C mRNA Stability in a m6A-Dependent Manner
International Journal of Molecular Sciences
September 14, 2023
Sen Zhao, Jiaxue Cao, Yanjin Sun, Helin Zhou, Qi Zhu, Dinghui Dai, Siyuan Zhan, Jiazhong Guo, Tao Zhong, Linjie Wang,
et al.
the proliferative phase (GM-3, GM-5) and differentiation phase (DM-2, DM-5) using RNAiso Plus. The
global m6A
methylation level of RNA was detected using the
m6A
RNA Methylation Quantification Kit (
Epigentek
, Farmingdale,
(9)
ALKBH5 promotes hypopharyngeal squamous cell carcinoma apoptosis by targeting TLR2 in a YTHDF1/IGF2BP2-mediated manner
Cell death discovery
August 23, 2023
Ye J, Wu Y, Chen Y, Ren Y, Jiang X, Dong Z, Zhang J, Jin M, Chen X, Wang Z,
et al
.
were kept in a humidified 37 °C and 5% CO2 environment, and the medium was changed every three days.
Global m6A
measurement
Global m6A
levels in mRNA were measured with an
EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric
(10)
In vitro study of piwi interaction RNA-31106 promoting breast carcinogenesis by regulating METTL3-mediated m6A RNA methylation
Translational Cancer Research
June 30, 2023
Shengchao Huang, Baoying Chen, Pu Qiu, Zeming Yan, Zhongzeng Liang, Kangwei Luo, Baoyi Huang, Haiping Jiang
of m6A RNA methylation Total RNA was isolated and purified from
breast cancer
cell lines, and the
global m6A
RNA methylation levels were measured using the EpiQuik m6A RNA methylation quantitative kit (
EpigenTek
,
(11)
Comprehensive analysis of differences in N6-methyladenosine RNA methylomes in Helicobacter pylori infection
Frontiers in Cell and Developmental Biology
June 7, 2023
Huan Li, Jiahui Lin, Sha Cheng, Jingshu Chi, Ju Luo, Yu Tang, Wenfang Zhao, Yufeng Shu, Xiaoming Liu, Canxia Xu
EpiQuik m6A Methylation Quantification Kit (
Epigentek
,
P-9005
-96, United States) was used to
measure
the
global m6A
enrichment of mRNA. 200 ng RNA was coated in
assay
wells from each sample. The m6A levels are colorimetrical
(12)
VIRMA promotes nasopharyngeal carcinoma, tumorigenesis, and metastasis by upregulation of E2F7 in an m6A-dependent manner
Journal of Biological Chemistry
May 1, 2023
Zi-Qi Zheng, Zhuo-Hui Huang, Ye-Lin Liang, Wei-Hong Zheng, Cheng Xu, Zhi-Xuan Li, Na Liu, Pan-Yang Yang, Ying-Qin Li, Jun Ma,
et al.
extracted using TRIzol and subjected to mRNA separation using a GenElute mRNA Miniprep Kit (Sigma).
Global m6A
levels in polyadenylated RNAs were measured using an EpiQuik m6A RNA Methylation Quantification Kit
(13)
m6A methyltransferase METTL3 programs CD4+ T-cell activation and effector T-cell differentiation in systemic lupus erythematosus
Molecular Medicine
April 3, 2023
Shuang Lu, Xingyu Wei, Huan Zhu, Zhi Hu, Meiling Zheng, Jiali Wu, Cheng Zhao, Shuang Yang, Delong Feng, Sujie Jia,
et al.
quantification Total RNA was isolated using TRIzol reagent according to the manufacturer’s instructions.
Global m6A
levels in total RNA were evaluated by the EpiQuick™ m6A RNA Methylation Quantification Kit (Colorimetric)
(14)
m6A reader IGF2BP1 accelerates apoptosis of high glucose-induced vascular endothelial cells in a m6A-HMGB1 dependent manner
PeerJ
March 27, 2023
Anru Liang, Jianyu Liu, Yanlin Wei, Yuan Liao, Fangxiao Wu, Jiang Ruan, Junjun Li
Image-Pro Plus (IPP) 6.0 software (Media Cybernetics, Rockville, MD, USA). m6A quantification
assay
The
global m6A
levels in mRNA were measured by EpiQuik m6A RNA Methylation Quantification Kit (Colorimetrically;
Epigentek
,
(15)
m6A eraser FTO modulates autophagy by targeting SQSTM1/P62 in the prevention of canagliflozin against renal fibrosis
Frontiers in Immunology
January 4, 2023
Youjing Yang, Qianmin Li, Yi Ling, Linxin Leng, Yu Ma, Lian Xue, Guoyuan Lu, Yue Ding, Jianzhong Li, Shasha Tao
Corresponding secondary antibodies were added and incubated for 1 h. 2.15 m6A quantification The levels of
global m6A
in mRNA were measured by using an
EpiQuik m6A RNA Methylation Quantification kit (Colorimetric
) (
EpiGentek
,
(16)
LncNAP1L6 activates MMP pathway by stabilizing the m6A-modified NAP1L2 to promote malignant progression in prostate cancer
Cancer gene therapy
January 1, 2023
Zheng Y, Qi F, Li L, Yu B, Cheng Y, Ge M, Qin C, Li X.
With the application of RNA total m6A quantification (
P-9008
-48-E,
Epigentek
, USA), we discovered that
global m6A
levels were decresed when
METTL14
was downregulated (Fig. 2O). Further, MeRIP attested that
METTL14
(17)
Transcriptome-wide m6A methylation in natural yellow leaf of Catalpa fargesii
Frontiers in plant science
January 1, 2023
Zhang Y, Wang J, Ma W, Lu N, Fu P, Yang Y, Zhao L, Hu J, Qu G, Wang N.
selection were performed to
measure
the global change of the m6A modification level. The change of
global m6A
levels in mRNA was measured by EpiQuik m6A RNA Methylation Quantification Kit (
EpiGentek
,
P-9005
, NY,
(18)
LRPPRC facilitates tumor progression and immune evasion through upregulation of m(6)A modification of PD-L1 mRNA in hepatocellular carcinoma
Frontiers in immunology
January 1, 2023
Wang H, Tang A, Cui Y, Gong H, Li H.
for eluting RNA.
RT-qPCR
was implemented on the
immunoprecipitated
RNA for detect the enrichment.
Global m6A
quantification
Global m6A
level was measured utilizing EpiQuik™ m6A RNA Methylation Quantification
(19)
ALKBH5 promotes PD-L1-mediated immune escape through m6A modification of ZDHHC3 in glioma
Cell Death Discovery
December 24, 2022
Wenhui Tang, Ningbo Xu, Jian Zhou, Zhenyan He, Cameron Lenahan, Chenyang Wang, Huangyi Ji, Boyang Liu, Yujiao Zou, Huijun Zeng,
et al.
Fluorescence
-minus-one, unstained, and single-stained cells were also used to set gates. m6A quantification The
global m6A
levels in total RNAs were measured using the EpiQuik m6A RNA Methylation Quantification Kit (
Epigentek
,
(20)
A programmable system to methylate and demethylate N6-methyladenosine (m6A) on specific RNA transcripts in mammalian cells
Journal of Biological Chemistry
November 1, 2022
Chen Chang, Gang Ma, Edwin Cheung, Andrew P. Hutchins
substrate (Bio-Rad) according to the manufacturer's instructions. Whole transcriptome m6A measurements
Global m6A
/m in total RNA was quantified by the EpiQuik m6A/m RNA Methylation Quantification Kit (
Epigentek
Group)
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RNA Methylation Analysis - Global m6A Quantification from EpiGentek
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