TNT T7/SP6 Coupled Reticulocyte Lysate System from Promega

Product Specs
ItemTNT T7/SP6 Coupled Reticulocyte Lysate System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberL5020
Quantity40 reactions
SystemT7/T3 Coupled Reticulocyte Lysate System

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Autism-associated protein POGZ controls ESCs and ESC neural induction by association with esBAFMolecular autismJune 1, 2022Sun X, Cheng L, Sun Y.were synthesized using the TNT coupled reticulocyte lysate system according to the manual (Promega, L5020, USA). Briefly, 1 μg of circular pCS2 version of plasmids was added directly to the TNT lysates and

(2) Phosphorylation of islet-1 serine 269 by CDK1 increases its transcriptional activity and promotes cell proliferation in gastric cancerMolecular MedicineDecember 1, 2021Qiong Shi, Xiaomei Ni, Ming Lei, Quansong Xia, Yan Dong, Qiao Zhang, Weiping WangBiotin-labeled wild-type CDK1 was synthesized using the TNT Quick Coupled Transcription/Translation System (L5020, Promega, Madison, WI, USA) (Wang et al. 2016). Immunohistochemistry (IHC) All human GC tissue specimens

(3) Control of ribosomal protein synthesis by the Microprocessor complexScience signalingFebruary 23, 2021Jiang X, Prabhakar A, Van der Voorn SM, Ghatpande P, Celona B, Venkataramanan S, Calviello L, Lin C, Wang W, Black BL, et al.with Drosha RBD protein, which was synthesized in vitro with a reticulocyte lysate system (Promega, L5020), for 2 h at room temperature in binding buffer (50 mM Tris-Cl, pH 7.5; 100 mM KCl; 2.5 mM MgCl2; 100

(4) ADNP promotes neural differentiation by modulating Wnt/β-catenin signalingNature communicationsJune 12, 2020Sun X, Peng X, Cao Y, Zhou Y, Sun Y.assay Protein–protein interaction assay was performed using a rabbit reticulocyte lysate system (Promega, L5020, USA)38. Tagged-ADNP, tagged-ADNP mutants, tagged-β-catenin, and tagged-β-catenin mutants were synthesized

(5) Endoplasmic reticulum stress actively suppresses hepatic molecular identity in damaged liverMolecular Systems BiologyMay 14, 2020Vanessa Dubois et al."Simple Western, WES system",ProteinSimple,N/A TnT® Quick Coupled Transcription/Translation System,Promega,Cat# L5020 iBright™ CL1500 Imaging System,Thermo Fischer Scientific,A44240

(6) ADNP Controls Gene Expression Through Local Chromatin Architecture by Association With BRG1 and CHD4Frontiers in cell and developmental biologyJanuary 1, 2020Sun X, Yu W, Li L, Sun Y.proteins were synthesized using the TNT coupled reticulocyte lysate system according to the manual (L5020, Promega, United States). Briefly, 1 μg of a circular PCS2-version of plasmid DNA was added directly

(7) Mga Modulates Bmpr1a Activity by Antagonizing Bs69 in ZebrafishFrontiers in Cell and Developmental BiologySeptember 28, 2018Xiaoyun Sun, Ji Chen, Yanyong Zhang, Mumingjiang Munisha, Scott Dougan, Yuhua Sunproteins were synthesized using the TnT coupled reticulocyte lysate system according to the manual (L5020, Promega, United States). Briefly, 1 μg of circular PCS2- version of plasmid were added directly to

(8) ISL-1 promotes pancreatic islet cell proliferation by forming an ISL-1/Set7/9/PDX-1 complexCell CycleDecember 17, 2015Zhe Yang, Qiao Zhang, Qin Lu, Zhuqing Jia, Ping Chen, Kangtao Ma, Weiping Wang, Chunyan Zhouwild-type Set7/9 or PDX-1 were synthesized using the TNT Quick Coupled Transcription/Translation System (L5020, Promega, Madison, WI, USA). IGF-1 treatment NIT-1 cell proliferation was stimulated by insulin-like

(9) Regulation of c-Myc protein stability by proteasome activator REGγCell death and differentiationJune 1, 2015Li S, Jiang C, Pan J, Wang X, Jin J, Zhao L, Pan W, Liao G, Cai X, Li X, et al.all purchased from Sigma (St. Louis, MO, USA). Dual-Luciferase Reporter System Kit (E1501) and TNT (L5020) were purchased from Promega (Madison, WI, USA). Cell culture and transfections. Human embryonic kidney

(10) PRDM16 binds MED1 and controls chromatin architecture to determine a brown fat transcriptional programGenes & DevelopmentFebruary 1, 2015Matthew J. Harms, Hee-Woong Lim, Yugong Ho, Suzanne N. Shapira, Jeff Ishibashi, Sona Rajakumari, David J. Steger, Mitchell A. Lazar, Kyoung-Jae Won, Patrick Seale(Kajimura et al. 2008). S35-labeled MED1 was prepared using the TNT reticulocyte lysate kit (Promega, L5020). Equal amounts of GST-PRDM16 proteins were incubated overnight at 4°C with in vitro translated MED1

(11) Expression of Caytaxin Protein in Cayman Ataxia Mouse Models Correlates with Phenotype SeverityPLoS ONENovember 30, 2012Kristine M. Sikora, LaGina M. Nosavanh, Prameela Kantheti, Margit Burmeister, Michael Hortschmanufacturer’s protocol using the TnT® T7/SP6 Coupled Reticulocyte Lysate System (Promega, Cat. No. L5020). 7 µL of a 25 µL reaction was loaded on 10% SDS-PAGE gels. Additional reagents included EasyTag™ L-[35S]-Methionine,

(12) Shp2 Controls Female Body Weight and Energy Balance by Integrating Leptin and Estrogen SignalsMolecular and Cellular BiologyMay 15, 2012Zhao He et al.protein by TNT coupled reticulocyte lysate systems (cell-free protein expression, catalog nos. L5061 and L5020; Promega). The translation products were mixed with or without estrodial17␤ and subjected to immunoprecipitation assay

(13) Measurement of protein farnesylation and geranylgeranylation in vitro, in cultured cells and in biopsies, and the effects of prenyl transferase inhibitorsNature protocolsOctober 27, 2011Berndt N, Sebti SM.(in vitro transcription-translation-prenylation) • TnT reticulocyte lysate system (Promega, cat. no. L4600, L4610, L4950, L5010, L5020) M CRITICAL Which system to purchase depends on the nature of the RNA polymerase promoter used in the

(14) The immunophilin-like protein XAP2 is a negative regulator of estrogen signaling through interaction with estrogen receptor αPloS oneJanuary 1, 2011Cai W, Kramarova TV, Berg P, Korbonits M, Pongratz I.performed using rabbit reticulocyte lysate system (TNT® T7/SP6 Coupled Reticulocyte Lysate System, Cat#L5020, Promega), according to manufacturer's insctructions. In brief, purified XAP2-pSG5, ERα-pSG5 vector

(15) A possible inflammatory role of twist1 in human white adipocytesDiabetesMarch 1, 2010Amanda T Pettersson, Jurga Laurencikiene, Niklas Mejhert, Erik Näslund, Anne Bouloumié, Ingrid Dahlman, Peter Arner, Mikael Rydénconfirmed by in vitro translation using a TnT T7/SP6 Coupled Reticulocyte Lysate System (catalog number L5020; Promega). Gene reporter assays were performed in undifferentiated 3T3-L1 cells transfected with Lipofectamine

(16) The truncated Hoxa1 protein interacts with Hoxa1 and Pbx1 in stem cellsJournal of Cellular BiochemistryFebruary 15, 2009Cristina C. Fernandez, Lorraine J. GudasGE Healthcare) using TNT Quick Coupled Transcription/Translation System (Promega, Madison, WI, cat # L5020) according to the manufacturer’s protocol. Protein products (2 μL) were resolved by SDS-PAGE. The gel

(17) alpha-Actinin interacts with rapsyn in agrin-stimulated AChR clusteringMolecular brainDecember 3, 2008Dobbins GC, Luo S, Yang Z, Xiong WC, Mei L.presence of [35S]-labeled methionine using TnT T7/SP6 Coupled Reticulocyte Lysate System (Promega, L5020) [53]. [35S]-labeled rapsyn was then incubated with immobilized GST-α-actinin constructs in the binding

(18) IkappaB kinase alpha-mediated derepression of SMRT potentiates acetylation of RelA/p65 by p300Molecular and Cellular BiologyJanuary 15, 2006Jamie E. Hoberg, Anita E. Popko, Catherine S. Ramsey, Marty W. MayoTNT T7/SP6 coupled reticulocyte lysate system according to the manufacturer’s specifications (catalog no. L5020; Promega). One-tenth of the 35S-labeled RelA/p65 was mixed with GST protein or various GST-SMRT proteins

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