TNT T7 Coupled Wheat Germ Extract System from Promega

Product Specs
ItemTNT T7 Coupled Wheat Germ Extract System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberL4140
Quantity40 reactions
SystemWheat Germ Extract
Molecular Weight~74kDa

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Anti-human immunodeficiency virus-1 activity of MoMo30 protein isolated from the traditional African medicinal plant Momordica balsaminaVirology JournalMarch 22, 2023Mahfuz Khan, Amad Diop, Erick Gbodossou, Peng Xiao, Morgan Coleman, Kenya De Barros, Hao Duong, Vincent C. Bond, Virginia Floyd, Kofi Kondwani, et al.germ coupled transcription/translation system (TNT T7 coupled Wheat Germ Extract System Promega Cat# L4140) according to the manufacturer's recommendations with the following modification. Instead of adding

(2) Centrosome function is critical during terminal erythroid differentiationThe EMBO journalJuly 18, 2022Tátrai P, Gergely F.generate IVT. Non‐radioactive IVT was performed using the TNT T7‐coupled wheat germ extract system (L4140, Promega), according to the manufacturer’s protocol, except that the reaction time was increased to

(3) Recruitment of MLL1 complex is essential for SETBP1 to induce myeloid transformationiScienceJanuary 21, 2022Nguyen N, Gudmundsson KO, Soltis AR, Oakley K, Roy KR, Han Y, Gurnari C, Maciejewski JP, Crouch G, Ernst P, et al.Stranded mRNA Library Prep Kit,Illumina,Cat# 20020594 TnT® Coupled Wheat Germ Extract System,Promega,Cat# L4140 Invitrogen™ SuperScript™ IV First-Strand Synthesis System,Invitrogen,Cat# 18091050 ,, Deposited data,Deposited

(4) FIS1 encodes a GA2-oxidase that regulates fruit firmness in tomatoNature CommunicationsDecember 1, 2020Ren Li et al.Confirmed constructs were used for recombinant protein expression in wheat germ TNT expression systems (L4140, Promega). For the enzyme activity assay, the procedure was adapted from previous studies with slight

(5) Novel Protein-oligonucleotide Conjugation Method Involving a High-affinity Capture HaloTagBIO-PROTOCOLSeptember 20, 2020Junshi YazakiTranslation (TNT) T7 coupled wheat germ extract kit for in vitro expression (Promega, catalog number: L4140), store at -80 °C 18. RNasin RNase Inhibitor (Promega, catalog number: N2511), store at -20 °C 19. Magne

(6) A human ESC-based screen identifies a role for the translated lncRNA LINC00261 in pancreatic endocrine differentiationeLifeAugust 3, 2020Bjoern Gaertner et al.in vitro from 0.5 µg linearized plasmid DNA using the TnT Coupled Wheat Germ Extract system (Promega; Cat# L4140) in the presence of 10 mCi/mL [35S]-methionine (Hartmann Analytic) according to manufacturer’s instructions.

(7) The Barley stripe mosaic virus γb protein promotes viral cell-to-cell movement by enhancing ATPase-mediated assembly of ribonucleoprotein movement complexesPLOS PathogensJuly 30, 2020Zhihao Jiang et al.effects. In vitro translation and Co-IP assay The TnT wheat germ extract system (Promega, Cat. # L4140) together with L-[14C(U)]-Leucine (PerkinElmer, Cat. # NEC279E050UC) was used for in vitro translation

(8) Analysis of Drosophila STING Reveals an Evolutionarily Conserved Antimicrobial FunctionCell ReportsJune 19, 2018Marina Martin, Aoi Hiroyasu, R. Marena Guzman, Steven A. Roberts, Alan G. Goodman,GE Healthcare,17-5248 ENrich Q column,Biorad,780-0001 TNT T7 Coupled Wheat Germ Extract System,Promega,L4140 Pierce high capacity streptavidin agarose resin,ThermoFisher,20357 Luciferase Assay System,Promega,E1500 Renilla Luciferase Assay System

(9) Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5aNature cell biologyNovember 1, 2016Strikoudis A, Lazaris C, Trimarchi T, Galvao Neto AL, Yang Y, Ntziachristos P, Rothbart S, Buckley S, Dolgalev I, Stadtfeld M, et al.Flag/HA-modified pCDNA3.1 vector (Invitrogen) and in vitro translated using TNT Coupled Wheat Germ Extract System (L4140, Promega). 5µg of purified Phf5a protein was added, mixtures were bound overnight at 4°C and HA-tagged

(10) The Arabidopsis Auxin Receptor F-Box Proteins AFB4 and AFB5 Are Required for Response to the Synthetic Auxin PicloramG3 Genes|Genomes|GeneticsMay 1, 2016Michael J. Prigge, Kathleen Greenham, Yi Zhang, Aaron Santner, Cristina Castillejo, Andrew M. Mutka, Ronan C. O’Malley, Joseph R. Ecker, Barbara N. Kunkel, Mark Estelleafb4D215N-4×Myc, and TIR1-Myc were produced from TNT T7 coupled wheat germ extract system (Promega, L4140). Comparable amounts of AFB4-Myc, afb4D215N-Myc, and TIR1-Myc were applied to each pulldown reaction

(11) An interconnected hierarchical model of cell death regulation by the BCL-2 familyNature Cell BiologyOctober 1, 2015Hui-Chen Chen et al.Quick Coupled Transcription/Translation Reticulocyte Lysate or Wheat Germ Extract Systems (L1170 and L4140, Promega) according to the manufacture’s instructions. For the Wheat Germ Extract System, SG5- and pCDNA3-based

(12) An ARID domain-containing protein within nuclear bodies is required for sperm cell formation in Arabidopsis thalianaPLoS geneticsJuly 1, 2014Binglian Zheng, Hui He, Yanhua Zheng, Wenye Wu, Sheila McCormickT7 in vitro transcription/translation with the TNT Coupled Wheat Germ Extract System (Promega, Cat.L4140). 25 ul freshly translated ARID1 protein was added to DNA-bound beads in the IP buffer plus complete

(13) Engineering Gene Networks to Emulate Drosophila Embryonic Pattern FormationPLoS BiologyFebruary 22, 2005Mark Isalan, Caroline Lemerle, Luis Serranoand TNT Coupled Wheat Germ Extract System (Promega, Madison, Wisconsin, United States; #L4130 and #L4140), which comprised 20 μl of TNT wheat germ extract, 1.2 μl of TNT reaction buffer, 0.6 μl of amino acid

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