TNT SP6 High-Yield Wheat Germ Protein Expression System from Promega

Product Specs
ItemTNT SP6 High-Yield Wheat Germ Protein Expression System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberL3261
Quantity10 reactions
Systemwheat germ extract

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Protein homeostasis from diffusion-dependent control of protein synthesis and degradationbioRxivMay 3, 2023Yuping Chen, Jo-Hsi Huang, Connie Phong, James E. FerrellSP6-securin-CFP plasmid in 20 μL H2O with 30 μL SP6 High-Yield Wheat Germ Protein Expression System (TnT® L3261, Promega), and incubating at room temperature for 2 h per the manufacturer’s instructions. CSF extracts

(2) Interactions between TULP3 tubby domain and ARL13B amphipathic helix promote lipidated protein transport to ciliaMolecular biology of the cellMarch 1, 2023Palicharla VR, Hwang SH, Somatilaka BN, Legué E, Shimada IS, Familiari NE, Tran VM, Woodruff JB, Liem KF Jr, Mukhopadhyay S.by in vitro translation using the TNT Sp6 high-yield wheat germ protein expression system (Promega; L3261) from pCS2-Myc-ARL13B vectors generated by gateway cloning. GFP-SNAP-ARL13B was generated by cloning

(3) DNA demethylation affects imprinted gene expression in maize endospermGenome BiologyDecember 1, 2022Qiang Xu, Leiming Wu, Zhixiang Luo, Mei Zhang, Jinsheng Lai, Lin Li, Nathan M. Springer, Qing Licontrol. The ZmO2 and GFP proteins were expressed in vitro using TNT SP6 Wheat Germ Master Mix (Promega L3261) following the manufacturer’s instruction. Separately, DNA from WT and zmros1ab mutant endosperm was

(4) Variation in the fruit development gene POINTED TIP regulates protuberance of tomato fruit tipNature CommunicationsOctober 8, 2022Jianwen Song, Lele Shang, Changxing Li, Wenqian Wang, Xin Wang, Chunli Zhang, Guo Ai, Jie Ye, Changxian Yang, Hanxia Li, et al.PTR-pF3K and PTH-pF3K plasmids were added to the TnT® SP6 High-Yield Wheat Germ Protein Expression System (L3260, Promega, USA) and incubated at 25 °C for 2 h to express the proteins in vitro. EMSAs were performed

(5) Global Profiling of 2-hydroxyisobutyrylome in Common WheatGenomics, Proteomics & BioinformaticsAugust 1, 2022Ning Zhang, Lingran Zhang, Linjie Li, Junyou Geng, Lei Zhao, Yan Ren, Zhongdong Dong, Feng ChenHalo-His-gene was expressed using a 30 μl TNT SP6 High-Yield Wheat Germ Protein Expression System (Catalog No. L3261, Promega). Expression levels were determined by SDS-PAGE and WB. Magne Halo Tag Beads (Catalog No. G728A,

(6) Regulator of G protein signaling 2 inhibits Gαq-dependent uveal melanoma cell growthJournal of Biological ChemistryJune 1, 2022Qian Zhang, Andrew J. Haak, Benita SjögrenIn vitro transcription/translation and co-IP TnT SP6 High-Yield Wheat Germ Protein Expression System kit (L3260; Promega) was used as directed by the manufacturer. The transcription/translation reaction was performed

(7) The Emerging Role of E3 Ubiquitin Ligase SMURF2 in the Regulation of Transcriptional Co-Repressor KAP1 in Untransformed and Cancer Cells and TissuesCancersMarch 22, 2022Shah PA, Boutros-Suleiman S, Emanuelli A, Paolini B, Levy-Cohen G, Blank M.

(8) CEP97 phosphorylation by Dyrk1a is critical for centriole separation during multiciliogenesisThe Journal of cell biologyJanuary 3, 2022Lee M, Nagashima K, Yoon J, Sun J, Wang Z, Carpenter C, Lee HK, Hwang YS, Westlake CJ, Daar IO.and Dvl2 proteins were prepared using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260; Promega) according to the manufacturer’s instructions for the CEP97 protein shift assay. For in vitro

(9) Biofoundry-assisted expression and characterization of plant proteinsSynthetic BiologyOctober 21, 2021Quentin M Dudley, Yao-Min Cai, Kalyani Kallam, Hubert Debreyne, Jose A Carrasco Lopez, Nicola J Patrongerm cell-free expressions used the TNT® SP6 High-Yield Wheat Germ Protein Expression System L3260/L3261 (Promega) according to manufacturers’ instructions. Plasmids were added at a final concentration of

(10) The acyltransferase PMAT1 malonylates brassinolide glucosideJournal of Biological ChemistryFebruary 16, 2021Sufu Gan, Wilfried Rozhon, Elisabeth Varga, Jyotirmoy Halder, Franz Berthiller, Brigitte Poppenbergeraccording to the manufacturer instructions with the TNT SP6 High-Yield Wheat Germ Master Mix (Promega, Cat. L3261). The reactions were incubated at 25 °C for 2 h prior addition of 25 μl glycerol for storage at −20

(11) Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed GerminationInternational Journal of Molecular SciencesFebruary 7, 2021Cheng-Chao Zhu, Chu-Xin Wang, Chen-Ya Lu, Jin-Dong Wang, Yu Zhou, Min Xiong, Chang-Quan Zhang, Qiao-Quan Liu, Qian-Feng Li#G184A). OsbZIP09 fused to HaloTag was expressed using a TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260, Promega, Madison, WI, USA), and was purified using Magne HaloTag Beads (G7281, Promega). The Magne

(12) Bistable, Biphasic Regulation of PP2A-B55 Accounts for the Dynamics of Mitotic Substrate PhosphorylationCurrent BiologyFebruary 1, 2021Julia Kamenz, Lendert Gelens, James E. Ferrellwas in vitro translated using the TNT SP6 high-yield wheat germ protein expression system (Promega, #L3261). Three or 4 μL of in vitro translated securin-CFP was added to 50 or 80 μL extract. The extract was

(13) Genome-wide binding of SEPALLATA3 and AGAMOUS complexes determined by sequential DNA-affinity purification sequencingNucleic Acids ResearchSeptember 25, 2020Xuelei Lai, Arnaud Stigliani, Jérémy Lucas, Véronique Hugouvieux, François Parcy, Chloe Zubietaproduction was carried out using TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega L3260) according to the manufacturer’s instructions. For single protein DAP-seq, i.e. SEP3, 2 ␮g input plasmid

(14) Short-chain fatty acids bind to apoptosis-associated speck-like protein to activate inflammasome complex to prevent Salmonella infectionPLoS biologySeptember 1, 2020Tsugawa H, Kabe Y, Kanai A, Sugiura Y, Hida S, Taniguchi S, Takahashi T, Matsui H, Yasukawa Z, Itou H, et al.were incubated with 50 μL of TNT SP6 HighYield Wheat Germ Protein Expression System (Promega, cat. no. L3260) and fluorescently labeled using FluoroTect GreenLys in vitro Translation Labeling System (Promega

(15) Extensive signal integration by the phytohormone protein networkNatureJuly 9, 2020Melina Altmann et al.different subsets were expressed in cell-free coupled transcription translation wheat-germ lysate (Promega, L3260) using SP6 promoters. Of each protein pair, one partner was expressed as an N-terminal FLAG-fusion protein

(16) ZmIBH1-1 regulates plant architecture in maizeJournal of Experimental BotanyMay 30, 2020Yingying Cao et al.ZmIBH1-1 fused to HaloTag was expressed using a TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260, Promega), and then was purified using Magne HaloTag Beads (G7281, Promega). The Magne HaloTag Beads

(17) Killer cell proteases can target viral immediate-early proteins to control human cytomegalovirus infection in a noncytotoxic mannerPLoS pathogensApril 1, 2020Shan L, Li S, Meeldijk J, Blijenberg B, Hendriks A, van Boxtel KJWM, van den Berg SPH, Groves IJ, Potts M, Svrlanska A, et al.cell-free transcription/translation system in vitro, TnT SP6 High-Yield Protein Expression System (Promega, L3260) was used for all constructs in pGloSensor vector backbone according to the manufacturer’s protocol.

(18) Domain-specific expression of meristematic genes is defined by the LITTLE ZIPPER protein DTM in tomatoCommunications BiologyDecember 1, 2019Qian Xu, Rong Li, Lin Weng, Yuan Sun, Meng Li, Han Xiaosynthesized in 10 μL reaction volumes using the TnT™ SP6 High-Yield Wheat Germ Protein Expression System (# L3261, Promega) following the supplier’s instructions. For the DNA binding assay, 1 μL aliquots of the synthesized

(19) PISTILLATA paralogs in Tarenaya hassleriana have diverged in interaction specificityBMC Plant BiologyDecember 22, 2018Suzanne de Bruijn, Tao Zhao, Jose M. MuiÃ±o, Eric M. Schranz, Gerco C. Angenent, Kerstin Kaufmann

(20) SNAIL1-mediated downregulation of FOXA proteins facilitates the inactivation of transcriptional enhancer elements at key epithelial genes in colorectal cancer cellsPLOS GeneticsNovember 20, 2017Sabine Jägle, Hauke Busch, Vivien Freihen, Sven Beyes, Monika Schrempp, Melanie Boerries, Andreas Hecht

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TNT SP6 High-Yield Wheat Germ Protein Expression System from Promega

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TNT SP6 High-Yield Wheat Germ Protein Expression System from Promega

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