TNT SP6 High-Yield Wheat Germ Protein Expression System from Promega

Product Specs
ItemTNT SP6 High-Yield Wheat Germ Protein Expression System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberL3260
Quantity40 reactions
Systemwheat germ extract

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Molecular mechanism analysis of ZmRL6 positively regulating drought stress tolerance in maizeStress BiologyNovember 16, 2023Pengyu Zhang, Tongchao Wang, Liru Cao, Zhixin Jiao, Lixia Ku, Dandan Dou, Zhixue Liu, Jiaxu Fu, Xiaowen Xie, Yingfang Zhu, et al.ZmRL6 fused to HaloTag was expressed using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260) (Promega), before getting purified by using Magne HaloTag Beads (G7281) (Promega). Next, the ZmRL6-HaloTag

(2) LEAFY homeostasis is regulated via ubiquitin-dependent degradation and sequestration in cytoplasmic condensatesiScienceJune 16, 2023Ulla Dolde, Fernando Muzzopappa, Charlotte Delesalle, Julie Neveu, Fabian Erdel, Grégory VertCul1/Rbx1/Skp1,Ubiquigent,Cat#63-1001-025 TnT SP6 High-Yield Wheat Germ Protein Expression System,Promega,Cat#L3260 Anti-FLAG M2 affinity gel,Sigma,Cat#F1804 3x FLAG peptide,GenScript,Cat#RP10586 GFP-Trap Magnetic

(3) TRIM21-dependent target protein ubiquitination mediates cell-free Trim-AwayCell reportsFebruary 28, 2023Mevissen TET, Prasad AV, Walter JC.Kit,Hampton Research,Cat# HR2–434 TnT® SP6 High-Yield Wheat Germ Protein Expression System,Promega,Cat# L3260 Experimental models: Organisms/strains,, Xenopus laevis (females),Nasco,Cat# LM0053MX Xenopus laevis

(4) Variation in the fruit development gene POINTED TIP regulates protuberance of tomato fruit tipNature CommunicationsOctober 8, 2022Jianwen Song, Lele Shang, Changxing Li, Wenqian Wang, Xin Wang, Chunli Zhang, Guo Ai, Jie Ye, Changxian Yang, Hanxia Li, et al.PTR-pF3K and PTH-pF3K plasmids were added to the TnT® SP6 High-Yield Wheat Germ Protein Expression System (L3260, Promega, USA) and incubated at 25 °C for 2 h to express the proteins in vitro. EMSAs were performed

(5) Regulator of G protein signaling 2 inhibits Gαq-dependent uveal melanoma cell growthJournal of Biological ChemistryJune 1, 2022Qian Zhang, Andrew J. Haak, Benita SjögrenIn vitro transcription/translation and co-IP TnT SP6 High-Yield Wheat Germ Protein Expression System kit (L3260; Promega) was used as directed by the manufacturer. The transcription/translation reaction was performed

(6) The Emerging Role of E3 Ubiquitin Ligase SMURF2 in the Regulation of Transcriptional Co-Repressor KAP1 in Untransformed and Cancer Cells and TissuesCancersMarch 22, 2022Shah PA, Boutros-Suleiman S, Emanuelli A, Paolini B, Levy-Cohen G, Blank M.in vitro binding assay, FLAG-KAP1 (produced using the TNT® SP6 high-yield wheat germ expression system (L3260, Promega, Madison, WI, USA) was co-incubated with GST-SMURF2 (purified from bacteria). The reaction

(7) CEP97 phosphorylation by Dyrk1a is critical for centriole separation during multiciliogenesisThe Journal of cell biologyJanuary 3, 2022Lee M, Nagashima K, Yoon J, Sun J, Wang Z, Carpenter C, Lee HK, Hwang YS, Westlake CJ, Daar IO.and Dvl2 proteins were prepared using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260; Promega) according to the manufacturer’s instructions for the CEP97 protein shift assay. For in vitro

(8) Biofoundry-assisted expression and characterization of plant proteinsSynthetic BiologyOctober 21, 2021Quentin M Dudley, Yao-Min Cai, Kalyani Kallam, Hubert Debreyne, Jose A Carrasco Lopez, Nicola J Patronwheat germ cell-free expressions used the TNT® SP6 High-Yield Wheat Germ Protein Expression System L3260/L3261 (Promega) according to manufacturers’ instructions. Plasmids were added at a final concentration

(9) ZmCCT regulates photoperiod-dependent flowering and response to stresses in maizeBMC Plant BiologyOctober 6, 2021Huihui Su, Jiachen Liang, Salah Fatouh Abou-Elwafa, Haiyang Cheng, Dandan Dou, Zhenzhen Ren, Jiarong Xie, Zhihui Chen, Fengran Gao, Lixia Ku, et al.employed to fuse the ZmCCT into the HaloTag. The TNT SP6 High-Yield Wheat Germ Protein Expression System (L3260, Promega) was then implemented to express the ZmCCT-HaloTag fusion protein. Magen HaloTag Beads (G7281,

(10) The acyltransferase PMAT1 malonylates brassinolide glucosideJournal of Biological ChemistryFebruary 16, 2021Sufu Gan, Wilfried Rozhon, Elisabeth Varga, Jyotirmoy Halder, Franz Berthiller, Brigitte Poppenberger

(11) Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed GerminationInternational Journal of Molecular SciencesFebruary 7, 2021Cheng-Chao Zhu, Chu-Xin Wang, Chen-Ya Lu, Jin-Dong Wang, Yu Zhou, Min Xiong, Chang-Quan Zhang, Qiao-Quan Liu, Qian-Feng Li#G184A). OsbZIP09 fused to HaloTag was expressed using a TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260, Promega, Madison, WI, USA), and was purified using Magne HaloTag Beads (G7281, Promega). The Magne

(12) Bistable, Biphasic Regulation of PP2A-B55 Accounts for the Dynamics of Mitotic Substrate PhosphorylationCurrent BiologyFebruary 1, 2021Julia Kamenz, Lendert Gelens, James E. Ferrellwas in vitro translated using the TNT SP6 high-yield wheat germ protein expression system (Promega, #L3261). Three or 4 μL of in vitro translated securin-CFP was added to 50 or 80 μL extract. The extract was

(13) Genome-wide binding of SEPALLATA3 and AGAMOUS complexes determined by sequential DNA-affinity purification sequencingNucleic Acids ResearchSeptember 25, 2020Xuelei Lai, Arnaud Stigliani, Jérémy Lucas, Véronique Hugouvieux, François Parcy, Chloe Zubietaproduction was carried out using TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega L3260) according to the manufacturer's instructions. For single protein DAP-seq, i.e. SEP3, 2 μg input plasmid

(14) Short-chain fatty acids bind to apoptosis-associated speck-like protein to activate inflammasome complex to prevent Salmonella infectionPLoS biologySeptember 1, 2020Tsugawa H, Kabe Y, Kanai A, Sugiura Y, Hida S, Taniguchi S, Takahashi T, Matsui H, Yasukawa Z, Itou H, et al.incubated with 50 μL of TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega, cat. no. L3260) and fluorescently labeled using FluoroTect GreenLys in vitro Translation Labeling System (Promega,

(15) Extensive signal integration by the phytohormone protein networkNatureJuly 9, 2020Melina Altmann et al.different subsets were expressed in cell-free coupled transcription translation wheat-germ lysate (Promega, L3260) using SP6 promoters. Of each protein pair, one partner was expressed as an N-terminal FLAG-fusion protein,

(16) ZmIBH1-1 regulates plant architecture in maizeJournal of Experimental BotanyMay 30, 2020Yingying Cao et al.ZmIBH1-1 fused to HaloTag was expressed using a TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260, Promega), and then was purified using Magne HaloTag Beads (G7281, Promega). The Magne HaloTag Beads

(17) Killer cell proteases can target viral immediate-early proteins to control human cytomegalovirus infection in a noncytotoxic mannerPLoS pathogensApril 1, 2020Shan L, Li S, Meeldijk J, Blijenberg B, Hendriks A, van Boxtel KJWM, van den Berg SPH, Groves IJ, Potts M, Svrlanska A, et al.cell-free transcription/translation system in vitro, TnT SP6 High-Yield Protein Expression System (Promega, L3260) was used for all constructs in pGloSensor vector backbone according to the manufacturer’s protocol.

(18) Domain-specific expression of meristematic genes is defined by the LITTLE ZIPPER protein DTM in tomatoCommunications BiologyDecember 1, 2019Qian Xu, Rong Li, Lin Weng, Yuan Sun, Meng Li, Han Xiaosynthesized in 10 μL reaction volumes using the TnT™ SP6 High-Yield Wheat Germ Protein Expression System (# L3261, Promega) following the supplier’s instructions. For the DNA binding assay, 1 μL aliquots of the synthesized

(19) Multi-level Modulation of Light Signaling by GIGANTEA Regulates Both the Output and Pace of the Circadian ClockDevelopmental CellJune 1, 2019Maria A. Nohales, Wanlu Liu, Tomas Duffy, Kazunari Nozue, Mariko Sawa, Jose L. Pruneda-Paz, Julin N. Maloof, Steven E. Jacobsen, Steve A. KaySystem,Invitrogen,Cat#PQ1000101 TnT® SP6 High-Yield Wheat Germ Protein Expression System,Promega,Cat#L3260 DC Protein Assay,BioRad,Cat#5000116 "Supersignal West Pico, Dura, and Femto substrates",Pierce,"Cat#34577,

(20) A Comprehensive Drosophila melanogaster Transcription Factor InteractomeCell ReportsApril 16, 2019Leila Shokri et al.1-Step Human Coupled IVT Kit - DNA,ThermoFisher SCIENTIFIC,88881 TnT® Coupled Wheat Germ Extract System,Promega,L3260 Deposited Data,, PBM data and derived DNA binding specificity motifs,This paper,http://the_brain.bwh.harvard.edu/uniprobe/

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7/25/2023Coupled Transcription/Translation Kit From Promega Ganesh PusapatiHedgehog signaling plays a critical role in embryonic development. It also controls adult tissue homeostasis and deregulation of this pathway results in basal cell carcinoma and medulloblastoma. A ... Read Review

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TNT SP6 High-Yield Wheat Germ Protein Expression System from Promega

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TNT SP6 High-Yield Wheat Germ Protein Expression System from Promega

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