ClonePix 2 Cell Selection System from Molecular Devices LLC

Product Specs
ItemClonePix 2 Cell Selection System
CompanyMolecular Devices LLC
Price Get Pricing Get Quote for this Product
Catalog NumberClonePix 2
ApplicationsAntibody development for biotherapeutics / Antibody discovery for research / Protein expression and production / Stem cell screening/Maintenance of stem cell lines
LightingWhite light Trans-illumination / White light Epi-illumination / Fluorescence
CameraIntegrated 16-bit cooled CCD camera
SoftwareDedicated imaging software pre-installed on a high specification PC
Plate FormatsPetriWell-6 plate / PetriWell-1 plate / Greiner 6 well plate / Nunc 6 well plate / Nunc OmniTray
Dimensions(WxDxH) 101.0 x 90.0 x 149.0 cm (39.7 x 35.4 x 58.6 inches)

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Molecular Devices LLC

3860 N First Street

San Jose, California 95134

United States

Phone: 408-747-1700

Company Profile

Website: http://www.moleculardevices.com/

(1) TMEM106B is a receptor mediating ACE2-independent SARS-CoV-2 cell entryCellAugust 3, 2023Jim Baggen, Maarten Jacquemyn, Leentje Persoons, Els Vanstreels, Valerie E. Pye, Antoni G. Wrobel, Valeria Calvaresi, Stephen R. Martin, Chloë Roustan, Nora B. Cronin, et al.hybridomas were isolated using the Clonacell-HY Hybridoma Kit (Stemcell Technologies, Vancouver, BC) and Clonepix2 colony picker (Molecular Devices, Sunnyvale, CA), and supernatants were screened by FACS on HEK293 cells

(2) Validation of immunoassays for the Chlamydia trachomatis antigen Pgp3 using a chimeric monoclonal antibodyScientific ReportsMay 4, 2023Brook Goodhew, Xiaoling Tang, Jason Goldstein, Joo Lee, Diana Martin, Sarah GwynSwitzerland). After ten days of plating, clones grown in the presence of CloneDetect were screened by ClonePix 2 system (Molecular Devices, San Jose, California, USA) per the manufacturer’s instructions. A total of

(3) Development of HEK-293 Cell Lines Constitutively Expressing Flaviviral Antigens for Use in DiagnosticsMicrobiology SpectrumJune 29, 2022Jordan A. Powers, Benjamin Skinner, Brent S. Davis, Brad J. Biggerstaff, Lucy Robb, Elizabeth Gordon, William M. de Souza, Marcilio Jorge Fumagalli, Amanda E. Calvert, Gwong-Jen Changwere cultured for 7 to 14 days at 37°C with 5% CO2. Colonies were identified and selected using the ClonePix2 automated mammalian colony picker (Molecular Devices, CA, USA) according to the manufacturer’s instructions.

(4) Monoclonal antibodies to Cache Valley virus for serological diagnosisPLOS Neglected Tropical DiseasesJanuary 24, 2022Benjamin Skinner, Sierra Mikula, Brent S. Davis, Jordan A. Powers, Holly R. Hughes, Amanda E. Calvertfor 10 days at 37°C in 5% CO2. Clones expressing anti-mouse IgG were identified and picked with the ClonePix2 automated colony picker (Molecular Devices, Sunnyvale, CA, USA) according to the manufacturer’s protocol.

(5) ZRC3308 Monoclonal Antibody Cocktail Shows Protective Efficacy in Syrian Hamsters against SARS-CoV-2 InfectionVirusesDecember 3, 2021Yadav PD, Mendiratta SK, Mohandas S, Singh AK, Abraham P, Shete A, Bandyopadhyay S, Kumar S, Parikh A, Kalita P, et al.IgG H + L specific, fluorescein (Molecular Devices, San Jose, CA, USA), for clonal selection using ClonePix2 (Molecular Devices, San Jose, CA, USA). After 7–10 days, based on high exterior mean fluorescent intensity,

(6) Therapy for Argentine hemorrhagic fever in nonhuman primates with a humanized monoclonal antibodyProceedings of the National Academy of Sciences of the United States of AmericaMarch 16, 2021Zeitlin L, Cross RW, Geisbert JB, Borisevich V, Agans KN, Prasad AN, Enterlein S, Aman MJ, Bornholdt ZA, Brennan MB, et al.heavy and light chains. Stable pools were selected with methionine sulfoximine and enriched using a ClonePix2 colony picker (Molecular Devices). ClonePix nonclonal isolates were grown and expanded in static culture,

(7) Rich production media as a platform for CHO cell line developmentAMB ExpressDecember 1, 2020Yong Jae Kim, Sang Kyul Han, Seongtae Yoon, Chan Wha Kimconditions for 48 h in the presence of HT. After 48 h, cells were either used for mini-pool assessment or ClonePix2 (Molecular Devices, San Jose, CA, USA) experiments. The cells were further cultured without HT and adding

(8) A kinase-deficient NTRK2 splice variant predominates in glioma and amplifies several oncogenic signaling pathwaysNature communicationsJune 12, 2020Pattwell SS, Arora S, Cimino PJ, Ozawa T, Szulzewsky F, Hoellerbauer P, Bonifert T, Hoffstrom BG, Boiani NE, Bolouri H, et al.Harvard Apparatus). Hybridomas secreting peptide specific antibody were identified and isolated using a ClonePix2 (Molecular Devices, CPII) colony picker. Antibodies from the picked clones were validated for peptide

(9) Transferrin Receptor Is a Specific Ferroptosis MarkerCell reportsMarch 10, 2020Feng H, Schorpp K, Jin J, Yozwiak CE, Hoffstrom BG, Decker AM, Rajbhandari P, Stokes ME, Bender HG, Csuka JM, et al.Approximately 4,750 hybridomas positive for IgG secretion were then identified and isolated using a ClonePix2 colony picker (Molecular Devices, CPII). Primary screening of the 4,750 clones was performed by indirect

(10) Multiplexed Competitive Screening of One-Bead-One-Component Combinatorial Libraries Using a ClonePix 2 Colony SorterInternational Journal of Molecular SciencesOctober 16, 2019R. Ashton Lavoie, Alice di Fazio, Ruben G. Carbonell, Stefano Menegattipicking to be manually operated. To expand on this “cast-image-pick” approach, this work adapted a ClonePix 2 colony picker (Molecular Devices, Sunnyvale, CA) to fully automate the bead picking process with minimal

(11) New Monoclonal Antibodies for a Selective Detection of Membrane-Associated and Soluble Forms of Carbonic Anhydrase IX in Human Cell Lines and Biological SamplesBiomoleculesJuly 25, 2019Dovile Stravinskiene, Aiste Imbrasaite, Vilma Petrikaite, Daumantas Matulis, Jurgita Matuliene, Aurelija Zvirblienedays without moving to prevent cell scattering. Before procedure for hybridoma clones isolation, the ClonePix 2 system (Molecular Devices) was sanitized and calibrate according to manufacturer’s recommendations.

(12) Targeted Capture of Chinese Hamster Ovary Host Cell Proteins: Peptide Ligand DiscoveryInternational Journal of Molecular SciencesApril 8, 2019R. Ashton Lavoie, Alice di Fazio, R. Kevin Blackburn, Michael B. Goshe, Ruben G. Carbonell, Stefano MenegattiLyophilized polyclonal human IgG was obtained from Athens Research (Athens, GA, USA). CloneMatrix for ClonePix 2 screening was generously provided by Molecular Devices (Sunnyvale, CA, USA). The model mAb production

(13) Production of a recombinant monoclonal antibody to Herpes Simplex Virus glycoprotein D for immunoaffinity purification of tagged proteinsJournal of Immunological MethodsFebruary 1, 2019Sara M. O'Rourke, Bin Yu, Javier F. Morales, Chelsea M. Didinger, David L. Alexander, Christopher Vollmers, Phillip W. Bermantranslation. 3.2. Stable CHO-S r34.1 clone selection Stable G418 resistant CHO-S lines were generated using a ClonePix2 (Molecular devices Sunnyvale, CA) robot, following electroporation of CHO-S cells with linearized vector

(14) Secretion analysis of intracellular “difficult-to-express” immunoglobulin G (IgG) in Chinese hamster ovary (CHO) cellsCytotechnologyFebruary 1, 2019Kohei Kaneyoshi, Kouki Kuroda, Keiji Uchiyama, Masayoshi Onitsuka, Noriko Yamano-Adachi, Yuichi Koga, Takeshi OmasaDNA Transfection 307 Reagent (Roche, Mannheim, Germany). A single clone was selected from the constructed cell pool by ClonePix 2 System (Molecular Devices, San Jose, CA, USA) and adapted to serum-free medium. The resulting established

(15) A Two-Antibody Pan-Ebolavirus Cocktail Confers Broad Therapeutic Protection in Ferrets and Nonhuman PrimatesCell host & microbeJanuary 9, 2019Bornholdt ZA, Herbert AS, Mire CE, He S, Cross RW, Wec AZ, Abelson DM, Geisbert JB, James RM, Rahim MN, et al.for further development. Enriched stable pools were created from the original transfected pool using ClonePix2 technology (Molecular Devices). A vial from an enriched pool of CHOK1-AF cells expressing ADI-23774Af

(16) Development of a Stable MGAT1− CHO Cell Line to Produce Clade C gp120 With Improved Binding to Broadly Neutralizing AntibodiesFrontiers in ImmunologyOctober 5, 2018Rachel C. Doran, Bin Yu, Meredith Wright, Sara M. O'Rourke, Lu Yin, Jennie M. Richardson, Gabriel Byrne, Kathryn A. Mesa, Phillip W. Bermanexpressing rgp120 were selected on basis of fluorescence and default selection parameters using the ClonePix2 (Molecular Devices, Sunnyvale, CA). Cell culture in shake flasks was performed in BalanCD® CHO Growth

(17) Robotic selection for the rapid development of stable CHO cell lines for HIV vaccine productionPLOS ONEAugust 2, 2018Sara M. O’Rourke, Gabriel Byrne, Gwen Tatsuno, Meredith Wright, Bin Yu, Kathryn A. Mesa, Rachel C. Doran, David Alexander, Phillip W. Bermanelectroporation device (MaxCyte STX, MaxCyte Inc., Gaithersburg, MD), a robotic cell selection system, ClonePix2 (Molecular Devices, Sunnyvale, CA) and a novel cell line (MGAT1- CHO 3.4F10) that was recently developed

(18) A natural human monoclonal antibody targeting Staphylococcus Protein A protects against Staphylococcus aureus bacteremiaPloS oneJanuary 1, 2018Varshney AK, Kuzmicheva GA, Lin J, Sunley KM, Bowling RA Jr, Kwan TY, Mays HR, Rambhadran A, Zhang Y, Martin RL, et al.plates (Greiner Bio-One, 657185) in a 37°C incubator with 10% CO2. Top producing clones were picked by ClonePix2 (Molecular Devices) according to their high FITC 500 ms Sum Total Intensities on Day 14. Selected clones

(19) Design, development and characterization of ACT017, a humanized Fab that blocks platelet's glycoprotein VI function without causing bleeding risksmAbsAugust 18, 2017Kristell Lebozec, Martine Jandrot-Perrus, Gilles Avenard, Olivier Favre-Bulle, Philippe Billialdcells were prepared at Catalent after multiple rounds of transduction. Cells were subcloned using the ClonePix˜2 system (Molecular Devices). The most viable clones were assayed by ELISA for GPVI-Fc binding and antibody

(20) Increased antibody productivity in Chinese Hamster Ovary cells through induction of chromosomal instability by cell fusionBMC ProceedingsDecember 14, 2015YuanShan Lai, Noriko Yamano, Masayoshi Onitsuka, Takeshi OmasaFive cell lines were selected from untreated and PEG-treated cells by single cell cloning using the ClonePix2 system (Molecular Devices, Sunnyvale, CA, USA) represented as clones NON-HYB-1-NON-HYB-5 and clones

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