NEB 5-alpha FIqCompetentE. coli(High Efficiency) from New England Biolabs

Product Specs
ItemNEB 5-alpha FIqCompetentE. coli(High Efficiency)
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberC2992I
Quantity6 x 0.2 ml
GenotypeF' proA⁺B⁺ lacI^q Δ(lacZ)M15 zzf::Tn10 (Tet^R)/fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
Cell Type (standardized)Competent Cells
Validated Species ReactivityEscherichia
SpeciesE. coli
Temperature-80 C
Cell TypeCompetent Cells

Specifications/Features

Transformation Efficiency:
1-3 x 10⁹ cfu/µg

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Generation of porcine PK-15 cells lacking the Ifnar1 or Stat2 gene to optimize the efficiency of viral isolationPLOS ONENovember 8, 2023Maya Shofa, Akatsuki SaitoZ9633N). The plasmid was amplified using NEB 5-alpha F′ Iq Competent E. coli (High Efficiency) (NEB, Cat# C2992H) and extracted using the PureYield Plasmid Miniprep System (Promega, Madison, WI, USA, Cat# A1222).

(2) The minimal intrinsic stochasticity of constitutively expressed eukaryotic genes is sub-PoissonianSci AdvAugust 9, 2023Douglas E. Weidemann, James Holehouse, Abhyudai Singh, Ramon Grima, Silke Haufvector using Gibson assembly (NEB, E2611) and transformed into NEB 5-alpha Competent E. coli (NEB, C2992). Vectors were sequenced by Sanger sequencing. Sequences were discarded if the UTR appeared to begin

(3) Potent Omicron-neutralizing antibodies isolated from a patient vaccinated 6 months before Omicron emergenceCell ReportsMay 30, 2023Kathryn M. Hastie, Xiaoying Yu, Fernanda Ana-Sosa-Batiz, Dawid S. Zyla, Stephanie S. Harkins, Chitra Hariharan, Hal Wasserman, Michelle A. Zandonatti, Robyn Miller, Erin Maule, et al.14001-013 NEBuilder HiFi DNA Assembly master mix,NEB,Cat# E2621L NEB 5 alpha F'Iq competent E coli,NEB,Cat# C2992I E-gel96 2% with SYBR safe,Invitrogen,Cat# G720802 Qiaprep 96 Turbo Miniprep kit,Qiagen,Cat# 27191 "SARS-CoV-2

(4) Targeting NETs using dual-active DNase1 variantsFrontiers in ImmunologyMay 23, 2023Hanna Englert, Josephine Göbel, Danika Khong, Maryam Omidi, Nina Wolska, Sandra Konrath, Maike Frye, Reiner K. Mailer, Manu Beerens, Julian C. Gerwers, et al.For amplification, plasmids were transformed in NEB 5-alpha F´Iq competent E.coli (high efficiency, C2992H, New England Biolabs) and isolated from bacterial cells using PureLink HiPure Plasmid Maxiprep Kit (K210007,

(5) An actin remodeling role for Arabidopsis processing bodies revealed by their proximity interactomeThe EMBO JournalMay 2, 2023Chen Liu, Andriani Mentzelopoulou, Amna Muhammad, Andriy Volkov, Dolf Weijers, Emilio Gutierrez‐Beltran, Panagiotis N MoschouE. coli,New England BioLabs,C3019I NEB® 5‐alpha F'Iq Competent E. coli (High Efficiency),New England BioLabs,C2992I Agrobacterium tumefaciens GV3101,N/A,N/A Arabidopsis thaliana Columbia,35Spro:sGFP‐TurboID‐HF/WT,Arora

(6) Dual function of Rab1A in secretion and autophagy: hypervariable domain dependenceLife science allianceMay 1, 2023Gyurkovska V, Murtazina R, Zhao SF, Shikano S, Okamoto Y, Segev N.pEGFP-C1-Fwd. The two PCR products were then co-transformed into NEB 5-α F’Iq Competent Escherichia coli (C2992I; New England BioLabs) and recombinant plasmids resulting from in vivo homologous recombination were

(7) The minimal intrinsic stochasticity of constitutively expressed eukaryotic genes is sub-PoissonianbioRxivMarch 17, 2023Douglas E. Weidemann, Abhyudai Singh, Ramon Grima, Silke Haufvector using Gibson assembly (NEB, E2611) and transformed into NEB 5-alpha competent E. coli (NEB, C2992). Vectors were sequenced by Sanger sequencing. Sequences were discarded if the UTR appeared to begin

(8) Efficient modification and preparation of circular DNA for expression in cell cultureCommunications BiologyDecember 21, 2022Roman Teo Oliynyk, George M. ChurchE-GELS EX,Invitrogen Corporation,Cat#G402021 NEB 5-alpha F̀Iq Competent E. coli,New England BioLabs,Cat#C2992H HEK293T,ATCC,Cat#CRL-3216 pCMV-PEmax-P2A-MLH1dn,Addgene,Cat#174828 Reagents and resources.

(9) Transcription factor paralogs orchestrate alternative gene regulatory networks by context-dependent cooperation with multiple cofactorsNature CommunicationsDecember 1, 2022Siqian Feng, Chaitanya Rastogi, Ryan Loker, William J. Glassford, H. Tomas Rube, Harmen J. Bussemaker, Richard S. Manncells to express high levels of the lacI protein, and were performed using 5-alpha F’ Iq cells (NEB C2992H). The EGFP fragment was amplified using primers EGFP-5’ and EGFP-3’, and the TEV-MCS fragment, which

(10) Engineering receptors in the secretory pathway for orthogonal signalling controlNature CommunicationsNovember 29, 2022Mohamed Mahameed, Pengli Wang, Shuai Xue, Martin Fusseneggertransformed and propagated in XL10-Gold® ultra-competent Escherichia coli (New England BioLabs, cat. no. C2992) and extracted using a plasmid mini-prep kit (Zymo Research, Irvine, CA, USA, cat. no. D4054) or a ZymoPURE

(11) Generation of a bovine cell line for gene engineering using an HIV-1-based lentiviral vectorScientific ReportsOctober 18, 2022Nanami Morizako, Erika P. Butlertanaka, Yuri L. Tanaka, Honoka Shibata, Tamaki Okabayashi, Hirohisa Mekata, Akatsuki SaitoThen, the plasmid was amplified using NEB 5-alpha F′Iq Competent E. coli (High Efficiency) (NEB, Cat# C2992H). The plasmids were extracted with PureYield Plasmid Miniprep System (Promega, Cat# A1222). The sequences

(12) EMT alterations in the solute carrier landscape uncover SLC22A10/A15 imposed vulnerabilities in pancreatic canceriScienceMay 20, 2022Nayak D, Weadick B, Persaud AK, Raj R, Shakya R, Li J, Campbell MJ, Govindarajan R.,Thermo Fisher Scientific,Cat# C404003 NEB 5-alpha F’ Iq competent E. coli cells,New England Biolabs,Cat# C2992I NEB Stable competent E. coli cells,New England Biolabs,Cat# C3040I GIPZ Lentiviral Empty Vector shRNA

(13) A bacterial three-hybrid assay for forward and reverse genetic analysis of RNA–protein interactionsNature ProtocolsApril 1, 2022Oliver M. Stockert, Chandra M. Gravel, Katherine E. Berrycloning strain that provides lacIq, e.g. NEB 5-α F’Iq cells (New England Biolabs, NEB.com, cat. no. C2992I). For B3H assay, use an E. coli reporter strain from Table 3. Store competent cells at −80°C for up

(14) Engineering a Rapid Insulin Release System Controlled By Oral Drug AdministrationAdvanced ScienceMarch 1, 2022Mohamed Mahameed, Shuai Xue, Bozhidar‐Adrian Stefanov, Ghislaine Charpin‐El Hamri, Martin Fusseneggertransfected and propagated in XL10‐Gold ultracompetent Escherichia coli (New England Biolabs, cat. no. C2992) and DNA was extracted using a plasmid mini‐prep kit (Zymo Research, Irvine, CA, USA, cat. no. D4054)

(15) SARS-CoV-2 mRNA vaccine induces robust specific and cross-reactive IgG and unequal neutralizing antibodies in naive and previously infected peopleCell reportsFebruary 1, 2022Narowski TM, Raphel K, Adams LE, Huang J, Vielot NA, Jadi R, de Silva AM, Baric RS, Lafleur JE, Premkumar L.strains,Bacterial and virus strains,Bacterial and virus strains ,, E.coli NEB 5-alpha F'Iq,New England Biolabs,C2992H E.coli BL21(DE3)pLysS,Premkumar Lab,N/A SARS-CoV-2 luciferase reporter virus,Baric lab,N/A ,, Biological

(16) Allosteric regulation within the highly interconnected structural scaffold of AraC/XylS homologs tolerates a wide range of amino acid changesProteins: Structure, Function, and BioinformaticsJanuary 1, 2022Hunter R. Picard, Kristen S. Schwingen, Lisa M. Green, David L. Shis, Susan M. Egan, Matthew R. Bennett, Liskin Swint‐KruseF’Iq” cells (High Efficiency 5-alpha F’Iq Competent; New England Biolabs Ipswitch, MA, USA; product #C2992I; [F´ proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10 (TetR) / fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80Δ(lacZ)M15 gyrA96

(17) Unraveling the Structural Basis of Selective Inhibition of Human Cytochrome P450 3A5Journal of the American Chemical SocietyNovember 10, 2021Jingheng Wang, Cameron D. Buchman, Jayaraman Seetharaman, Darcie J. Miller, Andrew D. Huber, Jing Wu, Sergio C. Chai, Efren Garcia-Maldonado, William C. Wright, Jude Chenge, et al.previously published method.30 E. coli DH5α_F′_Iq cells (New England Biolabs, Ipswich, MA, catalog no. C2992H) were cotransformed with the expression plasmid and a pGro7 plasmid (Takara Bio USA, Mountain View,

(18) Genetically Encoded Protein Thermometer Enables Precise Electrothermal Control of Transgene ExpressionAdvanced ScienceNovember 1, 2021Bozhidar‐Adrian Stefanov, Ana P. Teixeira, Maysam Mansouri, Adrian Bertschi, Krzysztof Krawczyk, Ghislaine Charpin‐El Hamri, Shuai Xue, Martin FusseneggerThe plasmids were amplified in XL10 Gold chemically competent cells (New England Biolabs, cat. no. C2992) and DNA was extracted using a plasmid miniprep kit (Zymo Research, Irvine, California, USA, cat. no.

(19) Engineered yeast tolerance enables efficient production from toxified lignocellulosic feedstocksScience AdvancesJune 25, 2021Felix H. Lam, Burcu Turanlı-Yıldız, Dany Liu, Michael G. Resch, Gerald R. Fink, Gregory Stephanopouloshour to overnight at room temperature. Chemically competent Escherichia coli (New England Biolabs, #C2992) were transformed and cultured per the manufacturer’s instructions, and ampicillin-resistant isolates

(20) Tau seeds are subject to aberrant modifications resulting in distinct signaturesCell ReportsApril 27, 2021Jui-Heng Tseng et al.E. coli,New England Biolabs,Cat#C3040I NEB® 5-alpha F’Iq Competent E. coli,New England Biolabs,Cat#C2992I NEB® 5-alpha Competent E. coli,New England Biolabs,Cat#C2988J ,, "Chemicals, peptides, and recombinant

Be the First to Write a Review!