Description
The B16-F1 cell line, derived from mouse melanoma, is characterized by a fibroblast-like morphology, comprising a mixture of spindle-shaped and epithelial-like cells, B16-F1 cells offer a unique platform for studying melanin-related functions and pathways. This capacity to produce melanin is particularly noteworthy, as it enables researchers to delve into melanins diverse functions within these cells. B16-F1 cells adhere to a substrate for growth and proliferation and thrive in a nutrient-rich medium, typically composed of DMEM supplemented with 2mM Glutamine and 10% Fetal Bovine Serum (FBS), ensuring their optimal survival and growth. Moreover, these cells have found extensive application in various research domains. For example, B16-F1 cells have been instrumental in the investigation of the cytotoxic and antioxidant properties of supercritical CO2 extract from Usnea barbata and the assessment of the antiproliferative activity of essential oils extracted from Achillea ligustica. Such studies underscore the versatility of B16-F1 cells for assessing the therapeutic potential of natural compounds and their impact on cellular processes. Furthermore, B16-F1 cells have been found to have low metastatic potential, making them suitable for investigations related to cancer metastasis. Their stable metastatic properties, make them an ideal model for evaluating the efficacy of anti-cancer immunotherapies and investigating tumour progression. Their stable metastatic properties, even after multiple subculture passages, render them ideal for evaluating the efficacy of anti-cancer immunotherapies and investigating tumor progression. With a doubling time of approximately 22 hours, these cells are well-suited for various research applications, including 3D cell culture, high-throughput screening, and toxicology studies