Description
The murine cell line C127I, derived from malignant neoplasms of the mammary gland, exhibits a distinctive epithelial nature, rendering it a valuable asset for a variety of applications in research. Notably, C127I cells have undergone genetic modification through the introduction of a chimeric plasmid comprising bovine papillomavirus and the human interferon (HuIFN) gene, particularly IFN-gamma or IFN-alpha 5, regulated by the Simian virus 40 early promoters. This genetic transformation results in the maintenance of 30-50 extrachromosomal copies of the hybrid plasmid and the continuous secretion of elevated levels of HuIFNs. As a result, Mouse C127I Cells present an exciting opportunity for researchers to investigate the expression and functions of diverse proteins and their implications on biological systems, offering a valuable model system for studying mammary biology and related diseases. For example, C127I cells serve as a versatile platform for various applications, including acting as a transfection host for the transformation with bovine papillomavirus DNA plasmids, facilitating the visualization of sarcoma virus-induced foci, and enabling quantitative in vitro assays for bovine papilloma virus research, further enhancing their utility in scientific investigations