Human adenovirus 5 from ATCC

Product Specs
ItemHuman adenovirus 5
CompanyATCC
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberVR-1516
Quantity1
Typevirus
SpeciesAdenoviridae, Mastadenovirus, Human adenovirus C
Cell Type (standardized)Microbial Cells
ApplicationsAssay development, Cell and gene therapy (CGT) development Cell and gene therapy (CGT) development
Breed / StrainAdenovirus Type 5 Reference Material
Research AreaAssay development, Cell and gene therapy (CGT) development

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) Improvement of precision in recombinant Adeno-Associated Virus infectious titer assay with droplet digital PCR as an end-point measurementHuman Gene TherapyAugust 1, 2023Tam Minh Duong, James McAllister, Khalid Eldahan, Jennifer Wang, Eric Onishi, Kate Shen, Robert Schrock, Bingnan Gu, Peng Wangpurchased from ATCC (Cat. No. VR-1616).22,23 Human adenovirus 5 (Ad5) was obtained from ATCC (Cat. No. VR-1516). Upon receipt, AAV2 and Ad5 were thawed at room temperature, aliquoted into 10 μL single-use aliquots,

(2) Antimicrobial Properties of Chitosan-Modified Cotton Fabric Treated with Aldehydes and Zinc Oxide ParticlesMaterials (Basel)July 19, 2023Desislava Staneva, Daniela Atanasova, Daniela Angelova, Petar Grozdanov, Ivanka Nikolova, Ivo Grabchevmeasuring 1 cm2 were cut and immersed into a viral suspension (200 µL) containing HAdV5 (106.2 CCID50, VR-1516™ ATCC, Manassas, VA, USA) and HRSV-S2 (105.5 CCID50, A2 (105.5 CCID50, VR-1540™ ATCC, Manassas, VA,

(3) Complement Activation by Adeno-Associated Virus-Neutralizing Antibody ComplexesHuman Gene TherapyJune 1, 2023Cara West, Joel D. Federspiel, Kara Rogers, Arpana Khatri, Sheila Rao-Dayton, Mireia Fernandez Fernandez Ocana, Sean Lim, Aaron Michael D'Antona, Sandra Casinghino, Suryanarayan Somanathan18–24 h, wild-type human adenovirus 5 was added at a multiplicity of infection (MOI) of 100 (Cat. No. VR-1516; ATCC) for 2 h. Media were aspirated and replaced by vector–serum complexes at an MOI of 1 × 104 vg/cell

(4) Preclinical evaluation of safety and immunogenicity of a primary series intranasal COVID-19 vaccine candidate (BBV154) and humoral immunogenicity evaluation of a heterologous prime-boost strategy with COVAXIN (BBV152)Frontiers in ImmunologyDecember 8, 2022Raju Sunagar, Sai D. Prasad, Raches Ella, Krishna Mohan VadrevuUSA (16). The adenovirus reference material; wild-type human Adenovirus type 5 (Ad5) (Catalogue No: VR1516) was procured from ATCC, USA. The HEK cells were obtained from Microbix, Canada and were propagated

(5) Cytokine-Coding Oncolytic Adenovirus TILT-123 Is Safe, Selective, and Effective as a Single Agent and in Combination with Immune Checkpoint Inhibitor Anti-PD-1CellsJanuary 27, 2021Riikka Havunen, Riikka Kalliokoski, Mikko Siurala, Suvi Sorsa, João M. Santos, Victor Cervera-Carrascon, Marjukka Anttila, Akseli Hemminki(Millipore). The cells were plated on 24-well plates and infected with TILT-123, wild type Adenovirus 5 (VR-1516, ATCC), Ad5/3 replicative control virus (a similar selection device as TILT-123 but no cytokine payload),

(6) Suprachoroidal and Subretinal Injections of AAV Using Transscleral Microneedles for Retinal Gene Delivery in Nonhuman PrimatesMolecular Therapy - Methods & Clinical DevelopmentMarch 13, 2020Glenn Yiu, Sook Hyun Chung, Iris N. Mollhoff, Uyen Tu Nguyen, Sara M. Thomasy, Jesse Yoo, Donna Taraborelli, Glenn Noronhaserum (FBS) at 37°C with 5% CO2. The following day, cells were exposed to human adenovirus type 5 (VR-1516, ATCC) at an MOI of 90 for 2 h to increase susceptibility to AAV8. Aqueous or macaque sera were serially

(7) Comparison of Three Adenovirus Quantitative PCR Assays with ATCC Reference Strains and Clinical SamplesJournal of Clinical MicrobiologyNovember 1, 2019Kimberly Starr, Alexander L. Greninger, Negar Makhsous, Keith R. Jerome, Linda Cookadenovirus 5 type C was purchased from American Type Culture Collection (ATCC; Manassas, VA; catalog no. VR-1516) and characterized by the Adenovirus Reference Material Working Group (ARMWG) to be 5.8 ⫻ 1011 particles/ml

(8) Codon-Optimization of Wild-Type Adeno-Associated Virus Capsid Sequences Enhances DNA Family Shuffling while Conserving FunctionalityMolecular Therapy - Methods & Clinical DevelopmentMarch 15, 2019Marti Cabanes-Creus, Samantha L. Ginn, Anais K. Amaya, Sophia H.Y. Liao, Adrian Westhaus, Claus V. Hallwirth, Patrick Wilmott, Jason Ward, Kimberley L. Dilworth, Giorgia Santilli, et al.fresh media added. To facilitate AAV library replication, we added WT human Adenovirus 5 (hAd5) (Cat #VR-1516; ATCC) at an MOI of 0.42 (based on 7-day median TC infective dose [TCID50]) to one of the plates. The

(9) Analysis of Replicative Intermediates of Adeno-associated Virus through Hirt Extraction and Southern BlottingBIO-PROTOCOLMay 5, 2017Martino Bardelli, Francisco Zarate-Perez, Leticia Agundez, Nelly Jolinon, R. Michael Linden, Carlos R. Escalante, Els Henckaerts23966-1) wt adenovirus serotype 5 (Graham and Prevec, 1991, available from ATCC, catalog number: VR-1516) Dulbecco modified Eagle’s medium (DMEM) (Thermo Fisher Scientific, catalog number: 31966021) Foetal

(10) Phosphatidylserine Exposure Controls Viral Innate Immune Responses by MicrogliaNeuronFebruary 8, 2017Tufail Y, Cook D, Fourgeaud L, Powers CJ, Merten K, Clark CL, Hoffman E, Ngo A, Sekiguchi KJ, O'Shea CC, et al.(PFU/ml):4.88×1010 Particle-to-PFU ratio: 24,This paper,N/A Adenovirus: Human adenovirus 5,ATCC,Cat#VR-1516 Plasmid: AdSyn Ad5 module pCOE1-038,"O’Shea and Powers, 2013",N/A Plasmid: AdSyn Ad5 module pCOASMM-025,"O’Shea

(11) A clinical protocol to inhibit the HGF/c-Met pathway for malignant mesothelioma with an intrapleural injection of adenoviruses expressing the NK4 geneSpringerPlusDecember 1, 2015Yuji Tada, Kenzo Hiroshima, Hideaki Shimada, Naoya Morishita, Toshiro Shirakawa, Kunio Matsumoto, Masato Shingyoji, Ikuo Sekine, Koichiro Tatsumi, Masatoshi Tagawareverse primer, 5′-CCGCACCTGGTTTTGCTT-3′, and 40 cycles at 62°C for annealing. Ad reference material (VR-1516, ATCC, Manassas, VA, USA) was used as a control.

(12) Nuclear actin and myosins in adenovirus infectionExperimental Cell ResearchNovember 1, 2015Beata Fuchsova, Leonid A. Serebryannyy, Primal de LanerolleSubconfluent cells were infected with wild-type human Adenovirus, Type 5 (Ad5 wildtype, ATCC, catalog No. VR-1516) at a multiplicity of infection of 120 infection units (IFU) per cell (1000 viral particles (VP)/cell).

(13) Recombinant adeno-vaccine expressing enterovirus 71-like particles against hand, foot, and mouth diseasePLoS neglected tropical diseasesApril 1, 2015Tsou YL, Lin YW, Shao HY, Yu SL, Wu SR, Lin HY, Liu CC, Huang C, Chong P, Chow YH.Vero cells based on the microcarrier cell culture bioprocess [29,30]. Human adenivirus 5 (Ad5; ATCC No. VR-1516) was purchased from ATCC and propagated in 293A cells. Virus stocks were stored at—80 °C. Virus stock

(14) Gene therapy for rhesus monkeys heterozygous for LDL receptor deficiency by balloon catheter hepatic delivery of helper-dependent adenoviral vectorGene therapyJanuary 1, 2015Oka K, Mullins CE, Kushwaha RS, Leen AM, Chan L.infectious titer of HDAd was defined by relative infectivity to an Adenovirus Type 5 Reference Material (VR-1516, ATCC) in competition to infect HEK293 cells45 except quantification of vectors by qPCR.46 Helper virus

(15) A Novel Selective LSD1/KDM1A Inhibitor Epigenetically Blocks Herpes Simplex Virus Lytic Replication and Reactivation from LatencymBioMarch 1, 2013Yu Liang, Debra Quenelle, Jodi L. Vogel, Cristina Mascaro, Alberto Ortega, Thomas M. Kristie10 postinfection at an LD50 (1.1 ⫻ 104 PFU/mouse). Mice (n ⫽ 5) were treated with OG-L002 at 20 mg/ 5 (VR-1516) were obtained from ATCC, rekg/day. spectively. Antibodies and primers. Antibodies and the sequences

(16) Rapid and Efficient Clearance of Blood-borne Virus by Liver Sinusoidal EndotheliumPLoS PathogensSeptember 29, 2011Latha P. Ganesan, Sudhasri Mohanty, Jonghan Kim, K. Reed Clark, John M. Robinson, Clark L. Anderson8.2×1011 IU/ml E2A qPCR method). Per the ATCC's Product Information Sheet for the Ad5 ARM (Cat. No. VR-1516), the IU titer on HEK 293 cells is 7.0×1010 Normalized Infectious Units (NIU)/mL, with 95% certainty

(17) Toll-Like Receptor 4-Mediated Activation of p38 Mitogen-Activated Protein Kinase Is a Determinant of Respiratory Virus Entry and TropismJournal of VirologyNovember 1, 2010David Marchant, Gurpreet K. Singhera, Soraya Utokaparch, Tillie L. Hackett, John H. Boyd, Zongshu Luo, Xiaoning Si, Delbert R. Dorscheid, Bruce M. McManus, Richard G. HegeleInstitutes of Health, Bethesda, MD) (45, 48). Human adenovirus (AdV) 5 was obtained from ATCC (catalog no. VR-1516). AdV-GFP (adenovirus 5-derived delta E1 and E3 vector with GFP expression driven from a cytomegalovirus

(18) Characterization of a Recombinant Adeno-Associated Virus Type 2 Reference Standard MaterialHuman Gene TherapyOctober 1, 2010Martin Lock et al.made on HeLaRC32 cells (CRL-2972; ATCC) (Chadeuf et al., 2000) and coinfected with adenovirus type 5 (VR-1516; ATCC). Fluorescence microscopy was used to count GFPexpressing cells at 72 hr postinfection. See the

(19) Wild-type adenoviruses from groups A–F evoke unique innate immune responses, of which HAd3 and SAd23 are partially complement dependentGene TherapyJune 1, 2008D M Appledorn et al.used in this study were purchased from the American Type Culture Collection (ATCC)—catalog numbers: VR-1516 (Ad5), VR-847 (HAd3), VR-592 (SAd23), VR-1109 (HAd31), VR-929 (HAd37), VR-930 (HAd41). Each virus was

(20) Quantification of High-Capacity Helper-Dependent Adenoviral Vector Genomes In Vitro and In Vivo, Using Quantitative TaqMan Real-Time Polymerase Chain ReactionHuman Gene TherapyMay 24, 2006M. Puntel et al.(ARM), that is, wild-type adenovirus serotype 5, was obtained from the American Type Culture Collection (VR-1516; ATCC, Manassas, VA). The biological titer that we obtained for the ARM was 4.92 × 1011 IU/ml (ATCC

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