Low Fluorescence Western Membrane (PVDF) from Abcam

Product Specs
ItemLow Fluorescence Western Membrane (PVDF)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab133411
Quantity10 1MEMB
ApplicationsWestern blot
TargetWestern blot membrane

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Beta-2 adrenergic receptor agonism alters astrocyte phagocytic activity and has potential applications to psychiatric diseaseDiscover Mental HealthNovember 30, 2023Ellen R. Bowen, Phillip DiGiacomo, Hannah P. Fraser, Kevin Guttenplan, Benjamin A. H. Smith, Marlene L. Heberling, Laura Vidano, Nigam Shah, Mehrdad Shamloo, Jennifer L. Wilson, et al.Waltham, MA, USA). The protein was transferred to a polyvinylidene difluoride membrane (Abcam, Cat: ab133411, Cambridge, UK) and incubated in Intercept (TBS) Blocking Buffer (Li-cor, Cat: 927-60,001, Lincoln,

(2) Hexavalent Chromium Inhibited Zebrafish Embryo Development by Altering Apoptosis- and Antioxidant-Related GenesCurr Issues Mol BiolAugust 18, 2023Khoa Dang Dang, Chi Nguyen Quynh Ho, Huy Duc Van, Son Thanh Dinh, Quynh Thi Truc Nguyen, Tram Thi Thuy Nguyen, Xuyen Thi Ngoc Kien, Tuyet Van Dao, Hung Viet Nong, Minh Thai Nguyen, et al.(ab119197, Abcam, Cambridge, MA, USA) for 2 h at 50 V. The protein was transferred to a PVDF membrane (ab133411, Abcam, Cambridge, MA, USA), and the membrane was blocked overnight at 4 °C with a blocking buffer (ab126587,

(3) The anti-inflammatory effects of photobiomodulation are mediated by cytokines: Evidence from a mouse model of inflammationFrontiers in NeuroscienceApril 6, 2023Shirin Shamloo, Erwin Defensor, Peter Ciari, Gaku Ogawa, Laura Vidano, Jennifer S. Lin, John A. Fortkort, Mehrdad Shamloo, Annelise E. BarronNW00107BOX Carlsbad, CA). The protein was transferred to a polyvinylidene difluoride membrane (Abcam, Cat: ab133411, Cambridge, United Kingdom) and incubated in Intercept® (TBS) Blocking Buffer (Li-cor, Cat: 927–60,001,

(4) Regulation of NOX/p38 MAPK/PPARα pathways and miR-155 expression by boswellic acids reduces hepatic injury in experimentally-induced alcoholic liver disease mouse model: novel mechanistic insightArchives of Pharmacal ResearchApril 1, 2023Rania M. Salama, Samah S. Abbas, Samar F. Darwish, Al Aliaa Sallam, Noura F. Elmongy, Sara A. El Wakeelgel was transferred onto a corresponding activated polyvinylidene difluoride (PVDF) membrane (Cat. # ab133411, Abcam, Cambridge, UK). Membranes’ blocking was then done by tris-buffered saline with Tween 20 (TBST)

(5) Olmesartan medoxomil self-microemulsifying drug delivery system reverses apoptosis and improves cell adhesion in trinitrobenzene sulfonic acid-induced colitis in ratsDrug DeliveryDecember 31, 2022Hussam Murad, Osama Ahmed, Thamer Alqurashi, Mostafa Hussienthe separated proteins were transferred onto the activated polyvinylidene difluoride membrane (Cat # ab133411, Abcam, Cambridge, UK). The whole membrane was blocked by 5% nonfat dry milk at room temperature for

(6) 6-Paradol Alleviates Testosterone-Induced Benign Prostatic Hyperplasia in Rats by Inhibiting AKT/mTOR AxisPlants (Basel)October 3, 2022Lenah S. Binmahfouz, Haifa Almukadi, Abdulmohsin J. Alamoudi, Ali M. El-Halawany, Hossam M. Abdallah, Mardi M. Algandaby, Gamal A. Mohamed, Sabrin R. M. Ibrahim, Faraj A. Alghamdi, Majed Al-Shaeri, et al.Tris-Glycine gel and transferred for 2 h using a semidry transfer cell (Bio-Rad) to a PVDF membrane (ab133411, ABCAM, Cambridge, UK). After separation, membranes were blocked with 5% non-fat dry milk in TBST (10

(7) Cordycepin Attenuates Testosterone-Induced Benign Prostatic Hyperplasia in Rats via Modulation of AMPK and AKT ActivationPharmaceuticsAugust 8, 2022Abdulmohsin J. Alamoudi, Sami A. Alessi, Waleed Y. Rizg, Abdulmajeed M. Jali, Awaji Y. Safhi, Fahad Y. Sabei, Sameer Alshehri, Khaled M. Hosny, Ashraf B. Abdel-Naimthe separated proteins were transferred onto an activated polyvinylidene difluoride membrane (Cat # ab133411, Abcam, Cambridge, UK). The membranes were blocked by 5% non-fat milk in TBST for 1 h at room temperature

(8) The immune checkpoint ICOSLG is a relapse-predicting biomarker and therapeutic target in infant t(4;11) acute lymphoblastic leukemiaiScienceJuly 15, 2022Marius KÃ¼lp, Anna Lena Siemund, Patrizia Larghero, Alissa Dietz, Julia Alten, Gunnar Cario, Cornelia Eckert, AurÃ©lie Caye-Eude, HÃ©lÃ¨ne CavÃ©, Michela Bardini, et al.Standard Broad Range (P7719S, NEB). Proteins were blotted onto Low Fluorescence Western Memranes PVDF (ab133411, Abcam) in Towbin buffer (25 mM Tris, 192 mM Glycin, 15% methanol, 0.01% SDS, pH = 8.3) at 110 V for

(9) Knockdown of LncRNA NEAT1 inhibits myofibroblast activity in oral submucous fibrosis through miR-760/TPM1 axisJournal of Dental SciencesApril 1, 2022Wei Li, Bin Chengseparated by 10% SDS-PAGE gels and transferred to polyvinylidene difluoride (PVDF) membranes (Abcam, #ab133411). Then, PVDF membranes were conducted in 5% Bovine Serum Albumin (BSA, Sangon Biotech, Shanghai, China,

(10) Ketamine inhibits TNF-α-induced cecal damage by enhancing RIP1 ubiquitination to attenuate lethal SIRSCell death discoveryFebruary 19, 2022Deng B, Yang D, Wu H, Wang L, Wu R, Zhu H, Huang A, Song J, Cai T, Liu S, et al.marker (26616; Thermo Fisher Scientific, Inc.), proteins were transferred onto PVDF membranes (cat. no., ab133411; Abcam). 5% skim milk was adopted to block the membranes for 40 min to decrease nonspecific binding,

(11) Differential ER stress as a driver of cell fate following ricin toxin exposureFASEB bioAdvancesJanuary 1, 2022Peterson-Reynolds C, Mantis NJ.manufacturer's protocol. For western blotting, proteins were transferred to PVDF membranes (Abcam Cat# ab133411) in a high field wet tank transfer. The membranes were retrieved, rinsed briefly in water, and fixed

(12) Simulated Microgravity Induces the Proliferative Inhibition and Morphological Changes in Porcine Granulosa CellsCurrent Issues in Molecular BiologyDecember 10, 2021Truong Xuan Dai et al.Cambridge, MA, USA) for 2 h at 50 V. The protein was transferred to a methanol-treated PVDF membrane (ab133411, Abcam, Cambridge, MA, USA). The membrane was treated with blocking buffer (ab126587, Abcam, Cambridge,

(13) Altered H3 histone acetylation impairs high-fidelity DNA repair to promote cerebellar degeneration in spinocerebellar ataxia type 7Cell reportsNovember 30, 2021Switonski PM, Delaney JR, Bartelt LC, Niu C, Ramos-Zapatero M, Spann NJ, Alaghatta A, Chen T, Griffin EN, Bapat J, et al.a 4%–12% SDS-PAGE gel (Invitrogen, cat# NW04125BOX) and transferred onto PVDF membrane (Abcam, cat# ab133411-101MEMB). The membrane was blocked in 5% dry milk (Genesee, cat#20-241). Antibodies used were as follows:

(14) Mokko Lactone Attenuates Doxorubicin-Induced Hepatotoxicity in Rats: Emphasis on Sirt-1/FOXO1/NF-κB AxisNutrientsNovember 19, 2021Alaa Sirwi, Rasheed A. Shaik, Abdulmohsin J. Alamoudi, Basma G. Eid, Ahmed K. Kammoun, Sabrin R. M. Ibrahim, Gamal A. Mohamed, Hossam M. Abdallah, Ashraf B. Abdel-Naimlysates (50 µg) were separated by a 10% Tris-Glycine gel and then placed on a PVDF membrane (Cat. No.: ab133411, Abcam, Cambridge, UK). using a semidry transfer cell (Bio-Rad) for 2 h. After blocking with 5% nonfat

(15) Age-related neuroinflammation and pathology in the locus coeruleus and hippocampus: beta-adrenergic antagonists exacerbate impairment of learning and memory in aged miceNeurobiology of agingOctober 1, 2021Evans AK, Park HH, Saw NL, Singhal K, Ogawa G, Leib RD, Shamloo M.NW00107BOX Carlsbad, CA). The protein was transferred to a polyvinylidene difluoride membrane (Abcam, Cat: ab133411, Cambridge, UK) and incubated in Intercept® (TBS) Blocking Buffer (Li-cor, Cat: 927–60001, Lincoln,

(16) Simulated Microgravity Inhibits the Proliferation of Chang Liver Cells by Attenuation of the Major Cell Cycle Regulators and Cytoskeletal ProteinsInternational Journal of Molecular SciencesApril 27, 2021Chi Nguyen Quynh Ho, Minh Thi Tran, Chung Chinh Doan, Son Nghia Hoang, Diem Hong Tran, Long Thanh LeV. The protein was transferred to Int. J. Mol. Sci. 2021, 22, 4550 11 of 14 a PVDF membrane (ab133411, Abcam, Cambridge, MA, USA), and the membrane was blocked overnight at 4 ◦ C with blocking buffer (ab126587,

(17) Neuroprotective effect of ketamine against TNF‐α‐induced necroptosis in hippocampal neuronsJournal of Cellular and Molecular MedicineApril 1, 2021Lu Wang, Bin Deng, Panpan Yan, Huanghui Wu, Chunhui Li, Hongrui Zhu, Jiwei Du, Lichao Houthe marker (26616; Thermo Fisher Scientific, Inc) and then transferred onto PVDF membranes (cat. no., ab133411; Abcam). The membranes were blocked using 5% skim milk for 40 minutes to reduce non‐specific binding

(18) Beta-adrenergic receptor antagonism is proinflammatory and exacerbates neuroinflammation in a mouse model of Alzheimer's DiseaseNeurobiology of diseaseDecember 1, 2020Evans AK, Ardestani PM, Yi B, Park HH, Lam RK, Shamloo M.NW00107BOX Carlsbad, CA). The protein was transferred to a polyvinylidene difluoride membrane (Abcam, Cat: ab133411, Cambridge, UK) and incubated in Intercept® (TBS) Blocking Buffer (Li-cor, Cat: 927–60001, Lincoln,

(19) Simulated Microgravity Reduces Proliferation and Reorganizes the Cytoskeleton of Human Umbilical Cord Mesenchymal Stem CellsPhysiological ResearchOctober 31, 2020H N Quynh Chi, H Nghia Son, D Chinh Chung, L D HUAN, T Hong Diem, L T LONGOptiblot SDS Run Buffer (ab119197, Abcam) for 2 h at 50 V. Protein was transferred to a PVDF membrane (ab133411; Abcam), which was blocked overnight at 4 °C with a blocking buffer (ab126587; Abcam). The membrane

(20) Naringin Protects Against Interleukin 1β (IL-1β)-Induced Human Nucleus Pulposus Cells Degeneration via Downregulation Nuclear Factor kappa B (NF-κB) Pathway and p53 ExpressionMedical Science MonitorDecember 25, 2019Gang Gao, Feng Chang, Ting Zhang, Xinhu Huang, Chen Yu, Zhaolin Hu, Mingming Ji, Yufen Duan(CC BY-NC-ND 4.0) 4–20% (ab119209, Abcam, UK) and transferred onto polyvinylidene difluoride (PVDF, ab133411, Abcam, UK) membranes, which were blocked by 5% non-fat milk at room temperature for 15 minutes and

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Low Fluorescence Western Membrane (PVDF) from Abcam

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Low Fluorescence Western Membrane (PVDF) from Abcam

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