Description
The SuperScript Double-Stranded cDNA Synthesis Kit contains all of the reagents (except an oligo (dT)-containing primer) necessary to make high-quality, double-stranded cDNA from total RNA or poly A+-selected RNA (mRNA). The advanced capabilities of SuperScript II Reverse Transcriptase, with its reduced RNase H activity, maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis. Features of the SuperScript Double-Stranded cDNA Synthesis Kit include: Reliability—each buffer, reagent, and enzyme in the kit is of the same high quality you expect from us Performance—SuperScript II is an enzyme you can trust for high-yield, high-quality, general-purpose, first-strand synthesis. Simple, reliable, and trouble-free The SuperScript Double-Stranded cDNA Synthesis Kit offers a simple, reliable, and trouble-free method by which RNA templates can be converted to cDNA for use in cloning and library construction experiments. The reactions described in the SuperScript Double-Stranded cDNA Synthesis Kit protocol are designed to convert 25-50 g total RNA, or 0.2-5 g mRNA, into first- and second-strand cDNA (supply your own primer). Enjoy the benefits of a tested protocol and reagents of consistent quality. To get the best possible cDNA product using this kit, we recommend the use of a high-quality RNA substrate prepared using TRIzol RNA Isolation Reagents