Fig 1: Rapamycin-induced dimerization to drive nuclear export.(A) Schematic of LR recombination reaction used to create pEpic CMV:OGT1-mCherry-FRB-HA. (B) Schematic of rapamycin-induced dimerization with FKBP-NES to drive nuclear export. (C) Time-lapse imaging (S1 Movie) of mCherry in HEK293T cells transfected with pEpic CMV:OGT1-mCherry-FRB-HA and FKBP-NES. Rapamycin was added at time 0. White arrows mark cell nuclei.
Supplier Page from DNASU for OGT (Homo sapiens) in pDONR221 (Gateway donor/master vector)