Fig 1: A and B: MDA-MB-231 cells treated with 10 μM JNK inhibitor SP 600125 (SP), 20 μM MEK inhibitor PD 98059 (PD) or 5 μM p38 inhibitor SB 202190 (SB) for 24 h showed significantly decreased mRNA levels of MMP-1 and MMP-3 relative to the control cells (CON). C: MDA-MB-231 cells with increased LPP1 expression showed decreased cFOS and cJUN in cytoplasm (C) and nuclei (N) compared with control cells (CON). The level of FRA1 was increased in cytoplasm but not affected in nuclei by LPP1. D: Protein levels of cFOS, cJUN, or FRA1 in MDA-MB-231 cells overexpressing these proteins (AP-1) and in control cells (CON). E and F: Transient expressions of cFOS, cJUN or FRA1 significantly increased mRNA levels of MMP-1 and MMP-3 in MDA-MB-231 cells. Results are means and SEM from three independent experiments. Results were analyzed by ANOVA. *P < 0.05, ** P < 0.01, *** P<0.001.
Fig 2: A - C: EGF (100 ng/ml) induced mRNA expression of cFOS (FOS), cJUN (JUN) and FRA1 (FOSL1) in MDA-MB-231 cells with or without increased expression of LPP1. D: EGF (100 ng/ml) induced expression of cFOS, cJUN and FRA1 in nuclei (N) and cytoplasm (C) from MDA-MB-231 cells with or without increased expression of LPP1. E - J: Quantification for EGF-induced cFOS (E and H), cJUN (F and I) and FRA1 (G and J) from panel D. K and L: EGF-induced increases in concentrations of MMP-1 and MMP-3 in the conditioned media of MDA-MB-231 cells were suppressed by LPP1. Results are means and SEM from three independent experiments. Results were analyzed by ANOVA. *P < 0.05 relative to control, ** P < 0.01, NS: not significant.
Fig 3: A and B: Human breast tumors (ER+: n=12, HER2+: n=11, ER/PR+: n=12, ER/HER2+: n=12, ER/HER2/PR+: n=12, triple negative: n=11) had lower mRNA levels for LPP1 and LPP3 relative to normal breast tissue obtained from breast reduction surgery (n=12). Low LPP1 (C) but not low LPP3 (D) was associated with decreased disease-free survival rate. E and F: Breast tumors (n=12) had higher MMP-1 and MMP-3 than normal breast tissue (n=12) G - L: Breast tumors (n=6) express higher levels of cFOS, cJUN, FRA1, cycline D1, and cyclin D3 than normal breast tissue (n=6). Results are means and SEM and analyzed by Student's t-test or ANOVA. The disease-free survival rate was analyzed with Gehan-Breslow-Wilcoxon test. *P < 0.05, ** P < 0.01, *** P < 0.001.
Supplier Page from DNASU for FOSL1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)