Bsu36I from New England Biolabs

Product Specs
ItemBsu36I
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0524L
Quantity5000 units
Concentration10 u/µL
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

CC/TNAGG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Solid-to-liquid phase transition in the dissolution of cytosolic misfolded-protein aggregatesiScienceDecember 15, 2023Alexis Tomaszewski, Rebecca Wang, Eduardo Sandoval, Jin Zhu, Jian Liu, Rong Liworkstation (Eppendorf) and 150 μL of refreshed logarithmic phase cultures plus 0.2 μg of Bsu36I (NEB, Cat #: R0524S) linearized FlucSM-GFP-URA3 DNA were added to the Frozen-EZ Yeast solution mix. The transformation mixtures

(2) Binding of the nuclear ribonucleoprotein family member FUS to RNA prevents R-loop RNA:DNA hybrid structuresThe Journal of biological chemistryOctober 1, 2023Thompson VF, Wieland DR, Mendoza-Leon V, Janis HI, Lay MA, Harrell LM, Schwartz JC.and the gene inserted for RNA transcription was Flag-FUS (13). Bsu36I restriction enzyme (50 U, NEB, R0524S) was used to linearize plasmid (10 μg) by incubating at 37 °C for a minimum of 2 h in 1× CutSmart buffer

(3) Screening of Membrane Protein Production by Comparison of Transient Expression in Insect and Mammalian CellsBiomoleculesMay 11, 2023Jagan Mohan Kaipa, Ganna Krasnoselska, Raymond J. Owens, Joop van den Heuveltransfer plasmid (500/750 ng) was added to Bsu36I (New England Biolabs, Frankfurt-am-Main, Germany, #R0524S), linearized empty DsRed bacmid (500 ng) (the bacmid was a kind gift from Prof. Ian Jones) diluted in

(4) Bacterial genome engineering using CRISPR RNA-guided transposasesbioRxivMarch 25, 2023Diego R. Gelsinger, Phuc Leo H. Vo, Sanne E. Klompe, Carlotta Ronda, Harris Wang, Samuel H. SternbergR3140S/L/T/M)XhoI restriction enzyme (NEB cat. no. R0146S/M)Bsu36I restriction enzyme (NEB cat. no. R0524S/L)SalI restriction enzyme (NEB cat. no. R0138S/T/L/M)CutSmart or rCutSmart buffer, 5X (included with

(5) Thermostable negative-marker foot-and-mouth disease virus serotype O induces protective immunity in guinea pigsApplied Microbiology and BiotechnologyFebruary 1, 2023Huildore Bommanna Ranjitha, Valiya Valappil Dhanesh, Madhusudan Hosamani, B. P. Sreenivasa, Uzma Jabeen, Jitendra Kumar Biswal, P. Saravanan, Aniket Sanyal, Veerakyathappa Bhanuprakash, Suresh H. Basagoudanavarwas digested with Nhe I (New England Biolabs, USA, #R0131S) and Bsu36 I (New England Biolabs, USA, #R0524S) and used to replace the corresponding fragment in pT7-OR2/75. The new recombinant plasmid was designated

(6) Human ALS/FTD brain organoid slice cultures display distinct early astrocyte and targetable neuronal pathologyNature neuroscienceNovember 1, 2021Szebényi K, Wenger LMD, Sun Y, Dunn AWE, Limegrover CA, Gibbons GM, Conci E, Paulsen O, Mierau SB, Balmus G, et al.Blood and Tissue kit (69506). DNA (5 µg) was restriction digested with Bsu36I (New England Biolabs, R0524S) and ran on 0.8% agarose gels accompanied by molecular weight markers II (Roche, 11218590910) and III

(7) ADAR2 mislocalization and widespread RNA editing aberrations in C9orf72-mediated ALS/FTDActa neuropathologicaJuly 1, 2019Moore S, Alsop E, Lorenzini I, Starr A, Rabichow BE, Mendez E, Levy JL, Burciu C, Reiman R, Chew J, et al.NEBuilder HiFi DNA Assembly Cloning Kit (NEB-E2621S), with ADAR2-pEZ-Lv111 destination vector linearized with XbaI and Bsu36I (NEB-R0524S) and gel purified. Fragments were amplified via PCR (Thermo Scientific-F549S) using ADAR2-pEZ-Lv111

(8) Precision Tagging: A Novel Seamless Protein Tagging by Combinational Use of Type II and Type IIS Restriction EndonucleasesBIO-PROTOCOLFebruary 5, 2018Zhen Xu, Yan-Ning Rui, John P. Hagan, Dong H. KimR3136S)SalI-HF (New England Biolabs, catalog number: R3138S)Bsu36I (New England Biolabs, catalog number: R0524S) Type IIS restriction endonucleases such as: BsaI-HF (New England Biolabs, catalog number: R3535S)BbsI-HF

(9) Gene therapy for human ovarian cancer cells using efficient expression of Fas gene combined with γδT cellsMolecular medicine reportsOctober 1, 2017Lin J, Zeng D, He H, Tan G, Lan Y, Jiang F, Sheng S.enzyme digestion using Nhe I (catalog no. R0131S; New England BioLabs, Inc.) and Bsu36I (catalog no. R0524S; New England BioLabs, Inc.). For enzyme digestion, PCR products were digested with XbaI and Kpn I (10

(10) Cell line with endogenous EGFRvIII expression is a suitable model for research and drug development purposesOncotargetMay 31, 2016Stec WJ, Rosiak K, Siejka P, Peciak J, Popeda M, Banaszczyk M, Pawlowska R, Treda C, Hulas-Bigoszewska K, Piaskowski S, et al.Cat. no. OHS5922) previously digested with Bsu36I/SnaBI enzymes (both New England Biolabs, Cat. no. R0524 and R0130, respectively). Electrophoretic analysis and DNA sequencing were performed to verify the resulting

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Bsu36I

Bsu36I from New England Biolabs

Sequence

New England Biolabs

Citations (10)

Reviews

Bsu36I from New England Biolabs

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