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BstBI
BstBI
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BstBI
BstBI from New England Biolabs
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Citations:
(15)
Supplier Page
Supplier Page from
New England Biolabs for
BstBI
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
BstBI
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0519S
Concentration
20 u/µL
Quantity
2500 units
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
TT/CGAA
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (15)
(1)
Molecular basis for proofreading by the unique exonuclease domain of Family-D DNA polymerases
Nat Commun
December 14, 2023
Leonardo Betancurt-Anzola, Markel Martínez-Carranza, Marc Delarue, Kelly M. Zatopek, Andrew F. Gardner, Ludovic Sauguet
the reaction was quenched with equal volumes of 50 mM EDTA. To the remaining 30 µL, 4 units of
BstBI
(
R0519
New England Biolabs
Inc., Ipswich MA) was added, and further incubated at 65 °C for 15 min. 10 µL of
(2)
Paired yeast one-hybrid assays to detect DNA-binding cooperativity and antagonism across transcription factors
Nature Communications
October 18, 2023
Anna Berenson, Ryan Lane, Luis F. Soto-Ugaldi, Mahir Patel, Cosmin Ciausu, Zhaorong Li, Yilin Chen, Sakshi Shah, Clarissa Santoso, Xing Liu,
et al.
single-cutter restriction enzymes (
New England Biolabs
R0520L
,
R0146L
,
R3127S
,
R0581S
,
R0193L
,
R0114S
,
R0187S
,
R0519L
). The pWM#2 and pWM#3 plasmids for each promoter were integrated simultaneously into the Y1Has2
yeast
(3)
Transcription activator-like effector protects bacterial endosymbionts from entrapment within fungal hyphae
Current Biology
July 10, 2023
Ingrid Richter, Philipp Wein, Zerrin Uzum, Claire E. Stanley, Jana Krabbe, Evelyn M. Molloy, Nadine Moebius, Iuliia Ferling, Falk Hillmann, Christian Hertweck
Biolabs,CAT#E1510
NdeI
,
New England Biolabs
,CAT#
R0111L
AflII
,
New England Biolabs
,CAT#
R0520L
BstBI
,
New England Biolabs
,CAT#
R0519L
Chloramphenicol,Roth,CAT#3886.1 Potato Dextrose Broth,"Becton, Dickinson & Co.",CAT#254920 NaCl,Roth,CAT#3957.1 SYTO™
(4)
Use of a Bacterial Artificial Chromosome to Generate Recombinant SARS-CoV-2 Expressing Robust Levels of Reporter Genes
Microbiology Spectrum
December 21, 2022
Chengjin Ye, Luis Martinez-Sobrido
no.
R3733L
),
BsmBI
(
New England Biolabs
, catalog no.
R0739L
),
BstBI
(
New England Biolabs
, catalog no.
R0519L
),
Cell culture
water (
Thermo Fisher Scientific
, catalog no.
A1287301
), chloramphenicol (
Corning
, catalog
(5)
Multifaceted role of RNA editing in promoting loss-of-function of PODXL in cancer
iScience
August 19, 2022
Ting Fu, Tracey W. Chan, Jae Hoon Bahn, Tae-Hyung Kim, Amy C. Rowat, Xinshu Xiao
Green",
Sartorius
,Cat#
4440
AgeI-HF
,
NEB
,Cat#
R3552S
EcoRI-HF
,
NEB
,Cat#
R3101S
NotI-HF
,
NEB
,Cat#
R3189S
BstBI
,
NEB
,Cat#
R0519S
XbaI
,
NEB
,Cat#
R0145S
,, Critical commercial
assays
,Critical commercial
assays
,Critical commercial
(6)
Mammalian Ddi2 is a shuttling factor containing a retroviral protease domain that influences binding of ubiquitylated proteins and proteasomal degradation
The Journal of biological chemistry
May 1, 2022
Collins GA, Sha Z, Kuo CL, Erbil B, Goldberg AL.
Ddi2
from pReceiver-MO2-
Ddi2
was excised by digestion with
BstBI
(
New England Biolabs
; catalog no.:
R0519S
) and
NotI
(
NEB
; catalog no.:
R0189S
) and cloned into the
BstBI
/
NotI
sites of the
pReceiver-M06
plasmid
(7)
Cloning of DNA oligo pools for in vitro expression
STAR Protocols
April 25, 2022
Anna Uzonyi, Ronit Nir, Schraga Schwartz
Herculase II Fusion DNA polymerase
,
Agilent
,#
600679
FD buffer,
Thermo Fisher Scientific
,#
B64
XbaI,Fermentas,#ER0681
BstBI
,
NEB
,#
R0519S
BcuI (SpeI),
Thermo Fisher Scientific
,#
FD1254
FastAP,
Thermo Fisher Scientific
,#
EF0651
GlycoBlue,
Thermo Fisher Scientific
(8)
AdenoBuilder: A platform for the modular assembly of recombinant adenoviruses
STAR Protocols
March 18, 2022
Yumi Jang, Fred Bunz
Regular",
Corning
,
35-010-CV
Penicillin Streptomycin,
Gibco
,
15140122
BstBI
enzyme,
New England Biolabs
,
R0519S
NEBuilder HiFi DNA Assembly Master Mix
,
New England Biolabs
,
E2621S
PBS pH7.4 (1×),
Gibco
,
10010023
"
Trypsin-EDTA (0.05%), phenol red
(9)
Epigenetic Mechanisms Contribute to Evolutionary Adaptation of Gene Network Activity under Environmental Selection
Cell Reports
October 27, 2020
Xinyue Luo, Ruijie Song, David F. Moreno, Hong-Yeoul Ryu, Mark Hochstrasser, Murat Acar
Proteins",,
Gibson Assembly® Master Mix
,
New England BioLabs
,
E2611S
Restriction Enzyme:
BstBI
,
New England BioLabs
,
R0519S
iTaqTM Universal SYBR® Green Supermix,
Bio-Rad
,
1725120
Protein G-Sepharose,
GE Healthcare
,
17-0618-01
Pronase
,
Roche
,11
(10)
Guide RNAs with embedded barcodes boost CRISPR-pooled screens
Genome Biology
December 1, 2019
Shiyou Zhu, Zhongzheng Cao, Zhiheng Liu, Yuan He, Yinan Wang, Pengfei Yuan, Wei Li, Feng Tian, Ying Bao, Wensheng Wei
constructed by changing the position of the BsmBI (
Thermo Scientific
™,
ER0451
) site using
BstBI
(
NEB
,
R0519
) and
XhoI
(
NEB
, R0146) from Plenti-sgRNA-Lib (Addgene, #53121). sgRNA- and sgRNAiBAR-expressing sequences
(11)
A Novel Animal Model of Emphysema Induced by Anti-Elastin Autoimmunity
Journal of immunology (Baltimore, Md. : 1950)
July 15, 2019
Gu BH, Sprouse ML, Madison MC, Hong MJ, Yuan X, Tung HY, Landers CT, Song LZ, Corry DB, Bettini M,
et al
.
insertion of each TCRα and TCRβ chain was confirmed with specific restriction sites digestion:
BstBI
(
NEB
#
R0519
) and
MfeI
(
NEB
#R3589) for TCRα, and
SnaBI
(
NEB
#
R0130
) and
SacII
(
NEB
#R0157) for TCRβ. Following confirmation
(12)
Naturally Occurring Off-Switches for CRISPR-Cas9
Cell
December 15, 2016
April Pawluk et al.
,
New England Biolabs
,#
R0138S
XhoI
,
New England Biolabs
,#
R0146S
BsmBI
,
New England Biolabs
,#
R0580S
BstBI
,
New England Biolabs
,#
R0519S
ScaI-HF
,
New England Biolabs
,#
R3122S
Tobacco
Etch
Virus
(
TEV) protease
,A. Davidson Lab, Ni-NTA agarose
(13)
Rapid identification and expression of human TCRs in retrogenic mice☆
Journal of Immunological Methods
December 1, 2016
Maran L. Sprouse, Gabriele Blahnik, Thomas Lee, Natalie Tully, Pinaki Banerjee, Eddie A. James, Maria J. Redondo, Matthew L. Bettini, Maria Bettini
TCR. Purified
PCR
products from the beta nested reaction and the TCR-pMIA vector were digested with
BstbI
(
NEB
#
R0519S
) and
MfeI
(
NEB
#
R3589L
). The digested
PCR
products were purified using a DNA purification kit (Zymo
(14)
Cell line with endogenous EGFRvIII expression is a suitable model for research and drug development purposes
Oncotarget
May 31, 2016
Stec WJ, Rosiak K, Siejka P, Peciak J, Popeda M, Banaszczyk M, Pawlowska R, Treda C, Hulas-Bigoszewska K, Piaskowski S,
et al
.
cloning
with the use of
NheI
/
BstBI
restriction enzymes (both
New England Biolabs
, Cat. no. R0131 and
R0519
, respectively), Shrimp Alkaline Phosphatase (
New England Biolabs
, Cat. no.
M0371
) and
T4 DNA Ligase
(15)
A versatile element for gene addition in bacterial chromosomes
Nucleic Acids Research
February 1, 2012
Marion H. Sibley, Elisabeth A. Raleigh
but can, of course, be scaled up. Protocol 1.Digest plasmid with
BstBI
restriction endonuclease (
NEB
#
R0519
):2.Nick the
BstBI
-linearized vector with
Nt.BbvCI
(
NEB
#
R0632
) by adding 2 µl (20 U) of
Nt.BbvCI
(10
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BstBI from New England Biolabs
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