Welcome Guest
Sign In
Register
Products
Popular Categories
Antibody Search
ELISA Kit Search
Biomolecules
Flow Cytometers
Thermal Cyclers
Microscopes
Transfection Reagents
Featured Products
New Products
Articles
Resources
eBooks
Bench Tips
Tech Insights
Editorial Articles
News
Product Reviews
Future Lab
Multimedia
Biocompare Webinars
Immunotherapy
Reproducibility
Transformative Technologies
Podcasts
Tech Talks
Bench Tip Videos
All Videos and Webinars
Life Science Webinars
Write a Review
Antibodies
Kits/Reagents
Equipment
Services
Resources
Promotions
Events
AACR SPOTLIGHT
Register
Sign In
Biocompare
Products
Cell Biology
Biomolecules
Biomolecules
Trypsin/Lys-C Mix, Mass Spec Grade
Supplier Images
Trypsin/Lys-C Mix, Mass Spec Grade
Trypsin/Lys-C Mix, Mass Spec Grade
Trypsin/Lys-C Mix, Mass Spec Grade from Promega
Be the first to write a review!
Citations:
(233)
Supplier Page
Supplier Page from
Promega for
Trypsin/Lys-C Mix, Mass Spec Grade
Get Pricing
Ask a Question
Description
Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Trypsin/Lys-C Mix, Mass Spec Grade
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
V5071
Quantity
20microg
Add to Compare List
Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (233)
(1)
Protocol for establishing a protein interactome based on close physical proximity to a target protein within live budding yeast
STAR Protocols
December 1, 2023
Janhavi A. Kolhe, Neethu L. Babu, Brian C. Freeman
XL-1500 with 365 nm bulbs,Spectro-UV,Cat#1195T76 "
Trypsin/Lys-C Mix, Mass Spec Grade
",
Promega
,Cat#
V5071
"
AssayMAP C18 cartridges, 96/pk
",
Agilent
,Cat#
5190-6532
AssayMAP Bravo Protein and Peptide Sample Prep
(2)
Unravelling the Proteomic Profiles of Bovine Colostrum and Mature Milk Derived from the First and Second Lactations
Foods
November 7, 2023
Zhen Feng, Yan Shen, Gongjian Fan, Tingting Li, Caie Wu, Yuhui Ye
of samples were detected based on the Bradford method [16] and then normalized to 1 mg/mL. Trypsin (
V5071
,
Promega
, Madison, WI, USA) was added at a ratio of 50:1 (w/w) and the solution was incubated at 37
(3)
Identification of a Novel Acid Sphingomyelinase Activity Associated with Recombinant Human Acid Ceramidase
Biomolecules
November 6, 2023
Xingxuan He, Edward H. Schuchman
final concentration of 1 M. Half of the sample was digested overnight with trypsin/LysC (
Promega
, #
V5072
) at a ratio of
1:10
(protein/enzyme; w:w) and the other half was digested overnight with
Asp
-N (
Promega
,
(4)
Functional diversification despite structural congruence in the HipBST toxin-antitoxin system of Legionella pneumophila
mBio
October 31, 2023
Jordan D. Lin, Peter J. Stogios, Kento T. Abe, Avril Wang, John MacPherson, Tatiana Skarina, Anne-Claude Gingras, Alexei Savchenko, Alexander W. Ensminger
(25°C, 45 min). Samples were digested for 2 h at 25°C with constant agitation with a Trypsin/LysC mix (
V5071
,
Promega
) and overnight at 25°C with proteomics-grade porcine trypsin (
T6567
,
Sigma-Aldrich
). For both
(5)
The protein phosphatase PPKL is a key regulator of daughter parasite development in Toxoplasma gondii
mBio
October 25, 2023
Chunlin Yang, Emma H. Doud, Emily Sampson, Gustavo Arrizabalaga
phosphoproteomics,
1:70
protease: substrate ratio,
mass spectrometry
grade,
Promega Corporation
, Cat No:
V5072
). Digestions were acidified with trifluoroacetic acid (TFA, 0.5% vol/vol) and desalted on Sep-Pak Vac
(6)
Native lamin A/C proteomes and novel partners from heart and skeletal muscle in a mouse chronic inflammation model of human frailty
Frontiers in Cell and Developmental Biology
October 23, 2023
Fatima D. Elzamzami, Arushi Samal, Adith S. Arun, Tejas Dharmaraj, Neeti R. Prasad, Alex Rendon-Jonguitud, Lauren DeVine, Jeremy D. Walston, Robert N. Cole, Katherine L. Wilson
Trypsin/LysC mixture [40 ug proteases in 1.2 mL of 100 mM triethylammonium bicarbonate (TEAB;
Promega
#
V5071
) or approximately 3.33 ug protease per sample]. Individual samples (40 ug) were labeled with a unique
(7)
Understanding COVID-19 progression with longitudinal peripheral blood mononuclear cell proteomics: Changes in the cellular proteome over time
iScience
October 20, 2023
Giuseppe Gianini Figueirêdo Leite, Milena Karina Colo Brunialti, Paula M. Peçanha-Pietrobom, Paulo R. Abrão Ferreira, Jaquelina Sonoe Ota-Arakaki, Edecio Cunha-Neto, Bianca Lima Ferreira, Graziella E. Ronsein, Alexandre Keiji Tashima, Reinaldo Salomão
34851 DL-Dithiothreitol,Sigma-Aldrich,Cat# D0632
Iodoacetamide
,
Sigma-Aldrich
,Cat#
I1149
Trypsin/Lys-C Mix,
Promega
,Cat#
V5071
50% Hydroxylamine for TMT experiments
,
Thermo Scientific
,Cat#
90115
Urea
,
Sigma-Aldrich
,Cat# 51456 Thiourea,Sigma-Aldrich,Cat#
(8)
Loss of succinyl-CoA synthetase in mouse forebrain results in hypersuccinylation with perturbed neuronal transcription and metabolism
Cell Reports
October 17, 2023
Makayla S. Lancaster, Byungwook Kim, Emma H. Doud, Mason D. Tate, Ahmad D. Sharify, Hongyu Gao, Duojiao Chen, Ed Simpson, Patrick Gillespie, Xiaona Chu,
et al.
Aldrich,Cat.No.: C4706 Chloroacetamide,Sigma Aldrich,Cat.No.: C0267 Trypsin/Lys-C,
Promega
,Cat.No.:
V5072
NP-40,IBI Scientific,Cat.No.: 9016–45-9 Trifluoroacetic acid,Alfa Aesar,Cat.No.: LO6374 "Tandem Mass
(9)
Taste papilla cell differentiation requires the regulation of secretory protein production by ALK3-BMP signaling in the tongue mesenchyme
Development
September 15, 2023
Mohamed Ishan, Zhonghou Wang, Peng Zhao, Yao Yao, Steven L. Stice, Lance Wells, Yuji Mishina, Hong-Xiang Liu
phosphine hydrochloride, alkylated with 13.75 mM of iodoacetamide and digested with trypsin/Lys-C mix (
V5071
,
Promega
). The resulting peptides were cleaned up with Acclaim PepMap 100 C18 spin columns (
SEM
SS18V,
(10)
NLRP14 Safeguards Calcium Homeostasis via Regulating the K27 Ubiquitination of Nclx in Oocyte‐to‐Embryo Transition
Advanced Science
September 1, 2023
Tie‐Gang Meng, Jia‐Ni Guo, Liu Zhu, Yike Yin, Feng Wang, Zhi‐Ming Han, Lei Lei, Xue‐Shan Ma, Yue Xue, Wei Yue,
et al.
and alkylated with 10 mM iodoacetamide (
Sigma
,
I1149
). After digestion by Trypsin/Lys‐c Mix (
Promega
,
V5071
) overnight, the peptide solution was desalted using SOLAµ HRP SPE spin plates (Thermo Scientific, 60209‐001).
(11)
Protocol for deep proteomic profiling of formalin-fixed paraffin-embedded specimens using a spectral library-free approach
STAR Protocols
September 1, 2023
Rajesh Kumar Soni
(NaOH),Sigma-Aldrich,221465-500G Sera-Mag SpeedBeads,
Thermo Scientific
,4515-2105-050250
Trypsin
/
Lys-C Mass spec grade
,
Promega
,
V5071
"Trifluoroacetic acid,
sequencing
grade (TFA)",
Thermo Fisher Scientific
,
28904
Ethylacetate,Millipore
(12)
Characterization of endoplasmic reticulum-associated degradation in the human fungal pathogen Candida albicans
PeerJ
August 25, 2023
Ellen M. Doss, Joshua M. Moore, Bryce H. Harman, Emma H. Doud, Eric M. Rubenstein, Douglas A. Bernstein
protein digestion
at 37 °C (
1:70
protease:substrate ratio,
Mass Spectrometry
grade,
Promega Corporation
, Cat No:
V5072
.). Peptide
purification
and
labeling
: Digestions were acidified with trifluoroacetic acid (TFA, 0.5%
(13)
A micro-flow, high-pH, reversed-phase peptide fractionation and collection system for targeted and in-depth proteomics of low-abundance proteins in limiting samples
MethodsX
July 31, 2023
Marta Zurawska, Mark Basik, Adriana Aguilar-Mahecha, Michal Dadlez, Dominik Domanski
purchased from Sigma-Aldrich (Saint Louis, USA). MS-grade trypsin/Lys-C was purchased from
Promega
(#
V5071
, Fitchburg, WI). Equipment The following equipment is required: 1st-dimension – High-pH RP-LC micro-flow
(14)
Monitoring both extended and tryptic forms of stable isotope-labeled standard peptides provides an internal quality control of proteolytic digestion in targeted mass spectrometry-based assays
Molecular & Cellular Proteomics
July 20, 2023
Rachel A. Lundeen, Jacob J. Kennedy, Oscar D. Murillo, Richard G. Ivey, Lei Zhao, Regine M. Schoenherr, Andrew N. Hoofnagle, Pei Wang, Jeffrey R. Whiteaker, Amanda G. Paulovich
experiments were obtained from
Promega
: trypsin (MS
sequencing
grade,
V5111
) and trypsin/Lys-C mix (
V5072
). A spiked internal retention time (iRT) standard set of 11 peptides was obtained from Biognosys (Ki-3002-1). Extended
(15)
Deoxyhypusine synthase mutations alter the post-translational modification of eukaryotic initiation factor 5A resulting in impaired human and mouse neural homeostasis
HGG Adv
July 13, 2023
Padgett LR, Shinkle MR, Rosario S, Stewart TM, Foley JR, Casero RA Jr, Park MH, Chung WK, Mastracci TL.
overnight
protein digestion
at 37°C (
1:70
protease:substrate ratio,
mass spectrometry
grade,
Promega
, no.
V5072
). Digestions were acidified with trifluoroacetic acid (TFA) (0.5% v/v) and desalted on Sep-Pak Vac cartridges
(16)
Proteome-wide quantitative analysis of redox cysteine availability in the Drosophila melanogaster eye reveals oxidation of phototransduction machinery during blue light exposure and age
Redox Biology
July 1, 2023
Sarah C. Stanhope, Tal Brandwine-Shemmer, Hannah R. Blum, Emma H. Doud, Amber Jannasch, Amber L. Mosley, Baruch Minke, Vikki M. Weake
proteins were digested overnight at room temperature with trypsin/LysC (ratio of
1:50
,
Promega
, Cat #
V5072
). After quenching the digestion with 0.5% TFA, samples were fractionated with a Pierce high pH basic
(17)
The protein phosphatase PPKL is a key regulator of daughter parasite development in Toxoplasma gondii
bioRxiv
June 17, 2023
Chunlin Yang, Emma H. Doud, Emily Sampson, Gustavo Arrizabalaga
phosphoproteomics,
1:70
protease: substrate ratio,
Mass Spectrometry
grade,
Promega Corporation
, Cat No:
V5072
.) Digestions were acidified with trifluoroacetic acid (TFA, 0.5% v/v) and desalted on Sep-Pak® Vac cartridges
(18)
Deep Proteomics Network and Machine Learning Analysis of Human Cerebrospinal Fluid in Japanese Encephalitis Virus Infection
Journal of Proteome Research
June 2, 2023
Tehmina Bharucha, Bevin Gangadharan, Abhinav Kumar, Ashleigh C. Myall, Nazli Ayhan, Boris Pastorino, Anisone Chanthongthip, Manivanh Vongsouvath, Mayfong Mayxay, Onanong Sengvilaipaseuth,
et al.
below 1 M. Digestion was performed with trypsin in a ratio of
1:20
m:m protein:trypsin (
Promega
, P/N
V5072
for the
pilot study
;
V5117
for the larger study); first 75% of the total amount of trypsin added and
(19)
RNA polymerase II-associated proteins reveal pathways affected in VCP-related amyotrophic lateral sclerosis
Brain
June 1, 2023
Mahmoud-Reza Rafiee, Sara Rohban, Karen Davey, Jernej Ule, Nicholas M Luscombe
neutralized by adding DTT (10 mM) again. The proteins were digested with 300 ng trypsin/LysC-mix (
Promega
,
V5071
) for 14–16 h at 37°C. Finally, the peptides were desalted using Ziptips with 0.6 µl C18 resin (Merck).
(20)
A druggable copper-signalling pathway that drives inflammation
Nature
May 1, 2023
Solier S, Müller S, Cañeque T, Versini A, Mansart A, Sindikubwabo F, Baron L, Emam L, Gestraud P, Pantoș GD,
et al
.
reach a final concentration of 1 M urea and digested overnight at 37 °C with Trypsin:Lys-C (
Promega
,
V5071
) at a ratio of
1:50
. Samples were then loaded onto a homemade C18 StageTips for
desalting
. Peptides
Page
<<
<
1
2
3
4
5
>
>>
Reviews
Be the First to Write a Review!
Trypsin/Lys-C Mix, Mass Spec Grade from Promega
Supplier Page
View Company Product Page
Product Specs
Company Info
Citations
Reviews
Back To Top