Welcome Guest
Sign In
Register
Products
Popular Categories
Antibody Search
ELISA Kit Search
Biomolecules
Flow Cytometers
Thermal Cyclers
Microscopes
Transfection Reagents
Featured Products
New Products
Articles
Resources
eBooks
Bench Tips
Tech Insights
Editorial Articles
News
Product Reviews
Future Lab
Multimedia
Biocompare Webinars
Immunotherapy
Reproducibility
Transformative Technologies
Podcasts
Tech Talks
Bench Tip Videos
All Videos and Webinars
Life Science Webinars
Write a Review
Antibodies
Kits/Reagents
Equipment
Services
Resources
Promotions
Events
AACR SPOTLIGHT
Register
Sign In
Biocompare
Products
Cell Biology
Biomolecules
Biomolecules
Micrococcal Nuclease
Micrococcal Nuclease from Cell Signaling Technology
Be the first to write a review!
Citations:
(44)
Supplier Page
Supplier Page from
Cell Signaling Technology for
Micrococcal Nuclease
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Micrococcal Nuclease
Company
Cell Signaling Technology
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
10011S
Quantity
60 µl
Applications
ChIP
Molecule Name
Micrococcal Nuclease
Research Area
Related Products
Add to Compare List
Cell Signaling Technology
3 Trask Lane
Danvers
,
Massachusetts
01923
United States
Phone
: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488
Company Profile
Website:
http://www.cellsignal.com/
Website:
Technical Support
Citations (44)
(1)
Histone deacetylase 3 regulates microglial function through histone deacetylation
Epigenetics
December 31, 2023
Laura Meleady, Morgan Towriss, Jennifer Kim, Vince Bacarac, Vivien Dang, Megan E. Rowland, Annie Vogel Ciernia
treatment condition for whole cell
chromatin
using micrococcal nuclease (
Cell Signaling Technology
#
10011
) digestion to mononucleosomes.
qPCR
of input samples was run for each qPCR primer set in serial dilutions
(2)
Low level of ARID1A contributes to adaptive immune resistance and sensitizes triple‐negative breast cancer to immune checkpoint inhibitors
Cancer Communications
September 1, 2023
Xin‐Yu Chen, Bin Li, Ye Wang, Juan Jin, Yu Yang, Lei‐Huan Huang, Meng‐Di Yang, Jian Zhang, Bi‐Yun Wang, Zhi‐Ming Shao,
et al.
approximately 1 × 107 and cross‐linked by 1% formaldehyde.
Chromatin
was digested with micrococcal nuclease (
10011
,
Cell Signaling Technology
), and the
nuclear membrane
was broken through several pulses. The DNA fragment
(3)
MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation
Nucleic acids research
August 25, 2023
Jin B, Zhu J, Pan T, Yang Y, Liang L, Zhou Y, Zhang T, Teng Y, Wang Z, Wang X,
et al
.
centrifugation
at 2000 g and digested in Buffer B containing 25 U of micrococcal nuclease (MNase) (
10011
,
CST
) per
immunoprecipitation (IP
) for 20 min at 37°C, followed by pulsed ultrasonication to shear cellular
(4)
Phosphorylation of Neurofilament Light Chain in the VLO Is Correlated with Morphine-Induced Behavioral Sensitization in Rats
International Journal of Molecular Sciences
April 22, 2023
Yu-Xiang Zhang, Yuan-Mei Zhu, Xi-Xi Yang, Fei-Fei Gao, Jie Chen, Dong-Yu Yu, Jing-Qi Gao, Zhen-Nan Chen, Jing-Si Yang, Chun-Xia Yan,
et al.
supernatants and continue with
chromatin
digestion.
Chromatin
was digested with micrococcal nuclease (#
10011
,
Cell Signaling Technology
) for 20 min at 37 °C with frequent mixing to digest DNA into a length of
(5)
Loss of Kmt2c in vivo leads to EMT, mitochondrial dysfunction and improved response to lapatinib in breast cancer
Cellular and Molecular Life Sciences
April 1, 2023
Nikiana Simigdala, Anna Chalari, Aimilia D. Sklirou, Evangelia Chavdoula, George Papafotiou, Pelagia Melissa, Aimilia Kafalidou, Nikolaos Paschalidis, Ioannis S. Pateras, Emmanouil Athanasiadis,
et al.
chromatin immunoprecipitation
as previously described [29] with an additional step of micrococcal nuclease
incubation
(
CST
#
10011
) prior to DNA
sonication
with metal probe. Antibodies: H3K4me1 (CST #
5326
), H3K4me3 (CST #
9751
), H3K27ac
(6)
The MYC-regulated lncRNA LNROP (ENSG00000254887) enables MYC-driven cell proliferation by controlling the expression of OCT2
Cell Death & Disease
February 27, 2023
Daniel García-Caballero, Jonathan R. Hart, Peter K. Vogt
fragmentation was performed enzymatically by adding 0.5 µL of
Micrococcal Nuclease
(
Cell Signaling Technology
; #
10011
) and
incubation
at 37 °C for 20 minutes to obtain the appropriate length of DNA fragments. ChIP was
(7)
PTBP2-Mediated Alternative Splicing of IRF9 Controls Tumor-Associated Monocyte/Macrophage Chemotaxis and Repolarization in Neuroblastoma Progression
Research (Washington, D.C.)
January 1, 2023
Tang J, He J, Guo H, Lin H, Li M, Yang T, Wang HY, Li D, Liu J, Li L,
et al
.
the reaction was stopped by 4 μl of 0.5 M EDTA. Total RNA was fragmented by micrococcal nuclease (#
10011
,
Cell Signaling Technology
, Beverly, USA) digestion, while the input RNA was separated by
electrophoresis
(8)
IFNγ-dependent silencing of TFF1 during Helicobacter pylori infection
Open Biology
December 14, 2022
D. Eletto, F. Mentucci, M. Vllahu, A. Voli, A. Petrella, F. Boccellato, T. F. Meyer, A. Porta, A. Tosco
were treated for nuclei extraction and DNA shearing with
Micrococcal Nuclease
(
Cell Signaling
, no.
10011
, USA) for 20 min at 37°C to obtain DNA fragments of approximately 150–900 bp. Nuclei were sonicated
(9)
Histone macroH2A1 is a stronger regulator of hippocampal transcription and memory than macroH2A2 in mice
Communications Biology
December 1, 2022
Gurdeep Singh et al.
10 s, 30 s rest between
sonications
) before digestion with micrococcal nuclease (
Cell Signaling
Cat#
10011
) for 20 min at 37 °C. Lysate was centrifuged at 17 000 RCF for 5 min at 4 °C, aliquoted and diluted
(10)
BNC1 deficiency-triggered ferroptosis through the NF2-YAP pathway induces primary ovarian insufficiency
Nature communications
October 5, 2022
Wang F, Liu Y, Ni F, Jin J, Wu Y, Huang Y, Ye X, Shen X, Ying Y, Chen J,
et al
.
Tissues were isolated into single cell suspensions using a Dounce homogenizer.
Micrococcal Nuclease
(
10011
,
Cell Signaling
) was added into each
immunoprecipitation (IP
) preparation, and the contents were mixed
(11)
MiR-585-5p impedes gastric cancer proliferation and metastasis by orchestrating the interactions among CREB1, MAPK1 and MITF
Frontiers in Immunology
October 4, 2022
Yunwei Wang, Ming Li, Jiaoxia Zeng, Yunshu Yang, Zengshan Li, Sijun Hu, Fangfang Yang, Na Wang, Wenlan Wang, Jun Tie
DDT (dithiothreitol) in line with kit instructions.
Micrococcal Nuclease
(
Cell Signaling Technology
, #
10011
) was used for digesting DNA into fragments of 150-900 bp. Following ultrasonication, the supernatants
(12)
Transcription factor Creb3l1 maintains proteostasis in neuroendocrine cells
Molecular Metabolism
September 1, 2022
Mingkwan Greenwood, Benjamin T. Gillard, Rizwan Farrukh, Alex Paterson, Ferdinand Althammer, Valery Grinevich, David Murphy, Michael P. Greenwood
resuspended in 500 μl of buffer B. To this sample 3.75 μl of
Micrococcal Nuclease
(
Cell Signaling Technology
,
10011
) was added and incubated at 37 °C for 20 min in a water
bath
to digest the DNA, mixing by inversion
(13)
Modulation of Breast Cancer Cell FASN Expression by Obesity-Related Systemic Factors
Breast Cancer: Basic and Clinical Research
August 22, 2022
Bryan McClellan, Tommy Pham, Brittany Harlow, Gabby Lee, Duan Quach, Christopher Jolly, Andrew Brenner, Linda deGraffenried
digestion with a micrococcal nuclease enzyme per manufacturer’s instructions (
Cell Signaling Technologies
, #
10011
).
Chromatin
was sheared using a VirSonic 100 ultrasonic sonicator 3× 20 seconds on ice. The resulting
(14)
Targeting HECTD3-IKKα axis inhibits inflammation-related metastasis
Signal transduction and targeted therapy
August 3, 2022
Li F, Liang H, You H, Xiao J, Xia H, Chen X, Huang M, Cheng Z, Yang C, Liu W,
et al
.
collected by centrifuge and resuspended in 100 μL cytoplasmic lysis buffer with micrococcal nuclease (
CST
, #
10011
) to digest genome DNA at 37 °C for 30 min. The pellet nuclei were collected again and resuspended in
(15)
Loss of NFE2L3 protects against inflammation-induced colorectal cancer through modulation of the tumor microenvironment
Oncogene
March 10, 2022
James Saliba et al.
Subsequently, DNA was lysed for 20 min at 37 °C using 0.4 μL of micrococcal nuclease (
Cell Signaling
,
10011
). Digestion was stopped by adding 10 μL of 0.5 mol/L EDTA (
Cell Signaling
,
7011
) per IP prep on ice
(16)
SARS-CoV-2 viroporin encoded by ORF3a triggers the NLRP3 inflammatory pathway
Virology
March 1, 2022
Xu H, Akinyemi IA, Chitre SA, Loeb JC, Lednicky JA, McIntosh MT, Bhaduri-McIntosh S.
Technology, Cat. 7007), and then treated with 0.5 μL of micrococcal nuclease (
Cell Signaling Technology
, Cat.
10011
) for 20 min at 37 °C. Nuclei were resuspended in 1 × ChIP buffer (Cell Signaling Technology, Cat. 7008)
(17)
The histone chaperone Anp32e regulates memory formation, transcription, and dendritic morphology by regulating steady-state H2A.Z binding in neurons
Cell Reports
August 17, 2021
Gilda Stefanelli et al.
400ul of TM2 buffer. Nuclei were pre warmed at 37°C for 5 min and MNase (
Cell Signaling Technologies
10011S
) was added to a final concentration of 1.25 U/ml for 10 min at 37°C. EGTA to a final concentration of
(18)
Genome-Wide Role of HSF1 in Transcriptional Regulation of Desiccation Tolerance in the Anhydrobiotic Cell Line, Pv11
International Journal of Molecular Sciences
May 28, 2021
Shoko Tokumoto et al.
chromatin
digestion. To digest DNA to a length of approximately 150–300 bp, 2 µL micrococcal nuclease (Cat#
10011
by
Cell Signaling Technology
) was added and the samples were incubated for 20 min at 37 ◦ C. After terminating
(19)
Genome-Wide Chromatin Analysis of FFPE Tissues Using a Dual-Arm Robot with Clinical Potential
Cancers (Basel)
April 28, 2021
Syuzo Kaneko et al.
100 µL of Buffer B with 0.5 mM DTT. Then, 1 µL of micrococcal nuclease (
Cell Signaling Technology
, #
10011
) was added into the nuclei mixture and incubated for 20 min at 37 ◦ C with frequent mixing (800 rpm).
(20)
M-Cadherin Is a PAX3 Target During Myotome Patterning
Frontiers in Cell and Developmental Biology
April 1, 2021
Joana Esteves de Lima, Reem Bou Akar, Myriam Mansour, Didier Rocancourt, Margaret Buckingham, Frédéric Relaix
incubated for 15 min on ice.
Chromatin
digestion was performed with
Micrococcal nuclease
(
Cell Signaling
, #
10011
) for 40 min at 37°C. The supernatant containing 20 μg of DNA was incubated with 20 μL of magnetic beads
Page
<<
<
1
2
3
>
>>
Reviews
Be the First to Write a Review!
Micrococcal Nuclease from Cell Signaling Technology
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
Back To Top