Description
Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.
Quality
Mouse colonies are routinely screened for pathogens via Mouse Antibody Production (MAP) testing
Extensive PCR testing is performed to screen for a number of pathogens, including LDEV, to ensure strict control of raw materials used during the manufacturing process
Tested and found negative for bacteria, fungi, and mycoplasma
Protein concentrations are determined by Lowry method
Endotoxin units are measured by Limulus Amoebocyte Lysate assay
Corning Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
Biological activity is determined for each lot using a neurite outgrowth assay. Chick dorsal root ganglia are plated on a 1.0mm layer of Corning Matrigel Matrix and must generate positive neurite outgrowth response after 48 hours without addition of nerve growth factor
Source
Engelbreth-Holm-Swarm Mouse Tumor
Preparation and Storage
Store at -20°C. Avoid multiple freeze-thaws. Do not store in frost-free freezer. KEEP FROZEN.
Application
Cell Growth and Differentiation
Metabolism/Toxicology Studies
Invasion Assays
in vitro and in vivo Angiogenesis Assays
in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice