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PCR Master Mixes (High Fidelity)
Q5 High-Fidelity 2X Master Mix
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Q5 High-Fidelity 2X Master Mix
Q5 High-Fidelity 2X Master Mix from New England Biolabs
(1)
Write a review
Citations:
(304)
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Supplier Page from
New England Biolabs for
Q5 High-Fidelity 2X Master Mix
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Q5 High-Fidelity 2X Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0492S
Quantity
100 reactions
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (304)
(101)
Horizontal gene transfer enables programmable gene stability in synthetic microbiota
Nature Chemical Biology
November 1, 2022
Teng Wang, Andrea Weiss, Ammara Aqeel, Feilun Wu, Allison J. Lopatkin, Lawrence A. David, Lingchong You
(Supplementary Table 4, Primers 1 and 2,
Q5® High-Fidelity 2X Master Mix
,
New England Biolabs
, Ref#:
M0492
)). Gibson assembly
cloning
was used to introduce a synthetic barcode sequence (synthesized by an external
(102)
Small NRPS-like enzymes in Aspergillus sections Flavi and Circumdati selectively form substituted pyrazinone metabolites
Frontiers in Fungal Biology
October 26, 2022
Matthew D. Lebar, Brian M. Mack, Carol H. Carter-Wientjes, Qijian Wei, Christopher P. Mattison, Jeffrey W. Cary
(AFLA_023020) native promotor and terminator were amplified by
PCR
using
Q5 High-Fidelity DNA Polymerase
(
NEB
M0492S
). A. sclerotiorum gDNA was the template for
amplification
of the NRPS of asaC_AS using the primer pair
(103)
Human NOP2/NSUN1 regulates ribosome biogenesis through non-catalytic complex formation with box C/D snoRNPs
Nucleic Acids Research
October 14, 2022
Han Liao, Anushri Gaur, Hunter McConie, Amirtha Shekar, Karen Wang, Jeffrey T Chang, Ghislain Breton, Catherine Denicourt
Protein G Dynabeads (
10003D
,
Invitrogen
), Protein G agarose beads (
101242
,
Invitrogen
), Q5 2X master mix (
M0492S
,
NEB
),
Q5 Site-Directed Mutagenesis Kit
(E0554, NEB), RevertAid reverse transcriptase (
EP0441
,
Thermo Scientific
(104)
Genetic Diversity and Evolutionary Convergence of Cryptic SARS-CoV-2 Lineages Detected Via Wastewater Sequencing
medRxiv
September 23, 2022
Devon A. Gregory et al.
ul of lysed template was added to the following PCR mix: 10 ul
Q5 High-Fidelity 2X Master Mix
(
NEB
#
M0492S
) + 0.8 ul 5uM CATTCAACTCAGGACTTGTTCTT forward primer (2402F) + 0.8 ul 5uM ATGTCAAGAATCTCAAGTGTCTG (2376R)
(105)
Sensitive and reproducible cell-free methylome quantification with synthetic spike-in controls
Cell Reports Methods
September 19, 2022
Samantha L. Wilson, Shu Yi Shen, Lauren Harmon, Justin M. Burgener, Tim Triche, Scott V. Bratman, Daniel D. De Carvalho, Michael M. Hoffman
fragments by
PCR
. We used the High-Fidelity 2X Master Mix (
New England Biolabs
, Ipswich, MA, USA, Cat #
M0492L
) and the fragments’ optimal annealing temperatures (Table S1). We purified amplified fragments using
(106)
Protocol to study sufficiency of cis-regulatory elements in mouse embryonic stem cells using a CRISPR-mediated knockin approach
STAR Protocols
September 16, 2022
Tomas Pachano, Alvaro Rada-Iglesias
PureYield Plasmid Miniprep System
,
Promega
,Cat:
A1222
Q5® High-Fidelity 2× Master Mix,
New England Biolabs
,Cat:
M0492L
GoTaq polymerase,
Promega
,Cat:
M7123
PureLink™ Genomic DNA Mini Kit
,
Thermo Fisher Scientific
,Cat:
K182001
,,
Experimental models
(107)
Genomic epidemiology identifies emergence and rapid transmission of SARS-CoV-2 B.1.1.7 in the United States
medRxiv
September 14, 2022
Nicole L. Washington et al.
using ARTIC Network n-CoV-19 V3 primers and Q5 DNA High-Fidelity Master Mix (
New England BioLabs
, #
M0492L
) to generate tiled
PCR
amplicons. Libraries were then prepared for
sequencing
on Illumina platforms
(108)
Multifaceted role of RNA editing in promoting loss-of-function of PODXL in cancer
iScience
August 19, 2022
Ting Fu, Tracey W. Chan, Jae Hoon Bahn, Tae-Hyung Kim, Amy C. Rowat, Xinshu Xiao
PowerUp™ SYBR® Green Master Mix
,
Thermo Fisher Scientific
,Cat#
A25742
Q5® Hot Start High-Fidelity 2X Master Mix
,
NEB
,Cat#
M0492L
"
Pierce™ Protease Inhibitor Tablets, EDTA-free
",
Thermo Fisher Scientific
,Cat# A32965 "
PageRuler™ Prestained Protein Ladder, 10 to 180 kDa
(109)
A suite of PCR-LwCas13a assays for detection and genotyping of Treponema pallidum in clinical samples
Nature Communications
August 9, 2022
Wentao Chen et al.
23 S rRNA) were amplified by using Q5 High-Fidelity DNA Polymerases (
New England Biolabs
, MA, USA;
M0492S
). A total of 25 μL reaction volume included 1.25 μL of 10 μM tpp47-dsDNA or tp0548-dsDNA or 23 S rRNA-dsDNA
(110)
Serial H3K27M cell-free tumor DNA (cf-tDNA) tracking predicts ONC201 treatment response and progression in diffuse midline glioma
Neuro-Oncology
August 1, 2022
Evan Cantor et al.
using the same primers described above and the
Q5 High-Fidelity 2X Master Mix
(
New England BioLabs
,
M0492S
). The following
PCR
protocol was used: 1 cycle at 98 °C for 30 s, 12 cycles at 98 °C for 10 s, 58 °C
(111)
Machine Learning Guided Batched Design of a Bacterial Ribosome Binding Site
ACS Synthetic Biology
July 15, 2022
Mengyan Zhang, Maciej Bartosz Holowko, Huw Hayman Zumpe, Cheng Soon Ong
the
cloning
inserts was done using
Q5 High-Fidelity 2X Master Mix
(
NEB
, Ipswich, MA, catalogue no.
M0492L
). Twenty μL reactions were prepared by dispensing 1 μL of each 10 μM reverse primer into the wells of
(112)
Compatibility rules of human enhancer and promoter sequences
Nature
July 7, 2022
Drew T. Bergman, Thouis R. Jones, Vincent Liu, Judhajeet Ray, Evelyn Jagoda, Layla Siraj, Helen Y. Kang, Joseph Nasser, Michael Kane, Antonio Rios,
et al.
amplified enhancers and promoters separately from the same array using
Q5 high-fidelity DNA polymerase
(
NEB
M0492
) (see Supplementary Table 10 for primer sequences). We amplified enhancers in four 50uL
PCR reactions
(113)
Germinal center activity and B cell maturation are associated with protective antibody responses against Plasmodium pre-erythrocytic infection
PLOS Pathogens
July 6, 2022
Ganesh Ram R. Visweswaran et al.
Subsequent
amplification
was performed with primers vv-869, vv-870 and vv-872, using Q5 2x Master Mix (
NEB
, #
M0492S
). The reaction was incubated for 30 s at 98°C, then cycled 18 times at 98°C for 10 s, 63°C for 30 s,
(114)
Cell cycle arrest explains the observed bulk 3D genomic alterations in response to long-term heat shock in K562 cells
Genome Research
July 1, 2022
Bingxiang Xu, Xiaomeng Gao, Xiaoli Li, Yan Jia, Feifei Li, Zhihua Zhang
65602
). Finally, the Hi-C library was amplified for about 10 cycles of
PCR
with the Q5 master mix (
NEB
M0492L
), following the manufacturer's instructions. Size selection was performed with AMPure XP beads, quantified,
(115)
CryoEM structures of pseudouridine-free ribosome suggest impacts of chemical modifications on ribosome conformations
Structure
July 1, 2022
Yu Zhao, Jay Rai, Hongguo Yu, Hong Li
assays
E.Z.N.A. Plasmid DNA Miniprep Kit,Omega BIO-TEK,D6942-01
Q5® High-Fidelity 2X Master Mix
,
NEB
,
M0492S
"
Zero Blunt™ TOPO™ PCR Cloning Kit, without competent cells
",
ThermoFisher
,
450245
Deposited data,Deposited
(116)
An optimized FusX assembly-based technique to introduce mitochondrial TC-to-TT variations in human cell lines
STAR Protocols
June 17, 2022
Bibekananda Kar, Ankit Sabharwal, Santiago Restrepo-Castillo, Brandon W. Simone, Karl J. Clark, Stephen C. Ekker
Polymerase,Meridian Bioscience,Cat# BIO-21108 Q5 High-Fidelity DNA Polymerases,
New England BioLabs
,Cat#
M0492S
,, Critical commercial
assays
,Critical commercial
assays
,Critical commercial
assays
,, QIAprep Spin
(117)
Efficient suppression of endogenous CFTR nonsense mutations using anticodon-engineered transfer RNAs
Molecular Therapy - Nucleic Acids
June 14, 2022
Wooree Ko, Joseph J. Porter, Matthew T. Sipple, Katherine M. Edwards, John D. Lueck
were obtained from New England Biolabs. For all
PCR reactions
,
Q5 High-Fidelity 2X Master Mix
(
NEB
M0492L
) was used, Gibson assembly was performed using
NEBuilder HiFi DNA Assembly Master Mix
(
NEB
E2621L
),
(118)
Immunogenicity of convalescent and vaccinated sera against clinical isolates of ancestral SARS-CoV-2, Beta, Delta, and Omicron variants
Med (N Y)
June 10, 2022
Arinjay Banerjee et al.
IDT,https://github.com/artic-network/artic-ncov2019
Q5 High-Fidelity 2x Master Mix
,
New England Biolabs
,
M0492L
,, Deposited data,Deposited data,Deposited data ,, Raw data and scripts,This study,https://github.com/fmaguire/voc_neutralisation_sc2_phylogenomics Code,This
(119)
Hypoxia-directed tumor targeting of CRISPR-Cas9 and HSV-TK suicide gene therapy using lipid nanoparticles
Molecular Therapy - Methods & Clinical Development
June 9, 2022
Alicia Davis, Kevin V. Morris, Galina Shevchenko
Maxwell RSC Cultured Cells DNA Kit
(
Promega
AS1620
). DNA was amplified using
Q5 High-Fidelity 2X Master Mix
(
New England BioLabs
M0492L
) from 100 ng genomic DNA according to the protocol provided by the manufacturer. The following primers
(120)
RNF43 G659fs is an oncogenic colorectal cancer mutation and sensitizes tumor cells to PI3K/mTOR inhibition
Nature communications
June 8, 2022
Fang L, Ford-Roshon D, Russo M, O'Brien C, Xiong X, Gurjao C, Grandclaudon M, Raghavan S, Corsello SM, Carr SA,
et al
.
an amplicon roughly 200–300 bp in length using
Q5 High-Fidelity 2X Master Mix
(
New England Biolabs
, #
M0492S
) and confirmed by
next generation sequencing
to determine editing efficiency (Massachusetts General
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Reviews
Write a Review
(1)
8/6/2019
PCR Master Mix for Superior Performance for a Broad Range of Amplicons (NEB)
Atul Singh
It works well for all kinds of PCR.
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Q5 High-Fidelity 2X Master Mix from New England Biolabs
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