Welcome Guest
Sign In
Register
Products
Popular Categories
Antibody Search
ELISA Kit Search
Biomolecules
Flow Cytometers
Thermal Cyclers
Microscopes
Transfection Reagents
Featured Products
New Products
Articles
Resources
eBooks
Bench Tips
Tech Insights
Editorial Articles
News
Product Reviews
Future Lab
Multimedia
Biocompare Webinars
Immunotherapy
Reproducibility
Transformative Technologies
Podcasts
Tech Talks
Bench Tip Videos
All Videos and Webinars
Life Science Webinars
Write a Review
Antibodies
Kits/Reagents
Equipment
Services
Resources
Promotions
Events
AACR SPOTLIGHT
Register
Sign In
Biocompare
Products
PCR Master Mixes (High Fidelity)
Q5 High-Fidelity 2X Master Mix
Supplier Images
Q5 High-Fidelity 2X Master Mix
Q5 High-Fidelity 2X Master Mix from New England Biolabs
(1)
Write a review
Citations:
(304)
Supplier Page
Supplier Page from
New England Biolabs for
Q5 High-Fidelity 2X Master Mix
Get Pricing
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Q5 High-Fidelity 2X Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0492S
Quantity
100 reactions
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (304)
(41)
phox2ba: The Potential Genetic Link behind the Overlap in the Symptomatology between CHARGE and Central Congenital Hypoventilation Syndromes
Genes (Basel)
May 15, 2023
Jessica E. MacLean, Jaime N. Wertman, Sergey V. Prykhozhij, Emily Chedrawe, Stewart Langley, Shelby L. Steele, Kevin Ban, Kim Blake, Jason N. Berman
phox2ba_BamHI_linker_rev (GATCGGATCCACTGCCTCCACCGCCACACAGGCACACAGAATCAA) from cDNA using
Q5 High-Fidelity 2X Master Mix
(
M0492S
;
New England Biolabs
Inc., Ipswich, MA, USA) using the standard Q5 protocol with 65 °C annealing temperature.
(42)
Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome
Cell
May 11, 2023
Healy MD, McNally KE, Butkovič R, Chilton M, Kato K, Sacharz J, McConville C, Moody ERR, Shaw S, Planelles-Herrero VJ,
et al
.
EGFP-N1 or lentiviral pLVX vector.
VPS35L
was amplified using
Q5 High-Fidelity 2X Master Mix
(
NEB
,
M0492
) following the manufacturer’s protocol. Following
PCR
, bands were resolved on agarose gel and purified
(43)
Single-cell RNA-seq uncovers dynamic processes orchestrated by RNA-binding protein DDX43 in chromatin remodeling during spermiogenesis
Nature communications
April 29, 2023
Tan H, Wang W, Zhou C, Wang Y, Zhang S, Yang P, Guo R, Chen W, Zhang J, Ye L,
et al
.
remove unincorporated primer, and further ligated with 3′ DNA adaptor. Finally, we used Q5 PCR mix (
NEB
,
M0492L
) to amplify the libraries for
high-throughput sequencing
in Illumina platform.
RNA-seq
libraries We
(44)
Engineering Nme2Cas9 Adenine Base Editors with Improved Activity and Targeting Scope
bioRxiv
April 23, 2023
Nathan Bamidele, Han Zhang, Xiaolong Dong, Nicholas Gaston, Haoyang Cheng, Karen Kelly, Jonathan K. Watts, Jun Xie, Guangping Gao, Erik J. Sontheimer
preparation, and analysis were performed as previously described12. Briefly, Q5 High-Fidelity polymerase (
NEB
#
M0492
) was used to amplify genomic DNA for library preparation, and libraries were pooled and purified twice
(45)
Maximal interferon induction by influenza lacking NS1 is infrequent owing to requirements for replication and export
PLOS Pathogens
April 17, 2023
Alison C. Vicary, Marisa Mendes, Sharmada Swaminath, Asama Lekbua, Jack Reddan, Zaida K. Rodriguez, Alistair B. Russell
Hoffmann et al. (2001) for 16 cycles using Q5 polymerase master mix from
New England Biolabs
(catalog
M0492
). Resulting cDNA was then tagmented using the Nextera DNA Flex LPK kit (catolog 20018704) and indexed
(46)
High-throughput screening of spike variants uncovers the key residues that alter the affinity and antigenicity of SARS-CoV-2
Cell Discovery
April 11, 2023
Yufeng Luo, Shuo Liu, Jiguo Xue, Ye Yang, Junxuan Zhao, Ying Sun, Bolun Wang, Shenyi Yin, Juan Li, Yuchao Xia,
et al.
variants were obtained by
site-directed mutagenesis
primers using 2× Q5 Hi-fidelity mix (
NEB
, Cat No.:
M0492
). For
protein expression
, pCDNA3.1(+) was first cut with
BamHI
(
NEB
, Cat No.:
R3136
) and
XhoI
(
NEB
,
(47)
Polycomb response elements reduce leaky expression of Cas9 under temperature-inducible Hsp70Bb promoter in Drosophila melanogaster
G3 Genes|Genomes|Genetics
April 11, 2023
Natalie Warsinger-Pepe, Carly Chang, Connor R Desroberts, Omar S Akbari
Hsp70Bb-Cas9-T2A-eGFP (Kandul et al. 2021) was
PCR
amplified with
NEB
Q5 High-Fidelity 2X Master Mix
(
M0492
) using primers 1157_onestep_p1 and 1157_onestep_p2. PRExpress (Akmammedov et al. 2017) was obtained
(48)
GINS4 suppresses ferroptosis by antagonizing p53 acetylation with Snail
Proceedings of the National Academy of Sciences
April 11, 2023
Ling Chen, Qidong Cai, Rui Yang, Haiyan Wang, Huli Ling, Tiansheng Li, Na Liu, Zuli Wang, Jingyue Sun, Tania Tao,
et al.
expressing pEGFP-C3-
p53
with point mutation(s) was generated by using Q5 ® Ultra-Fidelity 2X premix (#
M0492S
,
NEB
) and QuickCut™ Dpn I (1609, TAKARA) according to the manufacturer’s protocol.
CRISPR/Cas9
-Mediated
(49)
The US9-Derived Protein gPTB9TM Modulates APP Processing Without Targeting Secretase Activities
Molecular neurobiology
April 1, 2023
Brandimarti R, Irollo E, Meucci O.
Construction Inserts in g9A10pep and gPTB9TM were amplified with Q5 polymerase from
New England Biolabs
(
M0492S
) and assembled using the Gibson assembly strategy (NEBuilder® HiFi DNA Assembly Kit,
E5520S
). Oligonucleotides
(50)
Continuous fish muscle cell line with capacity for myogenic and adipogenic-like phenotypes
Scientific Reports
March 29, 2023
Michael K. Saad, John S. K. Yuen, Connor M. Joyce, Xinxin Li, Taehwan Lim, Talia L. Wolfson, Justin Wu, Jason Laird, Sanjana Vissapragada, Olivia P. Calkins,
et al.
from Aranishi19,
PCR
was performed with
Q5® High-Fidelity 2X Master Mix
(
New England Biolabs
(
NEB
) #
M0492
, Ipswich, MA, USA), with primers designed to target fish universal 5S rDNA (5S primers) and Scomber
(51)
A cell-type-specific alternative splicing regulator shapes synapse properties in a trans-synaptic manner
Cell reports
March 28, 2023
Traunmüller L, Schulz J, Ortiz R, Feng H, Furlanis E, Gomez AM, Schreiner D, Bischofberger J, Zhang C, Scheiffele P.
"
T4 RNA Ligase 1 (ssRNA Ligase), High Concentration
",
NEB
,#
M0437M
"
Proteinase K, Molecular Biology Grade
(0.8 U/ul)",
NEB
,#
P8107S
Q5® High-Fidelity 2X Master Mix
,
NEB
,#
M0492S
ExoSAP-IT™,Applied Biosystems,#78200.200.UL
SuperScript™ III Reverse Transcriptase
,
Invitrogen
,#
18080093
Dynabeads™ MyOne™ Silane
(52)
Self-delivering CRISPR RNAs for AAV Co-delivery and Genome Editing in vivo
bioRxiv
March 28, 2023
Han Zhang, Karen Kelly, Jonathan Lee, Dimas Echeverria, David Cooper, Rebecca Panwala, Zexiang Chen, Nicholas Gaston, Gregory A. Newby, Jun Xie,
et al.
deep sequencing
and data analysis. Genomic DNA was
PCR
-amplified using
Q5 High-Fidelity 2x Master Mix
(
NEB
M0492
) for 20 cycles. For barcoding
PCR
, 1 μl of the unpurified
PCR product
was used as a template and amplified
(53)
Addressing nontarget amplification in DNA metabarcoding studies of arthropod-feeding rodents
Mammal Research
March 27, 2023
Dylan M. Klure, Robert Greenhalgh, M. Denise Dearing
consisted of a total volume of 25 μl: 12.5 μl of High-Fidelity 2X Master Mix (
New England Biolabs
#
M0492S
), 1.5 μl of each primer LCO1490/COI-CFMRa, 0.5 μl of 25 mM MgCl2, 4 μl of nuclease-free molecular biology
(54)
CDetection.v2: One-pot assay for the detection of SARS-CoV-2
Frontiers in Microbiology
March 23, 2023
Xinge Wang, Yangcan Chen, Xuejia Cheng, Si-Qi Wang, Yanping Hu, Yingmei Feng, Ronghua Jin, Kangping Zhou, Ti Liu, Jianxing Wang,
et al.
refining the product obtained from
PCR
with
Q5® High-Fidelity 2X Master Mix
(
NEB
, Ipswich, England,
M0492S
). The concentration of activator is determined by Nanodrop, and this is subsequently used to calculate
(55)
Revealing determinants of translation efficiency via whole-gene codon randomization and machine learning
Nucleic Acids Research
March 21, 2023
Thijs Nieuwkoop, Barbara R Terlouw, Katherine G Stevens, Richard A Scheltema, Dick de Ridder, John van der Oost, Nico J Claassens
cultures, 1 μl of cells were used in a
PCR
reaction to amplify the DNA for
Sanger sequencing
using Q5 (
NEB
,
M0492
). The
PCR
reaction was sent to Macrogen Europe B.V. for sample
clean-up
and
Sanger sequencing
. All
cell cultures
(56)
Transcriptome Analysis of Diffuse Large B-Cell Lymphoma Cells Inducibly Expressing MyD88 L265P Mutation Identifies Upregulated CD44, LGALS3, NFKBIZ, and BATF as Downstream Targets of Oncogenic NF-κB Signaling
International Journal of Molecular Sciences
March 15, 2023
Marcello Turi, Anjana Anilkumar Sithara, Lucie Hofmanová, David Žihala, Dhwani Radhakrishnan, Alexander Vdovin, Sofija Knápková, Tereza Ševčíková, Zuzana Chyra, Tomáš Jelínek,
et al.
instructions. The sequences were amplified with Q5 High-Fidelity 2× Master Mix (
NEB
, Ipswich, MA, USA; cat. No.
M0492S
) using a set of primers for introducing the restriction sites for
EcoRI
and
AgeI
, at 5′ and 3′, respectively
(57)
Assessment of microbiota in the gut and upper respiratory tract associated with SARS-CoV-2 infection
Microbiome
March 3, 2023
Jiarui Li, Qiuyu Jing, Jie Li, Mingxi Hua, Lin Di, Chuan Song, Yanyi Huang, Jianbin Wang, Chen Chen, Angela Ruohao Wu
min. A 20-μl tagmentation product was mixed with 20.4 μl
Q5 High-Fidelity 2X Master Mix
(
NEB
, Cat.No.
M0492L
), 0.4 μl SuperScript II reverse transcriptase, and incubated at 42°C for 15 min to fill the gaps, followed
(58)
An efficient and cost-effective purification protocol for Staphylococcus aureus Cas9 nuclease
STAR Protocols
March 1, 2023
Allen C.T. Teng, Marjan Tavassoli, Suja Shrestha, Ryan M. Marks, Meghan J. McFadden, Sonia L. Evagelou, Kyle Lindsay, Ava Vandenbelt, Wenping Li, Evgueni Ivakine,
et al.
SLGPR33RS QIA prep Spin Miniprep Kit,
Qiagen
,Cat#
27104
Q5 High-Fidelity 2× Master Mix,
New England Biolabs
,Cat#
M0492S
QIA quick PCR purification kit,
Qiagen
,Cat#
28104
MEGAshortscript T7 transcription kit
,
Thermo Fisher Scientific
(59)
Structural snapshots of R-loop formation by a type I-C CRISPR Cascade
Molecular Cell
March 1, 2023
Roisin E. O’Brien, Jack P.K. Bravo, Delisa Ramos, Grace N. Hibshman, Jacquelyn T. Wright, David W. Taylor
Q5 Site-Directed Mutagenesis Kit
,
NEB
,
E0554S
Gibson Assembly Cloning Kit
,
NEB
,
E5510S
Q5 High Fidelity 2X Master MIx
,
NEB
,
M0492S
Q1Aprep Spin Miniprep Kit,
Qiagen
,
27104
,, Deposited Data,Deposited Data,Deposited Data ,, Type
(60)
Coordination of bacterial cell wall and outer membrane biosynthesis
Nature
March 1, 2023
Hummels KR, Berry SP, Li Z, Taguchi A, Min JK, Walker S, Marks DS, Bernhardt TG.
4. Unless stated otherwise,
polymerase chain reaction (PCR
) was carried out using Q5 polymerase (
NEB
M0492L
) for
cloning
purposes and GoTaq Green DNA polymerase (
Promega
M7123
) for diagnostic purposes, both according
Page
<<
<
1
2
3
4
5
>
>>
Reviews
Write a Review
(1)
8/6/2019
PCR Master Mix for Superior Performance for a Broad Range of Amplicons (NEB)
Atul Singh
It works well for all kinds of PCR.
<<
<
>
>>
Q5 High-Fidelity 2X Master Mix from New England Biolabs
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
Back To Top