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PCR Master Mixes (High Fidelity)
Q5 High-Fidelity 2X Master Mix
Supplier Images
Q5 High-Fidelity 2X Master Mix
Q5 High-Fidelity 2X Master Mix from New England Biolabs
(1)
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Citations:
(302)
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Supplier Page from
New England Biolabs for
Q5 High-Fidelity 2X Master Mix
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Q5 High-Fidelity 2X Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0492S
Quantity
100 reactions
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (302)
(21)
mRNA Delivery Platform Based on Bacterial Outer Membrane Vesicles for Tumor Vaccine
Bio-protocol
July 5, 2023
Gao X, Li Y, Nie G, Zhao X.
(
New England Biolabs
, catalog number:
E2060S
)Q5 high-fidelity 2× master mix (
New England Biolabs
, catalog number:
M0492S
)Tris-HCl (1 M, pH 8.0) (Solarbio, catalog number: T1150)MgCl2 (1 M) (Beyotime, catalog number: ST269)Diethylpyrocarbonate
(22)
Biochemical and structural characterization of the first-discovered metazoan DNA cytosine-N4 methyltransferase from the Bdelloid rotifer Adineta vaga
Journal of Biological Chemistry
July 5, 2023
Jujun Zhou, John R. Horton, Gundeep Kaur, Qin Chen, Xuwen Li, Fabian Mendoza, Tao Wu, Robert M. Blumenthal, Xing Zhang, Xiaodong Cheng
Multiplex
Oligos for Illumina (
NEB
,
E7335S
/
E7500S
/
E7710S
/
E7730S
) and
Q5 High-Fidelity 2X Master Mix
(
NEB
,
M0492S
) (cycle number was determined by
qPCR
with
PowerUP SYBR Green Master Mix
using R1-22nt (5′-CTA CAC GAC
(23)
Assessment of enzymatically synthesized DNA for gene assembly
Frontiers in Bioengineering and Biotechnology
July 5, 2023
Brooke L. Simmons, Nathan D. McDonald, Natalie G. Robinett
incubated at 50°C/30 min. Next, 5 µL of this mixture was added to 12.5 µL of ×2 Q5 Master Mix (
NEB
, #
M0492S
), 2.5 µL each end oligo, and H2O to reach a final concentration of 25 µL. The
PCR
cycles were run as
(24)
Aclarubicin stimulates RNA polymerase II elongation at closely spaced divergent promoters
Science Advances
June 16, 2023
Matthew Wooten, Brittany Takushi, Kami Ahmad, Steven Henikoff
and a uniquely barcoded i7 primer and amplified with
NEB
Q5 high-fidelity 2× master mix (catalog no.
M0492S
). The libraries were purified with 1.1× volume of Sera-Mag carboxylate-modified magnetic beads and subjected
(25)
Modularity and diversity of target selectors in Tn7 transposons
Molecular Cell
June 15, 2023
Guilhem Faure, Makoto Saito, Sean Benler, Iris Peng, Yuri I. Wolf, Jonathan Strecker, Han Altae-Tran, Edwin Neumann, David Li, Kira S. Makarova,
et al.
NEBNext® High-Fidelity 2X PCR Master Mix
,
New England Biolabs
,
M0541
Q5® High-Fidelity 2X Master Mix
,
New England Biolabs
,
M0492
KAPA HiFi HotStart ReadyMix
,
Roche
,KK2602
KOD Hot Start DNA Polymerase
,
Millipore Sigma
,
71086-3
"
E-Gel™ EX Agarose Gels, 1
(26)
Reduction of Tumor Growth with RNA-Targeting Treatment of the NAB2–STAT6 Fusion Transcript in Solitary Fibrous Tumor Models
Cancers (Basel)
June 9, 2023
Yi Li, John T. Nguyen, Manasvini Ammanamanchi, Zikun Zhou, Elijah F. Harbut, Jose L. Mondaza-Hernandez, Clark A. Meyer, David S. Moura, Javier Martin-Broto, Heather N. Hayenga,
et al.
performed using Q5 High-Fidelity 2× Master Mix (
New England Biolabs
, Ipswich, WA, USA, catalog number:
M0492
). An amount of 100 ng genomic DNAs were used as the template, and the
PCR
conditions were: first, 1
(27)
Muscleblind-like proteins use modular domains to localize RNAs by riding kinesins and docking to membranes
Nature communications
June 9, 2023
Hildebrandt RP, Moss KR, Janusz-Kaminska A, Knudson LA, Denes LT, Saxena T, Boggupalli DP, Li Z, Lin K, Bassell GJ,
et al
.
(cytosolic), and Calnexin (
membrane
) using
qPCR
primers (IDT) with Q5 polymerase (
New England Biolabs
,
M0492
) was measured with a highly sensitive dsDNA detection dye (Lumiprobe, 11010) on a 96-well
RT-PCR
thermal
(28)
Group B Streptococcus Cas9 variants provide insight into programmable gene repression and CRISPR-Cas transcriptional effects
Communications Biology
June 9, 2023
Kathyayini P. Gopalakrishna, Gideon H. Hillebrand, Venkata H. Bhavana, Jordan L. Elder, Adonis D’Mello, Hervé Tettelin, Thomas A. Hooven
ensure silent mutations.
Mutagenesis
PCR
was performed using
NEB
Q5 High Fidelity Polymerase (cat. #
M0492L
).
PCR
products were digested with 1 μL of
DpnI
for 1 h at 37 °C. Samples were then Gibson assembled
(29)
Evaluating the function of murine quiescent hematopoietic stem cells following non-homologous end joining-based genome editing
STAR Protocols
June 9, 2023
Kohei Shiroshita, Hiroshi Kobayashi, Keiyo Takubo
314-08691 TaKaRa Ex Taq,Takara Bio Inc,Cat# RR001A
Q5 High-Fidelity 2x Master Mix
,
New England Biolabs
,Cat#
M0492S
Wizard SV Gel and PCR Clean-Up System
,
Promega
,Cat#
A9282
,,
Experimental models
: Organisms/
strains
,
Experimental models
(30)
Pathogen-specific innate immune response patterns are distinctly affected by genetic diversity
Nature Communications
June 5, 2023
Antje Häder, Sascha Schäuble, Jan Gehlen, Nadja Thielemann, Benedikt C. Buerfent, Vitalia Schüller, Timo Hess, Thomas Wolf, Julia Schröder, Michael Weber,
et al.
ATCATTGAAATTAATTTCACCTGTCCA) with 2x Q5 polymerase master mix according to the manufacturer’s instructions (
NEB
Cat#
M0492L
).
PCR
samples were purified with the
PCR
& Gel
Clean-Up
Kit (Macherey-Nagel Cat# 740609.50) according
(31)
Non-viral nitric oxide-based gene therapy improves perfusion and liposomal doxorubicin sonopermeation in neuroblastoma models
Theranostics
June 4, 2023
Aditi Bellary, Chance Nowak, Isabella Iwanicki, Fernando Flores-Guzman, Lydia Wu, Jessica J. Kandel, Theodore W. Laetsch, Leonidas Bleris, Sonia L. Hernandez, Shashank R. Sirsi
plasmid #19295) 43. The transcript was cloned using
PCR
with
Q5® High-Fidelity DNA Polymerase
(
NEB
, #
M0492S
) and primers P1: cagtagaccggtgagactctggccccacgggacacag and P2: cagtagcaattggaattgtaatacgactcactatagg.
(32)
Protocol for biallelic tagging of an endogenous gene using CRISPR-Cas9 in human cells
STAR Protocols
June 1, 2023
Nannan Kong, Ying Wai Chan
6023 DNeasy Blood & Tissue Kits,
Qiagen
,Cat#
69504
Q5 High-Fidelity 2× Master Mix,
New England Biolabs
,Cat#
M0492L
FastDigest
Bpil,
Thermo Fisher
,Cat#
FD1014
T7 DNA ligase
,
New England Biolabs
,Cat#
M0318S
BamHI-HF
,
New England Biolabs
(33)
scChIX-seq infers dynamic relationships between histone modifications in single cells
Nature Biotechnology
June 1, 2023
Jake Yeung, Maria Florescu, Peter Zeller, Buys Anton de Barbanson, Max D. Wellenstein, Alexander van Oudenaarden
barcodes and handles was performed by adding 25 μl of
NEBNext Ultra II Q5 Master Mix
(
NEB
, catalog no.
M0492L
), 11 μl nuclease-free water and 2 μl of 10 μM RP1 and RPIx primers.
PCR
protocol for sortChIC experiments Activation
(34)
Identification and heterologous expression of the globomycin biosynthetic gene cluster
Synthetic and Systems Biotechnology
June 1, 2023
Daniel Oves-Costales, Tetiana Gren, Eva Baggesgaard Sterndorff, Jesús Martín, Francisco Javier Ortiz-López, Tue S. Jørgensen, Xinglin Jiang, Fernando Román-Hurtado, Fernando Reyes, Olga Genilloud,
et al.
pL0004. The
PCR
reaction was carried out with the Q5 High Fidelity Master Mix (
New England Biolabs
;
M0492S
), following the manufacturer's instructions. The
spores
for
colony PCR
were harvested from plates using
(35)
A noncommutative combinatorial protein logic circuit controls cell orientation in nanoenvironments
Science Advances
May 26, 2023
Jiaxing Chen, Yashavantha L. Vishweshwaraiah, Richard B. Mailman, Erdem D. Tabdanov, Nikolay V. Dokholyan
LOV2. We performed all PCRs using Q5 High-Fidelity Master Mix [New England Biolabs (
NEB
), cat. no.
M0492S
]. After the reaction, we added
DpnI
(
NEB
, cat. no,
R0176S
) enzyme into the
PCR
mixture to digest the
(36)
Reconstruction of a polyclonal ADCC antibody repertoire from an HIV-1 non-transmitting mother
iScience
May 19, 2023
Zak A. Yaffe, Shilei Ding, Kevin Sung, Vrasha Chohan, Lorie Marchitto, Laura Doepker, Duncan Ralph, Ruth Nduati, Frederick A. Matsen, Andrés Finzi,
et al.
proteins","Chemicals, peptides, and recombinant proteins" ,, Q5 High-Fidelity MAster Mix,
New England BioLabs
,Cat#
M0492S
FreeStyle MAX,Thermo Fisher,Cat#16447500 Protein G agarose,
Pierce
,Cat#
20397
293F FreeStyle Expression
(37)
A genome-wide CRISPR screen maps endogenous regulators of PPARG gene expression in bladder cancer
iScience
May 19, 2023
Davide Tortora, Morgan E. Roberts, Gunjan Kumar, Sudha S. Kotapalli, Elie Ritch, Joshua M. Scurll, Brian McConeghy, Sunita Sinha, Alexander W. Wyatt, Peter C. Black,
et al.
assay kit,
ThermoFisher Scientific
,Cat#
Q33120
Q5 High-Fidelity 2X Master Mix
,
New England Biolabs
,Cat#
M0492L
Qiaquick PCR Purification Kit,
Qiagen
,Cat#
28104
AMPure XP Reagent,
Beckman Coulter
,Cat#
A63881
Bioanalyzer
(38)
Paths to adaptation under fluctuating nitrogen starvation: The spectrum of adaptive mutations in Saccharomyces cerevisiae is shaped by retrotransposons and microhomology-mediated recombination
PLOS Genetics
May 16, 2023
Michelle Hays, Katja Schwartz, Danica T. Schmidtke, Dimitra Aggeli, Gavin Sherlock
D2002). 75 ng of genomic DNA were used for
PCR amplification
in two 50 μL reactions with 25 μL 2xQ5 mix (
NEB
M0492
), 2.5μM of each forward (FOS) and reverse (ROS) primers (S2 Table) and water (to 50μl) for 22 cycles
(39)
Functionally distinct promoter classes initiate transcription via different mechanisms reflected in focused versus dispersed initiation patterns
The EMBO Journal
May 15, 2023
Leonid Serebreni, Lisa‐Marie Pleyer, Vanja Haberle, Oliver Hendy, Anna Vlasova, Vincent Loubiere, Filip Nemčko, Katharina Bergauer, Elisabeth Roitinger, Karl Mechtler,
et al.
Laemmli Sample Buffer
,
Bio‐Rad
,Cat#
1610737
EGTA
,
Merck
,Cat#E4378
Biotin‐11‐CTP
,
PerkinElmer
,Cat#
NEL542001EA
Biotin‐11‐UTP
,
PerkinElmer
,Cat#
NEL543001EA
Q5 polymerase high‐fidelity 2× master mix,
NEB
,Cat#
M0492S
Trizol,
Thermo Fisher
,Cat#
15596026
Trizol‐LS,
Thermo Fisher
,Cat#
10296010
GlycoBlue™ Coprecipitant,
Thermo Fisher
(40)
phox2ba: The Potential Genetic Link behind the Overlap in the Symptomatology between CHARGE and Central Congenital Hypoventilation Syndromes
Genes (Basel)
May 15, 2023
Jessica E. MacLean, Jaime N. Wertman, Sergey V. Prykhozhij, Emily Chedrawe, Stewart Langley, Shelby L. Steele, Kevin Ban, Kim Blake, Jason N. Berman
phox2ba_BamHI_linker_rev (GATCGGATCCACTGCCTCCACCGCCACACAGGCACACAGAATCAA) from cDNA using
Q5 High-Fidelity 2X Master Mix
(
M0492S
;
New England Biolabs
Inc., Ipswich, MA, USA) using the standard Q5 protocol with 65 °C annealing temperature.
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Reviews
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(1)
8/6/2019
PCR Master Mix for Superior Performance for a Broad Range of Amplicons (NEB)
Atul Singh
It works well for all kinds of PCR.
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Q5 High-Fidelity 2X Master Mix from New England Biolabs
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