Welcome Guest
Sign In
Register
Products
Popular Categories
Antibody Search
ELISA Kit Search
Biomolecules
Flow Cytometers
Thermal Cyclers
Microscopes
Transfection Reagents
Featured Products
New Products
Articles
Resources
eBooks
Bench Tips
Tech Insights
Editorial Articles
News
Product Reviews
Future Lab
Multimedia
Biocompare Webinars
Immunotherapy
Reproducibility
Transformative Technologies
Podcasts
Tech Talks
Bench Tip Videos
All Videos and Webinars
Life Science Webinars
Write a Review
Antibodies
Kits/Reagents
Equipment
Services
Resources
Promotions
Events
AACR SPOTLIGHT
Register
Sign In
Biocompare
Products
Cell Biology
Biomolecules
Biomolecules
Q5 High-Fidelity 2X Master Mix
Supplier Images
Q5 High-Fidelity 2X Master Mix
Q5 High-Fidelity 2X Master Mix from New England Biolabs
(1)
Write a review
Citations:
(304)
Supplier Page
Supplier Page from
New England Biolabs for
Q5 High-Fidelity 2X Master Mix
Get Pricing
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Q5 High-Fidelity 2X Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0492S
Quantity
100 reactions
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (304)
(81)
Retrotransposon instability dominates the acquired mutation landscape of mouse induced pluripotent stem cells
Nature communications
December 3, 2022
Gerdes P, Lim SM, Ewing AD, Larcombe MR, Chan D, Sanchez-Luque FJ, Walker L, Carleton AL, James C, Knaupp AS,
et al
.
DNA Technologies (IDT) and
PCR
products generated using Q5 DNA polymerase (
New England Biolabs
, Cat#:
M0492
). The mCherry coding sequence was synthesized with silent mutations ablating potential splice donor
(82)
Systematic decomposition of sequence determinants governing CRISPR/Cas9 specificity
Nature Communications
December 1, 2022
Rongjie Fu, Wei He, Jinzhuang Dou, Oscar D. Villarreal, Ella Bedford, Helen Wang, Connie Hou, Liang Zhang, Yalong Wang, Dacheng Ma,
et al.
dual-target I and D were synthesized (IDT) and amplified with
Q5 High-Fidelity DNA polymerase
(
NEB
#
M0492
). Meanwhile, gRNA I and D oligonucleotides (Sigma) were annealed and ligated to the
BsmBI
(
NEB
#
R0739
)-linearized
(83)
Enhancer RNAs stimulate Pol II pause release by harnessing multivalent interactions to NELF
Nature Communications
December 1, 2022
Vladyslava Gorbovytska et al.
Conc. (60 U;
M0437M
, NEB, Ipswich, MA). Libraries were then amplified with Q5 High-Fidelity Master Mix (
M0492S
,
NEB
, Ipswich, MA). Subsequently,
PCR
products were size-selected in 6% polyacrylamide TBE gel (210–350
(84)
Harnessing DSB repair to promote efficient homology-dependent and -independent prime editing
Nature Communications
December 1, 2022
Martin Peterka et al.
Q5 Hot Start High-Fidelity 2x Master Mix
(
M0494
,
NEB
). Amplicons for long-read
sequencing
were generated with Q5 High-Fidelity polymerase (
M0492S
,
NEB
) using primers #51-52 (Supplementary Data 1) and the following
PCR
protocol: 98 °C 30 s 30x (98
(85)
Ultra-rapid somatic variant detection via real-time targeted amplicon sequencing
Communications Biology
December 1, 2022
Jack Wadden, Brandon S. Newell, Joshua Bugbee, Vishal John, Amy K. Bruzek, Robert P. Dickson, Carl Koschmann, David Blaauw, Satish Narayanasamy, Reetuparna Das
Integrated DNA technologies.
PCR
was performed using New England Biolabs (NEB) Q5 2x Master mix (
NEB
#
M0492
) and standard primer concentrations. 30 ng of NA12878 DNA (Coriell Institute) was used as template and
(86)
A versatile active learning workflow for optimization of genetic and metabolic networks
Nature Communications
December 1, 2022
Amir Pandi et al.
transcription & translation unit (Fig. 4b), PCRs were performed using
Q5Ⓡ High-Fidelity 2X Master Mix
(
NEB
#
M0492L
), sfGfp as the template, and primers (Eurofins and Sigma-Aldrich) with overhangs harboring PT7, RBS,
(87)
NT-seq: a chemical-based sequencing method for genomic methylome profiling
Genome Biology
December 1, 2022
Xuwen Li, Shiyuan Guo, Yan Cui, Zijian Zhang, Xinlong Luo, Margarita T. Angelova, Laura F. Landweber, Yinsheng Wang, Tao P. Wu
E7645S
). Unmodified control DNA was made by amplifying adaptor-ligated DNA using Q5 DNA polymerase (
NEB
,
M0492S
). Both native and amplified DNA was first annealed to excessive protective adaptor sequences in 5 mM
(88)
Assembly of plant virus agroinfectious clones using biological material or DNA synthesis
STAR Protocols
December 1, 2022
Fabio Pasin
NEBuilder® HiFi DNA assembly master mix
(2×),
New England Biolabs
,
E2621S
Q5® high-fidelity 2× master mix,
New England Biolabs
,
M0492S
T4 DNA ligase
(400 U/μL),
New England Biolabs
,
M0202S
Wizard® Plus SV minipreps DNA purification system
,
Promega
,
A1330
2-(N-Morpholino)ethanesulfonic
(89)
Inducible and reversible RNA N6-methyladenosine editing
Nature Communications
December 1, 2022
Huaxia Shi, Ying Xu, Na Tian, Ming Yang, Fu-Sen Liang
were used to amplify PYL or ABI fragments with
Q5 High-Fidelity 2x Master Mix
(
New England BioLabs
, #
M0492S
), using
PCR
instrument (C1000 Touch Thermal Cycler). The
PCR
fragments were then inserted into linearized
(90)
Multiparametric and accurate functional analysis of genetic sequence variants using CRISPR-Select
Nature genetics
December 1, 2022
Niu Y, Ferreira Azevedo CA, Li X, Kamali E, Haagen Nielsen O, Storgaard Sørensen C, Frödin M.
after the 2A sequence (Addgene, 71783). Briefly, Q5 High-Fidelity 2× Master Mix (
New England Biolabs
,
M0492S
) was used to
PCR
amplify the coding sequence of Cas9 from the pSpCas9-2A-GFP (Addgene, 48138) and the
(91)
Daily dynamics of contrasting spring algal blooms in Santa Monica Bay (central Southern California Bight)
Environmental Microbiology
December 1, 2022
Gerid A. Ollison, Sarah K. Hu, Julie V. Hopper, Brittany P. Stewart, Jayme Smith, Jennifer L. Beatty, Laura K. Rink, David A. Caron
(5'‐ACTTTCGTTCTTGATYRA‐3′) primers (Stoeck et al., 2010) via
Q5 High‐Fidelity 2x Master Mix
(
NEB
, #
M0492S
).
PCR reactions
were carried out in two steps due to the difference in annealing temperature between
(92)
Dicer promotes genome stability via the bromodomain transcriptional co-activator BRD4
Nature Communications
December 1, 2022
M. J. Gutbrod, B. Roche, J. I. Steinberg, A. A. Lakhani, K. Chang, A. J. Schorn, R. A. Martienssen
necessary to maintain the representation of the library ~500x using the high fidelity Q5 polymerase (
NEB
M0492
) and Illumina-compatible barcoded primers were used in a second
PCR
to create libraries for each replicate
(93)
High resolution profiling of pathways of escape for SARS-CoV-2 spike-binding antibodies
bioRxiv
November 29, 2022
Meghan E. Garrett, Jared Galloway, Helen Y. Chu, Hannah L. Itell, Caitlin I. Stoddard, Caitlin R. Wolf, Jennifer K. Logue, Dylan McDonald, Frederick A. Matsen, Julie Overbaugh
Proteins","Chemicals, Peptides, and Recombinant Proteins"
Q5 High-Fidelity 2X Master Mix
,
NEB
,Cat. #
M0492S
Critical Commercial
Assays
,Critical Commercial
Assays
,Critical Commercial
Assays
QIAquick Gel Extraction
(94)
Ascertaining cells' synaptic connections and RNA expression simultaneously with barcoded rabies virus libraries
Nat Commun
November 16, 2022
Arpiar Saunders, Kee Wui Huang, Cassandra Vondrak, Christina Hughes, Karina Smolyar, Harsha Sen, Adrienne C. Philson, James Nemesh, Alec Wysoker, Seva Kashin,
et al.
Primer (10 μM; P7i’x‘-GFP or P7i’x‘-TCB_CreOn), 1;
Q5 High-Fidelity 2x Master Mix
(
New England Biolabs
,
M0492L
), 25; Water, 22. Cycle Conditions: (1) 98 °C for 30 s; (2) 98 °C for 10 s; (3) 72 °C for 30 s; (4) 72
(95)
Deconvolution of in vivo protein-RNA contacts using fractionated eCLIP-seq
STAR Protoc
November 16, 2022
Giulia Biancon, Emma Busarello, Poorval Joshi, Bluma J. Lesch, Stephanie Halene, Toma Tebaldi
iQ™ SYBR® Green Supermix
,
Bio-Rad
,"Cat#
1708880
; CAS: 56-81-5, 67-68-5" Q5® High-Fidelity 2× Master Mix,
New England Biolabs
,Cat#
M0492S
AMPure XP Reagent,
Beckman Coulter
,Cat#
A63880
"
Novex™ TBE-Urea Gels, 6%, 12 well
",
Thermo Fisher Scientific
,Cat#
EC68652BOX
"GelStar™
(96)
Functional characterization of diverse type I-F CRISPR-associated transposons
Nucleic Acids Research
November 11, 2022
Avery Roberts, Matthew A Nethery, Rodolphe Barrangou
multiple
cloning
site fragments were generated as
PCR
products using
Q5 High-Fidelity 2X Master Mix
(
NEB
#
M0492
) and assembled using
NEBuilder HiFi DNA Assembly Master Mix
(
NEB
#
E2621
). Further pEffector derivatives
(97)
Dual genome-wide coding and lncRNA screens in neural induction of induced pluripotent stem cells
Cell Genomics
November 9, 2022
David Wu, Aunoy Poddar, Elpiniki Ninou, Elizabeth Hwang, Mitchel A. Cole, S. John Liu, Max A. Horlbeck, Jin Chen, Joseph M. Replogle, Giovanni A. Carosso,
et al.
A1110501
TransIT-LT1,
Mirus Bio
,Cat#
MIR 2300
ViralBoost,Alstem Bio,Cat# VB100 Q5 High-Fidelity Master Mix,
NEB
,Cat#
M0492
,, Critical commercial
assays
,Critical commercial
assays
,Critical commercial
assays
,,
Direct-zol RNA Miniprep
(98)
Trans-regulatory changes underpin the evolution of the Drosophila immune response
PLoS Genet
November 7, 2022
Shuai Dominique Ding, Alexandre B. Leitão, Jonathan P. Day, Ramesh Arunkumar, Morgan Phillips, Shuyu Olivia Zhou, Francis M. Jiggins
amplified in 15 μL reactions containing 7.5 μL
Q5 High-Fidelity 2X Master Mix
(
New England Biolabs
M0492S
), 2 μL of template cDNA, 33 nM each primer and 4 μL of nuclease free water. Primers were designed to
(99)
Genome-wide base editor screen identifies regulators of protein abundance in yeast
Elife
November 3, 2022
Olga T Schubert, Joshua S Bloom, Meru J Sadhu, Leonid Kruglyak
amplified by a 15-cycle
PCR
with sublibrary-specific primers (Q5 High-Fidelity 2 X Master Mix by
NEB
#
M0492S
). After
DNA purification
(Zymo Research #
D4013
), a second 5-cycle
PCR
was performed with primers binding
(100)
The staphylococcal inhibitory protein SPIN binds to human myeloperoxidase with picomolar affinity but only dampens halide oxidation
Journal of Biological Chemistry
November 1, 2022
Urban Leitgeb, Paul G. Furtmüller, Stefan Hofbauer, Jose A. Brito, Christian Obinger, Vera Pfanzagl
(
NEB
#
E2621S
);
PCR amplification
of all fragments was done using Q5 High-Fidelity 2× Master Mix (
NEB
#
M0492L
) and primers 3′- TGAGGCTCACCGCGAACAGATTGGAGGTAAAGTTTATTCCCAGAAC-5′ and 3′- GTTCTGGGAATAAACTTTACCTCCAATCTGTTCGCGGTGAGCCTCA-5’
Page
<<
<
1
2
3
4
5
>
>>
Reviews
Write a Review
(1)
8/6/2019
PCR Master Mix for Superior Performance for a Broad Range of Amplicons (NEB)
Atul Singh
It works well for all kinds of PCR.
<<
<
>
>>
Q5 High-Fidelity 2X Master Mix from New England Biolabs
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
Back To Top