Welcome Guest
Sign In
Register
Products
Popular Categories
Antibody Search
ELISA Kit Search
Biomolecules
Flow Cytometers
Thermal Cyclers
Microscopes
Transfection Reagents
Featured Products
New Products
Articles
Resources
eBooks
Bench Tips
Tech Insights
Editorial Articles
News
Product Reviews
Future Lab
Multimedia
Biocompare Webinars
Immunotherapy
Reproducibility
Transformative Technologies
Podcasts
Tech Talks
Bench Tip Videos
All Videos and Webinars
Life Science Webinars
Write a Review
Antibodies
Kits/Reagents
Equipment
Services
Resources
Promotions
Events
AACR SPOTLIGHT
Register
Sign In
Biocompare
Products
PCR Master Mixes (High Fidelity)
Q5 High-Fidelity DNA Polymerase
Supplier Images
Q5 High-Fidelity DNA Polymerase
Q5 High-Fidelity DNA Polymerase from New England Biolabs
Be the first to write a review!
Citations:
(219)
Supplier Page
Supplier Page from
New England Biolabs for
Q5 High-Fidelity DNA Polymerase
Get Pricing
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Q5 High-Fidelity DNA Polymerase
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0491L
Quantity
500 units
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Type
DNA Polymerase
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (219)
(21)
Low-cost molecular methods to characterise gastrointestinal nematode co-infections of goats in Africa
Parasites & Vectors
June 29, 2023
Paul M. Airs, Javier Ventura-Cordero, Winchester Mvula, Taro Takahashi, Jan Van Wyk, Patson Nalivata, Andrews Safalaoh, Eric R. Morgan
nanograms of template DNA was added per 30 μl
PCR
reaction (
Q5® High-Fidelity DNA Polymerase
,
NEB
, cat.
M0491L
) with a mix of ITS-2 primers containing 0–3 ‘N’ bases and an Illumina adapter (10 μM stock) used at
(22)
Genome analysis of Cephalotrichum gorgonifer and identification of the biosynthetic pathway for rasfonin, an inhibitor of KRAS dependent cancer
Fungal Biology and Biotechnology
June 24, 2023
Andreas Schüller, Lena Studt-Reinhold, Harald Berger, Lucia Silvestrini, Roman Labuda, Ulrich Güldener, Markus Gorfer, Markus Bacher, Maria Doppler, Erika Gasparotto,
et al.
was either performed with the Q5®-polymerase from New England Biolabs (
New England Biolabs
; Art. No.
M0491L
) for
plasmid cloning
, or with the
GoTaq® Green Master Mix
(
Promega
; Madison, WI, USA; Art. No.
M7845
)
(23)
Novel Fas-TNFR chimeras that prevent Fas ligand-mediated kill and signal synergistically to enhance CAR T cell efficacy
Molecular Therapy - Nucleic Acids
June 13, 2023
Callum McKenzie, Mohamed El-Kholy, Farhaan Parekh, Mathew Robson, Katarina Lamb, Christopher Allen, James Sillibourne, Shaun Cordoba, Simon Thomas, Martin Pule
codon-optimized open reading frames (GeneArt), were synthesized (IDT) and amplified using Q5 DNA polymerase (
NEB
,
M0491L
) and oligonucleotide primers (IDT). The resulting
PCR
products were fractionated on an agarose gel,
(24)
Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelope
bioRxiv
June 13, 2023
Li-Chun Cheng, Xi Zhang, Sabyasachi Baboo, Julie A Nguyen, Salvador Martinez-Bartolomé, Esther Loose, Jolene Diedrich, John R Yates, Larry Gerace
Genes of interest were amplified by
PCR
using
Q5 High-Fidelity DNA Polymerase
(
New England Biolabs
#
M0491L
). All genes were inserted into either pLV-Ef1a-V5-LIC-IRES-PURO (Addgene #120247) or pLV-Ef1a-LIC-V5-IRES-PURO
(25)
A protein domain-oriented approach to expand the opportunities of therapeutic exon skipping for USH2A-associated retinitis pigmentosa
Molecular therapy. Nucleic acids
June 13, 2023
Schellens RTW, Broekman S, Peters T, Graave P, Malinar L, Venselaar H, Kremer H, De Vrieze E, Van Wijk E.
corresponding wild-type
zebrafish
ush2a alleles. For
PCR
analysis, the
Q5 High-Fidelity DNA Polymerase
kit (#
M0491L
;
New England Biolabs
, Ipswich, MA, USA) was employed. All primer sequences are listed in Table S2. The
(26)
Generation and Characterization of a Zebrafish Model for ADGRV1-Associated Retinal Dysfunction Using CRISPR/Cas9 Genome Editing Technology
Cells
June 10, 2023
Stemerdink M, Broekman S, Peters T, Kremer H, de Vrieze E, van Wijk E.
(
Applied Biosystems
, Waltham, MA, USA) using
Q5® High-Fidelity DNA Polymerase
(
New England Biolabs
, Ipswich, MA, USA, #
M0491L
) and 1× EvaGreen Dye (Biotium, Fremont, CA, USA, #31000). Primers that were used for the
PCR amplification
(27)
Biochemical Correction of GM2 Ganglioside Accumulation in AB-Variant GM2 Gangliosidosis
International Journal of Molecular Sciences
May 24, 2023
Natalie M. Deschenes, Camilyn Cheng, Alex E. Ryckman, Brianna M. Quinville, Prem Khanal, Melissa Mitchell, Zhilin Chen, Waheed Sangrar, Steven J. Gray, Jagdeep S. Walia
punch samples obtained at 21 days of age. Briefly, DNA was digested using Q5® High-Fidelity Master Mix (
M0491L
;
New England BioLabs
Ltd., Whitby, ON, Canada) and subsequently analysed by
PCR
using the following
(28)
Structure of Anabaena flos-aquae gas vesicles revealed by cryo-ET
Structure
May 4, 2023
Przemysław Dutka, Lauren Ann Ann Metskas, Robert C. Hurt, Hossein Salahshoor, Ting-Yu Wang, Dina Malounda, George J. Lu, Tsui-Fen Chou, Mikhail G. Shapiro, Grant J. Jensen
proteins","Chemicals, peptides, and recombinant proteins" ,,
Q5 High-Fidelity DNA Polymerase
,
New England Biolabs
,Cat#
M0491L
NEBuilder HiFi DNA Assembly Master Mix
,
New England Biolabs
,Cat#
E2621L
LB Broth (Lennox
),
Sigma-Aldrich
,Cat#
(29)
Membranous NECTIN-4 Expression Frequently Decreases during Metastatic Spread of Urothelial Carcinoma and Is Associated with Enfortumab Vedotin Resistance
Clinical Cancer Research
April 14, 2023
Niklas Klümper, Damian J. Ralser, Jörg Ellinger, Florian Roghmann, Julia Albrecht, Eduard Below, Abdullah Alajati, Danijel Sikic, Johannes Breyer, Christian Bolenz,
et al.
amplified with 22 cycles using approximately 20 to 50 ng of gDNA in a 15-μL mixture with Q5 polymerase (
M0491L
,
New England Biolabs
) according to the manufacturer's protocol. Subsequently, 2 μL was transferred to
(30)
Multicellular, IVT-derived, unmodified human transcriptome for nanopore direct RNA analysis
bioRxiv
April 13, 2023
Caroline A. McCormick, Stuart Akeson, Sepideh Tavakoli, Dylan Bloch, Isabel N. Klink, Miten Jain, Sara H. Rouhanifard
These primers are listed in Supplementary Data 8. The
PCR
reaction was set up with Q5 polymerase (
NEB
,
M0491L
) using the Manufacturer’s protocol. Thermocycling conditions were as follows: initial denaturation at
(31)
CCR4‐NOT subunit CCF‐1/CNOT7 promotes transcriptional activation to multiple stress responses in Caenorhabditis elegans
Aging Cell
April 1, 2023
Hadi Tabarraei, Brandon M. Waddell, Kelly Raymond, Sydney M. Murray, Ying Wang, Keith P. Choe, Cheng‐Wei Wu
full‐length ccf‐1 cDNA
clone
was generated through
PCR
using the
Q5® High‐Fidelity DNA polymerase
(
M0491L
;
New England BioLabs
) and cloned into the pDEST32 vector containing the GAL4 DNA binding domain (DBD)
(32)
Genetic diversity in the metronidazole metabolism genes nitroreductases and pyruvate ferredoxin oxidoreductases in susceptible and refractory clinical samples of Giardia lamblia
International Journal for Parasitology: Drugs and Drug Resistance
April 1, 2023
Christina S. Saghaug, Astrid L. Gamlem, Kirsti B. Hauge, Juha Vahokoski, Christian Klotz, Toni Aebischer, Nina Langeland, Kurt Hanevik
and NR2 genes. All gene-PCRs were carried out using the
Q5® High-Fidelity DNA Polymerase
(catalog nr
M0491L
,
New England BioLabs
(NEB) Ipswich, MA, USA) and reaction setup for 25 μl according to manufacturer.
(33)
Initial ciliary assembly in Chlamydomonas requires Arp2/3 complex–dependent endocytosis
Molecular Biology of the Cell
April 1, 2023
Brae M. Bigge, Nicholas E. Rosenthal, Prachee Avasthi
ARPC4 genetic sequence was isolated from
Chlamydomonas
cDNA using
PCR
with the Q5 DNA Polymerase (
NEB
;
M0491L
). The resulting fragment and the pChlamy4 plasmid (
Thermofisher
; A24231) were digested with
EcoRI
(
NEB
;
(34)
A-MYB and BRDT-dependent RNA Polymerase II pause release orchestrates transcriptional regulation in mammalian meiosis
Nature Communications
March 29, 2023
Adriana K. Alexander, Edward J. Rice, Jelena Lujic, Leah E. Simon, Stephanie Tanis, Gilad Barshad, Lina Zhu, Jyoti Lama, Paula E. Cohen, Charles G. Danko
transcription reaction in order to generate cDNA (
ThermoFisher
#
18090010
). The cDNA was amplified (
NEB
M0491L
) in order to generate the leChRO-seq libraries which were prepared using manufacturer’s protocol (Illumina)
(35)
Characterization of the oral and gut microbiome in children with obesity aged 3 to 5 years
Frontiers in Cellular and Infection Microbiology
March 29, 2023
Ting Ma, Zeyu Wu, Jing Lin, Chao Shan, Aisaiti Abasijiang, Jin Zhao
Amplification
of bacterial 16S rRNA was performed using
PCR
with an
NEB
Q5 High-Fidelity polymerase (
M0491L
). Selected primers were F: AGAGTTTGATCMTGGCTCAG; R: ACCTTGTTACGACTT. The target fragment obtained via
(36)
Developmental mRNA clearance by PIWI-bound endo-siRNAs in Paramecium
Cell Reports
March 28, 2023
Therese Solberg, Victor Mason, Chundi Wang, Mariusz Nowacki
assays
,Critical commercial
assays
,Critical commercial
assays
,,
Q5 high fidelity DNA polymerase
,
NEB
,Cat#
M0491L
GoTaq G2 DNA polymerase
,
Promega
,Cat#
M7848
RadPrime DNA Labeling System
,
Invitrogen
,Cat#
18428011
QIAGEN
(37)
CRISPR-induced DNA reorganization for multiplexed nucleic acid detection
Nature Communications
March 17, 2023
Margot Karlikow, Evan Amalfitano, Xiaolong Yang, Jennifer Doucet, Abigail Chapman, Peivand Sadat Mousavi, Paige Homme, Polina Sutyrina, Winston Chan, Sofia Lemak,
et al.
during RePAIR. Synthetic DNA for DWV, IAPV and rpl8 sequences were ordered and amplified by
PCR
(
NEB
Q5
M0491L
) using their corresponding primers (Supplementary Data 1). For
in vitro transcription
, synthetic DNA
(38)
Diverse branching forms regulated by a core auxin transport mechanism in plants
Development
March 15, 2023
Victoria M. R. Spencer, Lucy Bentall, C. Jill Harrison
(https://eu.idtdna.com/pages/tools/oligoanalyzer?returnurl=%2Fcalc%2Fanalyzer).
PCR
was performed with S. kraussiana cDNA and Q5® HF DNA Polymerase (
New England Biolabs
;
M0491L
).
PCR
products were separated by
gel electrophoresis
and extracted, incubated with
Taq Polymerase
for
(39)
Characterization of the humanized FRG mouse model and development of an AAV-LK03 variant with improved liver lobular biodistribution
Molecular therapy. Methods & clinical development
March 9, 2023
Cabanes-Creus M, Navarro RG, Liao SHY, Scott S, Carlessi R, Roca-Pinilla R, Knight M, Baltazar G, Zhu E, Jones M,
et al
.
150-bp region surrounding the 6-mer barcode was amplified with
Q5 High-Fidelity DNA Polymerase
(
NEB
; #
M0491L
) using BC_F (5′-GCT GGA GTT CGT GAC CGC CG) and BC_R primer (5′-CAA CAT AGT TAA GAA TAC CAG TCA ATC
(40)
Cardiomyocyte Apoptosis Is Associated with Contractile Dysfunction in Stem Cell Model of MYH7 E848G Hypertrophic Cardiomyopathy
International journal of molecular sciences
March 3, 2023
Loiben AM, Chien WM, Friedman CE, Chao LS, Weber G, Goldstein A, Sniadecki NJ, Murry CE, Yang KC.
containing mutation was amplified through
PCR
using
Q5 High-Fidelity DNA Polymerase
(
New England Biolabs
,
M0491L
, Ipswich, MA, USA) and 500 nM forward and reverse primers (Table 3).
PCR
products were run on 1% agarose
Page
<<
<
1
2
3
4
5
>
>>
Reviews
Be the First to Write a Review!
Q5 High-Fidelity DNA Polymerase from New England Biolabs
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
Back To Top