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PCR Master Mixes (High Fidelity)
Q5 High-Fidelity DNA Polymerase
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Q5 High-Fidelity DNA Polymerase
Q5 High-Fidelity DNA Polymerase from New England Biolabs
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Citations:
(214)
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New England Biolabs for
Q5 High-Fidelity DNA Polymerase
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Company Info
Citations
Reviews
Product Specs
Item
Q5 High-Fidelity DNA Polymerase
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0491L
Quantity
500 units
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Type
DNA Polymerase
Temperature
-20 C
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (214)
(1)
An isothermal CRISPR-based diagnostic assay for Neisseria gonorrhoeae and Chlamydia trachomatis detection
Microbiology Spectrum
December 12, 2023
Hao Luo, Lihong Zeng, Xiaona Yin, Yuying Pan, Jianjiang Yang, Mingjing Liu, Xiaolin Qin, Zhanqin Feng, Wentao Chen, Heping Zheng
or Cas13a. dsDNA was prepared using Q5 High-Fidelity DNA Polymerases (
New England Biolabs
, MA, USA;
M0491L
) to amplify the target in a 50-µL solution (10 µL of 5× Q5 Reaction Buffer, 10 µL of 5× Q5 High GC Enhancer,
(2)
Epigenetic modifications regulate cultivar-specific root development and metabolic adaptation to nitrogen availability in wheat
Nat Commun
December 12, 2023
Hao Zhang, Zhiyuan Jin, Fa Cui, Long Zhao, Xiaoyu Zhang, Jinchao Chen, Jing Zhang, Yanyan Li, Yongpeng Li, Yanxiao Niu,
et al.
and resuspended in ddH2O. The library was amplified 17 cycles by Q5 high-fidelity polymerase (
NEB
,
M0491L
). Antibodies (H3K27me3, CST, C36B11, 1/50 dilution; H3K4me3, Abcam, ab8582, 1/50 dilution; H3K27ac,
(3)
In vivo RNA interactome profiling reveals 3'UTR-processed small RNA targeting a central regulatory hub
Nat Commun
December 7, 2023
Fang Liu, Ziying Chen, Shuo Zhang, Kejing Wu, Cheng Bei, Chuan Wang, Yanjie Chao
Libraries were
PCR
amplified with Illumina P5 and P7 primers using
Q5 High-Fidelity DNA Polymerase
(#
M0491L
,
NEB
), and purified with 1.5x RNAclean XP beads. The libraries were sequenced by 150 bp paired-end
sequencing
(4)
High-throughput functional analysis of regulatory variants using a massively parallel reporter assay
STAR Protocols
December 1, 2023
Kate Delfosse, Chiara Gerhardinger, John L. Rinn, Philipp G. Maass
proteins","Chemicals, peptides, and recombinant proteins" ,,
Q5 high-fidelity DNA polymerase
,
NEB
,Cat#
M0491L
Acetylated BSA,
Invitrogen
,Cat#
AM2614
2-Butanol,Sigma-Aldrich,Cat#294810-1L PhiX control v3,Illumina,Cat#FC-110-3001
SfiI
,
NEB
,Cat#
R0123L
"Alkaline
(5)
Biased placement of Mitochondria fission facilitates asymmetric inheritance of protein aggregates during yeast cell division
PLOS Computational Biology
November 27, 2023
Gordon Sun, Christine Hwang, Tony Jung, Jian Liu, Rong Li
by Gibson Assembly (
NEB
E5510S
).
Q5 high-fidelity DNA polymerase
was used for all
PCR reactions
(
NEB
M0491L
). For N-terminal tagging of
yeast
proteins with GFP, the gene of interest was cloned with its native
(6)
Jorvik: A membrane-containing phage that will likely found a new family within Vinavirales
iScience
November 17, 2023
Pavol Bárdy, Conor I.W. MacDonald, Roman Pantůček, Alfred A. Antson, Paul C.M. Fogg
Limited,Cat#132950-1L RNase A,VWR International,Cat#A2760.0100 Q5 DNA polymerase,
New England Biolabs
,Cat#
M0491L
SafeBLUE stain,NBS Biologicals,Cat#NBS-SB1L Dnase I,, PEG8000,
Promega
,Cat#
V3011
EcoRV
,
New England Biolabs
(7)
MiRNA-Responsive CRISPR-Cas System via a DNA Regulator
Biosensors (Basel)
November 7, 2023
Dayoung Yun, Cheulhee Jung
region was amplified from 150 ng of gDNA using
Q5 High Fidelity DNA polymerase
(
NEB
, Hitchin, UK, cat#
M0491L
) and primers. The
PCR
program was as follows: 98 °C for 30 s; 30 cycles of 98 °C for 10 s, 65 °C for
(8)
Insights from the Construction of Adenovirus-Based Vaccine Candidates against SARS-CoV-2: Expecting the Unexpected
Viruses
October 25, 2023
Denice Weklak, Julian Tisborn, Maurin Helen Mangold, Raphael Scheu, Harald Wodrich, Claudia Hagedorn, Franziska Jönsson, Florian Kreppel
recombination were generated using Q5® high-fidelity polymerase (
New England Biolabs
GmbH, Ipswich, MA, USA,
M0491L
) with primers including 50 bp homology arms to the insertion site. Thus, 10 ng DNA template were mixed
(9)
ASCT2 is the primary serine transporter in cancer cells
bioRxiv
October 17, 2023
Kelly O. Conger, Christopher Chidley, Mete Emir Ozgurses, Huiping Zhao, Yumi Kim, Svetlana E. Semina, Philippa Burns, Vipin Rawat, Ryan Sheldon, Issam Ben-Sahra,
et al.
amplified using 23 cycles of
PCR
on the gDNA from at least 6 million cells as template, Q5 polymerase (
NEB
M0491L
) and barcoded forward and reverse primers. Amplified
PCR
products (~240–250 bp) were purified by
agarose gel electrophoresis
(10)
Salicylic acid and RNA interference mediate antiviral immunity of plant stem cells
Proceedings of the National Academy of Sciences
October 17, 2023
Marco Incarbone, Gabriele Bradamante, Florian Pruckner, Tobias Wegscheider, Wilfried Rozhon, Vu Nguyen, Ruben Gutzat, Zsuzsanna Mérai, Thomas Lendl, Stuart MacFarlane,
et al.
vectors were made by amplifying the sequences of interest by
PCR
using Q5 HiFi DNA polymerase (
NEB
#
M0491L
) with primers containing the
BsaI
cut site in the appropriate orientation and the standard “sticky ends”
(11)
Highly pathogenic avian influenza A (H5N1) in marine mammals and seabirds in Peru
Nature Communications
September 7, 2023
Mariana Leguia, Alejandra Garcia-Glaessner, Breno Muñoz-Saavedra, Diana Juarez, Patricia Barrera, Carlos Calvo-Mac, Javier Jara, Walter Silva, Karl Ploog, Lady Amaro,
et al.
using Superscript IV (
Invitrogen
18090050
) and amplified using
Q5 High-fidelity DNA polymerase
(
NEB
M0491L
).
Amplification
products were prepared into barcoded
sequencing
libraries using DNA Prep Kits (Illumina
(12)
The viral fitness and intrinsic pathogenicity of dominant SARS-CoV-2 Omicron sublineages BA.1, BA.2, and BA.5
EBioMedicine
September 1, 2023
Shuai H, Chan JF, Hu B, Chai Y, Yoon C, Liu H, Liu Y, Shi J, Zhu T, Hu JC,
et al
.
Transcriptor First Strand cDNA Synthesis Kit
(04897030001,
Roche
, USA). The viral genome was amplified using
Q5® High-Fidelity DNA Polymerase
(
M0491L
,
New England Biolabs
, USA) with ARTIC network nCoV-2019 primers v4 (Integrated DNA Technologies, USA).
(13)
Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelope
Life Science Alliance
September 1, 2023
Li-Chun Cheng, Xi Zhang, Sabyasachi Baboo, Julie A Nguyen, Salvador Martinez-Bartolomé, Esther Loose, Jolene Diedrich, John R Yates, Larry Gerace
from the C3H cells. Genes of interest were amplified by
PCR
using
Q5 high-fidelity DNA polymerase
(#
M0491L
;
New England Biolabs
). All genes were inserted into either pLV-Ef1a-V5-LIC-IRES-PURO (#120247; Addgene)
(14)
Evolution of molecular switches for regulation of transgene expression by clinically licensed gluconate
Nucleic Acids Research
August 25, 2023
Ana Palma Teixeira, Shuai Xue, Jinbo Huang, Martin Fussenegger
T4 DNA ligase
(
EL0011
,
Thermo Fisher
). All
PCR reactions
were performed using
Q5 High-Fidelity DNA polymerase
(
M0491L
,
New England Biolabs
). After
ligation
, the plasmids were transformed and amplified in
E. coli
XL10-Gold
(15)
Protocol for bulk and single-nuclei chromatin accessibility quantification in mouse liver tissue
STAR Protoc
August 16, 2023
Noga Korenfeld, Nicolaj I. Toft, Trine V. Dam, Meital Charni-Natan, Lars Grøntved, Ido Goldstein
Nuclease-free water
,Hylabs,BP731/500D Digitonin,
Sigma
,D141-100MG
Tween-20
,Avantor,X251-07 MinElute PCR purification kit,
Qiagen
,20-
28006
Q5 High-Fidelity DNA Polymerase
,
New England Biolabs
,
M0491L
Q5 Reaction Buffer,
New England Biolabs
,
B9027S
PCR
dNTP’s Mix,Larova,VAR-02 Agencourt AMPure XP beads,
Beckman Coulter
(16)
Cold temperature induces a TRPM8-independent calcium release from the endoplasmic reticulum in human platelets
bioRxiv
July 22, 2023
Anastasiia Stratiievska, Olga Filippova, Tahsin Özpolat, Daire Byrne, S. Lawrence Lawrence Bailey, Molly Y. Mollica, Jeff Harris, Kali Esancy, Junmei Chen, Ajay K. Dhaka,
et al.
5’-CAGGCTGAGCGATGAAATGCTGATCTG-3’
PCR amplification
was carried out using a
Q5® High-Fidelity DNA Polymerase
kit (#
M0491L
,
New England Biolabs
, Ipswich, MA) using the following protocol: 40 cycles of 30-sec denaturation at
(17)
Polycomb repressive complex 2 binds and stabilizes NANOG to suppress differentiation-related genes to promote self-renewal
iScience
July 21, 2023
Yeh DW, Liu C, Hernandez JC, Tahara SM, Tsukamoto H, Machida K.
Stellar™ Competent Cells
,
Takara
,
636766
Advantage® GC 2
PCR Kit
,Takara,639119
Q5® High-Fidelity DNA Polymerase
,
New England BioLabs
,
M0491L
T4 DNA Ligase
,
New England BioLabs
,
M0202L
NEBuilder® HiFi DNA Assembly Master Mix
,
New England BioLabs
,
E2621L
Quick Blunting™ Kit
(18)
Systematic characterization of regulatory variants of blood pressure genes
Cell Genomics
July 12, 2023
Winona Oliveros, Kate Delfosse, Daniella F. Lato, Katerina Kiriakopulos, Milad Mokhtaridoost, Abdelrahman Said, Brandon J. McMurray, Jared W.L. Browning, Kaia Mattioli, Guoliang Meng,
et al.
T4 DNA Ligase
,
NEB
,Cat#
M0202M
XbaI
,
NEB
,Cat#
R0145L
KpnI-HF
,
NEB
,Cat#
R3142L
Q5- High Fidelity DNA polymerase
,
NEB
,Cat#
M0491L
Formamide (Deionized
),
Invitrogen
,Cat#
AM9342
Agencourt AMPure XP,
Beckman Coulter
,Cat#
A63881
Trizol Reagent
(19)
Low-cost molecular methods to characterise gastrointestinal nematode co-infections of goats in Africa
Parasites & Vectors
June 29, 2023
Paul M. Airs, Javier Ventura-Cordero, Winchester Mvula, Taro Takahashi, Jan Van Wyk, Patson Nalivata, Andrews Safalaoh, Eric R. Morgan
nanograms of template DNA was added per 30 μl
PCR
reaction (
Q5® High-Fidelity DNA Polymerase
,
NEB
, cat.
M0491L
) with a mix of ITS-2 primers containing 0–3 ‘N’ bases and an Illumina adapter (10 μM stock) used at
(20)
Genome analysis of Cephalotrichum gorgonifer and identification of the biosynthetic pathway for rasfonin, an inhibitor of KRAS dependent cancer
Fungal Biology and Biotechnology
June 24, 2023
Andreas Schüller, Lena Studt-Reinhold, Harald Berger, Lucia Silvestrini, Roman Labuda, Ulrich Güldener, Markus Gorfer, Markus Bacher, Maria Doppler, Erika Gasparotto,
et al.
was either performed with the Q5®-polymerase from New England Biolabs (
New England Biolabs
; Art. No.
M0491L
) for
plasmid cloning
, or with the
GoTaq® Green Master Mix
(
Promega
; Madison, WI, USA; Art. No.
M7845
)
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Q5 High-Fidelity DNA Polymerase from New England Biolabs
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Company Info
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