EpiMarkHot StartTaqDNA Polymerase M0490S from New England Biolabs

Product Specs
ItemEpiMarkHot StartTaqDNA Polymerase
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0490S
Quantity100 reactions
ApplicationsDNA Analysis
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeDNA Polymerase
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Investigating pioneer factor activity and its coordination with chromatin remodelers using integrated synthetic oligo assaySTAR ProtocolsJune 7, 2023Hengye Chen, Chao Yan, Archana Dhasarathy, Michael Kladde, Lu BaiB0202S β-Mercaptoethanol,Sigma,Cat# M3148 Turbo Cx Pfu,Agilent,Cat# 600410 EpiMark® Hot Start Taq DNA Polymerase,NEB,Cat# M0490S 5 M Ammonium acetate,Thermo Scientific,Cat# J60688-AE RNase A,VWR,Cat#100218-566 Phenol:chloroform:isoamyl

(2) NT-seq: a chemical-based sequencing method for genomic methylome profilingGenome BiologyDecember 1, 2022Xuwen Li, Shiyuan Guo, Yan Cui, Zijian Zhang, Xinlong Luo, Margarita T. Angelova, Laura F. Landweber, Yinsheng Wang, Tao P. WuD4060). Illumina TruSeq adaptor was added to oligo by PCR amplification using Taq DNA polymerase (NEB, M0490S) (cycle number was determined by qPCR with iTaq polymerase (BioRad, 1725121)). The indexed library was

(3) Partitioned usage of chromatin remodelers by nucleosome-displacing factorsCell ReportsAugust 23, 2022Hengye Chen, Hungyo Kharerin, Archana Dhasarathy, Michael Kladde, Lu BaiIgG Sepharose™ 6 Fast Flow,Cytiva,Cat#17-0969-01 Turbo Cx Pfu,Agilent,Cat#600410 EpiMark® Hot Start Taq DNA Polymerase,NEB,Cat#M0490S Rapamycin,Life Technologies,Cat#PHZ1235 17β-estradiol,Tocris Bioscience,Cat#50-28-2 Critical commercial

(4) Cannabinoid CB2 receptors are upregulated via bivalent histone modifications and control primary afferent input to the spinal cord in neuropathic painJournal of Biological ChemistryJune 1, 2022Krishna Ghosh, Guang-Fen Zhang, Hong Chen, Shao-Rui Chen, Hui-Lin Panbp to +134 bp region of the CB2 promoter were amplified using EpiMark Hot Start Taq DNA Polymerase (#M0490S, New England Biolabs). The following primers were used for the qPCR: Cnr2 (-300/-108 bp) forward, 5′-AGA

(5) Terminal modification, sequence, length, and PIWI-protein identity determine piRNA stabilityMolecular cellDecember 2, 2021Gainetdinov I, Colpan C, Cecchini K, Arif A, Jouravleva K, Albosta P, Vega-Badillo J, Lee Y, Özata DM, Zamore PD.two nested rounds (H19 and IAP LTR elements) of PCR with EpiMark Hot Start Taq DNA Polymerase (NEB, M0490S) using the following protocol: initial denaturation, 95°C for 30 sec; 35 cycles of 95°C for 30 sec,

(6) Epigenomic Regulators Elongator Complex Subunit 2 and Methyltransferase 1 Differentially Condition the Spaceflight Response in ArabidopsisFrontiers in Plant ScienceSeptember 13, 2021Anna-Lisa Paul, Natasha Haveman, Brandon Califar, Robert J. Ferlcycle amplification using a uracil-insensitive polymerase (EpiMark hot start Taq polymerase, NEB, Cat #M0490S). The amplified library products were separated on a 2% agarose gel from which library fragments in

(7) Decitabine treatment demethylates vast majority of high-confidence differentially methylated regions in HCT-116 colorectal cancer cellsF1000ResearchAugust 4, 2020Anna Sandler et al.step 2 39 times Table 3. PCR reagents. Reagent vol (ul) 5x epimark Taq buffer (New England Biolabs; M0490S) 4 diH2O 13.5 10 mM dNTPs (New England Biolabs; N0447S) 0.4 genomic DNA (8 ng / ul) 1 Primer

(8) Cell-specific exon methylation and CTCF binding in neurons regulate calcium ion channel splicing and functioneLifeMarch 26, 2020Eduardo Javier López Soto, Diane LipscombedNTPs; 1.25 units EpiMark Hot Start Taq DNA Polymerase and 1X reaction buffer (New England Biolabs Cat# M0490S); and H2O in a C1000 Touch Thermal Cycler (BIO RAD). PCR reactions consisted of 1 min of initial incubation

(9) Single-Cell Transcriptomic Analyses of Cell Fate Transitions during Human Cardiac ReprogrammingCell stem cellJuly 3, 2019Zhou Y, Liu Z, Welch JD, Gao X, Wang L, Garbutt T, Keepers B, Ma H, Prins JF, Shen W, et al.Kits,Qiagen,69504 EZ DNA Methylation-Gold Kit,Zymo Research,D5005 EpiMark Hot Start Taq DNA Polymerase,NEB,M0490S NearIR live/dead dye,Thermo Fisher Scientific,L10119 Carboxyfluorescein succinimidyl ester (CFSE)

(10) Aptamers in the Therapeutics and Diagnostics PipelinesTheranosticsJuly 1, 2018Harleen Kaur, John G. Bruno, Amit Kumar, Tarun Kumar Sharmaother molecular biology reagents, has commercialized Hot Start Taq DNA polymerase (Cat No. M0496S and M0490S). Both of these products principally rely on an aptamer-based hot start technology to avoid nonspecific

(11) Hypomethylation of intron1 of α-synuclein gene does not correlate with Parkinson’s diseaseMolecular BrainDecember 1, 2017Subhrangshu Guhathakurta, Baggio A. Evangelista, Susmita Ghosh, Sambuddha Basu, Yoon-Seong Kimprimers as described by Jowaed et al. [1]. EpiMark Hot Start Taq DNA polymerase (NEB Inc; Catalogue No. M0490S) was used for the PCR amplification. The amplified PCR products of 444 base pair (Fig.1a) were then

(12) A novel tool for monitoring endogenous alpha-synuclein transcription by NanoLuciferase tag insertion at the 3′end using CRISPR-Cas9 genome editing techniqueScientific ReportsApril 4, 2017Sambuddha Basu, Levi Adams, Subhrangshu Guhathakurta, Yoon-Seong KimDNA as described in article by Jowaed et al.8. EpiMark Hot Start Taq DNA Polymerase (NEB Inc; cat No. M0490S) was used for PCR and the amplicon was cloned into pGEM -T Easy vector (Promega Corporation). 10 positive

(13) Methylated DNA is over-represented in whole-genome bisulfite sequencing dataFrontiers in GeneticsOctober 21, 2014Lexiang Ji, Takahiko Sasaki, Xiaoxiao Sun, Ping Ma, Zachary A. Lewis, Robert J. Schmitz(Agilent Technologies, Santa Clara, CA, cat-600410, USA), and EpiMark (New England Biolabs., Ipswich, MA, cat-M0490S, USA). PCR cycle number varied from 4, 8, or 15 cycles as described in the main text. PREPARATION AND

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EpiMarkHot StartTaqDNA Polymerase

EpiMarkHot StartTaqDNA Polymerase from New England Biolabs

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EpiMarkHot StartTaqDNA Polymerase from New England Biolabs

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