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NEBNext High-Fidelity 2X PCR Master Mix
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NEBNext High-Fidelity 2X PCR Master Mix
NEBNext High-Fidelity 2X PCR Master Mix from New England Biolabs
Be the first to write a review!
Citations:
(491)
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Supplier Page from
New England Biolabs for
NEBNext High-Fidelity 2X PCR Master Mix
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
NEBNext High-Fidelity 2X PCR Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0541S
Quantity
50 reactions
Applications
DNA Analysis, PCR
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (491)
(101)
Optimized single-nucleus transcriptional profiling by combinatorial indexing
Nature Protocols
January 1, 2023
Beth K. Martin, Chengxiang Qiu, Eva Nichols, Melissa Phung, Rula Green-Gladden, Sanjay Srivatsan, Ronnie Blecher-Gonen, Brian J. Beliveau, Cole Trapnell, Junyue Cao,
et al.
(
New England Biolabs
, cat. no.
E6111L
)
NEBNext high fidelity 2x PCR master mix
(
New England Biolabs
, cat. no.
M0541L
) dNTP mix (
New England Biolabs
, cat. no.
N0447L
) Agencourt AMPure XP (
Beckman Coulter
, cat. no.
A63882
) YoYo
(102)
A chromatin accessibility landscape during early adipogenesis of human adipose-derived stem cells
Adipocyte
December 31, 2022
Sen Li, Xiaolin Zong, Liheng Zhang, Luya Li, Jianxin Wu
(#28,004, Qiagen). Then, samples were amplified by using 1X NEBNext High-Fidelity PCR Master Mix (#
M0541L
,
New England Biolabs
), the subsequent library was purified by the MinElute PCR Purification Kit (#28,004,
(103)
In vivo, genome-wide profiling of endogenously tagged chromatin-binding proteins with spatial and temporal resolution using NanoDam in Drosophila
STAR Protoc
December 16, 2022
Jocelyn L.Y. Tang, Robert Krautz, Oriol Llorà -Batlle, Anna E. Hakes, Paul M. Fox, Andrea H. Brand
HS DNA Polymerase,
Bioline
,Cat#
BIO-21113
NEBNext High Fidelity 2× PCR Master Mix (6.25 mL),
NEB
,Cat#
M0541L
PEG-8000,Sigma-Aldrich,Cat#P5413 Phosphate Buffer Saline (1× PBS),N/A,N/A RNAseA (DNAse free),
Roche
,Cat#
11119915001
T4 DNA Ligase
(104)
Mouse kidney nuclear isolation and library preparation for single-cell combinatorial indexing RNA sequencing
STAR Protocols
December 16, 2022
Haikuo Li, Benjamin D. Humphreys
enzyme,
NEB
,
E6111L
Dimethylformamide,
Thermo Scientific
,Cat#
20673
USER enzyme,
NEB
,
M5505L
NEBNext High-Fidelity 2× PCR Master Mix,
NEB
,
M0541L
DNA binding buffer,Zymo,D4004-1-L Nuclease-free H2O,
Invitrogen
,
AM9932
Unloaded Tn5 transposase,Diagenode,C01070002
Glycerol
,
Sigma
,G5516-1L Elution
(105)
Dynamic chromatin accessibility deploys heterotypic cis/trans-acting factors driving stomatal cell-fate commitment
Nature Plants
December 15, 2022
Eun-Deok Kim, Michael W. Dorrity, Bridget A. Fitzgerald, Hyemin Seo, Krishna Mohan Sepuru, Christine Queitsch, Nobutaka Mitsuda, Soon-Ki Han, Keiko U. Torii
MinElute PCR purification kit (
Qiagen
,
28006
) and then amplified using
High Fidelity PCR
Mix (
NEB
,
M0541
, 0061704) with custom bar-coded primer for 8–11 total
PCR
cycles. Amplified
ATAC-seq
libraries were
(106)
PAX5 epigenetically orchestrates CD58 transcription and modulates blinatumomab response in acute lymphoblastic leukemia
Science Advances
December 14, 2022
Yizhen Li, Takaya Moriyama, Satoshi Yoshimura, Xujie Zhao, Zhenhua Li, Xu Yang, Elisabeth Paietta, Mark R. Litzow, Marina Konopleva, Jiyang Yu,
et al.
Nucleic Acid Gel Stain (Invitrogen, S-7563), and 25 μl of NEBNext High-Fidelity 2× PCR Master Mix (
NEB
,
M0541
).
PCR
cycle was as follows: 72°C for 5 min; 98°C for 30 s; five repeats of 98°C for 10 s, 63°C for 30
(107)
Genome-wide gain-of-function screening characterized lncRNA regulators for tumor immune response
Science advances
December 9, 2022
Wang Y, Zhao Y, Guo W, Yadav GS, Bhaskarla C, Wang Z, Wang X, Li S, Wang Y, Chen Y,
et al
.
Then, the extracted genomic DNA was used for
PCR
using NEBNext High Fidelity 2× PCR Master Mix (
NEB
,
M0541S
). The
Next-Generation sequencing (NGS
) primers that amplify the sgRNA library were listed in the protocol.
(108)
Comprehensive single-cell transcriptional profiling defines shared and unique epithelial injury responses during kidney fibrosis
Cell metabolism
December 6, 2022
Li H, Dixon EE, Wu H, Humphreys BD.
reaction, the plate was first incubated at 80°C for 10 minutes. Then, 20 μl NEBnext 2× master mix (
M0541L
,
NEB
), 2 μl 10 μM P5 primer and 2 μl 10 μM P7 primer were added into each well. Primer sequences can
(109)
BAF Complex Maintains Glioma Stem Cells in Pediatric H3K27M Glioma
Cancer discovery
December 2, 2022
Panditharatna E, Marques JG, Wang T, Trissal MC, Liu I, Jiang L, Beck A, Groves A, Dharia NV, Li D,
et al
.
(cat. #
28004
,
Qiagen
) and
PCR
preamplified in 5 cycles using NEBNext-Fidelity 2× PCR Master Mix (cat. #
M0541S
,
NEB
) and primers containing Illumina adapter sequences before the number of additional cycles was assessed
(110)
Tracking cryptic SARS-CoV-2 lineages detected in NYC wastewater
Nature Communications
December 1, 2022
Davida S. Smyth, Monica Trujillo, Devon A. Gregory, Kristen Cheung, Anna Gao, Maddie Graham, Yue Guan, Caitlyn Guldenpfennig, Irene Hoxie, Sherin Kannoly,
et al.
each), dNTPs (100 µM each) (
New England Biolabs
,
N0447L
) and Q5 DNA polymerase (
New England Biolabs
,
M0541S
). Secondary
PCR amplification
was performed as follows: 95 °C (2:00) + [95 °C (0:15) + 55 °C (0:30)
(111)
PARP1-SNAI2 transcription axis drives resistance to PARP inhibitor, Talazoparib
Scientific Reports
December 1, 2022
Xia Ding et al.
purification kit (
28004
,
Qiagen
). The libraries were amplified using
NEBNext High-Fidelity 2X PCR Master Mix
(
M0541
,
NEB
) with primer extension at 72 °C for 5 min, denaturation at 98 °C for 30 s, followed by 8 cycles
(112)
Stress induced aging in mouse eye
Aging cell
December 1, 2022
Xu Q, Rydz C, Nguyen Huu VA, Rocha L, Palomino La Torre C, Lee I, Cho W, Jabari M, Donello J, Lyon DC,
et al
.
with Nextera compatible indexed
sequencing
i5 and i7 adapters using NEBNext 2x PCR Master Mix
PCR kit
(
M0541
,
NEB
). The amplified DNA library was fragment size selected from 200 to 800 bp using Ampure XP beads
(113)
SHAPE-guided RNA structure homology search and motif discovery
Nature Communications
December 1, 2022
Edoardo Morandi, Martijn J. van Hemert, Danny Incarnato
purification
, barcodes were introduced by
PCR
, using
NEBNext® High-Fidelity 2X PCR Master Mix
(
NEB
, cat.
M0541L
) and NEBNext®
Multiplex
Oligos for Illumina® Dual-Index primers (
NEB
, cat.
E7780S
). Optimization of
(114)
Multidimensional chromatin profiling of zebrafish pancreas to uncover and investigate disease-relevant enhancers
Nature Communications
December 1, 2022
Renata Bordeira-Carriço et al.
(#FC-121-1030, Illumina). Libraries were amplified using
NEBNext® High-Fidelity 2X PCR Master Mix
(#
M0541S
,
NEB
) with primers Ad1, Ad2.23 and Ad2.2445. The final product was purified with DNA Clean and Concentrator
(115)
ARID1A loss induces polymorphonuclear myeloid-derived suppressor cell chemotaxis and promotes prostate cancer progression
Nature communications
November 26, 2022
Li N, Liu Q, Han Y, Pei S, Cheng B, Xu J, Miao X, Pan Q, Wang H, Guo J,
et al
.
FC-121-1031). Samples were purified and
PCR
-amplified with the
NEBNext High-Fidelity 2X PCR Master Mix
(
NEB
;
M0541L
).
ATAC-seq
libraries were sequenced on the Illumina HiSeq 2500 (125-nucleotide read length, paired end).
(116)
Dynamic quality control machinery that operates across compartmental borders mediates the degradation of mammalian nuclear membrane proteins
Cell Reports
November 22, 2022
Pei-Ling Tsai, Christopher J.F. Cameron, Maria Fernanda Forni, Renee R. Wasko, Brigitte S. Naughton, Valerie Horsley, Mark B. Gerstein, Christian Schlieker
Fugene HD Transfection Reagent
,
Promega
,Cat#
E2311
NEB Next High Fidelity PCR Master Mix (2X),
New England BioLabs
,Cat#
M0541L
Flouromount-G,
Southern Biotech
,Cat#
0100–01
Complete protease
inhibitor
cocktail,Roche,Cat# 11836170001
Nonidet P40 Substitute
(117)
Mapping open chromatin by ATAC-seq in bread wheat
Frontiers in Plant Science
November 16, 2022
Xin Wang, Chuanye Chen, Chao He, Dijun Chen, Wenhao Yan
either TruePrep
DNA Library Prep
Kit V2 (Vazyme, #TD501) or NEBNext High-Fidelity2X PCR Master Mix (
NEB
,
M0541
). The primer sequences are listed in Table S1 . The precise number of
PCR
cycles was determined by
(118)
ADRA1A–Gαq signalling potentiates adipocyte thermogenesis through CKB and TNAP
Nature Metabolism
November 7, 2022
Janane F. Rahbani, Charlotte Scholtes, Damien M. Lagarde, Mohammed F. Hussain, Anna Roesler, Christien B. Dykstra, Jakub Bunk, Bozena Samborska, Shannon L. O’Brien, Emma Tripp,
et al.
DNA was eluted in 21 μl elution buffer and amplified for five cycles using NEBNext 2× MasterMix (
NEB
,
M0541L
) as previously described58 using published primers59. Then, 10% of the pre-amplified mixture was used
(119)
On-microscope staging of live cells reveals changes in the dynamics of transcriptional bursting during differentiation
Nat Commun
November 4, 2022
D. M. Jeziorska, E. A. J. Tunnacliffe, J. M. Brown, H. Ayyub, J. Sloane-Stanley, J. A. Sharpe, B. C. Lagerholm, C. Babbs, A. J. H. Smith, V. J. Buckle,
et al.
columns (
28206
,
Qiagen
).
PCR
indexing was performed using NEBNext High-Fidelity 2× PCR Master Mix (
M0541S
,
NEB
) and sequenced using a NextSeq platform (Illumina). After
sequencing
, read quality was assessed
(120)
The rearing environment persistently modulates mouse phenotypes from the molecular to the behavioural level
PLOS Biology
October 21, 2022
Ivana Jaric, Bernhard Voelkl, Melanie Clerc, Marc W. Schmid, Janja Novak, Marianna Rosso, Reto Rufener, Vanessa Tabea von Kortzfleisch, S. Helene Richter, Manuela Buettner,
et al.
each sample: 10 μl transposed DNA, 25 μl NEBNext High-Fidelity 2× PCR Master Mix (
New England Biolabs
,
M0541S
), 9 μl of unique, dual-indexed primer (IDT for Illumina Nextera DNA UD Indexes; 20026930), and 6 μl
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NEBNext High-Fidelity 2X PCR Master Mix from New England Biolabs
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View Company Product Page
Pricing Info
Product Specs
Company Info
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