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NEBNext High-Fidelity 2X PCR Master Mix
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NEBNext High-Fidelity 2X PCR Master Mix
NEBNext High-Fidelity 2X PCR Master Mix from New England Biolabs
Be the first to write a review!
Citations:
(491)
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Supplier Page from
New England Biolabs for
NEBNext High-Fidelity 2X PCR Master Mix
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Company Info
Citations
Reviews
Product Specs
Item
NEBNext High-Fidelity 2X PCR Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0541S
Quantity
50 reactions
Applications
DNA Analysis, PCR
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (491)
(81)
Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation
Nature cell biology
March 1, 2023
Wong YF, Kumar Y, Proks M, Herrera JAR, Rothová MM, Monteiro RS, Pozzi S, Jennings RE, Hanley NA, Bickmore WA,
et al
.
reaction was assembled containing 10 µl of tagmented DNA, 25 µl NEBNext
High-Fidelity PCR
Mix (
NEB
,
M0541S
), 5 µl of SYBR Green (
Invitrogen
,
S7563
) and index primers at 2 µM concentration. Ten microlitres of
(82)
Age-dependent Lamin changes induce cardiac dysfunction via dysregulation of cardiac transcriptional programs
Nature Aging
February 27, 2023
Natalie J. Kirkland, Scott H. Skalak, Alexander J. Whitehead, James D. Hocker, Pranjali Beri, Geo Vogler, Bill Hum, Mingyi Wang, Edward G. Lakatta, Bing Ren,
et al.
(
28004
,
QIAGEN
). The resulting libraries were amplified using
NEBNext High-Fidelity 2X PCR Master Mix
(
M0541
,
NEB
) with primer extension at 72°C for 5 minutes, denaturation at 98°C for 30 s, followed by 8 cycles
(83)
eRNA profiling uncovers the enhancer landscape of oesophageal adenocarcinoma and reveals new deregulated pathways
eLife
February 20, 2023
Ibrahim Ahmed, Shen-Hsi Yang, Samuel Ogden, Wei Zhang, Yaoyong Li, Andrew D The OCCAMs consortium, Andrew D The OCCAMs consortium
Index kit,Illumina,FC-121-1012, Commercial
assay
or kit,
NEBNext high fidelity 2x PCR master mix
,
NEB
,
M0541
, Commercial
assay
or kit,DNA Clean and Concentrator,Zymo,D4013, Commercial
assay
or kit,Polyfect,
Qiagen
,
301107
, Commercial
(84)
A single‐cell RNA labeling strategy for measuring stress response upon tissue dissociation
Molecular Systems Biology
February 10, 2023
Anika Neuschulz, Olga Bakina, Victor Badillo‐Lisakowski, Pedro Olivares‐Chauvet, Thomas Conrad, Michael Gotthardt, Helmut Kettenmann, Jan Philipp Junker
we added library PCR mix (10 μl nuclease free water, 25 μl NEBNext PCR mix 2× (
New England Biolabs
#
M0541L
), 2.5 μl RP1 primer (10 μM), 2.5 μl RPI index primer (10 μM)) and amplified using the following program:
(85)
Differential modularity of the mammalian Engrailed 1 enhancer network directs sweat gland development
PLOS Genetics
February 6, 2023
Daniel Aldea, Blerina Kokalari, Yuji Atsuta, Heather L. Dingwall, Ying Zheng, Arben Nace, George Cotsarelis, Yana G. Kamberov
PowerUP SYBR Green master mix
,
ThermoFisher Scientific
,REF #
A25742
NEBNext High-Fidelity 2X PCR Master Mix
,
New England Biolabs
,REF #
M0541S
DIG RNA Labeling Kit (SP6/T7
),
Roche
,REF #
11175025910
Experimental models
: Cell lines,
Experimental models
(86)
High-content image-based CRISPR screening reveals regulators of 3D genome architectures
bioRxiv
February 4, 2023
Mengwei Hu, Bing Yang, Tyler B Jensen, Jonathan S D Radda, Yubao Cheng, Shengyan Jin, Siyuan Wang
forward and reverse primers, 5% DMSO and 1× NEBNext High-Fidelity PCR master mix (
New England BioLabs
,
M0541L
) following the manufacturer’s instructions. The
PCR
primers contained flow-cell binding sequence,
sequencing
(87)
Neonatal imprinting of alveolar macrophages via neutrophil-derived 12-HETE
Nature
February 1, 2023
Pernet E, Sun S, Sarden N, Gona S, Nguyen A, Khan N, Mawhinney M, Tran KA, Chronopoulos J, Amberkar D,
et al
.
kit and amplified with Nextera
PCR
primers (Illumina Nextera Index kit) and NEBNext PCR master mix (
M0541
,
New England BioLabs
). Amplified DNA was purified with a Qiagen Mini Elute kit. Libraries were sequenced
(88)
RNA Polymerase II, the BAF remodeler and transcription factors synergize to evict nucleosomes
bioRxiv : the preprint server for biology
January 22, 2023
Brahma S, Henikoff S.
water
bath
for 15 min. Libraries were amplified using NEBNext HiFi 2X PCR Master mix (
NEB
cat. no.
M0541
) with 13 rounds of
amplification
as described previously28.
Sequencing
libraries were purified using
(89)
Expansion of interferon inducible gene pool via USP18 inhibition promotes cancer cell pyroptosis
Nature communications
January 17, 2023
Arimoto KI, Miyauchi S, Troutman TD, Zhang Y, Liu M, Stoner SA, Davis AG, Fan JB, Huang YJ, Yan M,
et al
.
purification kit (
28004
,
Qiagen
). The libraries were amplified using NEBNext High-Fidelity 2× PCR Master Mix (
M0541
,
NEB
) with primer extension at 72 °C for 5 min and denaturation at 98 °C for 30 s, followed by 8 cycles
(90)
A genome-wide relay of signalling-responsive enhancers drives hematopoietic specification
Nature communications
January 17, 2023
Edginton-White B, Maytum A, Kellaway SG, Goode DK, Keane P, Pagnuco I, Assi SA, Ames L, Clarke M, Cockerill PN,
et al
.
µl, 25 µM AttB tn5 Fwd primer 5 µl, 25 µM AttB tn5 Rev primer 5 µl, NEBNext Master Mix 50 µl (
NEB
,
M0541
)) with the following conditions, 72 °C for 5 min, 98 °C for 30 s, 5 cycles of 98 °C 10 s, 63 °C 30 s
(91)
Comparison of a Genotype 1 and a Genotype 2 Macaque Foamy Virus env Gene Indicates Distinct Infectivity and Cell-Cell Fusion but Similar Tropism and Restriction of Cell Entry by Interferon-Induced Transmembrane Proteins
Viruses
January 17, 2023
Fricke T, Schlagowski S, Liu S, Yang X, Fiebig U, Kaul A, Ensser A, Hahn AS.
primers (Integrated DNA Technologies, Coralville, IA, USA) using the NEBNext® HiFi 2 × PCR Master Mix (
M0541
,
NEB
) according to the manufacturer’s protocols. The libraries were cleaned up with AMPure XP beads
(92)
Multiomic identification of key transcriptional regulatory programs during endurance exercise training
bioRxiv
January 15, 2023
Gregory R. Smith, Bingqing Zhao, Malene E. Lindholm, Archana Raja, Mark Viggars, Hanna Pincas, Nicole R. Gay, Yifei Sun, Yongchao Ge, Venugopalan D. Nair,
et al.
Purification
kits (
Qiagen
#
28006
), and amplified using
NEBnext High-Fidelity 2x PCR Master Mix
(
NEB
,
M0541L
) and custom indexed primers65. 1.8x SPRIselect beads were used to clean the
PCR
reaction and remove
(93)
DNA dioxygenases Tet2/3 regulate gene promoter accessibility and chromatin topology in lineage-specific loci to control epithelial differentiation
Science advances
January 13, 2023
Chen GD, Fatima I, Xu Q, Rozhkova E, Fessing MY, Mardaryev AN, Sharov AA, Xu GL, Botchkarev VA.
purification
, the DNA was amplified with NEBNext High-Fidelity 2× PCR Master Mix [New England Biolabs (
NEB
), #
M0541
], as well as with unique i5 and i7 index primers for 10 cycles to prepare DNA library. Paired-end
sequencing
(94)
TGFB1 Induces Fetal Reprogramming and Enhances Intestinal Regeneration
bioRxiv : the preprint server for biology
January 13, 2023
Chen L, Dupre A, Qiu X, Pellon-Cardenas O, Walton KD, Wang J, Perekatt AO, Hu W, Spence JR, Verzi MP.
(
QIAGEN
REF
28004
), and
PCR
was amplified with high-fidelity 2x PCR Master Mix (
New England Biolabs
M0541
). One-third of the maximum fluorescent intensity during a
qPCR
trial run was used to determine the additional
(95)
ChIATAC is an efficient strategy for multi-omics mapping of 3D epigenomes from low-cell inputs
Nature Communications
January 13, 2023
Haoxi Chai, Harianto Tjong, Peng Li, Wei Liao, Ping Wang, Chee Hong Wong, Chew Yee Ngan, Warren J. Leonard, Chia-Lin Wei, Yijun Ruan
resuspended in Buffer EB. DNA was amplified by adding 25 μl NEBNext® High-Fidelity 2× PCR Master Mix (
NEB
;
M0541S
), 5 μl Illumina Nextara XT i7 index primer, and 5 μl Illumina Nextara XT i5 index primer with the following
(96)
Reconstructing mutational lineages in breast cancer by multi-patient-targeted single-cell DNA sequencing
Cell Genomics
January 11, 2023
Jake Leighton, Min Hu, Emi Sei, Funda Meric-Bernstam, Nicholas E. Navin
E6050L
Quick
Ligation
,
NEB
,Cat#
E6056L
dA-tailing,
NEB
,Cat#
E6053L
NEBNext HiFi2x PCRmix,
NEB
,Cat#
M0541L
Nimblegen SeqCap
EZ Exome V2 kit,
Roche
,Cat# 05860482001 Mission Bio Custom Panel,Mission Bio,Cat#
(97)
Whole-genome functional characterization of RE1 silencers using a modified massively parallel reporter assay
Cell Genomics
January 11, 2023
Kousuke Mouri, Hannah B. Dewey, Rodrigo Castro, Daniel Berenzy, Susan Kales, Ryan Tewhey
cycles of 12
PCR reactions
each 50 μL in volume containing oligos, 25 μL of Q5 NEBNext Master Mix (
NEB
,
M0541
) and 0.5 μM forward and reverse primers (Integrated DNA Technologies, IDT) (primers 1 and 3 for the
(98)
Developmental cell fate choice in neural tube progenitors employs two distinct cis-regulatory strategies
Developmental Cell
January 9, 2023
M. Joaquina Joaquina Delás, Christos M. Kalaitzis, Tamara Fawzi, Madeleine Demuth, Isabel Zhang, Hannah T. Stuart, Elena Costantini, Kenzo Ivanovitch, Elly M. Tanaka, James Briscoe
processed. Library generation Transposed DNA was first amplified with 2X PCR Master Mix
NEB
(Cat. No.
M0541S
), and 1 μl of constant forward primer and indexed reversed primer (Table S1) (both at 25 μM) in a total
(99)
ZBTB18 restricts chromatin accessibility and prevents transcriptional adaptations that drive metastasis
Science advances
January 6, 2023
Wang R, Bhatt AB, Minden-Birkenmaier BA, Travis OK, Tiwari S, Jia H, Rosikiewicz W, Martinot O, Childs E, Loesch R,
et al
.
(
Zymo Research
, #
D4013
), the libraries were amplified by
PCR
using the NEBNext HiFi 2× PCR Master Mix (
NEB
, #
M0541S
).
PCR
fragments were purified with the QIAGEN MinElute PCR Purification Kit, and the library concentration
(100)
The aryl hydrocarbon receptor cell intrinsically promotes resident memory CD8+ T cell differentiation and function
Cell Reports
January 4, 2023
Joseph W. Dean, Eric Y. Helm, Zheng Fu, Lifeng Xiong, Na Sun, Kristen N. Oliff, Marcus Muehlbauer, Dorina Avram, Liang Zhou
DNA Library Preparation
Kit,Illumina,Cat# FC-121-1030
NEBNext High-Fidelity 2X PCR Master Mix
,
NEB
,Cat#
M0541
SMART-Seq HT Kit,Takara,Cat# 634456 iDeal
ChIP-Seq
,
Diagenode
,Cat#
C01010055
GoScript Reverse Transcriptase
,
Promega
,Cat#
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NEBNext High-Fidelity 2X PCR Master Mix from New England Biolabs
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