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NEBNext High-Fidelity 2X PCR Master Mix
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NEBNext High-Fidelity 2X PCR Master Mix
NEBNext High-Fidelity 2X PCR Master Mix from New England Biolabs
Be the first to write a review!
Citations:
(491)
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New England Biolabs for
NEBNext High-Fidelity 2X PCR Master Mix
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
NEBNext High-Fidelity 2X PCR Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0541S
Quantity
50 reactions
Applications
DNA Analysis, PCR
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (491)
(61)
Identification of indocyanine green as a STT3B inhibitor against mushroom α-amanitin cytotoxicity
Nature communications
May 16, 2023
Wang B, Wan AH, Xu Y, Zhang RX, Zhao BC, Zhao XY, Shi YC, Zhang X, Xue Y, Luo Y,
et al
.
manufacturer’s protocol. The sgRNA sequences were amplified using High-Fidelity 2X PCR Master Mix (#
M0541L
,
NEB
).
PCR
products were gel extracted, quantified, mixed and sequenced using Illumina (PE150) by Novogene
(62)
SGRN: A Cas12a-driven Synthetic Gene Regulatory Network System
bioRxiv
May 11, 2023
HyunJin Kang, John C. Fitch, Reeba P. Varghese, Curtis A. Thorne, Darren A. Cusanovich
ordered from Operon. General
PCR
for
cloning
was performed using the NEBNext PCR master mix (
NEB
cat no.
M0541L
), with 500 nM of forward and reverse primers, 10 ng of template plasmid with the following cycling conditions
(63)
Combinatorial quantification of 5mC and 5hmC at individual CpG dyads and the transcriptome in single cells reveals modulators of DNA methylation maintenance fidelity
bioRxiv
May 11, 2023
Alex Chialastri, Saumya Sarkar, Elizabeth E. Schauer, Shyl Lamba, Siddharth S. Dey
was then used in a linear
PCR
reaction by adding 15 µL of PCR mix (1.67x
high-fidelity PCR
mix (
NEB
,
M0541L
) and 0.67 µM Extended RPI primer: 5’- AATGATACGGCGACCACCGAGATCTACACGTTCAGAGTTCTACAGTCCGACGATCGGTGTA
(64)
Genome-wide CRISPR/Cas9 screen identifies etoposide response modulators associated with clinical outcomes in pediatric AML
Blood Advances
May 9, 2023
Nam H. K. Nguyen, Roya Rafiee, Abderrahmane Tagmount, Amin Sobh, Alex Loguinov, Angelica K. de Jesus Sosa, Abdelrahman H. Elsayed, Mohammed O Gbadamosi, Nathan D Seligson, Christopher R. Cogle,
et al.
reaction with 12 reactions per sample) was amplified using
NEBNext High-Fidelity 2X PCR Master Mix
(#
M0541L
,
New England Biolabs
) kit with
PCR
conditions as follows: 98°C/3 min; 25 cycles of 98°C/10 s, 60°C/17
(65)
Optimized metrics for orthogonal combinatorial CRISPR screens
Scientific Reports
May 6, 2023
Ronay Cetin, Martin Wegner, Leah Luwisch, Sarada Saud, Tatjana Achmedov, Sebastian Süsser, Antonella Vera-Guapi, Konstantin Müller, Yves Matthess, Eva Quandt,
et al.
the plasmid DNA was mixed with 25 µl of
NEBNext High-Fidelity 2X PCR Master Mix
(
New England Biolabs
,
M0541
) and 2.5 µl each primer (10 µm), then water was added to a final volume of 50 µl.
PCR
was performed
(66)
TAL1 activation in T-cell acute lymphoblastic leukemia: a novel oncogenic 3’ neo-enhancer
Haematologica
May 1, 2023
Charlotte Smith, Ashish Goyal, Dieter Weichenhan, Eric Allemand, Anand Mayakonda, Umut Toprak, Anna Riedel, Estelle Balducci, Manisha Manojkumar, Anastasija Pejkovska,
et al.
50 mL reaction mixes consisting of 20 mL tagmented DNA eluate, 25 mL NEBNext High Fidelity 2X Mix (#
M0541
,
New England Biolabs
; Ipswich, MA, USA), 0.5 mL 100x SYBRGreen, 2.5 mL primer Tn5McP1n and 2.5 mL barcode
(67)
Precise modulation of transcription factor levels identifies features underlying dosage sensitivity
Nature genetics
May 1, 2023
Naqvi S, Kim S, Hoskens H, Matthews HS, Spritz RA, Klein OD, Hallgrímsson B, Swigut T, Claes P, Pritchard JK,
et al
.
Zymo DNA Clean & Concentrator kit and
PCR
-amplified with NEBNext High-Fidelity 2× PCR Master Mix (
NEB
,
M0541L
) and primers as defined in ref. 62. Libraries were purified by two rounds of double-sided size selection
(68)
An optimized approach for multiplexing single-nuclear ATAC-seq using oligonucleotide-conjugated antibodies
Epigenetics & Chromatin
April 28, 2023
Betelehem Solomon Bera, Taylor V. Thompson, Eric Sosa, Hiroko Nomaru, David Reynolds, Robert A. Dubin, Shahina B. Maqbool, Deyou Zheng, Bernice E. Morrow, John M. Greally,
et al.
sample: 10 µL of transposed DNA, 25 μl of NEBNext High-Fidelity 2 × PCR Master Mix (
New England Biolabs
,
M0541S
), 2.5 µL each of Nextera i5 and i7 indexed
amplification
primers (Nextera Index Kit, Illumina, FC-121-1011)
(69)
CHD-associated enhancers shape human cardiomyocyte lineage commitment
eLife
April 25, 2023
Daniel A Armendariz, Sean C Goetsch, Anjana Sundarrajan, Sushama Sivakumar, Yihan Wang, Shiqi Xie, Nikhil V Munshi, Gary C Hon
Kit,
Thermo Fisher
,Cat#88-18005-210, "Chemical compound, drug",NEBNext High-Fidelity,
New England Biolabs
,Cat#
M0541L
, "Chemical compound, drug",
Gibson Assembly Master Mix
,
New England Biolabs
,Cat#
E2611L
, "Chemical compound,
(70)
OBERON3 and SUPPRESSOR OF MAX2 1-LIKE proteins form a regulatory module driving phloem development
Nature Communications
April 14, 2023
Eva-Sophie Wallner, Nina Tonn, Dongbo Shi, Laura Luzzietti, Friederike Wanke, Pascal Hunziker, Yingqiang Xu, Ilona Jung, Vadir Lopéz-Salmerón, Michael Gebert,
et al.
2.5 µl 25 µM Custom Nextera
PCR
Primer 2 and 25 µl NEB Next High Fidelity 2x PCR Master Mix (
NEB
, #
M0541
) with 1 cycle of (72 °C for 5 min, 98 °C for 30 s) and 16 cycles of (98 °C for 10 s, 63 °C for 30 s,
(71)
Programmable protein delivery with a bacterial contractile injection system
Nature
April 13, 2023
Joseph Kreitz, Mirco J. Friedrich, Akash Guru, Blake Lash, Makoto Saito, Rhiannon K. Macrae, Feng Zhang
extract (see ‘
In vitro
PVC delivery experiments’) with NEBNext High-Fidelity 2× PCR Master Mix (
NEB
M0541
). Target regions were then barcoded with indexed Illumina P5 and P7
NGS
primers. Libraries were purified
(72)
FIPRESCI: droplet microfluidics based combinatorial indexing for massive-scale 5′-end single-cell RNA sequencing
Genome Biology
April 6, 2023
Yun Li, Zheng Huang, Zhaojun Zhang, Qifei Wang, Fengxian Li, Shufang Wang, Xin Ji, Shaokun Shu, Xiangdong Fang, Lan Jiang
eluted in 15 µl nuclease-free water. Then added 25 µl NEBNext High-Fidelity 2 × PCR Master Mix (
NEB
,
M0541S
), 5 µl 10 µM S-P7 (CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T), and 5 µl nuclease-free
(73)
Chemical modulation of Schistosoma mansoni lysine specific demethylase 1 (SmLSD1) induces wide-scale biological and epigenomic changes
Wellcome Open Research
March 30, 2023
Gilda Padalino, Cassandra A. Celatka, Hugh Y. Rienhoff Jr., Jay H. Kalin, Philip A. Cole, Damien Lassalle, Josephine Forde-Thomas, Iain W. Chalmers, Andrea Brancale, Christoph Grunau,
et al.
A preliminary 5-cycle
amplification
was carried out using
NEBNext High-Fidelity 2X PCR Master Mix
(
M0541S
,
New England Biolabs
) in a final volume of 50 μl. Following that, a
qPCR
side reaction was prepared
(74)
CRISPR screens identify gene targets at breast cancer risk loci
Genome Biology
March 29, 2023
Natasha K. Tuano, Jonathan Beesley, Murray Manning, Wei Shi, Laura Perlaza-Jimenez, Luis F. Malaver-Ortega, Jacob M. Paynter, Debra Black, Andrew Civitarese, Karen McCue,
et al.
enrichment [38].
PCR
1: 5
ng of 10 × cDNA was amplified using NEBNext high fidelity 2 × PCR mix (
NEB
#
M0541
) and the following primers: Rxn1_Fwd: TTTCCCATGATTCCTTCATATTTGC, Rxn1_Rev: ACACTCTTTCCCTACACGACG. Cycling
(75)
Guide-specific loss of efficiency and off-target reduction with Cas9 variants
bioRxiv
March 21, 2023
Liang Zhang, Wei He, Rongjie Fu, Han Xu
pairs listed in Supplementary Table S7 with 16 cycles using NEBNext High Fidelity Master Mix (
NEB
, #
M0541
) for the screens with EpiC and Tiling libraries and
NEBNext Ultra II Q5 Master Mix
(
NEB
, #M0544) for
(76)
hPSC-derived sacral neural crest enables rescue in a severe model of Hirschsprung's disease
Cell stem cell
March 2, 2023
Fan Y, Hackland J, Baggiolini A, Hung LY, Zhao H, Zumbo P, Oberst P, Minotti AP, Hergenreder E, Najjar S,
et al
.
amplified for 5 cycles using
NEBNext High-Fidelity 2X PCR Master Mix
(
New England Biolabs
catalog#
M0541L
). After evaluation by
real-time PCR
, 7-10 additional
PCR
cycles were done. The final product was cleaned
(77)
Efficient bi-allelic tagging in human induced pluripotent stem cells using CRISPR
STAR Protocols
March 1, 2023
Xingjie Ren, Maya Asami Takagi, Yin Shen
Wizard SV Genomic DNA Purification System
,
Promega
,Cat#
A2360
NEBNext High-Fidelity 2×
PCR Master Mix
,
NEB
,Cat#
M0541S
NEBuilder HiFi DNA Assembly Master Mix
,
NEB
,Cat#
E2621S
GoTaq Green Master Mix
,
Promega
,Cat#
M7123
Precision
(78)
Functional E3 ligase hotspots and resistance mechanisms to small-molecule degraders
Nature Chemical Biology
March 1, 2023
Alexander Hanzl, Ryan Casement, Hana Imrichova, Scott J. Hughes, Eleonora Barone, Andrea Testa, Sophie Bauer, Jane Wright, Matthias Brand, Alessio Ciulli,
et al.
blocking oligos. Post-capture
PCR
was performed with the
NEBNext High-Fidelity 2X PCR Master Mix
(
NEB
,
M0541S
) for 14-20 cycles.
Sequencing
libraries were quantified using the Qubit dsDNA HS Kit (Thermo Fisher
(79)
A Monoclonal Human Alveolar Epithelial Cell Line (“Arlo”) with Pronounced Barrier Function for Studying Drug Permeability and Viral Infections
Advanced Science
March 1, 2023
Patrick Carius, Annemarie Jungmann, Marco Bechtel, Alexander Grißmer, Annette Boese, Gilles Gasparoni, Abdulrahman Salhab, Ralf Seipelt, Klaus Urbschat, Clémentine Richter,
et al.
performed in a 30 µL reaction using the
NEBNext High‐Fidelity 2X PCR Master Mix
(
New England Biolabs
,
M0541S
) and 0.33 × 10−6 m indexed adapters (5′AATGATACGGCGACCACCGAGATCTACAC[i5]TCGTCGGCAGCGTC and 5″CAAGCAGAAGACGGCATACGAGAT[i7]GTCTCGTGGGCTCGG;
(80)
Tn5 tagments and transposes oligos to single-stranded DNA for strand-specific RNA sequencing
Genome Research
March 1, 2023
Yanjun Zhang, Yin Tang, Zhongxing Sun, Junqi Jia, Yuan Fang, Xinyi Wan, Dong Fang
in 19 μL H2O and mixed with 5 μL
PCR
primer mix, 25 μL NEBNext high-fidelity 2× PCR master mix (
NEB
M0541S
), 1 μL heat-labile UDG. Samples were amplified for 10 min at 37°C; for 30 sec at 98°C; 10 cycles of
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NEBNext High-Fidelity 2X PCR Master Mix from New England Biolabs
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