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NEBNext High-Fidelity 2X PCR Master Mix
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NEBNext High-Fidelity 2X PCR Master Mix
NEBNext High-Fidelity 2X PCR Master Mix from New England Biolabs
Be the first to write a review!
Citations:
(491)
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New England Biolabs for
NEBNext High-Fidelity 2X PCR Master Mix
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Company Info
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Reviews
Product Specs
Item
NEBNext High-Fidelity 2X PCR Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0541S
Quantity
50 reactions
Applications
DNA Analysis, PCR
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (491)
(41)
Genome-wide CRISPR screens define determinants of epithelial-mesenchymal transition mediated immune evasion by pancreatic cancer cells
Science Advances
July 14, 2023
Yuanzhuo Gu, Zhengkui Zhang, Marcel G. M. Camps, Ferry Ossendorp, Ruud H. Wijdeven, Peter ten Dijke
(
51194
,
Qiagen
). sgRNA sequences were recovered by
PCR
using
NEBNext High-Fidelity 2x PCR Master Mix
(
M0541L
,
New England BioLabs
) with primers as described in https://addgene.org/browse/article/16517/.
Sequencing
(42)
Relaxed targeting rules help PIWI proteins silence transposons
Nature
July 13, 2023
Ildar Gainetdinov, Joel Vega-Badillo, Katharine Cecchini, Ayca Bagci, Cansu Colpan, Dipayan De, Shannon Bailey, Amena Arif, Pei-Hsuan Wu, Ian J. MacRae,
et al.
Ampure XP beads (
Beckman Coulter
,
A63880
), cDNA was
PCR
amplified using NEBNext High-Fidelity (
NEB
,
M0541
; 98 °C for 30 s; 4 cycles of 98 °C for 10 s, 59 °C for 30 s, 72 °C for 12 s; 6 cycles of 98 °C for 10
(43)
Integrative Multi-Omics Analysis of Oncogenic EZH2 Mutants: From Epigenetic Reprogramming to Molecular Signatures
International Journal of Molecular Sciences
July 12, 2023
Julian Aldana, Miranda L. Gardner, Michael A. Freitas
Green I (Life Technologies, S-7563, Carlsbad, CA, USA), NEBNext High-Fidelity PCR Master Mix (
NEB
,
M0541S
), PCR-grade water and 10 μL of transposed DNA.
PCR
conditions were 5 min at 72 °C, 30 s at 98 °C and
(44)
The Mediator complex regulates enhancer-promoter interactions
Nature Structural & Molecular Biology
July 10, 2023
Shyam Ramasamy, Abrar Aljahani, Magdalena A. Karpinska, T. B. Ngoc Cao, Taras Velychko, J. Neos Cruz, Michael Lidschreiber, A. Marieke Oudelaar
samples were amplified using Nextera indexing primers and NEBNext High-Fidelity PCR Master Mix (
NEB
,
M0541
), with an initial 5-min extension step at 72 °C. A
real-time PCR
library amplification
kit (
KAPA
,
KK2701
)
(45)
PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection
Nature
July 1, 2023
Xu D, Jiang W, Wu L, Gaudet RG, Park ES, Su M, Cheppali SK, Cheemarla NR, Kumar P, Uchil PD,
et al
.
Protease Inhibitor Cocktail (11697498001) was obtained from Roche. High-Fidelity 2X PCR Master Mix (
M0541L
) was obtained from
NEB
. Plasmid constructs The following constructs were obtained from Addgene: pLenti-hACE2-hygro
(46)
Fanzor is a eukaryotic programmable RNA-guided endonuclease
Nature
June 28, 2023
Makoto Saito, Peiyu Xu, Guilhem Faure, Samantha Maguire, Soumya Kannan, Han Altae-Tran, Sam Vo, AnAn Desimone, Rhiannon K. Macrae, Feng Zhang
12.5 µl
PCR
reaction. Target sites were amplified with
NEBNext High-Fidelity 2X PCR Master Mix
(
NEB
,
M0541L
) under the following thermal cycling conditions: one cycle, 98 °C, 30 s; 15 cycles, 98 °C, 10 s, 65
(47)
RBBP4 is an epigenetic barrier for the induced transition of pluripotent stem cells into totipotent 2C-like cells
Nucleic Acids Research
June 23, 2023
Wangfang Ping, Yingliang Sheng, Gongcheng Hu, Hongxin Zhong, Yaoyi Li, YanJiang Liu, Wei Luo, Chenghong Yan, Yulin Wen, Xinxiu Wang,
et al.
phenol–chloroform and subjected to
PCR amplification
using NEBNext High-Fidelity 2× PCR Master Mix (
NEB
,
M0541S
) for 13 cycles. The libraries were size-selected with 1.2× AMpure XP beads (Beckman Coulter,
A63881
)
(48)
Selective binding of retrotransposons by ZFP352 facilitates the timely dissolution of totipotency network
Nature Communications
June 20, 2023
Zhengyi Li, Haiyan Xu, Jiaqun Li, Xiao Xu, Junjiao Wang, Danya Wu, Jiateng Zhang, Juan Liu, Ziwei Xue, Guankai Zhan,
et al.
using MinElute PCR Purification Kit (
QIAGEN
,
28006
), and amplified using 1xNEBnext PCR master mix (
NEB
,
M0541S
) using custom Nextera
PCR
primers 1 and 2. Upon
purification
with MinElute PCR Purification Kit (QIAGEN,
(49)
ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion
Nature Communications
June 19, 2023
Demis Menolfi, Brian J. Lee, Hanwen Zhang, Wenxia Jiang, Nicole E. Bowen, Yunyue Wang, Junfei Zhao, Antony Holmes, Steven Gershik, Raul Rabadan,
et al.
Illumina sequencing
adapters and a unique barcode for each sample, NEBNext High Fidelity Master Mix (
NEB
,
M0541L
), and 2.5 µg DNA for each reaction. The
PCR
products were purified using Zymospin V columns (Zymo Research,
(50)
Modularity and diversity of target selectors in Tn7 transposons
Molecular Cell
June 15, 2023
Guilhem Faure, Makoto Saito, Sean Benler, Iris Peng, Yuri I. Wolf, Jonathan Strecker, Han Altae-Tran, Edwin Neumann, David Li, Kira S. Makarova,
et al.
peptides, and recombinant proteins" ,,
NEBNext® High-Fidelity 2X PCR Master Mix
,
New England Biolabs
,
M0541
Q5® High-Fidelity 2X Master Mix
,
New England Biolabs
,
M0492
KAPA HiFi HotStart ReadyMix
,
Roche
,KK2602
KOD Hot Start DNA Polymerase
(51)
Comprehensive epigenomic profiling reveals the extent of disease-specific chromatin states and informs target discovery in ankylosing spondylitis
Cell Genomics
June 14, 2023
Andrew C. Brown, Carla J. Cohen, Olga Mielczarek, Gabriele Migliorini, Félicie Costantino, Alice Allcock, Connor Davidson, Katherine S. Elliott, Hai Fang, Alicia Lledó Lara,
et al.
purification kit,
Qiagen
,Cat#
28004
NEBNext High-Fidelity 2x PCR master mix
,
New England Biolabs
,Cat#
M0541S
/L NEBNext
DNA Library Prep
Master Mix Set,
New England Biolabs
,Cat#
E6040S
/L Herculase II Fusion Enzyme
(52)
Multiple repeat regions within mouse DUX recruit chromatin regulators to facilitate an embryonic gene expression program
bioRxiv
June 13, 2023
Christina M. Smith, Edward J. Grow, Sean C. Shadle, Bradley R. Cairns
PCR
was performed for 12 cycles using
NEBnext High-Fidelity 2X PCR Master Mix
(
New England Biolabs
,
M0541L
) (41). Samples were
1:1
.3 AMPure XP (Beckman Coulter) purified and sent for
sequencing
. All libraries
(53)
Impaired histone inheritance promotes tumor progression
Nature communications
June 10, 2023
Tian C, Zhou J, Li X, Gao Y, Wen Q, Kang X, Wang N, Yao Y, Jiang J, Song G,
et al
.
Cat.#D4013), and library
PCR amplification
was performed with NEBNext 2× PCR Master Mix (
NEB
, Cat.#
M0541S
) and standard Illumina Nextera indexing primers. The libraries were purified with Ampure XP beads at
(54)
Polycomb repressive complex 1.1 coordinates homeostatic and emergency myelopoiesis
eLife
June 2, 2023
Nakajima-Takagi Y, Oshima M, Takano J, Koide S, Itokawa N, Uemura S, Yamashita M, Andoh S, Aoyama K, Isshiki Y,
et al
.
Stain,Takara Bio,5761A, Commercial
assay
or kit,
NEBNext High Fidelity 2x PCR Master mix
,
New England BioLabs
,
M0541S
, Commercial
assay
or kit,Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1,10X Genomics,1000269, Commercial
(55)
Protocol for Bulk-ATAC sequencing in head and neck squamous cell carcinoma
STAR Protocols
June 1, 2023
Nana Chen, Manik Vohra, Srinivas Vinod Saladi
Green I,
Thermo Fisher Scientific
,
S7563
NEB next High Fidelity 2× PCR Master Mix,
New England Biolabs
,
M0541L
AMPure XP beads,
Beckman Coulter
,
A63880
Ethyl alcohol,Sigma,362808 JQ1,Sigma,SML1524 Antibiotic antimycotic
(56)
A protocol to study three-dimensional genome structure in individual mutant preimplantation mouse embryos
STAR Protocols
June 1, 2023
Daniel Gimenez-Llorente, Marta Portela, Ana Losada, Miguel Manzanares, Maria Jose Andreu
Beads,
Beckman Coulter
,Cat#
A63880
NEBNext® High-Fidelity 2X PCR Master Mix
,
New England Biolabs
,Cat#
M0541S
,, Critical commercial
assays
,Critical commercial
assays
,Critical commercial
assays
,,
NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1
(57)
Direct haplotype-resolved 5-base HiFi sequencing for genome-wide profiling of hypermethylation outliers in a rare disease cohort
Nature Communications
May 29, 2023
Warren A. Cheung, Adam F. Johnson, William J. Rowell, Emily Farrow, Richard Hall, Ana S. A. Cohen, John C. Means, Tricia N. Zion, Daniel M. Portik, Christopher T. Saunders,
et al.
“Appendix 1” of the Iso-Seq protocol from PacBio.
NEBNext High-Fidelity 2X PCR Master Mix
(
NEB
, Cat. No.
M0541S
) used in the
PCR
reamplification master mix and after reamplification, the cDNA was bead-cleaned following
(58)
Epigenome Editing Durability Varies Widely Across Cardiovascular Disease Target Genes
bioRxiv
May 20, 2023
Madelynn N. Whittaker, Lauren C. Testa, Aidan Quigley, Ishaan Jindal, Saúl V. Cortez-Alvarado, Ping Qu, Yifan Yang, Mohamad-Gabriel Alameh, Kiran Musunuru, Xiao Wang
≈15 ng of first-round
PCR product
as template and NEBNext Polymerase (
New England Biolabs
, catalog #
M0541S
). Final libraries were quantified via Qubit (Qiagen) and then sequenced on a MiSeq sequencer (Illumina).
Bisulfite sequencing
(59)
The Upstream Sequence Transcription Complex Dictates Nucleosome Positioning and Promoter Accessibility at piRNA Genes in the C. elegans Germ Line
bioRxiv
May 16, 2023
Nancy Sanchez, Lauren E Gonzalez, Valerie Reinke
according to the manufacturer’s instructions. DNA was pre-amplified with 2× NEBNext Master Mix (
NEB
M0541S
).
Library amplification
was assessed by
qPCR
on Applied Biosystems QuantStudio 3
real-time PCR system
.
(60)
linc-mipep and linc-wrb encode micropeptides that regulate chromatin accessibility in vertebrate-specific neural cells
eLife
May 16, 2023
Valerie A Tornini, Liyun Miao, Ho-Joon Lee, Timothy Gerson, Sarah E Dube, Valeria Schmidt, François Kroll, Yin Tang, Katherine Du, Manik Kuchroo,
et al.
MinElute Kit (
Qiagen
,
28004
). Libraries were prepared using NEBNext High-Fidelity 2 X PCR Master Mix (
NEB
,
M0541
) with the following conditions: 72 °C, 5 min; 98 °C, 30 s; 15 cycles of 98 °C, 10 s; 63 °C, 30 s; and
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NEBNext High-Fidelity 2X PCR Master Mix from New England Biolabs
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Pricing Info
Product Specs
Company Info
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