Welcome Guest
Sign In
Register
Products
Popular Categories
Antibody Search
ELISA Kit Search
Biomolecules
Flow Cytometers
Thermal Cyclers
Microscopes
Transfection Reagents
Featured Products
New Products
Articles
Resources
eBooks
Bench Tips
Tech Insights
Editorial Articles
News
Product Reviews
Future Lab
Multimedia
Biocompare Webinars
Immunotherapy
Reproducibility
Transformative Technologies
Podcasts
Tech Talks
Bench Tip Videos
All Videos and Webinars
Life Science Webinars
Write a Review
Antibodies
Kits/Reagents
Equipment
Services
Resources
Promotions
Events
AACR SPOTLIGHT
Register
Sign In
Biocompare
Products
Cell Biology
Biomolecules
Biomolecules
NEBNext High-Fidelity 2X PCR Master Mix
Supplier Images
NEBNext High-Fidelity 2X PCR Master Mix
NEBNext High-Fidelity 2X PCR Master Mix from New England Biolabs
Be the first to write a review!
Citations:
(491)
Supplier Page
Supplier Page from
New England Biolabs for
NEBNext High-Fidelity 2X PCR Master Mix
Get Pricing
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
NEBNext High-Fidelity 2X PCR Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0541S
Quantity
50 reactions
Applications
DNA Analysis, PCR
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (491)
(21)
A maternally programmed intergenerational mechanism enables male offspring to make piRNAs from Y-linked precursor RNAs in Drosophila
Nature Cell Biology
October 1, 2023
Zsolt G. Venkei, Ildar Gainetdinov, Ayca Bagci, Margaret R. Starostik, Charlotte P. Choi, Jaclyn M. Fingerhut, Peiwei Chen, Chiraag Balsara, Troy W. Whitfield, George W. Bell,
et al.
Ampure XP beads (
Beckman Coulter
,
A63880
), cDNA was
PCR
amplified using NEBNext High-Fidelity (
NEB
,
M0541
; 98 °C for 30 s; four cycles of: 98 °C for 10 s, 59 °C for 30 s, 72 °C for 12 s; six cycles of: 98 °C
(22)
Tracking cell-type-specific temporal dynamics in human and mouse brains
Cell
September 28, 2023
Lu Z, Zhang M, Lee J, Sziraki A, Anderson S, Zhang Z, Xu Z, Jiang W, Ge S, Nelson PT,
et al
.
(5′-CAAGCAGAAGACGGCATACGAGAT[i7]GTCTCGTGGGCTCGG-3′, IDT), and 20 μL
NEBNext High-Fidelity 2X PCR Master Mix
(
NEB
,
M0541L
) were added to each well.
Amplification
was carried out using the following program: 72°C for 5 min,
(23)
Methamphetamine-induced region-specific transcriptomic and epigenetic changes in the brain of male rats
Communications Biology
September 27, 2023
Benpeng Miao, Xiaoyun Xing, Viktoriia Bazylianska, Pamela Madden, Anna Moszczynska, Bo Zhang
prepared by amplifying for 9 cycles on a
PCR
machine with NEBNext High-Fidelity 2× PCR master mix (
NEB
,
M0541
). 2 × 75bp paired-end
sequencing
was run for all libraries on the Illumina NextSeq 500 platform. Raw
(24)
Radial glia promote microglial development through integrin αVβ8 -TGFβ1 signaling
bioRxiv
September 27, 2023
Gabriel L. McKinsey, Nicolas Santander, Xiaoming Zhang, Kilian Kleemann, Lauren Tran, Aditya Katewa, Kaylynn Conant, Matthew Barraza, Kian Waddell, Carlos Lizama,
et al.
(
Zymo research
, Cat.#
D4014
) and amplified using
NEBNext® High-Fidelity 2x PCR Master Mix
(
NEB
, Cat. #
M0541s
) for 9 cycles. The ATAC libraries were size selected using SPRIselect beads (Beckman Coulter, Cat.#B23317)
(25)
MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration
Signal Transduction and Targeted Therapy
September 15, 2023
Jinpeng Du, Wenwei Liao, Haichuan Wang, Guimin Hou, Min Liao, Lin Xu, Jiwei Huang, Kefei Yuan, Xiangzheng Chen, Yong Zeng
Qiagen
). Following
purification
, library fragments was amplificated in 1 × Nextera PCR master mix (Cat.
M0541S
;
NEB
) and Nextera
PCR
primers 1 and 2. Following
amplification
, a Qiagen MinElute Purification kit was
(26)
Fluid shear stress-modulated chromatin accessibility reveals the mechano-dependency of endothelial SMAD1/5-mediated gene transcription
iScience
September 15, 2023
Jerome Jatzlau, Paul-Lennard Mendez, Aybuge Altay, Lion Raaz, Yufei Zhang, Sophia Mähr, Akin Sesver, Maria Reichenbach, Stefan Mundlos, Martin Vingron,
et al.
Buenrostro 2015a73 were ligated to the DNA fragments (10μL + 10 μL H2O) using PCR Master Mix (
NEB
,
M0541L
) (25 μL) in a thermocycler (72°C, 5min; 98°C, 30 s; 5x (98°C, 10s; 63°C, 30 s; 72°C 1 min)).
qPCR
was
(27)
NF-κB subunits direct kinetically distinct transcriptional cascades in antigen receptor-activated B cells
Nature Immunology
September 1, 2023
Mingming Zhao, Prashant Chauhan, Cheryl A. Sherman, Amit Singh, Mary Kaileh, Krystyna Mazan-Mamczarz, Hongkai Ji, Jaimy Joy, Satabdi Nandi, Supriyo De,
et al.
Nextera
sequencing
primers and NEB high fidelity 2× PCR master mix for 12 cycles (
New England Biolabs
,
M0541
).
PCR
-amplified DNA was purified using MinElute columns and sequenced using the Illumina NextSeq sequencer
(28)
The SWI/SNF complex member SMARCB1 supports lineage fidelity in kidney cancer
iScience
August 18, 2023
Ludovic Wesolowski, Jianfeng Ge, Leticia Castillon, Debora Sesia, Anna Dyas, Shoko Hirosue, Veronica Caraffini, Anne Y. Warren, Paulo Rodrigues, Giovanni Ciriello,
et al.
amplified for a total of 8-12 cycles using custom Nextera
PCR
primers and NEBNext PCR master mix (
NEB
M0541S
). The amplified libraries were purified using Agencourt AMPureXP reagents (A63880), profiled on the
(29)
ATRX guards against aberrant differentiation in mesenchymal progenitor cells
bioRxiv
August 8, 2023
Yan Fang, Douglas Barrows, Yakshi Dabas, Thomas S Carroll, William D. Tap, Benjamin A. Nacev
collected for Omni-
ATAC-seq
.
Amplification
was performed with NEBNext High-Fidelity 2× PCR Master Mix (
NEB
,
M0541s
) and Nextera
PCR
Primers (8 cycles). The sequences of
PCR
primers with index adapters are listed in
(30)
LRP8‐mediated selenocysteine uptake is a targetable vulnerability in MYCN‐amplified neuroblastoma
EMBO Molecular Medicine
August 7, 2023
Hamed Alborzinia, Zhiyi Chen, Umut Yildiz, Florencio Porto Freitas, Felix C E Vogel, Julianna Patricia Varga, Jasmin Batani, Christoph Bartenhagen, Werner Schmitz, Gabriele Büchel,
et al.
740424).
Sequencing
libraries were prepared using the NEBNext 2× High‐Fidelity Master Mix (
NEB
, Cat. No.
M0541L
) with
amplification
primers partially complementary to the lentiviral gRNA backbone with overhangs introducing
(31)
The master growth regulator DELLA binding to histone H2A is essential for DELLA-mediated global transcription regulation
Nature Plants
August 3, 2023
Xu Huang, Hao Tian, Jeongmoo Park, Dong-Ha Oh, Jianhong Hu, Rodolfo Zentella, Hong Qiao, Maheshi Dassanayake, Tai-Ping Sun
buffer. The purified tagmented DNA was first amplified using Next High-Fidelity 2X PCR Master Mix (
NEB
,
M0541S
) in 50 μL reactions, and a distinct barcoded Primer 2 was used for each library (Supplementary Table
(32)
HDAC1 and HDAC6 are essential for driving growth in IDH1 mutant glioma
Sci Rep
August 1, 2023
Matthew C. Garrett, Rebecca Albano, Troy Carnwath, Lubayna Elahi, Catherine A. Behrmann, Merissa Pemberton, Daniel Woo, Eric O'Brien, Brett VanCauwenbergh, John Perentesis,
et al.
Following
purification
, transposed DNA was mixed with NEBNext high-Fidelity 2× PCR Master Mix (
NEB
, #
M0541S
), AD1_noMX and AD2.1–2.16 barcoded primers, and nuclease free water. Samples were amplified for 12 cycles.
(33)
The contributions of DNA accessibility and transcription factor occupancy to enhancer activity during cellular differentiation
bioRxiv
July 27, 2023
Trevor Long, Tapas Bhattacharyya, Andrea Repele, Madison Naylor, Sunil Nooti, Shawn Krueger, Manu
amplified in a 50μL
PCR
reaction using
NEBNext High-Fidelity 2x PCR Master Mix
(
New England BioLabs
,
M0541S
) for 5 cycles after a 5 min elongation step. Primers included adaptors for
sequencing
and were barcoded
(34)
FOXA2 controls the anti-oxidant response in FH-deficient cells
Cell Reports
July 25, 2023
Connor Rogerson, Marco Sciacovelli, Lucas A. Maddalena, Andromachi Pouikli, Marc Segarra-Mondejar, Lorea Valcarcel-Jimenez, Christina Schmidt, Ming Yang, Elena Ivanova, Joshua Kent,
et al.
HiFi Hot Start DNA polymerase,KAPA Biosystems,KK2500
NEBNext® High-Fidelity 2X PCR Master Mix
,
NEB
,
M0541S
Ampure XP beads,
Beckman Coulter
,
A63880
5x siRNA buffer,
Horizon
,
B-002000-UB-100
Lipofectamine RNAiMAX,
ThermoFisher
,
13778075
Revert™
(35)
Coordinated inheritance of extrachromosomal DNA species in human cancer cells
bioRxiv
July 25, 2023
King L. Hung, Matthew G. Jones, Ivy Tsz-Lo Wong, Joshua T. Lange, Jens Luebeck, Elisa Scanu, Britney Jiayu He, Lotte Brückner, Rui Li, Rocío Chamorro González,
et al.
were generated by 5–7 rounds of
PCR amplification
using NEBNext High-Fidelity 2× PCR Master Mix (
NEB
,
M0541L
), purified using SPRIselect reagent kit (Beckman Coulter, B23317) with double size selection (0.8× right,
(36)
Systematic mapping and modeling of 3D enhancer-promoter interactions in early mouse embryonic lineages reveal regulatory principles that determine the levels and cell-type specificity of gene expression
bioRxiv
July 25, 2023
Dylan Murphy, Eralda Salataj, Dafne Campigli Di Giammartino, Javier Rodriguez-Hernaez, Andreas Kloetgen, Vidur Garg, Erin Char, Christopher M. Uyehara, Ly-sha Ee, UkJin Lee,
et al.
Kit (D4014).
ATAC-seq
libraries were prepared using
NEBNext High-Fidelity 2X PCR Master Mix
(
NEB
, #
M0541
), a uniquely barcoded primer per sample, and a universal primer. Samples were first subjected to 5 cycles
(37)
Proximity-dependent recruitment of Polycomb repressive complexes by the lncRNA Airn
Cell reports
July 25, 2023
Braceros AK, Schertzer MD, Omer A, Trotman JB, Davis ES, Dowen JM, Phanstiel DH, Aiden EL, Calabrese JM.
ligation
by Quick ligase (
NEB
, cat #:
M2200S
), and
NEBNext High-Fidelity 2X PCR Master Mix
(
NEB
, cat #:
M0541S
). All DNA size-selection
purification
steps were performed with AMPure XP beads (
Beckman Coulter
, cat
(38)
The BAF chromatin complex component SMARCC1 does not mediate GLI transcriptional repression of Hedgehog target genes in limb buds
bioRxiv
July 21, 2023
Janani Ramachandran, Wanlu Chen, Rachel K. Lex, Kathryn E. Windsor, Hyunji Lee, Tingchang Wang, Weiqiang Zhou, Hongkai Ji, Steven A. Vokes
37°C, shaking gently. Libraries were generated using the NEB High-Fidelity 2x Mix (
New England Biolabs
M0541S
),
PCR
amplified for 11 cycles and cleaned up using SparQ PureMag beads (QuantaBio 95196-060). Libraries
(39)
De novo purine metabolism is a metabolic vulnerability of cancers with low p16 expression
bioRxiv
July 20, 2023
Naveen Kumar Tangudu, Raquel Buj, Jiefei Wang, Aidan R. Cole, Apoorva Uboveja, Richard Fang, Amandine Amalric, Maureen A. Lyons, Uma R. Chandran, Katherine M. Aird
Addgene 52961) by
PCR amplification
using NEB Next High-Fidelity PCR Master Mix (
New England Biolabs
,
M0541S
). The
PCR product
was digested with
Esp3I
(
BsmBI
) (Fisher Scientific, FERFD0454) and ligated into the
(40)
High throughput PRIME editing screens identify functional DNA variants in the human genome
bioRxiv
July 18, 2023
Xingjie Ren, Han Yang, Jovia L. Nierenberg, Yifan Sun, Jiawen Chen, Cooper Beaman, Thu Pham, Mai Nobuhara, Maya Asami Takagi, Vivek Narayan,
et al.
were performed using 0.01 fmol of pooled oligos with NEBNext High-Fidelity 2× PCR Master Mix (
NEB
,
M0541S
). Each 20 μl PCR mix was combined with 40 μl of oil-surfactant mixture (containing 4.5 % Span 80 (v/v),
Page
<<
<
1
2
3
4
5
>
>>
Reviews
Be the First to Write a Review!
NEBNext High-Fidelity 2X PCR Master Mix from New England Biolabs
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
Back To Top