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Q5 Hot Start High-Fidelity DNA Polymerase
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Q5 Hot Start High-Fidelity DNA Polymerase
Q5 Hot Start High-Fidelity DNA Polymerase from New England Biolabs
(1)
Write a review
Citations:
(173)
Supplier Page
Supplier Page from
New England Biolabs for
Q5 Hot Start High-Fidelity DNA Polymerase
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Q5 Hot Start High-Fidelity DNA Polymerase
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0493L
Quantity
500 units
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Type
DNA Polymerase
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (173)
(61)
Distinct MUNC lncRNA structural domains regulate transcription of different promyogenic factors
Cell reports
February 15, 2022
Przanowska RK, Weidmann CA, Saha S, Cichewicz MA, Jensen KN, Przanowski P, Irving PS, Janes KA, Guertin MJ, Weeks KM,
et al
.
M3634 Betaine,
Millipore Sigma
,
B0300
-1VL Q5 Hot-start high Fidelty DNA Polymerase,
New England Biolabs
,Cat#
M0493
Puromycin,Sigma,Cat# P9620 Hygromycin,
Life technologies
,Cat#
10687-010
Lipofectamine 3000
,
Life Technologies
,Cat#
(62)
Widespread discrepancy in Nnt genotypes and genetic backgrounds complicates granzyme A and other knockout mouse studies
eLife
February 4, 2022
Daniel J Rawle, Thuy T Le, Troy Dumenil, Cameron Bishop, Kexin Yan, Eri Nakayama, Phillip I Bird, Andreas Suhrbier
,
iScript cDNA Synthesis Kit
,
Bio-Rad
,Cat#
1708890
, "Commercial
assay
, kit",
Q5 Hot Start High-Fidelity DNA Polymerase
,
NEB
,Cat#
M0493S
,Enzyme Other,Illumina HiSeq 2000 Sequencer,Illumina,RRID:SCR_010233,
Sequencing
platform Other,NextSeq
(63)
A Case Study of the Morphological and Molecular Variation within a Ciliate Genus: Taxonomic Descriptions of Three Dysteria Species (Ciliophora, Cyrtophoria), with the Establishment of a New Species
International Journal of Molecular Sciences
February 3, 2022
Xuetong Zhao, Hui Zhang, Qianqian Zhang, Zhishuai Qu, Alan Warren, Di Wu, Xiangrui Chen
of
amplification
errors, a
Q5 Hot Start High-Fidelity DNA Polymerase
(
New England Biolabs
Co., Ltd.,
M0493
, Beijing) was used. The
polymerase chain reaction (PCR
) conditions used in the
amplification
were as
(64)
Multiple expansions of globally uncommon SARS-CoV-2 lineages in Nigeria
Nature communications
February 3, 2022
Ozer EA, Simons LM, Adewumi OM, Fowotade AA, Omoruyi EC, Adeniji JA, Olayinka OA, Dean TJ, Zayas J, Bhimalli PP,
et al
.
(version 3 release).
PCR amplification
was carried out using Q5 Hot Start HF
Taq Polymerase
(
NEB
, cat. no.
M0493L
) with 5 μl of cDNA in a 25 μl reaction volume. A two-step
PCR
program was used with an initial step
(65)
Isothermal Amplification with a Target-Mimicking Internal Control and Quantitative Lateral Flow Readout for Rapid HIV Viral Load Testing in Low-Resource Settings
Analytical Chemistry
January 18, 2022
Ian T. Hull, Enos C. Kline, Gaurav K. Gulati, Jack Henry Kotnik, Nuttada Panpradist, Kamal G. Shah, Qin Wang, Lisa Frenkel, James Lai, Joanne Stekler,
et al.
gBlocks using M13
PCR
primers and Q5 HotStart High-Fidelity DNA Polymerase (New England BioLabs (
NEB
)
M0493
). Each 50µL
PCR
reaction contained 1x Q5 Reaction Buffer, 200µM each dNTPs, 500nM each M13 primer, 1U
(66)
Discovery of ultrapotent broadly neutralizing antibodies from SARS-CoV-2 elite neutralizers
Cell Host & Microbe
January 12, 2022
Kanika Vanshylla et al.
Platinum™ Taq Green Hot Start DNA Polymerase
,
Thermo Fisher
,Catalog#
11966034
Q5 Hot Start High Fidelity DNA Polymerase
,
NEB
,Catalog#
M0493L
"Branched Polyethylenimine, 25 kDa",Sigma-Aldrich,Catalog# 408727 Puromycin,Sigma,Catalog# P4512 Doxycyclin,Sigma,Catalog#
(67)
Allele-specific endogenous tagging and quantitative analysis of β-catenin in colorectal cancer cells
eLife
January 11, 2022
Ambrosi G, Voloshanenko O, Eckert AF, Kranz D, Nienhaus GU, Boutros M.
Master Mix
QPCR
,Roche,4887301001, Other,
Q5 Hot Start High-Fidelity DNA Polymerase
,
New England Biolabs
,
M0493S
, Other,dNTP Set 100 mM,VWR/Fermentas/
Thermo Fisher Scientific
,
R0182
, Commercial
assay
or kit,Light
(68)
Deciphering the physiological response of
Escherichia coli
under high ATP demand
Molecular Systems Biology
December 20, 2021
Simon Boecker, Giulia Slaviero, Thorben Schramm, Witold Szymanski, Ralf Steuer, Hannes Link, Steffen Klamt
polymerase chain reaction (PCR
) using the
Q5 Hot Start High‐Fidelity DNA Polymerase
(
New England Biolabs
, #
M0493L
) and the primer pair atpAGD_mono_fw/atpAGD_mono_rv as described in (Boecker et al, 2019). To construct
(69)
Modelling of primary ciliary dyskinesia using patient-derived airway organoids
EMBO reports
December 6, 2021
van der Vaart J, Böttinger L, Geurts MH, van de Wetering WJ, Knoops K, Sachs N, Begthel H, Korving J, Lopez-Iglesias C, Peters PJ,
et al
.
performed using standard
Q5® Hot Start High‐Fidelity DNA Polymerase
protocol (
New England Biolabs
;
M0493
).
PCR
products were purified using NucleoSpin Gel and PCR Clean‐up kit (Bioke; 740609) and Sanger sequenced
(70)
New Data Define the Molecular Phylogeny and Taxonomy of Four Freshwater Suctorian Ciliates With Redefinition of Two Families Heliophryidae and Cyclophryidae (Ciliophora, Phyllopharyngea, Suctoria)
Frontiers in Microbiology
December 3, 2021
Mingzhen Ma, Yuqing Li, Qingxiang Yuan, Xuetong Zhao, Khaled A. S. Al-Rasheid, Jie Huang, Honggang Ma, Xiangrui Chen
amplification
of the SSU rDNA was performed using
Q5®Hot Start High-Fidelity DNA Polymerase
(
NEB
Co., Ltd.,
M0493
, Beijing) with the universal eukaryotic primers 82F (5′ GAA ACT GCG AAT GGC TC 3′) and 18S-R (5′ TGA
(71)
In vivo
architecture of the telomerase RNA catalytic core in
Trypanosoma brucei
Nucleic Acids Research
December 2, 2021
Abhishek Dey, Anais Monroy-Eklund, Kaitlin Klotz, Arpita Saha, Justin Davis, Bibo Li, Alain Laederach, Kausik Chakrabarti
(eCR4-CR5) domain (Supplementary Table S1) and
PCR
amplified these regions using
NEB
Q5 HotStart polymerase (
M0493L
). A secondary
PCR
was performed to introduce TrueSeq barcodes (55). Quantification of the libraries
(72)
Programmable human histone phosphorylation and gene activation using a CRISPR/Cas9-based chromatin kinase
Nature Communications
December 1, 2021
Jing Li, Barun Mahata, Mario Escobar, Jacob Goell, Kaiyuan Wang, Pranav Khemka, Isaac B. Hilton
as a template across eight 100 μl
PCR reactions
to amplify gRNAs using Q5 hot start polymerase (
NEB
,
M0493L
).
Amplification
was carried out as per manufacturer’s instructions, using 25 cycles at an annealing
(73)
Improved library preparation protocols for amplicon sequencing-based noninvasive fetal genotyping for RHD-positive D antigen-negative alleles
BMC Research Notes
December 1, 2021
Asuka Hori et al.
genomic DNA of two individuals (or cfDNA) using 0.5 unit of
Q5 Hot Start High-Fidelity DNA Polymerase
(
M0493
,
NEB
) according to the manufacturer’s instruction in a 25 µL reaction at the following final concentrations:
(74)
Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli
mBio
October 26, 2021
Melanie R. Silvis et al.
treatment. sgRNA spacer sequences were amplified from gDNA using Q5 polymerase (
New England Biolabs
number
M0493S
) for 14 cycles using custom primers containing TruSeq adapters and indices, followed by gel
purification
(75)
Germline polymorphisms and alternative splicing of human immunoglobulin light chain genes
iScience
October 22, 2021
Ivana Mikocziova, Ayelet Peres, Moriah Gidoni, Victor Greiff, Gur Yaari, Ludvig M. Sollid
and recombinant proteins" ,,
Q5® Hot Start High-Fidelity DNA Polymerase
,
New England Biolabs
,Cat#:
M0493S
"
DNA Polymerase I, Large (Klenow) Fragment
",
New England Biolabs
,Cat#:
M0210S
NEBNext® dA-Tailing Reaction Buffer
(76)
Circular RNAs in Hedgehog Signaling Activation and Hedgehog-Mediated Medulloblastoma Tumors
Cancers
October 13, 2021
Azatyan A, Zhang S, Darabi A, Siesjö P, Wang T, Zaphiropoulos PG.
in the next section and
PCR reactions
were performed with
Q5 Hot Start High-Fidelity DNA Polymerase
(
M0493S
,
NEB
, Ipswich, MA, USA) using 1000 ng template cDNA, in a 50 uL reaction mix under the following conditions:
(77)
Detection of low-frequency DNA variants by targeted sequencing of the Watson and Crick strands
Nature Biotechnology
October 1, 2021
Joshua D. Cohen et al.
supplemented with an additional 0.5 U of
Q5 Hot Start High-Fidelity DNA Polymerase
(
New England BioLabs
,
M0493L
), 1 μl of 10 mM dNTPs (
New England BioLabs
,
N0447L
) and 0.4 μl of 25 mM MgCl2 solution (
New England BioLabs
(78)
Genome-wide functional screen of 3′UTR variants uncovers causal variants for human disease and evolution
Cell
September 30, 2021
Dustin Griesemer et al.
containing 5.7 ng of oligo, 25 μL of Q5 NEBNext MasterMix (
NEB
,
M0541S
), 1 unit Q5 HotStart polymerase (
NEB
,
M0493S
), 0.5 μM oligo_BAR_Bmt_F and oligo_pmir_R_min primers, and 20 μg
BSA
(
NEB
, B9000) (Supplementary Table
(79)
Receptor Switching in Newly Evolved Adeno-associated Viruses
Journal of Virology
September 9, 2021
L. Patrick Havlik, Anshuman Das, Mario Mietzsch, Daniel K. Oh, Jonathan Ark, Robert McKenna, Mavis Agbandje-McKenna, Aravind Asokan
AAV840 integrin mutants were created by
site-directed mutagenesis
using Q5 Hot Start Polymerase (
NEB
,
M0493S
). AAV vector production,
purification
, and quantification. Recombinant AAV vectors were produced by
(80)
A novel mechanism of enhanced transcription activity and fidelity for influenza A viral RNA-dependent RNA polymerase
Nucleic Acids Research
September 7, 2021
Xinzhou Xu, Lu Zhang, Julie Tung Sem Chu, Yuqing Wang, Alex Wing Hong Chin, Tin Hang Chong, Zixi Dai, Leo Lit Man Poon, Peter Pak-Hang Cheung, Xuhui Huang
the MBTuni-12 primer and amplified for 22 cycles using
Q5 Hot Start High-Fidelity DNA Polymerase
(
NEB
M0493
) using the MBTuni-12 and MBTuni-13 primers. The
NGS
collection was constructed using 2 ng DNA from two
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Reviews
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(1)
4/9/2015
Hi Fidelity Polymerase
Benjamin Gerovac
I used this polymerase while performing PCR to subclone a gene fragment into a viral vector so I could transduce primary epithelial cells. Upon sequencing, this polymerase gave a PCR product of the ...
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Q5 Hot Start High-Fidelity DNA Polymerase from New England Biolabs
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