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Bst2.0 WarmStartDNA Polymerase
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Bst2.0 WarmStartDNA Polymerase
Bst2.0 WarmStartDNA Polymerase from New England Biolabs
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Citations:
(36)
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New England Biolabs for
Bst2.0 WarmStartDNA Polymerase
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Company Info
Citations
Reviews
Product Specs
Item
Bst2.0 WarmStartDNA Polymerase
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0538M
Quantity
8000 units
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Type
DNA Polymerase
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (36)
(1)
Simultaneous and rapid colorimetric detection of distinct miRNAs using Split-LAMP
Frontiers in Bioengineering and Biotechnology
November 2, 2023
Yi Jing Chua, Steven Poh Chuen Sim, Medha Shridharan, Yiqi Seow
2.2 Primers (Integrated DNA Technologies),As stated in Section 2.2 "WarmStart Bst 2.0 (catalog number
M0538M
,
New England Biolabs
)",6.4 U Components in a LAMP reaction.
(2)
Loop mediated isothermal amplification as a molecular diagnostic assay: Application and evaluation for detection of Enterohaemorrhagic Escherichia coli (O157:H7)
Practical Laboratory Medicine
November 1, 2023
Degisew Yinur, Biniam Moges, Aliyi Hassen, Tesfaye Sisay Tessema
MgSO4, 2.5 μl of 10X Bst ThermoPol reaction buffer, 8 U of Bst 2.0 DNA polymerase (
NEW ENGLAND BioLABS
#
M0538
), ddH2O, and 2 μL of target genomic DNA. The reaction mixture was incubated at 65 °C for 60 min in a
(3)
The extinct Sicilian wolf shows a complex history of isolation and admixture with ancient dogs
iScience
August 18, 2023
Marta Maria Ciucani, Jazmín Ramos-Madrigal, Germán Hernández-Alonso, Alberto Carmagnini, Sabhrina Gita Aninta, Xin Sun, Camilla Hjorth Scharff-Olsen, Liam Thomas Lanigan, Ilaria Fracasso, Cecilie G. Clausen,
et al.
T4 DNA Polymerase
,
New England Biolabs
Inc.,Cat#
M0203S
"BSt 2,0 warmstart polymerase",
New England Biolabs
Inc.,Cat#
M0538S
Phusion® High-Fidelity PCR Master Mix with HF buffer
,
New England Biolabs
Inc.,Cat#
M0531S
DNeasy® Blood
(4)
Viral load quantitation at the point-of-care with shaken digital droplet RT-LAMP
Lab on a Chip
July 25, 2023
Daniel W. Weisgerber, Krzysztof Langer, Venice Servellita, Peng Xu, Charles Y. Chiu, Adam R. Abate
B1003S
), 1.875 mM dNTPs (
ThermoFisher Scientific
R1122
), 8 U Bst 2.0 WarmStart DNA polymerase (
NEB
M0538L
), 100 U
SuperScript III reverse transcriptase
(
ThermoFisher Scientific
18 080 093) and the LAMP primer
(5)
Ultrasensitive Isothermal Detection of SARS-CoV-2 Based on Self-Priming Hairpin-Utilized Amplification of the G-Rich Sequence
Analytical Chemistry
December 20, 2022
Yan Li, Hansol Kim, Yong Ju, Yeonkyung Park, Taejoon Kang, Dongeun Yong, Hyun Gyu Park
the Korea Bank for Pathogenic
Viruses
(KBPV, Seoul, Korea). Bst 2.0 WarmStart DNA polymerase (Bst,
M0538S
),
Nt.BstNBI
(
R0607S
),
NEBuffer 3.1
(
B7203S
), ThermoPol reaction buffer (
B9004S
), and
deoxynucleotide (dNTP) solution mix
(6)
Development of an optimized colorimetric RT-LAMP for SARS-CoV-2 assay with enhanced procedure controls for remote diagnostics
Scientific Reports
December 11, 2022
Bruna Winkert Raddatz, Edson Yu Sin Kim, Louise Matiê Imamura, Gisleine Jarenko Steil, Erika Bergamo Santiago, Santiago Pedro Timm Soares, Victor Henrique Alves Ribeiro, Bernardo Montesanti Machado de Almeida, Sergio Renato Rogal, Marcus Vinícius Mazega Figueredo
configurations. Materials and methods Reagents and equipment Bst 2.0 WarmStart® DNA Polymerase (
M0538
),
Antarctic Thermolabile UDG
(
M0372
),
WarmStart® RTx Reverse Transcriptase
(M0380), as well as dNTPs
(7)
METTL14 is required for exercise-induced cardiac hypertrophy and protects against myocardial ischemia-reperfusion injury
Nature communications
November 9, 2022
Wang L, Wang J, Yu P, Feng J, Xu GE, Zhao X, Wang T, Lehmann HI, Li G, Sluijter JPG,
et al
.
1 min, 50 °C for 1 min, 40 °C for 6 min)53,71. Then, 0.1 U Bst 2.0 WarmStart DNA polymerase (
NEB
, #
M0538S
), 5U
SplintR ligase
(
NEB
, #
M0375S
), 50μΜ dTTP (Diamond, #B110051-0250) and 1 mM ATP (
NEB
, #
P0756S
) was
(8)
Polony gels enable amplifiable DNA stamping and spatial transcriptomics of chronic pain
Cell
November 8, 2022
Xiaonan Fu, Li Sun, Runze Dong, Jane Y. Chen, Runglawan Silakit, Logan F. Condon, Yiing Lin, Shin Lin, Richard D. Palmiter, Liangcai Gu
buffer,
Qiagen
,Cat#
1034963
BSA
,
New England Biolabs
,Cat#M9000S Bst 2.0 WarmStart DNA polymerase,
New England Biolabs
,Cat#
M0538L
Ultra II Q5 master mix,
New England Biolabs
,Cat#
M0544L
Thermolabile exonuclease I
,
New England Biolabs
,Cat#
M0568L
Q5
(9)
A method of large DNA fragment enrichment for nanopore sequencing in region 22q11.2
Frontiers in Genetics
October 31, 2022
Yu-Qing Lei, Liang-Pu Xu, Hua Cao, Xin-Rui Wang
quickly placed on ice. After adding eight units of Bst 2.0 WarmStart DNA polymerase (
NEB
, Cat. no.
M0538
), the samples were incubated at 60°C for 10 min and 80°C for 20 min. Ten units of
T7 exonuclease
(
NEB
,
(10)
Development and Clinical Validation of RT-LAMP-Based Lateral-Flow Devices and Electrochemical Sensor for Detecting Multigene Targets in SARS-CoV-2
International Journal of Molecular Sciences
October 28, 2022
Apoorva Saxena, Pawankumar Rai, Srishti Mehrotra, Samiya Baby, Suman Singh, Vikas Srivastava, Smriti Priya, Sandeep K. Sharma
buffer, 6 mM of MgSO4, 1.4 mM of dNTPs, 800 mM of betaine, 8 U of Bst 2.0 WarmStart DNA polymerase (#
M0538
,
New England Biolabs
, Ipswich, MA, USA), 1.6 M of forward inner primer (FIP) and backward inner primer
(11)
Point-of-care testing for COVID-19 using SHERLOCK diagnostics
medRxiv
October 1, 2022
Julia Joung et al.
freeze-thaw cycles. For Step (2) - STOPCovid detection reaction: Bst 2.0 WarmStart® DNA Polymerase (
M0538L
), New England BioLabsWarmStart® RTx Reverse Transcriptase (
M0380L
), New England BioLabs10X
Isothermal Amplification
(12)
Functional inference of gene regulation using single-cell multi-omics
Cell Genomics
September 1, 2022
Vinay K. Kartha et al.
whilst minimizing the number of droplets with multiple beads to <10%. Furthermore, Bst 2.0 WarmStart (
M0538S
,
NEB
) was added to the droplet mix to perform temperature activated second strand synthesis in droplets. scATAC-seq
(13)
Stimulation of adaptive gene amplification by origin firing under replication fork constraint
Nucleic Acids Research
January 25, 2022
Alex J Whale, Michelle King, Ryan M Hull, Felix Krueger, Jonathan Houseley
Isothermal amplification
buffer (NEB), 3 μl 100 mM MgSO4, 2 μl 10 mM dNTPs and 1 μl Bst 2 WarmStart DNA polymerase (
NEB
M0538S
) was added and sample incubated 30 min at 65°C before precipitation with 12.5 μl 10 M NH4OAc, 1 μl GlycoBlue,
(14)
A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis
Nature Communications
December 1, 2021
Guanhua Xun, Stephan Thomas Lane, Vassily Andrew Petrov, Brandon Elliott Pepa, Huimin Zhao
(www.neb.com/protocols/2014/10/09/typical-rt-lamp-protocol) with following changes: WarmStart® Bst 2.0 (Cat#
M0538L
,
NEB
) with the final concentration of 320 units/mL, WarmStart® RTx (Cat#
M0380L
,
NEB
) with the final
(15)
Single-copy detection of somatic variants from solid and liquid biopsy
Scientific Reports
December 1, 2021
Ana-Luisa Silva, Paulina Klaudyna Powalowska, Magdalena Stolarek, Eleanor Ruth Gray, Rebecca Natalie Palmer, Bram Herman, Cameron Alexander Frayling, Barnaby William Balmforth
was prepared by mixing 3 µL Thermopol buffer (B9004, New England Biolabs), 0.4 µL BST 2.0 WarmStart (
M0538
,
New England Biolabs
), 1.2 µL dNTPs mix (10 mM stock, Promega), 1.13 µL Syto82 dye (
S11363
,
Thermo Fisher Scientific
(16)
Kouprey (Bos sauveli) genomes unveil polytomic origin of wild Asian Bos
iScience
November 19, 2021
Mikkel-Holger S. Sinding, Marta M. Ciucani, Jazmín Ramos-Madrigal, Alberto Carmagnini, Jacob Agerbo Rasmussen, Shaohong Feng, Guangji Chen, Filipe G. Vieira, Valeria Mattiangeli, Rajinder K. Ganjoo,
et al.
T4 DNA Polymerase
,
New England Biolabs
Inc.,Cat#
M0203L
"BSt 2,0 warmstart polymerase",
New England Biolabs
Inc.,Cat#
M0538L
,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,
(17)
A mapping platform for mitotic crossover by single-cell multi-omics
Methods in Enzymology
November 15, 2021
Peter Chovanec, Yi Yin
(FisherScientific, cat. no. 07-678-003)Bst 2.0 WarmStart® DNA Polymerase (
New England Biolabs
, cat. no.
M0538
)HiScribe T7 Quick High Yield
RNA Synthesis
Kit (
New England Biolabs
, cat. no.
E2050S
)SUPERase•In™ RNase
(18)
A chemical-enhanced system for CRISPR-Based nucleic acid detection
Biosensors and Bioelectronics
November 15, 2021
Zihan Li, Wenchang Zhao, Shixin Ma, Zexu Li, Yingjia Yao, Teng Fei
with 1 μL 10x
isothermal amplification
buffer, 1.4 mM of dNTPs, 6.5 mM MgSO4, 3.2 U Bst 2.0 (
NEB
, #
M0538S
), 3 U
WarmStart RTx Reverse Transcriptase
(
NEB
, #
M0380S
), 0.2 μM F3/B3 primers, 1.6 μM FIP/BIP primers,
(19)
A Novel Miniature CRISPR-Cas13 System for SARS-CoV-2 Diagnostics
ACS Synthetic Biology
October 15, 2021
Ahmed Mahas, Qiaochu Wang, Tin Marsic, Magdy M. Mahfouz
2 mM MgSO4, and 0.1% Tween 20) (B0537, NEB); 1.4 mM dNTPs; 8 U of Bst2.0 WarmStart DNA Polymerase (
M0538
,
NEB
); 7.5 U of
WarmStart RTx Reverse Transcriptase
(M0380,
NEB
); and 6 mM MgSO4 (B1003, NEB) in 25
(20)
Intrinsic signal amplification by type III CRISPR-Cas systems provides a sequence-specific SARS-CoV-2 diagnostic
Cell Reports Medicine
June 15, 2021
Andrew Santiago-Frangos, Laina N. Hall, Anna Nemudraia, Artem Nemudryi, Pushya Krishna, Tanner Wiegand, Royce A. Wilkinson, Deann T. Snyder, Jodi F. Hedges, Calvin Cicha,
et al.
RNase Inhibitor,
NEB
,Cat#
M0314
Calcein,MP Biomedical,Cat# 02190167-CF WarmStart Bst 2.0,
NEB
,Cat#
M0538L
WarmStart RTx Reverse Transcriptase
,
NEB
,Cat#
M0380L
Hi-T7 RNA Polymerase
,
NEB
,Cat# M0658 TaqPath
1-Step RT-qPCR
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Bst2.0 WarmStartDNA Polymerase from New England Biolabs
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Pricing Info
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Company Info
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