Fig 2: Western blot analysis of WIF-1, BIM, Bax, HOXA9, Wnt, ß-catenin, Survivin, Cyclin D1, c-Myc, Mcl-1, Bcl-xL, and Snail expression in OS compared to normal tissue(A) Histogram showing the relative expression of WIF-1, BIM, Bax, HOXA9, Wnt, ß-catenin, Survivin, Cyclin D1, c-Myc, Mcl-1, Bcl-xL, and Snail in OS compared to normal tissue; (B) Western blot images of protein expression in OS compared to normal tissue; *P < 0.05.

Fig 3: RT-qPCR analysis of miR-182, WIF-1, BIM, Bax, HOXA9, Wnt, ß-catenin, Survivin, Cyclin D1, c-Myc, Mcl-1, Bcl-xL, and Snail expression in OS compared to normal tissue*P < 0.05.

Fig 4: RT-qPCR analysis of miR-182, WIF-1, ß-catenin, BIM, Bax, HOXA9, Survivin, Cyclin D1, c-Myc, Mcl-1, Bcl-xL, and Snail expression following transfection(A) Histograms showing the relative expression of miR-182, HOXA9, ß-catenin, and WIF-1 in the six groups; (B) Histograms showing the relative expression of Survivin, c-Myc, Cyclin D1, and Snail in the six groups; (C) Histograms showing the relative expression of BIM, Mcl-1, Bcl-xL, and Bax in the six groups. *P < 0.05, compared to the NC group.

Fig 5: Comparison of xenograft tumor size and growth rates in the six groups(A) Cell growth; (B) Tumor size; (C) Western blot analysis of HOXA9, WIF-1, and ß-catenin protein expression in the six groups; (D) Histograms showing the relative expression of HOXA9, WIF-1, and ß-catenin in tumor tissue from nude mice in the six experimental groups; (E) Histograms showing the relative expression of c-Myc, Survivin, Snail, and Cyclin D1 in tumor tissue from mice in the six groups; (F) Histograms showing the relative expression of BIM, Mcl-1, Bcl-xL, and Bax in tumor tissue from mice in the six groups; *P < 0.05, compared to the blank and NC groups.