APC Annexin V 640919 from BioLegend

Product Specs
ItemAPC Annexin V
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number640919
Quantity25 tests
ApplicationsFlow Cytometry (FC)
Conjugate/TagAPC
NCBI Full Gene Nameannexin A5
NCBI Gene AliasesANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
TargetAnnexin V
SpeciesHuman, Mouse, Rat

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) The NCOR-HDAC3 co-repressive complex modulates the leukemogenic potential of the transcription factor ERGNature CommunicationsSeptember 21, 2023Eitan Kugler, Shreyas Madiwale, Darren Yong, Julie A. I. Thoms, Yehudit Birger, David B. Sykes, Johannes Schmoellerl, Aneta Drakul, Valdemar Priebe, Muhammad Yassin, et al.108411, Biolegend PE anti-mouse CD34 Antibody Clone SA376A4, cat # 152203. Biolegend APC Annexin V, cat # 640919, Biolegend Western blot analysis In all, 2 × 106 cells were lysed using either RIPA buffer or CellLytic

(2) Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse modelNature communicationsJuly 4, 2023Hu M, Li T, Ma X, Liu S, Li C, Huang Z, Lin Y, Wu R, Wang S, Lu D, et al.evaluated with western blot. To stain for cell death markers, APC-conjugated Annexin V (Biolegend, 640919) was incubated at 4 °C for 15 min with the endothelial cells. PI was added right before acquisition

(3) NFATc1 induction by an intronic enhancer restricts NKT γδ cell formationiScienceMarch 17, 2023Sabrina Giampaolo, Cristina M. Chiarolla, Konrad Knöpper, Martin Vaeth, Matthias Klein, Azeem Muhammad, Tobias Bopp, Friederike Berberich-Siebelt, Amiya K. Patra, Edgar Serfling, et al.16561-29-8 Ionomycin,Thermo Fisher Scientific,Cat# I24222; CAS 56092-82-1 APC Annexin V,Biolegend,Cat# 640919 "Mouse CD1d PBS-57 tetramer, BV421",NIH Tetramer Core Facility,N/A mCD1d control,NIH Tetramer Core

(4) Phosphatidylserine-Exposing Annexin A1-Positive Extracellular Vesicles: Potential Cancer BiomarkersVaccinesMarch 13, 2023Perez GI, Bernard MP, Vocelle D, Zarea AA, Saleh NA, Gagea MA, Schneider D, Bauzon M, Hermiston T, Kanada M.bottom chamber slides (80807, Ibidi). Isolated EVs were incubated with 0.8 µg/mL APC-labeled annexin A5 (640919, BioLegend, San Diego, CA, USA) and 4.8 µg/mL FITC-GlaS for 30 min at room temperature and directly

(5) Insufficient epitope-specific T cell clones are responsible for impaired cellular immunity to inactivated SARS-CoV-2 vaccine in older adultsNature AgingMarch 13, 2023Chanchan Xiao, Zhiyao Ren, Bei Zhang, Lipeng Mao, Guodong Zhu, Lijuan Gao, Jun Su, Jiezhou Ye, Ze Long, Yue Zhu, et al.evaluated after 16 hours, and tetramer-specific CD8 T cells and apoptosis marker Annexin V-APC (BioLegend, 640919) on T2 cells were evaluated after 7 days. On day 7, cells were re-stimulated with peptides for 4 hours

(6) Enhancer Clusters Drive Type I Interferon-Induced TRAIL Overexpression in Cancer, and Its Intracellular Protein Accumulation Fails to Induce ApoptosisCancers (Basel)February 3, 2023Carolina Di Benedetto, Taimoor Khan, Santiago Serrano-Saenz, Anthony Rodriguez-Lemus, Chananat Klomsiri, Tim-Mathis Beutel, Alysia Thach, Henning Walczak, Paola Betancurcollected, washed with HBSS, and stained for 30 min with APC Annexin V (Biolegend; catalogue number 640919) in Annexin V binding buffer (Biolegend) at room temperature. After washing, Annexin V-positive events

(7) NSMCE2, a novel super-enhancer-regulated gene, is linked to poor prognosis and therapy resistance in breast cancerBMC CancerOctober 12, 2022Carolina Di Benedetto, Justin Oh, Zainab Choudhery, Weiquan Shi, Gilmer Valdes, Paola Betancurflow cytometry Cells were collected, washed with HBSS and stained with APC Annexin V (Biolegend, Catalogue number 640919) in Annexin V binding buffer (Biolegend) for 30 min at room temperature. After washing, Annexin V positive

(8) Developmental maturation of the hematopoietic system controlled by a Lin28b-let-7-Cbx2 axisCell ReportsApril 5, 2022Dahai Wang et al.AB_2251160 ,, "Chemicals, peptides, and recombinant proteins",, ,, APC-annexin V,BioLegend,Cat#640919 Dexamethasone,Sigma-Aldrich,Cat#D4902 Doxycycline,Sigma-Aldrich,Cat#D9891 Geneticin™ Selective Antibiotic,Thermo Fisher Scientific

(9) Dissecting the cellular landscape and transcriptome network in viral myocarditis by single-cell RNA sequencingiScienceMarch 18, 2022Ninaad Lasrado, Nicholas Borcherding, Rajkumar Arumugam, Timothy K. Starr, Jay ReddyREAGENT or RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Annexin V-APC,BioLegend,Cat# 640919 Propidium Iodide-PE,BioLegend,Cat# 421301 ,, Bacterial and virus strains,Bacterial and virus strains,Bacterial

(10) SARS-CoV-2 variant B.1.1.7 caused HLA-A2+ CD8+ T cell epitope mutations for impaired cellular immune responseiScienceMarch 18, 2022Chanchan Xiao, Lipeng Mao, Zhigang Wang, Lijuan Gao, Guodong Zhu, Jun Su, Xiongfei Chen, Jun Yuan, Yutian Hu, Zhinan Yin, et al.RRID:AB_314303 APC anti-human CD69 (clone FN50),BioLegend,Cat#310909;RRID:AB_314844 APC Annexin V,BioLegend,Cat#640919 PerCP anti-human IFN-γ (clone 4S.B3),BioLegend,Cat#502524;RRID:AB_2616613 FITC anti-human Granzyme

(11) Obesity-induced galectin-9 is a therapeutic target in B-cell acute lymphoblastic leukemiaNature communicationsMarch 3, 2022Lee M, Hamilton JAG, Talekar GR, Ross AJ, Michael L, Rupji M, Dwivedi B, Raikar SS, Boss J, Scharer CD, et al.BMS500FI-300, eBioscience). For GFP-expressing mB-ALL cells, Annexin-V-APC/PI staining was used (cat# 640919, Biolegend). Cell cycle analysis was performed using the Click-iT EdU AlexaFluor 488 Flow Cytometry

(12) CD8+ T-Cell Epitope Variations Suggest a Potential Antigen HLA-A2 Binding Deficiency for Spike Protein of SARS-CoV-2Frontiers in ImmunologyJanuary 18, 2022Congling Qiu et al.tetramer-specific CD8+ T-cell proportion were measured, along with apoptosis marker Annexin V-APC (BioLegend Cat#640919, San Diego, California, USA) on T2 cells. Parameters were evaluated after 16 h and 7 days, respectively.

(13) Genetic alterations of the SUMO isopeptidase SENP6 drive lymphomagenesis and genetic instability in diffuse large B-cell lymphomaNature communicationsJanuary 12, 2022Schick M, Zhang L, Maurer S, Maurer HC, Isaakaidis K, Schneider L, Patra U, Schunck K, Rohleder E, Hofstetter J, et al.#25-0452-82), IgM (PE, eB121-15F), 1:50, rat, eBioscience #12-5890-82), AnnexinV (APC, 1:25, BioLegend #640919) For ChiP: SUMO1 (15 µg, rabbit, Abcam ab32058), SUMO2/3 (15 µg, mouse, Abcam ab81371), MYC (15 µg,

(14) BRAFV600E induces reversible mitotic arrest in human melanocytes via microRNA-mediated suppression of AURKBeLifeDecember 14, 2021Andrew S McNeal, Rachel L Belote, Hanlin Zeng, Marcus Urquijo, Kendra Barker, Rodrigo Torres, Meghan Curtin, A Hunter Shain, Robert HI Andtbacka, Sheri Holmen, et al.Annexin V binding buffer (BioLegend, 422201) at a concentration of 1×106 cell/ml. 5 µl APC Annexin V antibody (BioLegend, 640919) and 10 µl PI were added. Cells were gently vortexed and incubated in the dark at room temperature for

(15) Everolimus and plicamycin specifically target chemoresistant colorectal cancer cells of the CMS4 subtypeCell Death & DiseaseOctober 21, 2021Jiayin Deng, Ai-Ling Tian, Hui Pan, Allan Sauvat, Marion Leduc, Peng Liu, Liwei Zhao, Shuai Zhang, Hui Chen, Valérie Taly, et al.were resuspended in 100 μL Annexin V Binding Buffer (422201, Biolegend) containing 0.2 μL Annexin V (640919, Biolegend) and 0.1 μL DAPI. Samples were then incubated in the dark for 15 min. After that, the plates

(16) Optimization of antigen-specific CD8+ T cell activation conditions for infectious diseases including COVID-19STAR ProtocolsSeptember 17, 2021Chanchan Xiao, Congling Qiu, Jieping Deng, Jiezhou Ye, Lijuan Gao, Jun Su, Oscar Junhong Luo, Pengcheng Wang, Guobing Chen7-AAD viability staining solution,BioLegend,Cat#420403 APC anti-human CD69 (clone FN50),BioLegend,Cat#310909;RRID:AB_314844 APC Annexin V,BioLegend,Cat#640919 PerCP anti-human IFN-γ (clone 4S.B3),BioLegend,Cat#502524;RRID:AB_2616613 FITC anti-human Granzyme

(17) Exosomes from primed MSCs can educate monocytes as a cellular therapy for hematopoietic acute radiation syndromeStem cell research & therapyAugust 18, 2021Forsberg MH, Kink JA, Thickens AS, Lewis BM, Childs CJ, Hematti P, Capitini CM.plate for 3 days. After 3 days, cells were harvested and stained with antibodies for Annexin V (Cat# 640919) and CD34 (clone 561, cat# 343615) from BioLegend® and Ghost Dye™ Red 780 viability dye from Tonbo Biosciences

(18) Drug Repurposing to Identify a Synergistic High-Order Drug Combination to Treat Sunitinib-Resistant Renal Cell CarcinomaCancers (Basel)August 6, 2021Magdalena Rausch, Adriano Rutz, Pierre-Marie Allard, Céline Delucinge-Vivier, Mylène Docquier, Olivier Dormond, Paul J. Dyson, Jean-Luc Wolfender, Patrycja Nowak-Sliwinska

(19) Mesenchymal stem cell therapy ameliorates metabolic dysfunction and restores fertility in a PCOS mouse model through interleukin-10Stem cell research & therapyJuly 7, 2021Chugh RM, Park HS, El Andaloussi A, Elsharoud A, Esfandyari S, Ulin M, Bakir L, Aboalsoud A, Ali M, Ashour D, et al.markers using antibodies against Ki67 antibody (BioLegend, Cat no. 350514), Annexin-V (BioLegend, Cat no. 640919), IL-1β (R&D Systems, Cat no. IC8406A), and TNF-α (BioLegend, Cat no. 502943). In brief, treated cell

(20) Promoter usage regulating the surface density of CAR molecules may modulate the kinetics of CAR-T cells in vivoMolecular therapy. Methods & clinical developmentJune 11, 2021Ho JY, Wang L, Liu Y, Ba M, Yang J, Zhang X, Chen D, Lu P, Li J.

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APC Annexin V from BioLegend

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APC Annexin V from BioLegend

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