Asp-N, Sequencing Grade V1621 from Promega

Product Specs
ItemAsp-N, Sequencing Grade
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV1621
Quantity2microg
TypeEnzyme

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Identification of tyrosine sulfation in the variable region of a bispecific antibody and its effect on stability and biological activitymAbsDecember 31, 2023Christopher B. Lietz, Ekaterina Deyanova, Younhee Cho, Jon Cordia, Sarah Franc, Sally Kabro, Steven Wang, David Mikolon, Douglas D. Bankstrypsin (Promega, Catalog # VA9000), chymotrypsin (Promega, Catalog # V1061) and AspN (Promega, Catalog # V1621) in protease to mAb-X molar ratios of 1:10, 1:20 and 1:50, respectively. Following overnight 37°C incubation

(2) Designer Adaptor Proteins for Functional Conversion of Peptides to Small-Molecule Ligands toward In-Cell Catalytic Protein ModificationACS Central ScienceNovember 22, 2023Akiko Fujimura, Hisashi Ishida, Tamiko Nozaki, Shuhei Terada, Yuto Azumaya, Tadashi Ishiguro, Yugo R. Kamimura, Tomoya Kujirai, Hitoshi Kurumizaka, Hidetoshi Kono, et al.ng/μL Trypsin Gold (Promega, V5280), 10 ng/μL Glu-C (Promega, V1651), and/or 2 ng/μL Asp-N (Promega, V1621) in 50 mM NH4HCO3 aq with 0.02 or 0.03% ProteaseMAX at 37 °C for 3 h to overnight. The digestion enzymes

(3) Identification of a Novel Acid Sphingomyelinase Activity Associated with Recombinant Human Acid CeramidaseBiomoleculesNovember 6, 2023Xingxuan He, Edward H. Schuchmanratio of 1:10 (protein/enzyme; w:w) and the other half was digested overnight with Asp-N (Promega, #V1621) at a ratio of 1:25 (protein/enzyme; w:w) at 37 °C. Separation of the peptides was accomplished using

(4) Chloroplasts evolved an additional layer of translational regulation based on non-AUG start codons for proteins with different turnover ratesScientific ReportsJanuary 17, 2023Leelavathi Sadhu, Krishan Kumar, Saravanan Kumar, Abhishek Dass, Ranjana Pathak, Amit Bhardwaj, Pankaj Pandey, Nguyen Van Cuu, Bhupendra S. Rawat, Vanga Siva Reddyfor 15 min, dried, and digested with 50 µl enzyme solution containing 15 ng/μl Asparagine-N (Cat no. V1621, Promega) or Glu-C (Cat no. V165A, Promega) or trypsin in 25 mM NH4HCO3 for 18 to 20 h at 37 °C. Following

(5) PKC isoforms activate LRRK1 kinase by phosphorylating conserved residues (Ser1064, Ser1074 and Thr1075) within the CORB GTPase domainBiochemical JournalSeptember 30, 2022Asad U. Malik, Athanasios Karapetsas, Raja S. Nirujogi, Deep Chatterjee, Toan K. Phung, Melanie Wightman, Robert Gourlay, Nicholas A Morrice, Sebastian Mathea, Stefan Knapp, et al.was purchased from New England Biolabs. Trypsin/Lys-C mix (#V5073), Chymotrypsin (#V1062) and Asp-N (#V1621) were purchased from Promega. Bryostatin-1 (#2383) was purchased from TOCRIS. Recombinant Rab7A protein

(6) TAILS Identifies Candidate Substrates and Biomarkers of ADAMTS7, a Therapeutic Protease Target in Coronary Artery DiseaseMolecular & cellular proteomics : MCPApril 1, 2022MacDonald BT, Keshishian H, Mundorff CC, Arduini A, Lai D, Bendinelli K, Popp NR, Bhandary B, Clauser KR, Specht H, et al.sample was digested with trypsin and the other half was digested with sequencing grade AspN (Promega, V1621) at 37 °C overnight and then mixed together after quenching the reactions the next day. Five percent

(7) Function-guided proximity mapping unveils electrophilic-metabolite sensing by proteins not present in their canonical localesProceedings of the National Academy of SciencesFebruary 1, 2022Yi Zhao, Pierre A. Miranda Herrera, Dalu Chang, Romain Hamelin, Marcus J. C. Long, Yimon AyeBio-Rad. Proteomics data are provided in a single Excel file (corresponding to Dataset S1). Asp-N (V1621) and Arg-C (V1881) for mass spectrometry experiments were from Promega. Information on cloning primers,

(8) Epigenetic glycosylation of SARS-CoV-2 impact viral infection through DC&L-SIGN receptorsiScienceDecember 17, 2021Guo L, Liang Y, Li H, Zheng H, Yang Z, Chen Y, Zhao X, Li J, Li B, Shi H, et al.88805 DL-dithiothreitol,Sigma,Cat# R0861 Iodoacetamide,Sigma,Cat# I10188 Trypsin,Promega,Cat# VA5111 Asp-N,Promega,Cat# V1621 Glu-C,Promega,Cat# V1651 Chymotrypsin,Promega,Cat# V1062 ,, Critical commercial assays,Critical

(9) Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular contextCell metabolismDecember 7, 2021Morgenstern M, Peikert CD, Lübbert P, Suppanz I, Klemm C, Alka O, Steiert C, Naumenko N, Schendzielorz A, Melchionda L, et al.Gel,Sigma-Aldrich,Cat.#A2220 Antimycin A,Sigma-Aldrich,Cat.#A8674 "AspN, Sequencing Grade",Promega,Cat.# V1621 BAMBANKER,Wako,Cat.#306-14684 Bis-Tris,Carl Roth,Cat. #9140.8 Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone

(10) Photoredox-Catalyzed Decarboxylative C-Terminal Differentiation for Bulk- and Single-Molecule ProteomicsACS Chemical BiologyNovember 19, 2021Le Zhang, Brendan M. Floyd, Maheshwerreddy Chilamari, James Mapes, Jagannath Swaminathan, Steven Bloom, Edward M. Marcotte, Eric V. AnslynThermo Scientific. LysArginase (Cat #EMS0008) was purchased from Millipore Sigma (WI, USA). AspN (Cat#V1621) was purchased from Promega Inc. (Cat #V1621). 2,2,2-trifluoroethanol (Cat #T63002) and tris(2-carboxyethyl)phosphine

(11) Mapping specificity, cleavage entropy, allosteric changes and substrates of blood proteases in a high-throughput screenNature communicationsMarch 16, 2021Uliana F, Vizovišek M, Acquasaliente L, Ciuffa R, Fossati A, Frommelt F, Goetze S, Wollscheid B, Gstaiger M, De Filippis V, et al.proteases used in this study were purchased from commercial vendors, Trypsin (V5111), Glu-C (V1651), Asp-N (V1621) and Chymotrypsin (V1091) from Promega (USA) and Lys-C (125-05061) from Wako (Japan). The blood cascade

(12) Protein Turnover in Epithelial Cells and Mucus along the Gastrointestinal Tract Is Coordinated by the Spatial Location and MicrobiotaCell ReportsJanuary 28, 2020Liisa Arike, Andrus Seiman, Sjoerd van der Post, Ana M. Rodriguez Piñeiro, Anna Ermund, André Schütte, Fredrik Bäckhed, Malin E.V. Johansson, Gunnar C. Hansson,Thermo Scientific,24115 DTT,Sigma-Aldrich,D9163 Iodoacetamide,Sigma-Aldrich,I6125 LysC,Wako,125-05061 AspN,Promega,V1621 ,, Deposited Data,Deposited Data,Deposited Data ,, Proteomics data raw data,This paper,ProteomeXchange:

(13) An integrated workflow for crosslinking mass spectrometryMolecular Systems BiologySeptember 20, 2019Marta L Mendes, Lutz Fischer, Zhuo A Chen, Marta Barbon, Francis J O'Reilly, Sven H Giese, Michael Bohlke‐Schneider, Adam Belsom, Therese Dau, Colin W Combe, et al.,Thermo Fisher,26173 "sulfosuccinimidyl 4,4′‐azipentanoate",Thermo Fisher,21580 Trypsin,Thermo Fisher,90057 AspN,Promega,V1621 Chymotrypsin,Promega,V1061 GluC,Promega,V1651 NuPAGE™ Novex 4–12% Bis‐Tris,Thermo Fisher,NP0335BOX Dithiothreitol,Merck,1114740005 Iodoacetamide,SIGMA,I1149 Ammonium

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Asp-N, Sequencing Grade

Asp-N, Sequencing Grade from Promega

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Promega

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Asp-N, Sequencing Grade from Promega

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