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Cell Biology
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Proteinase K (20 mg/ml)
Proteinase K (20 mg/ml) from Cell Signaling Technology
Be the first to write a review!
Citations:
(11)
Supplier Page
Supplier Page from
Cell Signaling Technology for
Proteinase K (20 mg/ml)
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Proteinase K (20 mg/ml)
Company
Cell Signaling Technology
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
10012S
Applications
ChIP, C&R
Quantity
100 µl
Research Area
Related Products
Molecule Name
Proteinase K
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Cell Signaling Technology
3 Trask Lane
Danvers
,
Massachusetts
01923
United States
Phone
: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488
Company Profile
Website:
http://www.cellsignal.com/
Website:
Technical Support
Citations (11)
(1)
Histone lactylation driven by mROS-mediated glycolytic shift promotes hypoxic pulmonary hypertension
Journal of molecular cell biology
April 20, 2023
Chen J, Zhang M, Liu Y, Zhao S, Wang Y, Wang M, Niu W, Jin F, Li Z.
from beads. The cross-linking of ChIP-DNA and input sample was reversed by proteinase K
incubation
(
10012
,
Cell Signaling Technology
) at 65°C for 2 h and purified with
purification
columns. The ChIP-DNA was
(2)
RAD18 opposes transcription-associated genome instability through FANCD2 recruitment
PLoS genetics
December 1, 2022
Wells JP, Chang EY, Dinatto L, White J, Ryall S, Stirling PC.
future use. For DRIP, DNA samples were treated with RNAse A (
CST
#
7013
) followed by proteinase K (
CST
#
10012
) overnight at 65°C. DNA samples were purified with
Cell Signaling
spin columns (#
14209S
). DNA concentration
(3)
Integrative analysis and prediction of human R-loop binding proteins
G3 Genes|Genomes|Genetics
July 29, 2022
Arun Kumar, Louis-Alexandre Fournier, Peter C Stirling
further use. DNA samples for DRIP were treated with RNAse A (
CST
#
7013
) followed by proteinase K (
CST
#
10012
) overnight at 65°C. DNA samples were purified with
Cell Signaling
spin columns (#
14209S
). DNA concentration
(4)
Loss of NFE2L3 protects against inflammation-induced colorectal cancer through modulation of the tumor microenvironment
Oncogene
March 10, 2022
James Saliba et al.
Eluates were reverse cross‐linked by adding 6 μL 5 mol/L NaCl and 2 μL Proteinase K (
Cell Signaling
,
10012
), and incubated for 2 h at 65 °C. DNA was extracted and purified using Spin Columns (Cell Signaling,
(5)
Functional antagonism of chromatin modulators regulates epithelial-mesenchymal transition
Science advances
February 1, 2021
Serresi M, Kertalli S, Li L, Schmitt MJ, Dramaretska Y, Wierikx J, Hulsman D, Gargiulo G.
cross-linking was performed by adding 6 μl of 5 M NaCl and 2 μl of proteinase K (
Cell Signaling Technology
, #
10012
) and
incubation
for 2 hours at 65°C. The DNA was purified by means of spin columns and eluted in 50
(6)
α-Actinin 4 Links Vasopressin Short-Term and Long-Term Regulation of Aquaporin-2 in Kidney Collecting Duct Cells
Frontiers in physiology
January 1, 2021
Ho CH, Yang HH, Su SH, Yeh AH, Yu MJ.
then transferred to a new tube to reverse the crosslink with 200 mM NaCl and 2 μl proteinase K (Cat.
10012
,
Cell Signaling Technologies
, United States) at 65°C for 2 h. The
chromatin
DNA was then purified with
(7)
CD1d1 intrinsic signaling in macrophages controls NLRP3 inflammasome expression during inflammation
Science advances
October 1, 2020
Cui S, Wang C, Bai W, Li J, Pan Y, Huang X, Yang H, Feng Z, Xiang Q, Fei L,
et al
.
eluted from antibody/
protein G magnetic beads
and reversed cross-links with protein K (2 μl per sample; #
10012
,
CST
). DNA was extracted and purified using spin columns (#10010, CST).
qPCR
was performed with special
(8)
Inhibition of Microglial TGFβ Signaling Increases Expression of
Mrc1
Frontiers in cellular neuroscience
January 1, 2020
von Ehr A, Attaai A, Neidert N, Potru PS, Ruß T, Zöller T, Spittau B.
elution of
chromatin
was achieved. Cross-linking was reversed using 2 μl Proteinase K (
Cell Signaling
, #
10012
) for 2 h at 65°C. Finally,
DNA purification
in spin columns was performed, and promoter fragments were
(9)
Topologically Associated Domains Delineate Susceptibility to Somatic Hypermutation
Cell reports
December 17, 2019
Senigl F, Maman Y, Dinesh RK, Alinikula J, Seth RB, Pecnova L, Omer AD, Rao SSP, Weisz D, Buerstedde JM,
et al
.
pelleted and the supernatant was moved to a new 1.5 mL Eppendorf tube and 2 μl of Proteinase K (
CST
#
10012
) added. The mixture was incubated for 2 hr at 65°C and then DNA was purified using SimpleChIP
DNA Purification
(10)
Regulation of CXCL1 chemokine and CSF3 cytokine levels in myometrial cells by the MAFF transcription factor
Journal of cellular and molecular medicine
April 1, 2019
Saliba J, Coutaud B, Solovieva V, Lu F, Blank V.
Eluates were reverse cross‐linked by adding 6 μL 5 mol/L NaCl and 2 μL Proteinase K (
Cell Signaling
,
10012
), and incubated for 2 hours at 65°C. DNA was extracted and purified using Spin Columns (Cell Signaling,
(11)
Microglia-Specific Expression of Olfml3 Is Directly Regulated by Transforming Growth Factor β1-Induced Smad2 Signaling
Frontiers in Immunology
July 26, 2018
Nicolas Neidert, Alexander von Ehr, Tanja Zöller, Björn Spittau
chromatin
was performed. The protein cross-link was reversed by using 2 µl Proteinase K (
Cell Signaling
, #
10012
) for 2 h at 65°C. Finally,
DNA purification
in spin columns was performed and the amount of DNA was
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Proteinase K (20 mg/ml) from Cell Signaling Technology
Supplier Page
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Pricing Info
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Company Info
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