Fig 1: Example of kinetic data and fitting to the Michaelis-Menten equationKinetic evaluation of (A) substrate ETDKmyr (6e) for HDAC11 (Moreno-Yruela et al., 2018) and (B) substrate LGKac (1a) for HDAC3. HDAC11 experiments were linear for up to 65 min, whereas HDAC3 only retained linearity for 25 min (progression data represent mean ± SD of 2 internal replicates). XY data sheets are created with substrate concentrations and corresponding slopes from at least 2 experiments, and data are fitted to the Michaelis-Menten equation to generate the represented curves (steady-state data represent mean ± SEM of 2 individual experiments). 1.5-fold dilutions of substrate concentration are preferred, as in B, for better definition of the curve and calculation of KM and kcat parameters. HDAC11 plot reprinted with permission from Moreno-Yruela et al. (2018).