Fig 1: pDNA and pRNA capping by ApeTpt1 using biotin-NAD+ as substrate. Reaction mixtures (10 µl) containing 100 mM Tris–HCl (pH 7.5), 0.2 µM (2 pmol) 5' 32P-labeled 10-mer pDNA or pRNA substrates (shown at bottom), 50 µM NAD+ or biotin-NAD+ (where specified by +), and 0.5 µM (5 pmol) ApeTpt1 (where indicated by +) were incubated at 37°C for 60 min. The products were analyzed by urea-PAGE and visualized by autoradiography. 6-Biotin-17-NAD+ was from BPS Bioscience (catalog # 80610).
Supplier Page from BPS Bioscience, Inc. for NAD+, Biotin-Labeled