M-MLV Reverse Transcriptase M1302 from MilliporeSigma

Product Specs
ItemM-MLV Reverse Transcriptase
CompanyMilliporeSigma
Price Supplier Page View Company Product Page
Catalog NumberM1302
ApplicationsVirus Characterization: RT-PCR Analysis

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MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

Company Profile

Website: www.SigmaAldrich.com/

(1) Anti-TLR4 biological response to titanium nitride-coated dental implants: anti-inflammatory response and extracellular matrix synthesisFront Bioeng BiotechnolDecember 5, 2023Stefano Oliva, Francesca Diomede, Ylenia Della Rocca, Antonella Mazzone, Guya Diletta Marconi, Jacopo Pizzicannella, Oriana Trubiani, Giovanna MurmuraCat# 12183018A). According to the technical bulletin, M-MLV Reverse Transcriptase (Sigma-Aldrich, Cat# M1302) was used to reverse-transcribe the total RNA into cDNA for 10 min at 70°C, 50 min at 37°C, and 10 min

(2) Functionally distinct regions of the locus Leishmania major response 15 control IgE or IFNγ level in addition to skin lesionsFront ImmunolAugust 3, 2023Imtissal Krayem, Yahya Sohrabi, Helena Havelková, Elena S. Gusareva, Hynek Strnad, Marie Čepičková, Valeryia Volkova, Iryna Kurey, Jarmila Vojtíšková, Milena Svobodová, et al.States, M6101) and then reverse-transcribed using 100 units of M-MLV Reverse Transcriptase (Sigma, M1302) with 1×MLV reverse transcriptase buffer, 1.4 µM of random hexamers (Thermo Fisher, Massachusetts, United

(3) Inhibition of LPS-Induced Inflammatory Response of Oral Mesenchymal Stem Cells in the Presence of Galectin-3BiomedicinesMay 24, 2023Alessia Paganelli, Francesca Diomede, Guya Diletta Marconi, Jacopo Pizzicannella, Thangavelu Soundara Rajan, Oriana Trubiani, Roberto PaganelliRNA quantification, 1 µg of total RNA of each sample was retrotranscribed through M-MLV Reverse Transcriptase (M1302 Sigma-Aldrich). The cDNA samples obtained were used for the Real-Time PCR, executed through the Mastercycler

(4) The impact of metabolic endotoxaemia on the browning process in human adipocytesBMC MedicineApril 19, 2023Farah Omran, Alice M. Murphy, Awais Z. Younis, Ioannis Kyrou, Jana Vrbikova, Vojtech Hainer, Petra Sramkova, Martin Fried, Graham Ball, Gyanendra Tripathi, et al.Sigma-Aldrich, UK) and cDNA synthesis was undertaken using a Sigma-Aldrich mRNA reverse transcription kit (#M1302-40KU, UK) according to the manufacturer’s instructions. Primers for mRNA were synthesized by Sigma Aldrich

(5) Enriched Graphene Oxide-Polypropylene Suture Threads Buttons Modulate the Inflammatory Pathway Induced by Escherichia coli LipopolysaccharideInternational Journal of Molecular SciencesApril 1, 2023Luigia Fonticoli, Francesca Diomede, Antonio Nanci, Antonella Fontana, Ylenia Della Rocca, Dainelys Guadarrama Bello, Serena Pilato, Oriana Trubiani, Jacopo Pizzicannella, Guya Diletta Marconifor each sample. One microgram of total RNA was retrotranscribed using M-MLV Reverse Transcriptase (M1302 Sigma-Aldrich) to synthesize cDNA for 10 min at 70 °C, 50 min at 37 °C and 10 min at 90 °C according

(6) Long noncoding RNA ACART knockdown decreases 3T3-L1 preadipocyte proliferation and differentiationOpen Life SciencesFebruary 9, 2023Renyan Huang, Chenyan Shi, Guobin Liutranscription. cDNA was synthesized using Moloney’s murine leukemia virus reverse transcriptase (Product number: M1302; Sigma-Aldrich) and random primers (3 µg/µL; Product number: 48190011; Invitrogen, CA, USA). qRT-PCR

(7) Forced expression of the non-coding RNA miR-17âˆ¼92 restores activation and function in CD28-deficient CD4+ T cellsiScienceNovember 18, 2022Marianne DÃ¶lz, Marko Hasiuk, John D. Gagnon, Mara Kornete, Romina Marone, Glenn Bantug, Robin Kageyama, Christoph Hess, K. Mark Ansel, Denis Seyres, et al.TRI Reagent,Sigma-Aldrich,T9424-200ML 1-Bromo-3-chloropropane,Sigma-Aldrich,B9673-200ML M-MLV Reverse Transcriptase,Sigma-Aldrich,M1302-40KU TaqMan™ MicroRNA Reverse Transcription Kit,ThermoFisher,4366597 "TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

(8) Consequences of PDGFR a + fibroblast reduction in adult murine heartseLifeSeptember 23, 2022Jill T Kuwabara, Akitoshi Hara, Sumit Bhutada, Greg S Gojanovich, Jasmine Chen, Kanani Hokutan, Vikram Shettigar, Anson Y Lee, Lydia P DeAngelo, Jack R Heckl, et al.2000 instrument (Thermo Fisher). The cDNA was synthesized using M-MLV Reverse Transcriptase (Sigma, M1302) and random hexamer primer (Thermo Fisher, SO142). The qPCR analysis was performed using PowerUp SYBR Green Master Mix

(9) ESCRT-I fuels lysosomal degradation to restrict TFEB/TFE3 signaling via the Rag-mTORC1 pathwayLife Science AllianceJuly 1, 2022Marta Wróbel et al.nonamers (R7647; Sigma-Aldrich), oligo(dT)23 (O4387; Sigma-Aldrich), and M-MLV reverse transcriptase (M1302; Sigma-Aldrich) were used. Primers were designed using the NCBI Primer designing tool and custom-synthesized

(10) Phosphorylation-dependent association of human chromatin protein PC4 to linker histone H1 regulates genome organization and transcriptionNucleic Acids ResearchJune 24, 2022Pallabi Mustafi, Mingli Hu, Sujata Kumari, Chandrima Das, Guohong Li, Tapas K Kundufrom ∼2 × 104 cells. 0.5μg of RNA was reverse transcribed using MMLV reverse transcriptase (Sigma, M1302) using oligo‐dT primers. Resulting cDNAs were quantified by RT‐PCR using KAPA SYBR FAST Universal qPCR master mix

(11) MiR-378a inhibits glucose metabolism by suppressing GLUT1 in prostate cancerOncogeneMarch 4, 2022A. Cannistraci, P. Hascoet, A. Ali, P. Mundra, N. W. Clarke, V. Pavet, R. Maraisof total RNA was reverse transcribed into cDNA using M-MLV Reverse Transcriptase (Sigma Aldrich, cat#M1302) and stored at −20 °C. Q-PCR analysis was performed using FastStart Universal Probe Master (ROX)(Sigma

(12) RNA-Seq is not required to determine stable reference genes for qPCR normalizationPLOS Computational BiologyFebruary 28, 2022Nirmal Kumar Sampathkumar et al.RNA was reverse transcribed with Random Primers (Promega C1181) and MMLV Reverse Transcriptase (Sigma M1302) according to prescribed protocols. qPCR was performed using Absolute SYBR ROX 2X qPCR mix (Thermo AB1162B)

(13) Genetic Influence on Frequencies of Myeloid-Derived Cell Subpopulations in MouseFrontiers in ImmunologyJanuary 26, 2022Imtissal Krayem et al.(Promega, M6101) and then reverse transcribed using 100 units of M-MLV Reverse Transcriptase (Sigma, M1302) with 1xMLV reverse transcriptase buffer, 1.4 µM of random hexamers (Thermo Fisher, N8080127), 2.5 units

(14) Myogenin controls via AKAP6 non-centrosomal microtubule-organizing center formation at the nuclear envelopeeLifeOctober 4, 2021Robert Becker, Silvia Vergarajauregui, Florian Billing, Maria Sharkova, Eleonora Lippolis, Kamel Mamchaoui, Fulvia Ferrazzi, Felix B Engelreverse transcribed using Oligo (dT) 12-18mer primers and M-MLV Reverse Transcriptase (Sigma-Aldrich #M1302) according to the manufacturer’s instructions. For PCR, ~20 ng of cDNA were used with Redtaq master

(15) The NMD Pathway Regulates GABARAPL1 mRNA during the EMTBiomedicinesSeptember 23, 2021Timothée Baudu et al.analysis, 1 µg of total RNAs were reverse-transcribed using 60 units of M-MLV Reverse Transcriptase (Sigma, M1302), 1.25 µM of oligo (dT)23 (Eurogentec) and 1.25 µM of random primers (Promega, C1181, Madison, WI, USA).

(16) Vascepa protects against high-fat diet-induced glucose intolerance, insulin resistance, and impaired Î²-cell functioniScienceAugust 20, 2021Dana Al Rijjal, Ying Liu, Mi Lai, Youchen Song, Zahra Danaei, Anne Wu, Haneesha Mohan, Li Wei, Francisco J. Schopfer, Feihan F. Dai, et al.10,297-018, Invitrogen), Oligo dT (Cat# 18418012, Invitrogen) and M-MLV reverse transcriptase (Cat# M1302-40KU, Sigma Aldrich) as per the manufacturer's instructions. qPCR was performed using SYBR green (Cat#

(17) SOX21 modulates SOX2-initiated differentiation of epithelial cells in the extrapulmonary airwayseLifeJuly 21, 2021Evelien Eenjes, Marjon Buscop-van Kempen, Anne Boerema-de Munck, Gabriela G Edel, Floor Benthem, Lisette de Kreij-de Bruin, Marco Schnater, Dick Tibboel, Jennifer Collins, Robbert J Rottier(ThermoFisher Scientific). First strand cDNA was synthesized using 2 µg RNA, MLV Reverse transcriptase (Sigma, M1302), and Oligo-dT primers (self-designed: 23xT+A, 23xT+C, and 23xT+G). For one qRT–PCR, 0.5 µl of cDNA

(18) Retracing Schwann Cell Developmental Transitions in Embryonic Dissociated DRG/Schwann Cell Cocultures in MiceFrontiers in Cellular NeuroscienceMay 20, 2021Venkat Krishnan Sundaram, Tatiana El Jalkh, Rasha Barakat, Camille Julie Isabelle Fernandez, Charbel Massaad, Julien GrenierRNA was reverse transcribed with Random Primers (Promega C1181) and MMLV Reverse Transcriptase (Sigma M1302) according to prescribed protocols. Quantitative Real-time PCR (qPCR) was performed using Absolute SYBR

(19) EBV miRNAs are potent effectors of tumor cell transcriptome remodeling in promoting immune escapePLOS PathogensMay 6, 2021Nathan Ungerleider, Whitney Bullard, Mehmet Kara, Xia Wang, Claire Roberts, Rolf Renne, Scott Tibbetts, Erik K. Flemingtonapproach[49]. RNA was reverse transcribed using M-MLV reverse transcriptase (Millipore Sigma, catalog no. M1302) according to the manufacturer’s protocol, using pooled microRNA-specific RT primers at a final concentration

(20) Reverse Transcriptase: From Transcriptomics to Genome EditingTrends in BiotechnologyFebruary 1, 2021Samara Martín-Alonso, Estrella Frutos-Beltrán, Luis Menéndez-Arias(https://www.promega.es/products/pcr/rt‐pcr/m‐mlv‐reverse‐ transcriptase/?catNum=M1701) (4) Sigma‐Merck (https://www.sigmaaldrich.com/catalog/product/sigma/m1302?lang=es®ion=ES&gclid=EAIaIQo bChMIpKiCjamu6QIVFJzVCh0C5Ag‐EAAYASAAEgLSwfD_BwE) (5) Bagnoli, J.W.

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M-MLV Reverse Transcriptase from MilliporeSigma

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M-MLV Reverse Transcriptase from MilliporeSigma

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