Tumor Necrosis Factor-α human H8916 from MilliporeSigma

Product Specs
ItemTumor Necrosis Factor-α human
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberH8916
Molecular Weight17 kDa (glycosylated) ~17.4 kDa
Specieshuman
Molecule NameADRA1D, ADRA1A, ADRA1B
NCBI Full Gene Nameadrenoceptor alpha 1A, adrenoceptor alpha 1B, adrenoceptor alpha 1D
NCBI Gene AliasesADRA1A, ADRA1C, ALPHA1, ADRA1, ALPHA1BAR, ADRA1R, DAR, ADRA1L1, dJ779E11.2, ALPHA1AAR
Applicationsmammalian cell culture
Quantity10µG

Add to Compare List

MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

Company Profile

Website: www.SigmaAldrich.com/

(1) Differential cardiomyocyte transcriptomic remodeling during in vitro Trypanosoma cruzi infection using laboratory strains provides implications on pathogenic host responsesTrop Med HealthDecember 7, 2023Katherine-Sofia Candray-Medina, Yu Nakagama, Masamichi Ito, Shun Nakagama, Evariste Tshibangu-Kabamba, Norihiko Takeda, Yuki Sugiura, Yuko Nitahara, Yu Michimuko-Nagahara, Natsuko Kaku, et al.was collected to infect HL-1 cardiomyocytes at an MOI of 10:1. Mouse TNF-α (100 ng/mL; Sigma-Aldrich H8916, Missouri, USA) was added to the culture medium of the positive control wells [19, 21, 26–31]. CCdT

(2) Rheumatoid Synovial Fluid and Acidic Extracellular pH Modulate the Immunomodulatory Activity of Urine-Derived Stem CellsInternational Journal of Molecular SciencesNovember 1, 2023Michaela Cehakova, Dana Ivanisova, Magdalena Strecanska, Jana Plava, Zuzana Varchulova Novakova, Andreas Nicodemou, Stefan Harsanyi, Martina Culenova, Sona Bernatova, Lubos Danisovicin SFM hg DMEM (Sigma-Aldrich, St. Louis, MO, USA) with RASFs (1 mg/mL) and recombinant human TNF-α (H8916, Sigma-Aldrich, St. Louis, MO, USA; 10 ng/mL) for 48 h. 4.6. ATSCs Treatment Protocol To evaluate

(3) Inhibition of TGF-β signaling, invasion, and growth of cutaneous squamous cell carcinoma by PLX8394OncogeneOctober 20, 2023Elina Siljamäki, Pilvi Riihilä, Ujjwal Suwal, Liisa Nissinen, Pekka Rappu, Markku Kallajoki, Veli-Matti Kähäri, Jyrki Heinoor as a control with 0.1% DMSO o/n at 37 °C. Next day, the cells were treated with TNF-α (10 ng/ml; H8916, Sigma-Aldrich) and IL-1β (10 ng/ml; 407615-5, VWR) for 30 min/+37 °C, or with D-sorbitol (400 mM, Sigma

(4) Effect of P53 nuclear localization mediated by G3BP1 on ferroptosis in acute liver failureApoptosis : an international journal on programmed cell deathAugust 1, 2023Li W, Li W, Li X, Wang L, Wang Y.No. 12,483,020) were purchased from Gibco (Grand Island, USA). LPS (Cat. No. L4391), TNF-α (Cat. No. H8916) and D-galactosamine (D-Gal) (Cat. No. G0500) were purchased from Sigma (Grand Island, USA). A bicinchoninic

(5) Robust induction of functional astrocytes using NGN2 expression in human pluripotent stem cellsiScienceJuly 21, 2023Martin H. Berryer, Matthew Tegtmeyer, Loïc Binan, Vera Valakh, Anna Nathanson, Darina Trendafilova, Ethan Crouse, Jenny A. Klein, Daniel Meyer, Olli Pietiläinen, et al.96-well plates and incubated 24 hours later with human Tumor Necrosis Factor alpha (Sigma Aldrich, H8916, 100ng.mL-1) or 0.1% Bovine Serum Albumin (Sigma Aldrich, A0281-10G) in Astrocyte medium (ScienCell,

(6) Probing expression of E-selectin using CRISPR-Cas9-mediated tagging with HiBiT in human endothelial cellsiScienceJuly 21, 2023Lydia Ogrodzinski, Simon Platt, Joelle Goulding, Cameron Alexander, Tracy D. Farr, Jeanette Woolard, Stephen J. Hill, Laura E. KilpatrickLB Broth (Lennox),Sigma Aldrich (UK),Cat# L3022 Tumor Necrosis Factor-α human,Sigma Aldrich (UK),Cat# H8916 Recombinant Human VEGF 165 Protein (with carrier),R&D systems (USA),Cat# 293-VE-050 Histamine dihydrochloride,Sigma Aldrich

(7) The Impact of Hydroxychloroquine on Primary Feto-Placental Endothelial Cells from Healthy and Early-Onset Preeclamptic PlacentasInternational Journal of Molecular SciencesJune 30, 2023Maja Gajić, Bianca Schröder-Heurich, Monika Horvat Mercnik, Mila Cervar-Zivkovic, Christian Wadsack, Frauke von Versen-Höynck, Karoline Mayer-Pickelsterile water (Fresenius Kabi, Bad Homburg, Germany) to the concentration of 10 mg/mL. Cytokines: TNF-α (H8916, Merck, Darmstadt, Germany), IL-6 (I1395, Merck, Darmstadt, Germany) and recombinant human IL-1β (200-Q1B,

(8) TMEM123 a key player in immune surveillance of colorectal cancerFrontiers in ImmunologyJune 23, 2023Elisa Pesce, Chiara Cordiglieri, Mauro Bombaci, Serenella Eppenberger-Castori, Stefania Oliveto, Cristina Manara, Mariacristina Crosti, Caner Ercan, Mairene Coto, Andrea Gobbini, et al.P0409 Rapamycin,Sigma-Merk,Cat# 553210 RIPA buffer,Sigma-Merk,Cat# R0278 tumor necrosis factor alpha,Sigma-Merk,Cat# H8916 WheatGermAgglutinine- OregonGreen 488,Invitrogen Molecular Probes - Thermo Fisher,Cat# W6748 Critical

(9) Postmortem Brains from Subjects with Diabetes Mellitus Display Reduced GLUT4 Expression and Soma Area in Hippocampal Neurons: Potential Involvement of InflammationCellsApril 25, 2023Caio Yogi Yonamine, Marisa Passarelli, Claudia Kimie Suemoto, Carlos Augusto Pasqualucci, Wilson Jacob-Filho, Venâncio Avancini Ferreira Alves, Suely Kazue Nagahashi Marie, Maria Lucia Correa-Giannella, Luiz Roberto Britto, Ubiratan Fabres Machadosubjected to four different experimental design protocols: (1) tumor necrosis factor (TNF) (Sigma-Aldrich, #H8916) at 20 ng/mL for 3 h; (2) glycated albumin (GA) or non-glycated albumin at 0.4 mg/mL for 24 h; (3) RGFP-996

(10) Expression of GnT-III decreases chemoresistance via negatively regulating P-glycoprotein expression: Involvement of the TNFR2-NF-κB signaling pathwayJournal of Biological ChemistryApril 1, 2023Wanli Song, Caixia Liang, Yuhan Sun, Sayaka Morii, Shin Yomogida, Tomoya Isaji, Tomohiko Fukuda, Qinglei Hang, Akiyoshi Hara, Miyako Nakano, et al.from Vector Laboratories; E4-PHA agarose (J311), which is from J-OIL MILLS; Recombinant human TNFα (H8916), BAY 11-7082 (B5556), dasatinib (SML2589), and doxycycline hyclate (D9891), which were acquired from

(11) A Monoclonal Human Alveolar Epithelial Cell Line (“Arlo”) with Pronounced Barrier Function for Studying Drug Permeability and Viral InfectionsAdvanced ScienceMarch 1, 2023Patrick Carius, Annemarie Jungmann, Marco Bechtel, Alexander Grißmer, Annette Boese, Gilles Gasparoni, Abdulrahman Salhab, Ralf Seipelt, Klaus Urbschat, Clémentine Richter, et al.stimulation medium supplemented with tumor necrosis factor alpha (TNF α) (human 25 ng mL−1; Sigma‐Aldrich, H8916‐10UG) and interferon gamma (INF γ) (human 30 ng mL−1; Sigma‐Aldrich, I17001‐100UG). The stimulation

(12) New Insights into Bitopic Orthosteric/Allosteric Ligands of Cannabinoid Receptor Type 2International Journal of Molecular SciencesJanuary 21, 2023Rebecca Ferrisi, Beatrice Polini, Caterina Ricardi, Francesca Gado, Kawthar A. Mohamed, Giovanna Baron, Salvatore Faiella, Giulio Poli, Simona Rapposelli, Giuseppe Saccomanni, et al.penicillin (100 U/mL) (Sigma-Aldrich, Milan, Italy). LPS (Cat. number L4391) and TNFα (Cat. number H8916) were purchased from Sigma-Aldrich (Milan, Italy). 3.2. HitHunter cAMP Assay This method was conducted

(13) Inhibition of NF-kB/IL-6/JAK2/STAT3 Pathway and Epithelial-Mesenchymal Transition in Breast Cancer Cells by AzilsartanMoleculesNovember 13, 2022Rania Alaaeldin, Fares E. M. Ali, Amany Abdlrehim Bekhit, Qing-Li Zhao, Moustafa Fathytargeting and inhibiting the activated NF-kB pathway in MCF-7 and MDA-MB-231 breast cancer cells, TNFα (#H8916, Sigma Aldrich, St Louis, MO, USA) was used to induce NF-kB in both MCF-7 and MDA-MB-231 cancer cells,

(14) The potential pathogenic roles of S100A8/A9 and S100A12 in patients with MPO-ANCA-positive vasculitisBMC ImmunologySeptember 10, 2022Xue Bai, Peng-Cheng Xu, Tong Chen, Hao-Miao Zhang, Si-Jing Wu, Xia Yang, Shan Gao, Jun-Ya Jia, Jian-Qing Jiang, Tie-Kun YanS100A12 by neutrophils stimulated with ANCA Neutrophils (1 × 106/ml) were primed with 2 ng/ml TNF-α (H8916, Sigma-Aldrich) at 37 °C for 15 min and cultured with normal control IgG from healthy donors and different

(15) Regulation of Adipose Progenitor Cell Expansion in a Novel Micro-Physiological Model of Human Adipose Tissue Mimicking Fibrotic and Pro-Inflammatory MicroenvironmentsCellsSeptember 7, 2022Dani V, Bruni-Favier S, Chignon-Sicard B, Loubat A, Doglio A, Dani C.Germany) supplemented with 2% FCS, 22.5 μg/mL ascorbic acid, 1 μg/mL heparin and 10 ng/mL TNFα (Cat: H8916, Sigma-Aldrich, St. Louis, MO, USA) or 5 ng/mL TGFβ1 (Cat: 100-21, Preprotech, Neuilly-sur-Seine, France). 2.5.

(16) Culture-Associated DNA Methylation Changes Impact on Cellular Function of Human Intestinal OrganoidsCellular and Molecular Gastroenterology and HepatologyAugust 28, 2022Rachel D. Edgar, Francesca Perrone, April R. Foster, Felicity Payne, Sophia Lewis, Komal M. Nayak, Judith Kraiczy, Aurélie Cenier, Franco Torrente, Camilla Salvestrini, et al.splitting in the growth medium, followed by 24 hours of treatment with recombinant human protein TNFα (H8916; Sigma Aldrich, Burlington, MA) at 40 ng/mL or IFNγ (PHC4031; Life Technologies, Carlsbad, CA) at 20

(17) Quercetin, a flavonoid, combats rotavirus infection by deactivating rotavirus-induced pro-survival NF-κB pathwayFrontiers in MicrobiologyAugust 2, 2022Shreya Banerjee, Rakesh Sarkar, Arpita Mukherjee, Shin-ichi Miyoshi, Kei Kitahara, Prolay Halder, Hemanta Koley, Mamta Chawla-SarkarUSA. Protease inhibitor cocktail (P2714), phosphatase inhibitor cocktail 2 (P5726), MTT (M5655), TNFα (H8916), and Bay11-7082 (B5556) were procured from Merck (Sigma-Aldrich, USA). Rabbit polyclonal antibody against

(18) Pro-inflammatory cytokines mediate the epithelial-to-mesenchymal-like transition of pediatric posterior fossa ependymomaNature communicationsJuly 8, 2022Aubin RG, Troisi EC, Montelongo J, Alghalith AN, Nasrallah MP, Santi M, Camara PG.treated with 4 ng/ml TGF-β1 (Sigma-Aldrich, cat. #H8541-5UG), 10 ng/ml TNF-α (Sigma-Aldrich, cat. # H8916-10UG), or both, for 5 days before RNA extraction. Media was replenished every 48 h. Cells were plated

(19) Characterization and Validation of In Vitro and In Vivo Models to Investigate TNF-Î±-Induced Inflammation in Retinal DiseasesTranslational Vision Science & TechnologyMay 17, 2022Carina M. Weigelt, Nina Zippel, Holger Fuchs, Anna-Kaisa RimpelÃ¤, Tanja SchÃ¶nberger, Birgit Stierstorfer, Remko A. Bakker, Norbert H. RedemannTNF-α–induced expression analysis assays, cells were stimulated with 10 ng/mL recombinant human TNF-α (H8916, Sigma Aldrich Corp.) for 24 hours with or without 58.8 nM golimumab (Simponi, MSD Sharp & Dohme). THP-1

(20) Metformin Treatment Regulates the Expression of Molecules Involved in Adiponectin and Insulin Signaling Pathways in Endometria from Women with Obesity-Associated Insulin Resistance and PCOSInternational Journal of Molecular SciencesApril 1, 2022Maria Lorena Oróstica, Isis Astorga, Francisca Plaza-Parrochia, Cristian Poblete, Rodrigo Carvajal, Víctor García, Carmen Romero, Margarita Vega(Merck), Darmstadt, Germany); a human recombinant protein called TNFα (tumor necrosis factor alpha, #H8916, Sigma-Aldrich, MO, USA) and metformin (1,1-Dimethylbiguanide hydrochloride, #1115-70.4, Sigma-Aldrich

Be the First to Write a Review!

Tumor Necrosis Factor-α human from MilliporeSigma

MilliporeSigma

Citations (60)

Reviews

Tumor Necrosis Factor-α human from MilliporeSigma

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map