Fig 1: Structure analysis of epitopes.3D structures of protein targets with highlighted linear epitopes in green where the corresponding antibody fraction showed bands of correct molecular weight in Western blot analysis and in red where the antibody fraction did not bind the target protein. SYNJ2BP (2eno.pdb), TYMP (2jof.pdb), WARS (1r6t.pdb), CD4 (1wio.pdb), OTC (1oth.pdb), CRABP2 (2g7b.pdb), PDXP (2cft.pdb) and EGFR (3njp.pdb).
Fig 2: Western blot performance of antibody fractions.(A) The ability of the different antibody fractions to detect a protein of the expected molecular weight in Western blot is shown in green (positive) or red (negative). The number indicates the relative amount in percent of each antibody fraction and a total of antibody fractions towards linear epitopes that are able to bind the target protein in Western blot. (B) Western blots analysis of eight different protein targets. Detection of protein targets using polyclonal antibody, antibody fraction towards conformational epitopes and one representative antibody fraction towards a linear epitope. Expected molecular weights are indicated by arrows on the right of each set of Western blots. Lysate panels for the target proteins: SYNJ2BP (Marker, RT-4), OTC (Marker, liver), TYMP (Marker, liver), CRABP2 (Marker, LY419677), WARS (Marker, RT-4, U-251 MG), PDXP (Marker, RT-4, liver), CD4 (Marker, U251-MG, plasma, tonsil), EGFR (Marker, A431).
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