FITC Annexin V 640906 from BioLegend

Product Specs
ItemFITC Annexin V
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number640906
Conjugate/TagFITC
ApplicationsFlow Cytometry (FC)
Quantity100 tests
Gene Nameannexin A5
NCBI Gene AliasesANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
NCBI Full Gene Nameannexin A5
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
TargetAnnexin V
SpeciesHuman, Mouse, Rat

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BioLegend

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Website: biolegend.com

(1) p38 Mitogen-Activated Protein Kinase Inhibition of Mesenchymal Transdifferentiated Tumor Cells in Head and Neck Squamous Cell CarcinomaBiomedicinesDecember 13, 2023Julia Federspiel, Maria do Carmo Greier, Andrea Ladányi, Jozsef DudasµL Annexin Assay Buffer (0.1 M HEPES, 0.14 M NaCl, 2.5 mM CaCl2). Five µL Annexin V-FITC (Cat. Nr. 640906) and 2 µL Podoplanin APC antibody (Cat. Nr. 337021) (both from Biolegend) were added for a 25 min incubation

(2) CD70-induced differentiation of proinflammatory Th1/17/22/GM lymphocytes associated with disease progression and immune reconstitution during HIV infectionEmerging Microbes & InfectionsDecember 8, 2023Xinyue Wang, Yuqing Wei, Zhijiao He, Di Wang, Leidan Zhang, Juan Du, Mengyuan Zhang, Meiqing Jiang, Na Chen, Meiju Deng, et al.A15153G, RRID: AB_2632931), CD95-PE-CY594 (clone DX2, RRID: AB_11153666), Annexin V-FITC (BioLegend, cat#640906), and the corresponding isotype controls. For intracellular staining of KI67-FITC (clone Ki-67, RRID:

(3) The synthetic oleanane triterpenoid CDDO-2P-Im binds GRP78/BiP to induce unfolded protein response-mediated apoptosis in myelomaMolecular oncologyDecember 1, 2023Luo G, Aldridge K, Chen T, Aslot V, Kim BG, Han EH, Singh N, Li S, Xiao TS, Sporn MB, et al.DMSO (1%) or the specified therapeutic agents for 24 h. Cells were stained with Annexin V (Catalog #640906; Biolegend, San Diego, CA, USA) and propidium iodide (Catalog #421301; Biolegend) after 24 h and analyzed

(4) Viral and host factors drive a type 1 Epstein–Barr virus spontaneous lytic phenotypemBioNovember 16, 2023Katherine A. Willard, Ashley P. Barry, Cliff I. Oduor, John Michael Ong'echa, Jeffrey A. Bailey, Ann M. Moormann, Micah A. Luftig,Santa Cruz,sc-81581 LMP1,S12,EBV,WB,Hybridoma,N/A EBNALP,JF186,EBV,WB,Hybridoma,N/A Magoh,21B12,Human,WB,Santa Cruz,sc-56724 Annexin V-FITC,N/A,Human,FACS,BioLegend,640906 LMP2A,14B7,EBV,WB,Santa Cruz,sc-101314 c-Myc,9E10,Human,WB,Santa Cruz,sc-40 SLAMF7/CD319-FITC,162.1,Human,FACS,BioLegend,331817 Ea-D,O261,EBV,FACS,Santa Cruz

(5) Water Extract of Capsella bursa-pastoris Mitigates Doxorubicin-Induced Cardiotoxicity by Upregulating Antioxidant EnzymesInternational Journal of Molecular SciencesNovember 2, 2023Yuhui Jeong, Sun-Ho Lee, Jangho Lee, Min-Sun Kim, Yu-Geon Lee, Jin-Taek Hwang, Sang-Yoon Choi, Ho-Geun Yoon, Tae-Gyu Lim, Seung-Hyun Lee, et al.cells were quantitatively analyzed by flow cytometry using Annexin V-fluorescein isothiocyanate (FITC) (640906, BioLegend, Sandiego, CA, USA), following the manufacturer’s protocol. Briefly, H9c2 cells were cultured

(6) RhoA balances microglial reactivity and survival during neuroinflammationCell Death & DiseaseOctober 20, 2023Renato Socodato, Artur Rodrigues-Santos, Joana Tedim-Moreira, Tiago O. Almeida, Teresa Canedo, Camila C. Portugal, João B. RelvasBioLegend, USA) as we did before [24, 92]. Necrotic and apoptotic microglia were determined using Annexin V (640906 BioLegend, USA) and Zombie Violet Dye (77477 BioLegend, USA). Mice were anesthetized with sodium pentobarbital

(7) Arginine dependency is a therapeutically exploitable vulnerability in chronic myeloid leukaemic stem cellsEMBO reportsOctober 9, 2023Kevin M Rattigan, Martha M Zarou, Zuzana Brabcova, Bodhayan Prasad, Désirée Zerbst, Daniele Sarnello, Eric R Kalkman, Angela Ianniciello, Mary T Scott, Karen Dunn, et al.manufacturer's instructions. Subsequently cells were stained with Annexin V (FITC or APC, BioLegend: 640906 or 640941, 5 μl/test), 7‐AAD (BD Bioscience: 559925, 5 μl/test) and CD34+ (APC, BD Bioscience: 555824,

(8) Multi-step processing of replication stress-derived nascent strand DNA gaps by MRE11 and EXO1 nucleasesNature communicationsOctober 7, 2023Hale A, Dhoonmoon A, Straka J, Nicolae CM, Moldovan GL.GloMax Navigator plate reader. Apoptosis assays were performed using the FITC Annexin V kit (Biolegend, 640906). Quantification was performed on a BD FACSCanto 10 flow cytometer using the FlowJo v10 software. DNA

(9) Ultra‐high static magnetic fields cause immunosuppression through disrupting B‐cell peripheral differentiation and negatively regulating BCR signalingMedComm (2020)October 1, 2023Heng Gu, Yufan Fu, Biao Yu, Li Luo, Danqing Kang, Miaomiao Xie, Yukai Jing, Qiuyue Chen, Xin Zhang, Juan Lai, et al.PE‐Cy7‐anti‐ki67 (1983210; Invitrogen) was used to label cells in the proliferation cycle and Annexin V (640906; BioLegend) was used to detect apoptosis. Data were acquired on Attune NxT (Thermo Fisher) and analyzed

(10) Chromatin-Associated SIN3B Protects Cancer Cells from Genotoxic Stress-Induced Apoptosis and Dictates DNA Damage Repair Pathway ChoiceMolecular cancer research : MCRSeptember 1, 2023Morales-Valencia J, Petit C, Calderon A, Saini S, David G.resuspended in 200 uL of binding buffer (BioLegend, 422201) along with 5 uL of Annexin V (BioLegend, 640906). Following 30 minutes incubation at room temperature and centrifugation, the cell pellet resuspended

(11) Inhibition of VCP modulates NF-κB signaling pathway to suppress multiple myeloma cell proliferation and osteoclast differentiationAging (Albany NY)August 30, 2023Rongfang Wei, Yuhao Cao, Hongjie Wu, Xin Liu, Mingmei Jiang, Xian Luo, Zhendong Deng, Ze Wang, Mengying Ke, Yongqiang Zhu, et al.was purchased from Beyotime (ST511, Shanghai, China), Annexin-V-FITC was purchased from Biolegend (640906, San Diego, CA, USA). Compound VCP20 was obtained from Professor Yongqiang Zhu (Nanjing Normal University). Cell

(12) Pyruvate anaplerosis is a targetable vulnerability in persistent leukaemic stem cellsNature communicationsAugust 17, 2023Rattigan KM, Brabcova Z, Sarnello D, Zarou MM, Roy K, Kwan R, de Beauchamp L, Dawson A, Ianniciello A, Khalaf A, et al.CD34 analysis Cells were stained with Annexin V (fluorescein isothiocyanate (FITC, BioLegend: Cat# 640906, 5 µL/test), 7-AAD (BD Bioscience: Cat# 559925, 5 µL/test) and CD34+ (APC, BD Bioscience, clone 581,

(13) MTH1 protects platelet mitochondria from oxidative damage and regulates platelet function and thrombosisNature communicationsAugust 10, 2023Ding Y, Gui X, Chu X, Sun Y, Zhang S, Tong H, Ju W, Li Y, Sun Z, Xu M, et al.with CRP (5 μg/ml) or thrombin (0.5 U/ml) for 30 min and then stained with FITC-Annexin-V (Biolegend, 640906, 1:20) for 15 min followed by detecting Annexin-V binding by flow cytometry. Annexin-V binding was presented

(14) Nuclear morphology predicts cell survival to cisplatin chemotherapyNeoplasiaAugust 1, 2023Chi-Ju Kim, Anna LK Gonye, Kevin Truskowski, Cheng-Fan Lee, Yoon-Kyoung Cho, Robert H. Austin, Kenneth J. Pienta, Sarah R. Amendorange channels. Flow cytometry analysis Live cells were incubated with a FITC-Annexin V (BioLegend, #640906) according to the manufacturer's recommendations. Cells were analyzed on an Attune NxT Flow Cytometer

(15) In silico enhancer mining reveals SNS-032 and EHMT2 inhibitors as therapeutic candidates in high-grade serous ovarian cancerBritish Journal of CancerJuly 27, 2023Marcos Quintela, David W. James, Jetzabel Garcia, Kadie Edwards, Lavinia Margarit, Nagindra Das, Kerryn Lutchman-Singh, Amy L. Beynon, Inmaculada Rioja, Rab K. Prinjha, et al.bars represent the coefficient of variation. Apoptosis staining was performed using Annexin V-FITC (640906, Biolegend; CA, US) and Propidium iodide (PI, 421301, Biolegend) as per manufacturer’s instructions,

(16) EHBP1L1, an apicobasal polarity regulator, is critical for nuclear polarization during enucleation of erythroblastsBlood AdvancesJuly 25, 2023Ji Wu, Kenta Moriwaki, Tatsuya Asuka, Ritsuko Nakai, Satoshi Kanda, Manabu Taniguchi, Tatsuki Sugiyama, Shin-ichiro Yoshimura, Masataka Kunii, Takashi Nagasawa, et al.N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid, 140 mM NaCl, and 2.5 mM CaCl2) and then stained with FITC-conjugated Annexin V (BioLegend, 640906). The fluorescently labeled antibodies are listed in supplemental Table 1. For intracellular staining

(17) Metabolic Profiles Point Out Metabolic Pathways Pivotal in Two Glioblastoma (GBM) Cell Lines, U251 and U-87MGBiomedicinesJuly 20, 2023Filipa Martins, David van der Kellen, Luís G. Gonçalves, Jacinta SerpaThen, cell pellets were incubated with 0.5 µL annexin V-fluorescein isothiocyanate (annexin V-FITC; 640906, BioLegend, San Diego, CA, USA), in annexin V binding buffer 1× (10 mM HEPES (pH 7.4; 391333, Millipore,

(18) Optogenetic Induction of Pyroptosis, Necroptosis, and Apoptosis in Mammalian Cell LinesBIO-PROTOCOLJuly 20, 2023Kateryna Shkarina, Petr Broz424001)Annexin V Pacific Blue (BioLegend, catalog number: 640918)Annexin V FITC (BioLegend, catalog number: 640906)Annexin V Alexa Fluor 647 (BioLegend, catalog number: 640912)CellEvent caspase-3/7 green (Thermo Fisher Scientific

(19) A localized hydrogel-mediated chemotherapy causes immunogenic cell death via activation of ceramide-mediated unfolded protein responseScience advancesJune 30, 2023Kar A, Jain D, Kumar S, Rajput K, Pal S, Rana K, Kar R, Jha SK, Medatwal N, Yavvari PS, et al.Allprotect tissue reagent (#76405) was purchased from QIAGEN, Hilden, Germany. Annexin-FITC assay kit (#640906) was purchased from BioLegend, San Diego, USA. Developer solution (#4908216) and XBT x-ray film (#6568307)

(20) miR-216b-5p promotes late apoptosis/necroptosis in trastuzumab-resistant SK-BR-3 cellsTurkish Journal of BiologyMay 23, 2023İŞTAR BURCU DOLAPÇI, SENEM NOYAN, AYŞEGÜL YÜCEL POLAT, HAKAN GÜRDAL, BALA GÜR DEDEOĞLUpH: 7.4; 140 mM NaCl, 2.5 mM CaCl2) in a concentration of 1 × 106 cells/mL. Five μL Annexin V-FITC (640906, BioLegend) and five μL PI (421301, BioLegend) (0.5 μg/μL) were added and the cells were incubated in

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1/6/2015Measurement of Differential Apoptosis of HEK cells in presence of PDGF Anirban BanerjeeApoptosis of keratinocytes is a crucial step that balances proliferation to maintain proper epidermal thickness. However keratinocytes from the peripheral area of chronic wounds undergo higher and ... Read Review

FITC Annexin V from BioLegend

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FITC Annexin V from BioLegend

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