Alexa Fluor(R) 647 Annexin V 640912 from BioLegend

Product Specs
ItemAlexa Fluor(R) 647 Annexin V
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number640912
Conjugate/TagAlexa Fluor® 647
ApplicationsFlow Cytometry (FC)
Quantity100 tests
Gene Nameannexin A5
NCBI Gene AliasesANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
NCBI Full Gene Nameannexin A5
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
TargetAnnexin V
SpeciesHuman, Mouse, Rat

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BioLegend

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San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) A transcriptional network governing ceramide homeostasis establishes a cytokine-dependent developmental processNature CommunicationsNovember 9, 2023Ruiqi Liao, Abiola Babatunde, Stephanie Qiu, Hamsini Harikumar, Joshua J. Coon, Katherine A. Overmyer, Yusuf A. Hannun, Chiara Luberto, Emery H. Bresnick140 mM NaCl, 2.5 mM CaCl2, pH 7.4). Cells were stained with Alexa Fluor® 647-Annexin V (BioLegend, 640912, 1:40) in 100 μl Annexin V binding buffer and incubated at RT for 15 min. Cells were then washed with

(2) Short-term assays for mesenchymal stromal cell immunosuppression of T-lymphocytesFrontiers in ImmunologySeptember 26, 2023Maryanne C. Herzig, Barbara A. Christy, Robbie K. Montgomery, Carolina Cantu-Garza, Gema D. Barrera, Ji H. Lee, Nicholas Mucha, Jennifer R. Talackine, Isaac A. Abaasah, James A. Bynum, et al.Cat#304029 RRID : AB_2174123, San Diego, CA) and 5 µL of Alexa Fluor® 647 Annexin V (BioLegend Cat # 640912, San Diego, CA) with and without 1 µL of FITC-bovine lactadherin (Prolytix, Essence Junction, VT) for

(3) Actionable loss of SLF2 drives B‐cell lymphomagenesis and impairs the DNA damage responseEMBO Molecular MedicineSeptember 11, 2023Le Zhang, Matthias Wirth, Upayan Patra, Jacob Stroh, Konstandina Isaakidis, Leonie Rieger, Susanne Kossatz, Maja Milanovic, Chuanbing Zang, Uta Demel, et al.Aqua Dead Cell Stain (Invitrogen, L34957)/Annexin‐V staining (Alexa Fluor® 647 Annexin V, BioLegend, 640912). Data were acquired using a CytoFLEX S Flow Cytometer (Beckman Coulter) and analyzed with FlowJo™ Version

(4) Optogenetic Induction of Pyroptosis, Necroptosis, and Apoptosis in Mammalian Cell LinesBIO-PROTOCOLJuly 20, 2023Kateryna Shkarina, Petr Broz640918)Annexin V FITC (BioLegend, catalog number: 640906)Annexin V Alexa Fluor 647 (BioLegend, catalog number: 640912)CellEvent caspase-3/7 green (Thermo Fisher Scientific, catalog number: R37111) Cell lysis and cytokine

(5) NUAK1 governs centrosome replication in pancreatic cancer via MYPT1/PP1β and GSK3β‐dependent regulation of PLK4Molecular OncologyJuly 1, 2023Declan Whyte, George Skalka, Peter Walsh, Ania Wilczynska, Nikki R. Paul, Claire Mitchell, Colin Nixon, William Clarke, Martin Bushell, Jennifer P. Morton, et al.(10 mm Hepes pH 7.4, 140 mm NaCl, 2.5 CaCl2) containing 2 μL of Alexa Flour 647 Annexin V (BioLegend 640912, San Diego, CA, USA). The cells were left in the dark for 10 min at room temperature. Prior to analysis

(6) A rationally designed fluorescence probe achieves highly specific and long‐term detection of senescence in vitro and in vivoAging CellJune 13, 2023Li Hu, Chanjuan Dong, Zhe Wang, Shengyuan He, Yiwen Yang, Meiting Zi, Huiqin Li, Yanghuan Zhang, Chuanjie Chen, Runzi Zheng, et al.tubes (352058, Falcon, Corning, NY, USA). The cells were stained with Alexa Fluor 647‐Annexin V (1:60, 640912, BioLegend, San Diego, CA, USA) and propidium iodide (PI, 1:60, 00‐6990, eBioscience, CA, USA) at room

(7) Dasatinib overcomes glucocorticoid resistance in B-cell acute lymphoblastic leukemiaNature communicationsMay 22, 2023Sarno J, Domizi P, Liu Y, Merchant M, Pedersen CB, Jedoui D, Jager A, Nolan GP, Gaipa G, Bendall SC, et al.combinations (n = 36) for 48 h before being stained with Annexin V-APC Apoptosis Detection kit (Biolegend 640912, 420403, and 4220010) following manufacturer instructions and acquired on CytoFLEX Flow Cytometer (Beckman Coulter

(8) Myd88 Signaling Is Involved in the Inflammatory Response in LPS-Induced Mouse Epididymitis and Bone-Marrow-Derived Dendritic CellsInternational Journal of Molecular SciencesApril 25, 2023Jin-Chuan Liu, Peng Wang, Qun-Xiong Zeng, Chen Yang, Minmin Lyu, Yanfeng Li, William Shu-Biu Yeung, Philip Chi-Ngong Chiu, Gerhard Haidl, Jean-Pierre Allam, et al.once with PBS, the BMDCs were then stained with Annexin V (Biolegend, San Diego, CA, USA, Cat. No. 640912) at room temperature for 15 min and were washed once with Annexin V binding buffer. Finally, the BMDCs

(9) Naturally derived cytokine peptides limit virus replication and severe disease during influenza A virus infectionClinical & Translational ImmunologyMarch 23, 2023Christopher M Harpur, Alison C West, Mélanie A Le Page, Maggie Lam, Christopher Hodges, Osezua Oseghale, Andrew J Gearing, Michelle D Tateand CD11c (clone HL3, Cat #553801, RRID:AB_396683). Cells were then incubated with Annexin V (Cat #640912; BioLegend, San Diego, USA) in binding buffer (10 mM HEPES pH 7.4, 150 mM NaCl, and 2.5 mM CaCl2) and

(10) Nanoscale imaging of CD47 informs how plasma membrane modifications shape apoptotic cell recognitionCommunications BiologyFebruary 22, 2023Samy Dufour, Pascale Tacnet-Delorme, Jean-Philippe Kleman, Oleksandr Glushonkov, Nicole Thielens, Dominique Bourgeois, Philippe Frachetnext imaged in DMEM Fluorobrite supplemented with Annexin V labeled with Alexa Fluor 647 (Biolegend, 640912, 2.5 µg/mL) to detect exposed phosphatidylserine. Cells were visualized 4 h after irradiation with a

(11) SARS-CoV-2 RBD and Its Variants Can Induce Platelet Activation and Clearance: Implications for Antibody Therapy and Vaccinations against COVID-19Research (Washington, D.C.)January 1, 2023Ma X, Liang J, Zhu G, Bhoria P, Shoara AA, MacKeigan DT, Khoury CJ, Slavkovic S, Lin L, Karakas D, et al.human platelets. Annexin V, fibrinogen, and RCA-1 binding were detected with Alexa Fluor 647 Annexin V (640912, Biolegend), Alexa Fluor 647 Fibrinogen (F35200, Thermo Fisher Scientific) and fluorescein labeled RCA-1

(12) Microfluidic-based dynamic BH3 profiling predicts anticancer treatment efficacynpj Precision OncologyDecember 1, 2022Albert Manzano-Muñoz, José Yeste, María A. Ortega, Fernando Martín, Anna López, Jordi Rosell, Sandra Castro, César Serrano, Josep Samitier, Javier Ramón-Azcón, et al.acid, 40 mM KCl, 1.4 M NaCl, 7.5 mM MgCl2, and 25 mM CaCl2 at pH 7.4) with Annexin V- Alexa Fluor 647® (640912, BioLegend) and DAPI (62248, ThermoFisher). Results were obtained in a Gallios flow cytometer (Beckman Coulter

(13) Distinct roles of ORAI1 in T cell–mediated allergic airway inflammation and immunity to influenza A virus infectionScience AdvancesOctober 7, 2022Yin-Hu Wang, Lucile Noyer, Sascha Kahlfuss, Dimitrius Raphael, Anthony Y. Tao, Ulrike Kaufmann, Jingjie Zhu, Marisa Mitchell-Flack, Ikjot Sidhu, Fang Zhou, et al.3 and 4 days. Cells were then stained for surface markers and annexin V–Alexa Fluor 647 (BioLegend, 640912) in annexin V staining buffer (BioLegend, 422201) for 10 min and analyzed by flow cytometry. For cell

(14) A dual role for the RNA helicase DHX34 in NMD and pre-mRNA splicing and its function in hematopoietic differentiationRNA (New York, N.Y.)September 1, 2022Hug N, Aitken S, Longman D, Raab M, Armes H, Mann AR, Rio-Machin A, Fitzgibbon J, Rouault-Pierre K, Cáceres JF.assess cell cycle upon expansion conditions at day 14. An Alexa Fluor 647 Annexin V (BioLegend, cat. no. 640912) was used with Annexin V Binding Buffer (BD Bioscience, cat. no. 556454) to measure apoptosis at day

(15) Mitochondrial ROS promotes susceptibility to infection via gasdermin D-mediated necroptosisCellAugust 18, 2022Weindel CG, Martinez EL, Zhao X, Mabry CJ, Bell SL, Vail KJ, Coleman AK, VanPortfliet JJ, Zhao B, Wagner AR, et al.,Thermo Fisher,C10444 TMRE,Invitrogen,11560796 JC-1; CB1C2,Invitrogen,T3168 Propidium Iodide,Invitrogen,P1304MP Annexin-V AF647,Biolegend,640912 Annexin-V FITC,Biolegend,640906 Annexin binding buffer,Biolegend,422201 etoposide,Fisher,E0675 ABT737,ChemCruz,Sc-207242 Mdivi-1,abcam,Ab144589 "Nigericin, free acid

(16) Endoplasmic reticulum stress-induced release and binding of calreticulin from human ovarian cancer cellsCancer immunology, immunotherapy : CIIJuly 1, 2022Abdullah TM, Whatmore J, Bremer E, Slibinskas R, Michalak M, Eggleton P.(Biolegend—PN-422201) and 100 μl aliquots of cells co-stained with 5 μl Alexa Fluor® 647 Annexin V ((Biolegend—640912) to monitor apoptosis and 10 μl propidium iodide-Yellow (Sigma-81845) for 15 min in the dark to assess

(17) The HIFα-Stabilizing Drug Roxadustat Increases the Number of Renal Epo-Producing Sca-1⁺ CellsCellsFebruary 21, 2022Jatho A, Zieseniss A, Brechtel-Curth K, Guo J, Böker KO, Salinas G, Wenger RH, Katschinski DM.

(18) Modulated Electro-Hyperthermia Supports the Effect of Gemcitabine Both in Sensitive and Resistant Pancreas Adenocarcinoma Cell LinesPathology and Oncology ResearchDecember 10, 2021Gertrud Forika, Eva Kiss, Gabor Petovari, Titanilla Danko, Aron Bertram Gellert, Tibor Krenacsapoptosis analysis. For apoptosis measurement, cells were stained with Alexa Fluor 647 Annexin V (1:100; #640912, BioLegend, San Diego, CA, United States) and 1 µl of 1 mg/ml stock solution (diluted in PBS) of propidium

(19) Tnfrsf4-expressing regulatory T cells promote immune escape of chronic myeloid leukemia stem cellsJCI insightDecember 8, 2021Hinterbrandner M, Rubino V, Stoll C, Forster S, Schnüriger N, Radpour R, Baerlocher GM, Ochsenbein AF, Riether C.

(20) Opposing Effects of Chelidonine on Tyrosine and Serine Phosphorylation of STAT3 in Human Uveal Melanoma CellsInternational Journal of Molecular SciencesNovember 30, 2021István Csomós, Péter Nagy, Csenge Filep, István Rebenku, Enikő Nizsalóczki, Tamás Kovács, György Vámosi, László Mátyus, Andrea Bodnár

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Alexa Fluor(R) 647 Annexin V from BioLegend

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Alexa Fluor(R) 647 Annexin V from BioLegend

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