Adenosine-5 Triphosphate (ATP) P0756L from New England Biolabs

Product Specs
ItemAdenosine-5 Triphosphate (ATP)
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberP0756L
Quantity5 ml
Molecule NameAdenosine 5'-Triphosphate (ATP)
ApplicationsProteomics & Glycomics
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Inferring phylogenetic structure, taxa hybridization, and divergence times within rock voles of subgenus Aschizomys (Cricetidae: Alticola) using quaddRAD sequencing and a cytb datasetEcol EvolDecember 13, 2023Ivan A. Dvoyashov, Semyon Yu. Bodrov, Nikolai V. Mamaev, Elena S. Glagoleva, Natalia I. AbramsonNew England Biolabs), 0.6 μL of T4 ligase (400 U/μL) (cat. # M0202S, New England Biolabs), 6 μL of 10 mM ATP (cat. # P0756S, New England Biolabs), 0.7 μL of the quaddRAD‐i5 adapter, 0.7 μL of the quaddRAD‐i7 adapter, and nuclease‐free

(2) A group B Streptococcus indexed transposon mutant library to accelerate genetic research on an important perinatal pathogenMicrobiology SpectrumDecember 12, 2023Venkata H. Bhavana, Gideon H. Hillebrand, Kathyayini P. Gopalakrishna, Rebekah A. Rapp, Adam J. Ratner, Hervé Tettelin, Thomas A. HoovenDNA was combined with T4 ligase (New England Biolabs cat. # M0202L), ATP (New England Biolabs cat. # P0756L), and a double-stranded, well-barcoded adapter. This reaction was allowed to proceed overnight at room

(3) SLC3A2 N-glycosylation and Golgi remodeling regulate SLC7A amino acid exchangers and stress mitigationJournal of Biological ChemistryDecember 1, 2023Cunjie Zhang, Massiullah Shafaq-Zadah, Judy Pawling, Geoffrey G. Hesketh, Estelle Dransart, Karina Pacholczyk, Joseph Longo, Anne-Claude Gingras, Linda Z. Penn, Ludger Johannes, et al.FastDigest BbsI (#FD1014) from Thermo Scientific; T7 DNA ligase (#M0318S), T4 PNK (#M0201S), ATP (#P0756S) from NEB; Plasmid-Safe ATP-Dependent DNase (#E3101K) from Epicentre; PolyJet reagent from SignaGen

(4) IsoSeek for unbiased and UMI-informed sequencing of miRNAs from low input samples at single-nucleotide resolutionSTAR ProtocolsDecember 1, 2023Monique A.J. van Eijndhoven, Chantal Scheepbouwer, Ernesto Aparicio-Puerta, Michael Hackenberg, D. Michiel Pegtelprocedure after ligation of the 3′-5N-adapter in the presence of 10 mM ATP (New England Biolabs, Cat#P0756S) and T4 DNA ligase (New England Biolabs, Cat#M0202T). Samples were incubated for 1 h at 30°C and 10

(5) The neuronal calcium sensor NCS-1 regulates the phosphorylation state and activity of the Ga chaperone and GEF Ric-8AeLifeNovember 29, 2023Daniel Muñoz-Reyes, Levi J McClelland, Sandra Arroyo-Urea, Sonia Sánchez-Yepes, Juan Sabín, Sara Pérez-Suárez, Margarita Menendez, Alicia Mansilla, Javier García-Nafría, Stephen Sprang, et al.protein",Ric-8A P3 peptide (400-432),GenicBio,N/A, "Chemical compound, drug",ATP,New England Biolabs,Cat # P0756S, "Chemical compound, drug",Water for UHPLC-MS LiChrosolv,Merck,Cat # 1037282002, "Chemical compound,

(6) Preparation and processing of dried blood spots for microRNA sequencingBiology Methods and ProtocolsSeptember 20, 2023Alice Morgunova, Pascal Ibrahim, Gary Gang Chen, Saché M Coury, Gustavo Turecki, Michael J Meaney, Anthony Gifuni, Ian H Gotlib, Corina Nagy, Tiffany C Ho, et al.(Promega M3011)5′ deadenylase (NEB M0331)RecJf (NEB M0264) Reagents for 5′-ligation 10 mM ATP (NEB P0756)T4 RNA ligase 1 (NEB M0204) Reagents for reverse transcription Strip tubes (Axygen PCR-0208-CP-C or

(7) An efficient cloning method to expand vector and restriction site compatibility of Golden Gate AssemblyCell Reports MethodsAugust 22, 2023Masato Sorida, Roberto Bonasio,Thermo Scientific,SM1331 BsaI-HFv2,NEB,R3733S BbsI-HF,NEB,R3539S AgeI-HF,NEB,R3552S BamHI-HF,NEB,R3136S EcoRI-HF,NEB,R3101L HindIII-HF,NEB,R3104S NcoI-HF,NEB,R3193S NotI-HF,NEB,R3189S SalI-HF,NEB,R3138S SpeI-HF,NEB,R3133S XbaI,NEB,R0145S XhoI,NEB,R0146S XmaI,NEB,R0180S KpnI-HF,NEB,R3142S NdeI,NEB,R0111S Smal,NEB,R0141S BbsI-HF,NEB,R3539M T4 DNA Ligase,NEB,M0202L Hi-T4 DNA Ligase,NEB,M2622S 100 mM ATP,NEB,P0756S NEBuilder HiFi DNA Assembly Master Mix,NEB,E2621 LB Broth,Fisher Scientific,BP9723 Agar,Fisher Scientific,BP1423 OneTaq

(8) Specific Regulation of m(6)A by SRSF7 Promotes the Progression of GlioblastomaGenomics, proteomics & bioinformaticsAugust 1, 2023Cun Y, An S, Zheng H, Lan J, Chen W, Luo W, Yao C, Li X, Huang X, Sun X, et al.No. M0375S, NEB), 0.01 U Bst 2.0 DNA polymerase (Catalog No. M0537S, NEB), and 10 nM ATP (Catalog No. P0756L, NEB) was added to a final volume of 20 μl and incubated at 40 °C for 20 min, denatured at 80 °C for

(9) Systematic mapping and modeling of 3D enhancer-promoter interactions in early mouse embryonic lineages reveal regulatory principles that determine the levels and cell-type specificity of gene expressionbioRxivJuly 25, 2023Dylan Murphy, Eralda Salataj, Dafne Campigli Di Giammartino, Javier Rodriguez-Hernaez, Andreas Kloetgen, Vidur Garg, Erin Char, Christopher M. Uyehara, Ly-sha Ee, UkJin Lee, et al.the samples in 669 μL ddH2O and adding 120 μL T4 ligation buffer (NEB, B0202), 60 μL 10mM ATP (NEB, P0756S), 120 μL 10% Triton X-100, 6 μL 20mg/ml BSA and 5 μL 400U/μl T4 DNA Ligase (NEB, M0202) overnight at

(10) ULK phosphorylation of STX17 controls autophagosome maturation via FLNAJ Cell BiolJune 30, 2023Yufen Wang, Huilin Que, ChuangPeng Li, Zhe Wu, Fenglei Jian, Yuan Zhao, Haohao Tang, Yang Chen, Shuaixin Gao, Catherine C.L. Wong, et al.Latrunculin B (Cat#MZ5810, Lot#2606Z210844) was purchased from MKBIO. Lambda PP (Cat#P0753L), and ATP (Cat#P0756s, Lot#10153222) were purchased from NEB. Rabbit polyclonal anti-STX17 (Cat#17815-1-AP, Lot#00097453,

(11) High-resolution spatial multi-omics reveals cell-type specific nuclear compartmentsbioRxivMay 12, 2023Yodai Takei, Yujing Yang, Jonathan White, Jina Yun, Meera Prasad, Lincoln J Ombelets, Simone Schindler, Long CaiLigase Reaction Buffer from Quick Ligation Kit (NEB M2200) supplemented with additional 1 mM ATP (NEB P0756) at room temperature for 1 hour. The samples were then washed with the 12.5% wash buffer and rinsed

(12) Combinatorial quantification of 5mC and 5hmC at individual CpG dyads and the transcriptome in single cells reveals modulators of DNA methylation maintenance fidelitybioRxivMay 11, 2023Alex Chialastri, Saumya Sarkar, Elizabeth E. Schauer, Shyl Lamba, Siddharth S. Deydouble-stranded adapter was added. Then 9 µL of ligation mix (1.11x T4 ligase reaction buffer, 4.44 mM ATP (NEB, P0756L), 2000 U T4 DNA ligase (NEB, M0202M)) was added to each sample, and the samples were incubated at 16°C

(13) Targeted Demethylation of the TGFβ1 mRNA Promotes Myoblast Proliferation via Activating the SMAD2 Signaling PathwayCellsMarch 24, 2023Kaiping Deng, Zhipeng Liu, Xiaodan Li, Zhen Zhang, Yixuan Fan, Qunhao Huang, Yanli Zhang, Feng WangBst 2.0 DNA polymerase (NEB, #M0537S), 0.5 U SplintR ligase (NEB, #M0375S), and 10 nmol ATP (NEB, #P0756S), and then were incubated at 40 °C for 20 min, denatured at 80 °C for 20 min. Afterward, 2 μL of the

(14) A High-Throughput Strategy for T-Cell Receptor Cloning and ExpressionMethods in Molecular BiologyMarch 6, 2023Qiong Xia, Huang Huang, Mark M. Davis(NEW ENGLAND BIOLABS, Cat. no. M0202). Adenosine 5’-Triphosphate (ATP) (NEW ENGLAND BIOLABS, Cat. no. P0756). BbsI-HF® (NEW ENGLAND BIOLABS, Cat. no. R3539). UltraPure DNase/RNase-free distilled water (Life Technologies

(15) Assessment of microbiota in the gut and upper respiratory tract associated with SARS-CoV-2 infectionMicrobiomeMarch 3, 2023Jiarui Li, Qiuyu Jing, Jie Li, Mingxi Hua, Lin Di, Chuan Song, Yanyi Huang, Jianbin Wang, Chen Chen, Angela Ruohao WuMgCl2, 10% DMF) supplemented with 3.4% PEG8000 (VWR Life Science, Cat.No.97061), 1 mM ATP (NEB,Cat.No. P0756), and 1U/μl RNase inhibitor (TaKaRa, Cat.No. 2313B). The reaction was incubated at 55°C for 30 min.

(16) A TurboID-based proximity labelling approach for identifying the DNA-binding proteinsSTAR ProtocolsMarch 1, 2023Xia-fei Wei, Shan Li, Jie-li Hupeptides, and recombinant proteins" ,, DTT,Thermo Scientific,Cat#D1532 ATP,New England Biolabs,Cat#P0756S Tango buffer (10×),Thermo Scientific,Cat#BY5 BsmbI-V2,New England Biolabs,Cat#R0739S T7 DNA ligase,New England Biolabs

(17) Paternal phthalate exposure-elicited offspring metabolic disorders are associated with altered sperm small RNAs in miceEnvironment InternationalFebruary 1, 2023Jingwei Liu, Junchao Shi, Rebecca Hernandez, Xiuchun Li, Pranav Konchadi, Yuma Miyake, Qi Chen, Tong Zhou, Changcheng ZhouμL) containing 10 × PNK buffer (5 μL, New England Biolabs, B0201S), ATP (1 mM, New England Biolabs, P0756S), T4PNK (10 U, New England Biolabs, M0201L) and incubated for 20 min (37 °C). 500 μL TRIzol was added

(18) CK2-Mediated Phosphorylation Upregulates the Stability of USP13 and Promotes Ovarian Cancer Cell ProliferationCancers (Basel)December 29, 2022Juntae Kwon, Jinmin Zhang, Boram Mok, Cecil Hanby Coomassie brilliant blue staining. Active CK2 complex (P6010S), kinase buffer (B6022), and ATP (P0756S) were purchased from NEB (New England Biolabs, Ipswich, MA). The same amount of recombinant GST and

(19) Engineering inducible biomolecular assemblies for genome imaging and manipulation in living cellsNature CommunicationsDecember 24, 2022Qin Peng, Ziliang Huang, Kun Sun, Yahan Liu, Chi Woo Yoon, Reed E. S. Harrison, Danielle L. Schmitt, Linshan Zhu, Yiqian Wu, Ipek Tasan, et al.100 ng pLH-sgRNA1-MS2-PP7, 1 μl 10 x CutSmart Buffer (B7204, New England BioLabs), 1 μl 10 mM ATP (P0756S, New England BioLabs), 0.5 μl BbsI (R3539S, New England BioLabs), 0.3 μl T7 DNA ligase (M0318S, New England BioLabs

(20) Efficient modification and preparation of circular DNA for expression in cell cultureCommunications BiologyDecember 21, 2022Roman Teo Oliynyk, George M. ChurchBuffer (10X B0202S),5.0, T4 DNA Ligase (NEB M0202L),3.0, Bsai-HF2 (NEB R3733L),3.0, ATP (10mM NEB P0756S),5.0, Nuclease-free H2O (Invitrogen 10977015),as needed to 50.0 μl, Cycler schedule,Cycler schedule,Cycler

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Adenosine-5 Triphosphate (ATP)

Adenosine-5 Triphosphate (ATP) from New England Biolabs

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New England Biolabs

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Adenosine-5 Triphosphate (ATP) from New England Biolabs

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