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Endoproteinase AspN
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Endoproteinase AspN
Endoproteinase AspN from New England Biolabs
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Citations:
(7)
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Endoproteinase AspN
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Description
Endoproteinase AspN (flavastacin) is a zinc metalloendopeptidase which selectively cleaves peptide bonds N-terminal to aspartic acid residues.
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Endoproteinase AspN
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
P8104S
Molecule Name
Endoproteinase AspN
Quantity
50 µg
Applications
Proteomics & Glycomics
Brand/Trademark
New England Biolabs Inc (NEB)
Temperature
-20 C
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (7)
(1)
Amyloid fibril proteomics of AD brains reveals modifiers of aggregation and toxicity
Molecular Neurodegeneration
September 14, 2023
Arun Upadhyay, Deepak Chhangani, Nalini R. Rao, Julia Kofler, Robert Vassar, Diego E. Rincon-Limas, Jeffrey N. Savas
α-chymotrypsin (Sigma, Cat#C3142), thermolysin (Sigma, Cat#P1512), endoproteinase Asp-N (
New England Biolabs
, #
P8104S
), Glu-C (
Sigma
, Cat#
P2922
), Arg-C (
Biovendor
, Cat#
RBG40003005
), trypsin (
Promega
, Cat#
V5280
), and Lys-C
(2)
Placental Remote Control of Fetal Metabolism: Trophoblast mTOR Signaling Regulates Liver IGFBP-1 Phosphorylation and IGF-1 Bioavailability
International Journal of Molecular Sciences
April 14, 2023
Fredrick J. Rosario, Anand Chopra, Kyle Biggar, Theresa L. Powell, Madhulika B. Gupta, Thomas Jansson
was then diluted with 2X
Asp
-N reaction buffer (
NEB
,
B8104S
) and digested with 10 ng of
Asp
-N (
NEB
,
P8104S
) overnight at 37 °C. The resultant peptides were desalted with C18-ZipTip (
Millipore Sigma
,
ZTC18S096
),
(3)
Site-specific N-glycosylation analysis of animal cell culture-derived Zika virus proteins
Scientific Reports
December 1, 2021
Alexander Pralow, Alexander Nikolay, Arnaud Leon, Yvonne Genzel, Erdmann Rapp, Udo Reichl
Sequencing Grade Modified,
V5111
,
Promega
, Madison, WI, USA)
endoproteinase AspN
[ Flavastacin20] (AspN,
P8104S
,
New England Biolabs
, Ipswich, MA, USA),
proteinase K
(A4392, AppliChem, Darmstadt, Germany) and peptide
(4)
Unexpected Receptor Functional Mimicry Elucidates Activation of Coronavirus Fusion
Cell
February 1, 2019
Alexandra C. Walls et al.
TG269188 Chymotrypsin,Sigma-Aldrich,Cat# 9004-07-3 Alphalytic
Protease
,
Sigma-Aldrich
,Cat# A6362 AspN Endoproteinase,
NEB
,Cat#
P8104S
EndoH,
NEB
,Cat#
P0702S
Lot# 0181603 EndoF3,CalBIOCHEM,Cat# 324727 Lot# 2754801
Proteinase K
,
ThermoScientific
,Cat#
(5)
Improvement of the glycoproteomic toolbox with the discovery of a unique C-terminal cleavage specificity of flavastacin for N-glycosylated asparagine
Scientific Reports
December 1, 2017
Alexander Pralow, Marcus Hoffmann, Terry Nguyen-Khuong, Erdmann Rapp, Udo Reichl
were trypsin (Trypsin Sequencing Grade Modified;
V5111
) from
Promega
and
endoproteinase AspN
(AspN;
P8104S
) from
New England Biolabs
(Flavastacin, purified host cell protein, Uniprot Q47899; see the corresponding
(6)
Assessing in vivo dynamics of multiple quality attributes from a therapeutic IgG4 monoclonal antibody circulating in cynomolgus monkey
mAbs
July 3, 2016
Yinyin Li, Yu Huang, Janine Ferrant, Yelena Lyubarskaya, Yue (Emma) Zhang, Siyang (Peter) Li, Shiaw-Lin (Billy) Wu
purchased from MP Biomedicals (Solon, OH);
Endoproteinase AspN
was purchased from
New England BioLabs
Inc. (
P8104S
) and
Roche Diagnostics
(
11054589001
). Dithiothreitol (DTT, D5545), iodoacetamide (IAA,
I6125
) and N-ethylmaleimide
(7)
Enzyme-Induced Formation of β-Lactoglobulin Fibrils by AspN Endoproteinase
Food Biophysics
December 1, 2008
Cynthia Akkermans, Paul Venema, Atze Jan van der Goot, Remko M. Boom, Erik van der Linden
Biophysics (2008) 3:390–394 50 mM Tris–HCl and 2 mM zinc acetate. The enzyme AspN endoproteinase (
P8104S
,
New England Biolabs
, Ipswich, MA, U.S.A.) was dissolved in Millipore water (0.17 mg/ml) and was added
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Endoproteinase AspN from New England Biolabs
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