Fig 1: Characterization of EV-preparations. Plasma EV-preparations from patients and HBDs containing EVs isolated by the Exo (RN) easy kit (Qiagen) were characterized by NTA (a,b), immune-assays (c-e), and TEM (f, g)). Figures a-b demonstrate differences in nanoparticle concentration (a) and size (b) between healthy blood donors (HBD) and patients (PT). Figures C-E illustrate the EV protein content measured for EpCAM, APOB and FLOT-1. EV-preparation of patients have more EpCAM but less APOB compared to HBDs. The EV cytosolic protein FLOT-1 was comparable between both EV-preparations. Figures f and g shows EVs, indicated by arrows, as visualized by TEM from EV-preparations of patient 15 plasma. Pictures were taken at 11 k (f) and 45 k (g) magnification