Fig 1: (A) Blots of Iba1, P38 MAPK, and PP38 MAPK in primary microglia. (B) Relative protein levels of Iba1 in primary microglia. (C) Relative protein levels of P38 MAPK Iba1 in primary microglia. (D) Relative protein levels of PP38 MAPK in primary microglia [*Compare with Con-MG, P < 0.05, **Compare with Con-MG, P < 0.01.Con-MG (n = 3), LCN2-MG (n = 3)].
Fig 2: (A) Serum LCN2 level in the three groups. (B) Relative LCN2 mRNA levels in the hippocampal region in the three groups. (C) Relative LCN2 mRNA levels in different brain regions in the PSD group. (D) Relative LCN2 mRNA levels in different brain regions in the PS group. (E) Correlation of serum LCN2 level with the horizontal score of open field 2 weeks postoperation. (F) Correlation of serum LCN2 level with the self-grooming score of open field 3 weeks post operation. [#Compare with the PS group, P < 0.05, ##Compare with the PS group, P < 0.01; *Compare with the Sham group, P < 0.05, **Compare with the Sham group, P < 0.01; ▲▲Compare with post operation 1 week, P < 0.01; ∙Compare with post operation 2 weeks, P < 0.05, ∙∙Compare with post operation 2 weeks, P < 0.01; ⋄Compare with the right cerebral hemisphere, P < 0.05; △ Compare with the left cerebral hemisphere, P < 0.05, △ △ Compare with the Left cerebral Hemisphere, P < 0.01; Sham group (n = 6), PS group (n = 7), and PSD group (n = 10)].
Fig 3: (A) NO level in the supernatant of primary microglia. (B) Length of primary microglia. (C) Migration ability of primary microglia. (D) Phagocytic ability of primary microglia. (E) Uptake of 1 ml microspheres for 5 h by Con-MG. (F) Uptake of 1 ml microspheres for 5 h by LCN2-MG. (Primary microglia were identified by Iba1 expression (green), and nuclei were stained with DAPI (blue). Scale bars: 20 µm. [**Compare with Con-MG, P < 0.01. Con-MG (n = 3), LCN2-MG (n = 3)].
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