Streptavidin (HRP) ab7403 from Abcam

Product Specs
ItemStreptavidin (HRP)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab7403
Quantity500 UG
ApplicationsDot, ELISA, ICC, IHC-Fr, IHC-P, IM, WB
Concentration1 mg/ml
TypeNative
Source/Expression SystemNative
ActivityActive
Molecule NameStreptavidin

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Subunit nanovaccine elicited T cell functional activation controls Trypanosoma cruzi mediated maternal and placental tissue damage and improves pregnancy outcomes in miceNPJ VaccinesDecember 16, 2023Lizette Elaine Rios, Nandadeva Lokugamage, Subhadip Choudhuri, Imran Hussain Chowdhury, Nisha Jain Gargab97265, Abcam) for 1 h and streptavidin-horse radish peroxidase (HRP) conjugate (1:10,000 dilution, ab7403, Abcam) for 30 min. IgG1: goat anti-mouse IgG1 (1:1000 dilution, ab97236, Abcam) for 1 h, donkey anti-goat

(2) Proximity Interactome Analysis of Super Conserved Receptors Expressed in the Brain Identifies EPB41L2, SLC3A2, and LRBA as Main PartnersCellsNovember 14, 2023Abeer Kaafarani, Romain Darche-Gabinaud, Xavier Bisteau, Virginie Imbault, Valérie Wittamer, Marc Parmentier, Isabelle Pirsonanti-rabbit IgG (#A-21206, Invitrogen, Waltham, MA, USA, 1:500). The HRP-conjugated streptavidin was #ab7403 ( Abcam, Cambridge, UK, WB 1:4000). 2.2. DNA Constructs A construct encoding a fusion of human SREB1

(3) Induction of viral mimicry upon loss of DHX9 and ADAR1 in breast cancer cellsbioRxiv : the preprint server for biologyOctober 31, 2023Cottrell KA, Ryu S, Torres LS, Schab AM, Weber JD.mM Trolox and 1x HALT. Biotin labeling was verified by immunoblotting with HRP-streptavidin (Abcam, ab7403). Biotinylated proteins were purified using streptavidin magnetic beads (Thermo Fisher Scientific, 88816).

(4) TurboID reveals the proxiomes of Chlamydomonas proteins involved in thylakoid biogenesis and stress responsePlant PhysiologyOctober 26, 2023Elena Kreis, Katharina König, Melissa Misir, Justus Niemeyer, Frederik Sommer, Michael SchrodaTween-20 in PBS) at 22°C for 30 min. The blots were immersed in streptavidin-HRP (1:20,000 dilution, Abcam ab7403) or anti-Biotin-HRP (1:40,000, Sigma-Aldrich A0185) in biotin blocking buffer at 22°C for 60 min, then

(5) The late-evolving salmon and trout join the GnRH1 clubHistochemistry and Cell BiologyAugust 11, 2023Kristian R. von Schalburg, Brent E. Gowen, Kris A. Christensen, Eric H. Ignatz, Jennifer R. Hall, Matthew L. RisePBS-BSA for 1 h. After 3× PBS-BSA washes, the sections were exposed to streptavidin-peroxidase (Abcam ab7403) (1:500) for 30 min, then washed in pure PBS and immersed in DAB solution (DAB tetrahydrochloride, Sigma

(6) RETICULON-LIKE PROTEIN B2 is a pro-viral factor co-opted for the biogenesis of viral replication organelles in plantsThe Plant CellAugust 2, 2023Qianshen Zhang, Zhiyan Wen, Xin Zhang, Jiajie She, Xiaoling Wang, Zongyu Gao, Ruiqi Wang, Xiaofei Zhao, Zhen Su, Zhen Li, et al.blocked and incubated with the appropriate primary antibodies or streptavidin-HRP (Abcam, Cat. No. ab7403; 1:20,000 dilution), followed by incubation with corresponding secondary antibodies. Information about

(7) Placental deficiency of the (pro)renin receptor ((P)RR) reduces placental development and functional capacityFront Cell Dev BiolAugust 1, 2023Lachlan G. Schofield, Richard G. S. Kahl, Samantha L. Rodrigues, Joshua J. Fisher, Saije K. Endacott, Sarah J. Delforce, Eugenie R. Lumbers, Jacinta H. Martin, Kirsty G. Pringle1% BSA/PBS for 1 h at RT. Tertiary incubation of streptavidin-biotin-horseradish peroxidase complex (ab7403, Abcam) was then carried out for 1 h at RT before immunostaining was developed by incubation in 3,3′-diaminobenzidine

(8) Identification and functional validation of an enhancer variant in the 9p21.3 locus associated with glaucoma risk and elevated expression of p16 INK4abioRxivMay 25, 2023Yizhou Zhu, Cagdas Tazearslan, Michael G. Rosenfeld, Andras Fiser, Yousin Suhbuffer. The gel was then transferred to a nitrocellulose membrane and probed with streptavidin-HRP (Abcam ab7403) and imaged on exposure film (Thermo 34090) using ECL substrate(Thermo 32106). In silico calculation

(9) Macrophages regulate gastrointestinal motility through complement component 1qeLifeApril 26, 2023Mihir Pendse, Haley De Selle, Nguyen Vo, Gabriella Quinn, Chaitanya Dende, Yun Li, Cristine N Salinas, Tarun Srinivasan, Daniel C Propheter, Alexander A Crofts, et al.drug",Streptavidin-Cy5,Thermo Fisher,Cat# 434316, "Chemical compound, drug",Streptavidin-HRP conjugate,Abcam,Cat# ab7403,ELISA "Chemical compound, drug",Sylgard 184 Silicone Elastomer,Fisher Scientific,Cat# 4019862, "Chemical

(10) RNA localization mechanisms transcend cell morphologyeLifeMarch 3, 2023Goering R, Arora A, Pockalny MC, Taliaferro JM.times in PBST (PBS +0.01% Tween). Biotinylated RNA was then detected using streptavidin-HRP (Abcam ab7403) at a dilution of 1–20,000 in 3% BSA by addition to the membrane with rocking overnight at 4 °C. Membranes

(11) Serum MFAP4 , a novel potential biomarker for liver cirrhosis screening, correlates with transient elastography in NAFLD patientsJGH OpenMarch 1, 2023Reine Kanaan, Cesar Yaghi, Carole Saade Riachy, Anders Schlosser, Aline Hamade, Uffe Holmskov, Myrna Medlej‐Hashim, Grith Lykke Sørensen, Rania Jounblat(HG‐HYB 7–18) diluted 1:2000, horseradish peroxidase‐conjugated streptavidin diluted 1:10000 (Abcam, ab7403), and finally O‐phenylenediamine in citrate–phosphate buffer (pH 5 containing 0.03% H2O2) (Sigma–Aldrich).

(12) Fibril treatment changes protein interactions of tau and α-synuclein in human neuronsJournal of Biological ChemistryMarch 1, 2023Tagan A. Griffin, Paul D. Schnier, Elisa M. Cleveland, Robert W. Newberry, Julia Becker, George A. CarlsonTris-buffered saline plus Tween-20, probed with streptavidin-HRP (1:25,000 dilution; Abcam; catalog no.: ab7403) for 1 h, blocked with 10% fetal bovine serum, and then washed with Tris-buffered saline plus Tween-20

(13) TRIM21-dependent target protein ubiquitination mediates cell-free Trim-AwayCell reportsFebruary 28, 2023Mevissen TET, Prasad AV, Walter JC.unconjugated",Jackson ImmunoResearch,Cat# 211–005-109; RRID: AB_2339147 Streptavidin (HRP),Abcam,Cat# ab7403 Bacterial and virus strains,, E. coli OverExpress™C41(DE3) Chemically Competent Cells,Sigma,Cat# CMC0017 "Chemicals,

(14) Swietenine Alleviates Nonalcoholic Fatty Liver Disease in Diabetic Mice via Lipogenesis Inhibition and Antioxidant MechanismsAntioxidants (Basel)February 27, 2023Kit-Kay Mak, Shiming Zhang, Jestin Chellian, Zulkefeli Mohd, Ola Epemolu, Albena T. Dinkova-Kostova, Madhu Katyayani Balijepalli, Mallikarjuna Rao Pichikah, (15) washed with PBS, (16) incubated in the dark with Streptavidin-Horseradish Peroxidase (HRP, # ab7403, Abcam, Cambridge, UK) conjugates at 25 °C for 1 h, (17) washed with PBS, (18) supplemented with freshly

(15) TRIM24 controls induction of latent HIV-1 by stimulating transcriptional elongationCommunications biologyJanuary 23, 2023Horvath RM, Dahabieh M, Malcolm T, Sadowski I.CDK9 (1:4000)—Abcam ab239364; Cyclin T1 (1:500)—Santa Cruz sc-10750; streptavidin-HRP (1:40,000)—Abcam ab7403; goat anti-rabbit-HRP (1:2,000,000)—Abcam ab6721; goat anti-mouse-HRP (1:20,000)—Pierce #1858413. Immunoprecipitations

(16) Potential Protective Effect of Dengue NS1 Human Monoclonal Antibodies against Dengue and Zika Virus InfectionsBiomedicinesJanuary 16, 2023Rochanawan Sootichote, Wilarat Puangmanee, Surachet Benjathummarak, Siriporn Kowaboot, Atsushi Yamanaka, Korbporn Boonnak, Sumate Ampawong, Supawat Chatchen, Pongrama Ramasoota, Pannamthip PitaksajjakulVascular permeability was examined by refilling the top chamber with medium containing streptavidin-HRP (ab7403, Abcam, Cambridge, UK). After 5 min incubation, culture fluid from the lower chamber was collected,

(17) POU1F1/Pou1f1 c.143-83A > G Variant Disrupts the Branch Site in Pre-mRNA and Leads to DwarfismEndocrinologyDecember 19, 2022Akiba K, Hasegawa Y, Katoh-Fukui Y, Terao M, Takada S, Hasegawa T, Fukami M, Narumi S.antibody for 1 hour at room temperature. Streptavidin horseradish peroxidase (1:10,000; catalog no. ab7403; Abcam) was added to the sections and incubated for 30 minutes at room temperature. The sections were

(18) Prednisolone and mesenchymal stem cell preloading protect liver cell migration and mitigate extracellular matrix modification in transplanted decellularized rat liverStem Cell Research & TherapyDecember 1, 2022Atefeh Yaghoubi, Negar Azarpira, Saied Karbalay-Doust, Sajad Daneshi, Zahra Vojdani, Tahereh Talaei-Khozanidevelopment was done by treating the samples with 200–400 μL streptavidin-HRP (1:10,000; Abcam, USA; ab7403) followed by incubating in 3,3′-Diaminobenzidine tetrahydrochloride (Sigma Aldrich, D5905). Finally,

(19) Optimizing artificial meniscus by mechanical stimulation of the chondrocyte-laden acellular meniscus using ad hoc bioreactorStem Cell Research & TherapyDecember 1, 2022Mehri Shadi, Tahereh Talaei-Khozani, Mahsa Sani, Radmarz Hosseinie, Hossein Parsaei, Zahra Vojdani(1:250; ab6577) overnight at 4 °C. After washing with PBS, streptavidin-HRP (1:10,000; Abcam, USA; ab7403) was added to each section and incubated for 20–30 min at room temperature followed by incubating in

(20) Dynamic FMR1 granule phase switch instructed by m6A modification contributes to maternal RNA decayNature CommunicationsDecember 1, 2022Guoqiang Zhang et al.blocking buffer (Beyotime, GS009B) and subsequently incubated with Streptavidin HRP (1:5000; Abcam, ab7403) diluted by blocking buffer for 15 min. The membrane was washed with washing buffer (Beyotime, GS009W),

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Streptavidin (HRP) from Abcam

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Streptavidin (HRP) from Abcam

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